Objective:To investigate the antidiabetic properties of aqueous extract of stem bark of Afzelia africana(A.africana)and its beneficial effect on haematological parameters in streptozotocin induced diabetic rats.Method...Objective:To investigate the antidiabetic properties of aqueous extract of stem bark of Afzelia africana(A.africana)and its beneficial effect on haematological parameters in streptozotocin induced diabetic rats.Methods:A total of 30 rats including 24 diabetic and 6 normal rats were used for this study.Diabetes was induced in male Wistar rats by intraperitoneal injection of streptozotocin.After being confirmed diabetic,animals were orally treated with distilled water or extracts at 100 or 200 mg/kg body weight daily for 10 days.The haematological parameters including red blood and white blood cells and their functional indices were evaluated in diabetic treated groups compared with the controls.Results:The extract significantly reduced the blood glucose levels while the best result was obtained at 200 mg/kg body weight The feed and water intake in diabetic rats were significantly reduced while weight loss was minimized at both dosages.Similarly,the levels of red blood,white blood cells and their functional indices were significantly improved after extract administration at both doses.Conclusions:It can be concluded that the aqueous extract of bark of A.africana possesses antihyperglycemic properties.In addition,the extract can prevent various complications of diabetes and improve some haematological parameters.Further experimental investigation is needed to exploit its relevant therapeutic effect to substantiate its ethnomedicinal usage.展开更多
Background: Adequate selection of a prospective whole blood donor protects his health and safety of the recipient. Objectives: The main objective of this study was to determine the haematology parameters of apparently...Background: Adequate selection of a prospective whole blood donor protects his health and safety of the recipient. Objectives: The main objective of this study was to determine the haematology parameters of apparently healthy prospective whole blood donors. Participants and Methods: This was a hospital based prospective study carried out from August to October 2020 at the blood transfusion unit of the Lagos State University Teaching Hospital (LASUTH), Ikeja, Nigeria. A structured pretested questionnaire was used for data collection. The socio demographic status and the haematology parameters of apparently healthy prospective whole blood donors who tested negative for HIV, hepatitis B and C markers were captured. Obtained data were analysed with the statistical package for the social scientist software version 20. Results: One hundred male (97.1%) and three female (2.9%) apparently healthy prospective whole blood donors were studied. The median age of study subjects was 30 years. Obtained median haematology parameter values were 13 g/dl, 40%, 4.9/nl and 203.9/nl for haemoglobin concentration, haematocrit, total white cell and platelet counts respectively. The median values for the mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) and mean corpuscular volume (MCV) of participants were 32.6 g/dl, 27.7 pg and 85.7 fl respectively. Observed prevalence of subnormal haematology parameters for haemoglobin concentration, total white cells, platelets were 12.6%, 25.2%, and 13.6% respectively. Also subnormal values for MCHC, MCH, MCV were 11.7%, 26.2%, and 16.5% respectively among prospective whole blood donors in this study. No higher than normal haematology parameter values were observed. Median values for erythrocyte sedimentation rate was 8.4 mm/hr. Conclusion: A significant percentage of apparently healthy prospective whole blood donors had subnormal haematology parameters values. Obtained normal values in our study are comparable with local reference range reports from previous stud展开更多
Objective:To evaluate clinical and hematological aspects of dogs naturally infected with Hepatozoon canis(H.canis)presented at the Small Animal Clinics of Guru Angad Dev Veterinary and Animal Sciences University,Ludhi...Objective:To evaluate clinical and hematological aspects of dogs naturally infected with Hepatozoon canis(H.canis)presented at the Small Animal Clinics of Guru Angad Dev Veterinary and Animal Sciences University,Ludhiana.Methods:Blood films of 34 naturally infected dogs were examined for haematological alterations and parasitaemia.Signalment and clinical signs were recorded from the animals.Clinical histories were filled oul during the consultation.Results:Of the 34 positive dogs by Giemsa stained peripheral blood films.88.23%presented parasilaemia by H.canis only,while 11.77%had the combination of H.canis,Babesia sp.and Ehrlichia sp.Young male dogs less than oue-year-old,of nondescript breed,were the most commonly affected.And 26.47%were presented with anorexia/inappetence as the only clinical symptom.Other clinical symptoms were mild to moderate fever,pale mucosae and lethargy;a few were also showing the signs of vomiting and diarrhoea.Haematological alterations showed mainly normochromic-normocytic anaemia,leukocytosis and neutrophilia.Conclusions:The findings of this study substantiate that H.canis caused clinical and haematological alterations of the varied intensity in dogs,even with low parasilaemia.should he taken into consideration.展开更多
Objective:Voriconazole(VCZ)is a triazole antifungal agent widely used in immunocompromised patients with suspected or proven invasive fungal infections.The achievement of therapeutic range(1-5 mg/L)is essential to max...Objective:Voriconazole(VCZ)is a triazole antifungal agent widely used in immunocompromised patients with suspected or proven invasive fungal infections.The achievement of therapeutic range(1-5 mg/L)is essential to maximize VCZ efficacy,as its pharmacokinetics is characterized by a wide inter-and intra-individual variability.This study aims to quantify the variability of VCZ trough concentrations in children and adolescents with haematological diseases and optimize therapeutic drug monitoring in clinical practice.Methods:We analysed the monitoring concentrations of all children(<18 years old)treated with VCZ in the Haematology Department of Robert DebréHospital between January 2014 and December 2016.Demographic,clinical data,and VCZ dosing and monitoring concentrations measured by high-performance liquid chromatography with ultraviolet detection(HPLC-UV)were analysed.Non-parametric tests were performed using SPSS IBM 24.0.Results:380 trough VCZ concentrations at steady-state(Ctrough,ss)were available in 79 children:45.6%had first Ctrough,ss in the therapeