Microsatellite DNA technique was used to detect the genetic variation between five hatchery populations of the Pacific oyster from China and two wild populations from Japan. Seven microsatellite loci screened in this ...Microsatellite DNA technique was used to detect the genetic variation between five hatchery populations of the Pacific oyster from China and two wild populations from Japan. Seven microsatellite loci screened in this study showed high polymorphism in both hatchery and wild populations, as observed in an average number of allele per locus （19.1-29.9） and average expected heterozygosity （0.916-0.958）. No significant difference in average allelic richness or expected heterozygosity was observed between Chinese hatchery populations and Japanese wild populations. Pairwise Fsr values and heterogeneity tests of allele frequencies showed significant genetic differentiation between all populations. According to the neighbor-joining tree constructed on the basis of the Dc distance, the seven populations fell into three groups showing a clear division between hatchery and wild populations, and between the northern and southern hatchery populations. Assignment tests correctry assigned high percentages （97%-100%） of individuals to their original populations and demonstrated the feasibility of microsatellite analysis for discrimination between populations. The information obtained in this study is useful for designing suitable management guidelines and selective breeding programs for the Pacific oyster in China.展开更多
In this article, antibiogram and heavy metal resistance profile of bacteria isolated from giant freshwater prawn （Macrobrachium rosenbergii） hatchery in Malaysia are described. Although giant freshwater prawn was in...In this article, antibiogram and heavy metal resistance profile of bacteria isolated from giant freshwater prawn （Macrobrachium rosenbergii） hatchery in Malaysia are described. Although giant freshwater prawn was introduced into Malaysia since the 1980s, there was no database information on antibiogram and heavy metal resistance profile of bacteria from giant freshwater prawn （34. rosenbergii） hatchery in Malaysia. Therefore, this study was carried out to determine the effectiveness of antibiotic and heavy metal resistance profile to control bacterial diseases in M. rosenbergii hatchery. The results can provide valuable information for local M. rosenbergii post-larval producer. Antibiotic sensitivity test was carried out by disk-diffusion method against 15 types of antibiotics as follows： oxolinic acid （2 μg）, ampicillin （10μg）, erythromycin （15μg）, furazolidone （15 μg）, lincomycin （15μg）, amoxicillin （25 μg）, colistin sulphate （25 μg）, doxycycline （30μg）, florfenicol （30 μg）, flumequine （30 lag）, nalidixic acid （30μg）, tetracycline （30μg）, oleandomycin （15μg）, fosfomycin （50μg）, and spiramycin （100 μg）, whereas heavy metal resistance profile of the present bacterial isolates was determined by 2-fold agar dilution technique. In this study, 5 types of bacteria were successfully isolated; they were Aeromonas spp. （n = 77）, Escherichia coli （n = 73）, Edwardsiella spp. （n = 62）, Salmonella spp. （n = 75）, and Vibrio spp. （n = 43）. The result showed that furazolidone was the most effective antibiotic to control the bacteria isolated in this study, approximately 89.7% of the bacterial isolates were sensitive to this antibiotic. Multiple antibiotic resistance （MAR） index indicated that the hatchery water source and M. rosenbergii post-larval and sediment tanks were at high-risk exposure to the tested antibiotic. Furthermore, all the tested heavy metals （Cd2＋, Cr6＋, Hg2＋, and Cu2＋） failed to inhibit the 展开更多
Hatchery contamination can result in ingested or inhaled microbes that may modify colonization of the intestinal and respiratory tract, with potential to influence early growth, inflammation, and overall health. Six e...Hatchery contamination can result in ingested or inhaled microbes that may modify colonization of the intestinal and respiratory tract, with potential to influence early growth, inflammation, and overall health. Six experiments were completed to compare chick quality, inflammation, and health between two hatcheries (H1 and H2). On embryonic d0, 45 eggs from the same breeder flock were set at each hatchery. On d0, length, abdominal height, navel and leg abnormalities, and self-righting were measured for 36 chicks/hatchery, yolk sacs were weighed, and crop/cloaca swabs were cultured from 12 chicks/hatchery. On d7, mid-ileum and ceca were cultured from 12 chicks/hatchery. On d0 and d7, body weight (BW) and intestinal weight were measured, lung/air sac swabs and liver were cultured, and liver and air sacs were scored for health. Blood was collected on d0 and d7 for serum alpha-1-acid glycoprotein concentrations in Exp 1 and 2. Data was analyzed using Student’s t-test or χ<sup>2</sup>, significance p < 0.05. On d0, BW, length, yolk free BW, and intestinal weight were greater for H2 chicks (p < 0.05). Liver bacterial recovery was decreased in H2 on d0 (p < 0.05) and there were fewer average leg and righting abnormalities in H2 (p < 0.05). Decreased lactase positive Enterobacteriaceae were noted in H2 in crop/cloaca and lung/air sac swabs (p < 0.05), and of alpha and beta hemolysis in crop/cloaca swabs, and alpha and gamma hemolysis in lung/air sac swabs (p < 0.05) on d0. By d7, only alpha hemolytic bacteria were increased in lung/air sac swabs of H2. Based on factors measured, chicks from H2 showed favorable microbial colonization, starting quality, and improved health on d0. While not sustained through d7, differences in d0 microbial recovery may have shifted microbial development and potentially influenced immune response development. These experiments elucidated the importance of hatchery environment on early chick quality, microbial colonization, overall inflammation, and chick health.展开更多
The paper presents the results of the sterlet sturgeon Acipenser ruthenus reintroduction into the Kama Reservoir(Perm Krai,Russia)by sterlet fry from hatcheries into the reservoir since 2001.The effectiveness of hatch...The paper presents the results of the sterlet sturgeon Acipenser ruthenus reintroduction into the Kama Reservoir(Perm Krai,Russia)by sterlet fry from hatcheries into the reservoir since 2001.The effectiveness of hatchery releases was evaluated by monitoring gillnet sampling 2014-2020.The results showed that the species was reported throughout entire Kama Reservoir,which contrasts with 1988-1994 data,when the species was registered only at the upper part of the reservoir near the Kama River mouth.The highest number and biomass values of catch per unit effort(CPUE)of the sterlet were found in submerged river channels-the deepest habitats with flow conditions which are close to the ecological requirements of the species.The distribution of the sterlet within the channel biotopes of the reservoir is defined by reduced variability.The biggest individuals and the widest size range of the sterlet were typical for the upper area of the reservoir and Chusovskoy Bay,which have the greatest flow.The smallest sizes were typical for fish from catches near the dam of the reservoir,which is consistent with the published data on the distribution of sterlet in reservoir conditions.The presented results may indicate the artificial formation of the sterlet stock in the Kama Reservoir maintained by hatchery releases.The revealed distribution of the sterlet in the reservoir determines the direction of further research on natural reproduction and thus formation of an artificially formed population.Also,future studies are required to assess the possibility of hybridization of the sterlet of artificial origin with natural populations of the species,since the low genetic diversity of the sterlet released into the Kama Reservoir which can lead to irreversible loss of genetic individuality of the natural stocks of the species in the catchment of upper course of the Kama River.展开更多
We estimated the proportion of hatchery and natural fall spawning chum salmon returning to the Amur River using chemical markers specific to hatchery-origin fry.We used otolith microchemistry technique to identify fis...We estimated the proportion of hatchery and natural fall spawning chum salmon returning to the Amur River using chemical markers specific to hatchery-origin fry.We used otolith microchemistry technique to identify fish with artificial origin among returning spawners.First,we found that juveniles of artificial origin had higher values of the Sr:Ca molar ratio of the otoliths’edge zone compared with juveniles of natural origin,what can be related to the use of rearing feed produced from raw materials of marine origin rich in strontium.Then we observed that most of the spawners from Anyuisky Hatchery and from the Amur River mouth at the start of the spawning migration has also the higher value of Sr:Ca molar ratio of the juvenile zone of otoliths.Also,adults with higher values of the Sr:Ca molar ratio are characterized by a skewed right in the peak of the age distribution.Both,the age structure and phenological shift in the time of spawning migration of individuals with higher value of the used chemical marker corresponds to results of studies on hatchery-produced chum salmon completed at different parts on Northern Pacific.The results of this study will be used in the management of Amur fall chum salmon fisheries,and also demonstrates the necessity of the development of specific measures for increasing the survival of juvenile anadromous salmonids released at large rivers and exposed to prolonged freshwater migration to the ocean.As a further application of the methodology,we plan to identify the markers specific to each of the hatcheries and main spawning tributaries belonging to Amur River catchments.This will be an important step in the evaluation of the contribution of different stocks in mixed fisheries and also in the estimation of the effect of hatchery releases on naturally spawning stocks of Amur fall chum.Following to,our results may indicate the applicability of this approach for the determination of artificial-origin fish in a mixed sample of the Amur fall chum salmon.展开更多
Purple Non-Sulfur Bacteria (PNSB), also known as phototrophic bacteria are widely distributed in both freshwater and marine environment and capable to <span><span style="font-family:Verdana;">gro...Purple Non-Sulfur Bacteria (PNSB), also known as phototrophic bacteria are widely distributed in both freshwater and marine environment and capable to <span><span style="font-family:Verdana;">grow in wide range of substrates. In this study, Bacterium </span><i><span style="font-family:Verdana;">Rhodobacter</span></i></span><i><span style="font-family:Verdana;"> sphaeroides</span></i><span style="font-family:Verdana;"> strain UMS2, a freshwater isolate was used in this study in utilization of fish hatchery waste. This study was conducted to determine the nutritional values of bioprocess product that was grown in fish hatchery waste. Finally, the waste bio-converted product was used as feed supplement to monitor the </span><span><span style="font-family:Verdana;">growth performance of live feed </span><i><span style="font-family:Verdana;">Tubifex</span></i><span style="font-family:Verdana;"> spp. Inoculum of </span><i><span style="font-family:Verdana;">Rhodobacter</span></i></span><i><span style="font-family:Verdana;"> sphaeroides</span></i><span style="font-family:Verdana;"> strain UMS2 was developed in 112 synthetic media and 48-h culture of 30% (v/v) inoculum was used in fish hatchery waste during the bioprocess. The nutritional values of bio-converted product, except total ash (%), were not significantly improved with 30% (v/v) inoculum of </span><i><span style="font-family:Verdana;">Rhodobacter sphaeroides</span></i><span style="font-family:Verdana;">, strain UMS2. Feeding trial in bloodworm (</span><i><span style="font-family:Verdana;">Tubifex</span></i><span style="font-family:Verdana;"> spp.) with bioconversion product conducted for 15 days to monitor growth (w/v) of live feed. Initial growth 1.42 ± 0.001 g/L of </span><i><span style="font-family:Verdana;">Tubifex</span></i><span style="font-family:Verdana;"> spp. was stocked in 15 × 75 × 15 cm plastic tray connected with recirculated system. </span><i><span style="font-family:Verdana;">Tubifex</span></i><span style="font-family:Verdana;"> spp. was obser展开更多
Structures separating fish hatchery ponds from open spring water sources must restrict fish movement from the pond to the spring, allow for constant water flow, and potentially reduce incoming spring water gas supersa...Structures separating fish hatchery ponds from open spring water sources must restrict fish movement from the pond to the spring, allow for constant water flow, and potentially reduce incoming spring water gas supersaturation. This article describes a novel inlet structure that fulfills those requirements. In addition, it requires minimal maintenance, and allows for the quick and easy removal of debris in the event of partial plugging. This simple aluminum structure consists of a mount for attachment to the inflow pipe and a terminal splash plate with aeration holes. The splash plate is perpendicular to the water flow during normal operations to both prevent fish from jumping into the pipe and aerate the incoming water. However, it can be easily swiveled upward for the efficient removal of debris. Use of the inlet structure consistently decreased gas supersaturation in the spring water as it entered the fish production pond. By decreasing gas levels and maintaining water flows, potentially hazardous fish health issues can be avoided by using this structure. In addition, this relatively inexpensive and simple device will greatly reduce the labor required for removing debris compared to traditional screens.展开更多
Commercial fish hatchery generates waste both organic and inorganic;the sources are primarily from uneaten food and fish feces. Conventional methods of treating hatchery wastes will increase the operating cost and bec...Commercial fish hatchery generates waste both organic and inorganic;the sources are primarily from uneaten food and fish feces. Conventional methods of treating hatchery wastes will increase the operating cost and become extra burden in production. It is necessary to develop a new research application of this nonconventional resource and reduce the negative impacts of hatchery waste on the environment. The whole project is to utilize hatchery waste through bioprocess for probiotic fortified live feed production. In this study, the chemical composition of hatchery waste was determined to understand the suitability waste to get value-added derived products through bioprocess. Composite samples were collected everyday and dried in an oven at a temperature of 65°C until complete dryness. Dried samples were mixed well and grinded into fine powder. The analytical parameters like total solids, ammonium nitrogen, nitrite, nitrate and phosphate were determined from the freshly collected samples. Total nitrogen, total phosphorus and total potassium were determined from the dry samples. Total solids, ammonium nitrogen, nitrite, nitrate and phosphate-phosphorus were observed in the ranged from 75 - 82 mg/L, 0.25 - 8.5 mg/L, 0.05 - 1.9 mg/L, 0.04 - 6.7 mg/L and 4.1 - 16.7 mg/L respectively. On the other hand, the mean content of 3.75% total nitrogen, 1.80% total phosphorus and 0.15% potassium were determined in dry hatchery wastes. The analytical parameters are useful and demonstrate that the nutrients in both fresh and dry waste will be supportive for the growth of microbes in the bioprocess system.展开更多
Occupational noise is commonly encountered during aquaculture. This study documented noise levels in two buildings at a production fish hatchery, a tank room with 32, 1.8-m diameter tanks, and a rearing pavilion with ...Occupational noise is commonly encountered during aquaculture. This study documented noise levels in two buildings at a production fish hatchery, a tank room with 32, 1.8-m diameter tanks, and a rearing pavilion with 32, 6.1-m diameter tanks. With water flowing to all of the tanks in the tank room, mean noise levels were 68.4 dB, and significantly increased to 73.0 dB during tank cleaning and 73.2 dB when intermittent automatic feeders were running. The highest tank room values of 77.1 dB were recorded directly next to individual tanks during cleaning. With water flowing to all of the tanks in the rearing pavilion, mean noise levels were 70.2 dB. A significant increase to 76.1 dB was observed when the pavilion tanks were being power washed, with the highest value of 83.2 dB recorded immediately adjacent to the power washer. Although none of the noise levels exceeded regulatory limits, the use of techniques to reduce occupational noise in aquaculture environments is recommended.展开更多
This study provides new insights for the hatchery released Chinese shrimp(Fenneropenaeus chinensis),including proportion,dynamic migration route,after they were released into nature for stock enhancement using a new s...This study provides new insights for the hatchery released Chinese shrimp(Fenneropenaeus chinensis),including proportion,dynamic migration route,after they were released into nature for stock enhancement using a new strategy quite different than ever.Chinese shrimp were sampled at 22 survey stations during two investigation voyages acrossing 74 survey stations in the Bohai Sea from July 16 to August 9 in 2015.Among 289 sampled individuals during the second voyage,totally 155 shrimps were identified as hatchery shrimp released into the Laizhou Bay at mid-May in 2015 based on finger-print of eight SSR(simple sequence repeats)markers,and the proportion of hatchery released shrimp in recapture samples were from 41.30%–85.71%in each station with an average value 53.63%,which verified a previous view point that up to 90%of autumn season Chinese shrimp landing in the Bohai Sea were composed of hatchery released.Meanwhile,the dynamic migration route of hatchery released shrimp revealed that part of released shrimp migrated heading northwest along the west coast of the Bohai Sea up to the Bohai Bay but just remained at the Laizhou Bay until over-wintering migration at midOctober when they initiate over-wintering migration.Present unnatural spring season shrimp fishing model cut the throat of spawner shrimp chance to swim back to their respective spawning plants at each spring,it still no chance to clarify whether the hatchery released shrimp could replenish to the reproduce population and complete a whole life cycle as same as their natural relatives.展开更多
Mud crab is an important economic crab cultured in Pacific and Indian Ocean Rim. Based on the knowledge about the characteristic requirements in seedling cultivation of mud crab under high-temperature conditions, this...Mud crab is an important economic crab cultured in Pacific and Indian Ocean Rim. Based on the knowledge about the characteristic requirements in seedling cultivation of mud crab under high-temperature conditions, this article reviews and discusses major factors affecting seedling rearing of mud crabs, and presents novel hatchery facilities for improvement of seedling rearing. The results of our study showed that major factors affecting seedling cultivation of mud crabs included： （1） the micro-ecological environment and characteristics of water quality in seedling rearing ponds, and a double-bottom cultivation facilities and cultivation techniques that meet the requirement; （2） salinity, temperature, light, NOE-N, NH4-N and other physical and chemical environmental factors that affect survival probability ofzoaea; （3） the total number of bacteria in the zoaea-culturing pond and the number of vibrio in seedling rearing; and （4） nutritional intensification with bait organisms in zoaea-culturing. To meet and improve these conditions, a novel hatchery facility was designed to modify the pond roof in accordance with the characteristics of the zoaea stage, and improve natural light thermostat control in seedling cultivation of mud crabs.展开更多
基金the Ministry of Education (No. NCET-04-0640)the National Natural Science Foundation of China (No. 30571442).
文摘Microsatellite DNA technique was used to detect the genetic variation between five hatchery populations of the Pacific oyster from China and two wild populations from Japan. Seven microsatellite loci screened in this study showed high polymorphism in both hatchery and wild populations, as observed in an average number of allele per locus （19.1-29.9） and average expected heterozygosity （0.916-0.958）. No significant difference in average allelic richness or expected heterozygosity was observed between Chinese hatchery populations and Japanese wild populations. Pairwise Fsr values and heterogeneity tests of allele frequencies showed significant genetic differentiation between all populations. According to the neighbor-joining tree constructed on the basis of the Dc distance, the seven populations fell into three groups showing a clear division between hatchery and wild populations, and between the northern and southern hatchery populations. Assignment tests correctry assigned high percentages （97%-100%） of individuals to their original populations and demonstrated the feasibility of microsatellite analysis for discrimination between populations. The information obtained in this study is useful for designing suitable management guidelines and selective breeding programs for the Pacific oyster in China.
基金funded by Universiti Malaysia Terengganu and Ministry of Science, Technology and Innovation (MOSTI), Malaysia
文摘In this article, antibiogram and heavy metal resistance profile of bacteria isolated from giant freshwater prawn （Macrobrachium rosenbergii） hatchery in Malaysia are described. Although giant freshwater prawn was introduced into Malaysia since the 1980s, there was no database information on antibiogram and heavy metal resistance profile of bacteria from giant freshwater prawn （34. rosenbergii） hatchery in Malaysia. Therefore, this study was carried out to determine the effectiveness of antibiotic and heavy metal resistance profile to control bacterial diseases in M. rosenbergii hatchery. The results can provide valuable information for local M. rosenbergii post-larval producer. Antibiotic sensitivity test was carried out by disk-diffusion method against 15 types of antibiotics as follows： oxolinic acid （2 μg）, ampicillin （10μg）, erythromycin （15μg）, furazolidone （15 μg）, lincomycin （15μg）, amoxicillin （25 μg）, colistin sulphate （25 μg）, doxycycline （30μg）, florfenicol （30 μg）, flumequine （30 lag）, nalidixic acid （30μg）, tetracycline （30μg）, oleandomycin （15μg）, fosfomycin （50μg）, and spiramycin （100 μg）, whereas heavy metal resistance profile of the present bacterial isolates was determined by 2-fold agar dilution technique. In this study, 5 types of bacteria were successfully isolated; they were Aeromonas spp. （n = 77）, Escherichia coli （n = 73）, Edwardsiella spp. （n = 62）, Salmonella spp. （n = 75）, and Vibrio spp. （n = 43）. The result showed that furazolidone was the most effective antibiotic to control the bacteria isolated in this study, approximately 89.7% of the bacterial isolates were sensitive to this antibiotic. Multiple antibiotic resistance （MAR） index indicated that the hatchery water source and M. rosenbergii post-larval and sediment tanks were at high-risk exposure to the tested antibiotic. Furthermore, all the tested heavy metals （Cd2＋, Cr6＋, Hg2＋, and Cu2＋） failed to inhibit the
文摘Hatchery contamination can result in ingested or inhaled microbes that may modify colonization of the intestinal and respiratory tract, with potential to influence early growth, inflammation, and overall health. Six experiments were completed to compare chick quality, inflammation, and health between two hatcheries (H1 and H2). On embryonic d0, 45 eggs from the same breeder flock were set at each hatchery. On d0, length, abdominal height, navel and leg abnormalities, and self-righting were measured for 36 chicks/hatchery, yolk sacs were weighed, and crop/cloaca swabs were cultured from 12 chicks/hatchery. On d7, mid-ileum and ceca were cultured from 12 chicks/hatchery. On d0 and d7, body weight (BW) and intestinal weight were measured, lung/air sac swabs and liver were cultured, and liver and air sacs were scored for health. Blood was collected on d0 and d7 for serum alpha-1-acid glycoprotein concentrations in Exp 1 and 2. Data was analyzed using Student’s t-test or χ<sup>2</sup>, significance p < 0.05. On d0, BW, length, yolk free BW, and intestinal weight were greater for H2 chicks (p < 0.05). Liver bacterial recovery was decreased in H2 on d0 (p < 0.05) and there were fewer average leg and righting abnormalities in H2 (p < 0.05). Decreased lactase positive Enterobacteriaceae were noted in H2 in crop/cloaca and lung/air sac swabs (p < 0.05), and of alpha and beta hemolysis in crop/cloaca swabs, and alpha and gamma hemolysis in lung/air sac swabs (p < 0.05) on d0. By d7, only alpha hemolytic bacteria were increased in lung/air sac swabs of H2. Based on factors measured, chicks from H2 showed favorable microbial colonization, starting quality, and improved health on d0. While not sustained through d7, differences in d0 microbial recovery may have shifted microbial development and potentially influenced immune response development. These experiments elucidated the importance of hatchery environment on early chick quality, microbial colonization, overall inflammation, and chick health.
文摘The paper presents the results of the sterlet sturgeon Acipenser ruthenus reintroduction into the Kama Reservoir(Perm Krai,Russia)by sterlet fry from hatcheries into the reservoir since 2001.The effectiveness of hatchery releases was evaluated by monitoring gillnet sampling 2014-2020.The results showed that the species was reported throughout entire Kama Reservoir,which contrasts with 1988-1994 data,when the species was registered only at the upper part of the reservoir near the Kama River mouth.The highest number and biomass values of catch per unit effort(CPUE)of the sterlet were found in submerged river channels-the deepest habitats with flow conditions which are close to the ecological requirements of the species.The distribution of the sterlet within the channel biotopes of the reservoir is defined by reduced variability.The biggest individuals and the widest size range of the sterlet were typical for the upper area of the reservoir and Chusovskoy Bay,which have the greatest flow.The smallest sizes were typical for fish from catches near the dam of the reservoir,which is consistent with the published data on the distribution of sterlet in reservoir conditions.The presented results may indicate the artificial formation of the sterlet stock in the Kama Reservoir maintained by hatchery releases.The revealed distribution of the sterlet in the reservoir determines the direction of further research on natural reproduction and thus formation of an artificially formed population.Also,future studies are required to assess the possibility of hybridization of the sterlet of artificial origin with natural populations of the species,since the low genetic diversity of the sterlet released into the Kama Reservoir which can lead to irreversible loss of genetic individuality of the natural stocks of the species in the catchment of upper course of the Kama River.
基金support of the grant of the Ministry of Science and Higher Education of the Russian Federation project No.2019-0858"Biogeochemical and geochemical studies of landscapes in the conditions of the development of mineral deposits,the search for new methods of monitoring and forecasting the State of the environment".
文摘We estimated the proportion of hatchery and natural fall spawning chum salmon returning to the Amur River using chemical markers specific to hatchery-origin fry.We used otolith microchemistry technique to identify fish with artificial origin among returning spawners.First,we found that juveniles of artificial origin had higher values of the Sr:Ca molar ratio of the otoliths’edge zone compared with juveniles of natural origin,what can be related to the use of rearing feed produced from raw materials of marine origin rich in strontium.Then we observed that most of the spawners from Anyuisky Hatchery and from the Amur River mouth at the start of the spawning migration has also the higher value of Sr:Ca molar ratio of the juvenile zone of otoliths.Also,adults with higher values of the Sr:Ca molar ratio are characterized by a skewed right in the peak of the age distribution.Both,the age structure and phenological shift in the time of spawning migration of individuals with higher value of the used chemical marker corresponds to results of studies on hatchery-produced chum salmon completed at different parts on Northern Pacific.The results of this study will be used in the management of Amur fall chum salmon fisheries,and also demonstrates the necessity of the development of specific measures for increasing the survival of juvenile anadromous salmonids released at large rivers and exposed to prolonged freshwater migration to the ocean.As a further application of the methodology,we plan to identify the markers specific to each of the hatcheries and main spawning tributaries belonging to Amur River catchments.This will be an important step in the evaluation of the contribution of different stocks in mixed fisheries and also in the estimation of the effect of hatchery releases on naturally spawning stocks of Amur fall chum.Following to,our results may indicate the applicability of this approach for the determination of artificial-origin fish in a mixed sample of the Amur fall chum salmon.
文摘Purple Non-Sulfur Bacteria (PNSB), also known as phototrophic bacteria are widely distributed in both freshwater and marine environment and capable to <span><span style="font-family:Verdana;">grow in wide range of substrates. In this study, Bacterium </span><i><span style="font-family:Verdana;">Rhodobacter</span></i></span><i><span style="font-family:Verdana;"> sphaeroides</span></i><span style="font-family:Verdana;"> strain UMS2, a freshwater isolate was used in this study in utilization of fish hatchery waste. This study was conducted to determine the nutritional values of bioprocess product that was grown in fish hatchery waste. Finally, the waste bio-converted product was used as feed supplement to monitor the </span><span><span style="font-family:Verdana;">growth performance of live feed </span><i><span style="font-family:Verdana;">Tubifex</span></i><span style="font-family:Verdana;"> spp. Inoculum of </span><i><span style="font-family:Verdana;">Rhodobacter</span></i></span><i><span style="font-family:Verdana;"> sphaeroides</span></i><span style="font-family:Verdana;"> strain UMS2 was developed in 112 synthetic media and 48-h culture of 30% (v/v) inoculum was used in fish hatchery waste during the bioprocess. The nutritional values of bio-converted product, except total ash (%), were not significantly improved with 30% (v/v) inoculum of </span><i><span style="font-family:Verdana;">Rhodobacter sphaeroides</span></i><span style="font-family:Verdana;">, strain UMS2. Feeding trial in bloodworm (</span><i><span style="font-family:Verdana;">Tubifex</span></i><span style="font-family:Verdana;"> spp.) with bioconversion product conducted for 15 days to monitor growth (w/v) of live feed. Initial growth 1.42 ± 0.001 g/L of </span><i><span style="font-family:Verdana;">Tubifex</span></i><span style="font-family:Verdana;"> spp. was stocked in 15 × 75 × 15 cm plastic tray connected with recirculated system. </span><i><span style="font-family:Verdana;">Tubifex</span></i><span style="font-family:Verdana;"> spp. was obser
文摘Structures separating fish hatchery ponds from open spring water sources must restrict fish movement from the pond to the spring, allow for constant water flow, and potentially reduce incoming spring water gas supersaturation. This article describes a novel inlet structure that fulfills those requirements. In addition, it requires minimal maintenance, and allows for the quick and easy removal of debris in the event of partial plugging. This simple aluminum structure consists of a mount for attachment to the inflow pipe and a terminal splash plate with aeration holes. The splash plate is perpendicular to the water flow during normal operations to both prevent fish from jumping into the pipe and aerate the incoming water. However, it can be easily swiveled upward for the efficient removal of debris. Use of the inlet structure consistently decreased gas supersaturation in the spring water as it entered the fish production pond. By decreasing gas levels and maintaining water flows, potentially hazardous fish health issues can be avoided by using this structure. In addition, this relatively inexpensive and simple device will greatly reduce the labor required for removing debris compared to traditional screens.
文摘Commercial fish hatchery generates waste both organic and inorganic;the sources are primarily from uneaten food and fish feces. Conventional methods of treating hatchery wastes will increase the operating cost and become extra burden in production. It is necessary to develop a new research application of this nonconventional resource and reduce the negative impacts of hatchery waste on the environment. The whole project is to utilize hatchery waste through bioprocess for probiotic fortified live feed production. In this study, the chemical composition of hatchery waste was determined to understand the suitability waste to get value-added derived products through bioprocess. Composite samples were collected everyday and dried in an oven at a temperature of 65°C until complete dryness. Dried samples were mixed well and grinded into fine powder. The analytical parameters like total solids, ammonium nitrogen, nitrite, nitrate and phosphate were determined from the freshly collected samples. Total nitrogen, total phosphorus and total potassium were determined from the dry samples. Total solids, ammonium nitrogen, nitrite, nitrate and phosphate-phosphorus were observed in the ranged from 75 - 82 mg/L, 0.25 - 8.5 mg/L, 0.05 - 1.9 mg/L, 0.04 - 6.7 mg/L and 4.1 - 16.7 mg/L respectively. On the other hand, the mean content of 3.75% total nitrogen, 1.80% total phosphorus and 0.15% potassium were determined in dry hatchery wastes. The analytical parameters are useful and demonstrate that the nutrients in both fresh and dry waste will be supportive for the growth of microbes in the bioprocess system.
文摘Occupational noise is commonly encountered during aquaculture. This study documented noise levels in two buildings at a production fish hatchery, a tank room with 32, 1.8-m diameter tanks, and a rearing pavilion with 32, 6.1-m diameter tanks. With water flowing to all of the tanks in the tank room, mean noise levels were 68.4 dB, and significantly increased to 73.0 dB during tank cleaning and 73.2 dB when intermittent automatic feeders were running. The highest tank room values of 77.1 dB were recorded directly next to individual tanks during cleaning. With water flowing to all of the tanks in the rearing pavilion, mean noise levels were 70.2 dB. A significant increase to 76.1 dB was observed when the pavilion tanks were being power washed, with the highest value of 83.2 dB recorded immediately adjacent to the power washer. Although none of the noise levels exceeded regulatory limits, the use of techniques to reduce occupational noise in aquaculture environments is recommended.
基金The National Key R&D Program of China under contract No.2017YFE0104400the National Basic Research Program(973 Program)of China under contract No.2015CB453303
文摘This study provides new insights for the hatchery released Chinese shrimp(Fenneropenaeus chinensis),including proportion,dynamic migration route,after they were released into nature for stock enhancement using a new strategy quite different than ever.Chinese shrimp were sampled at 22 survey stations during two investigation voyages acrossing 74 survey stations in the Bohai Sea from July 16 to August 9 in 2015.Among 289 sampled individuals during the second voyage,totally 155 shrimps were identified as hatchery shrimp released into the Laizhou Bay at mid-May in 2015 based on finger-print of eight SSR(simple sequence repeats)markers,and the proportion of hatchery released shrimp in recapture samples were from 41.30%–85.71%in each station with an average value 53.63%,which verified a previous view point that up to 90%of autumn season Chinese shrimp landing in the Bohai Sea were composed of hatchery released.Meanwhile,the dynamic migration route of hatchery released shrimp revealed that part of released shrimp migrated heading northwest along the west coast of the Bohai Sea up to the Bohai Bay but just remained at the Laizhou Bay until over-wintering migration at midOctober when they initiate over-wintering migration.Present unnatural spring season shrimp fishing model cut the throat of spawner shrimp chance to swim back to their respective spawning plants at each spring,it still no chance to clarify whether the hatchery released shrimp could replenish to the reproduce population and complete a whole life cycle as same as their natural relatives.
文摘Mud crab is an important economic crab cultured in Pacific and Indian Ocean Rim. Based on the knowledge about the characteristic requirements in seedling cultivation of mud crab under high-temperature conditions, this article reviews and discusses major factors affecting seedling rearing of mud crabs, and presents novel hatchery facilities for improvement of seedling rearing. The results of our study showed that major factors affecting seedling cultivation of mud crabs included： （1） the micro-ecological environment and characteristics of water quality in seedling rearing ponds, and a double-bottom cultivation facilities and cultivation techniques that meet the requirement; （2） salinity, temperature, light, NOE-N, NH4-N and other physical and chemical environmental factors that affect survival probability ofzoaea; （3） the total number of bacteria in the zoaea-culturing pond and the number of vibrio in seedling rearing; and （4） nutritional intensification with bait organisms in zoaea-culturing. To meet and improve these conditions, a novel hatchery facility was designed to modify the pond roof in accordance with the characteristics of the zoaea stage, and improve natural light thermostat control in seedling cultivation of mud crabs.
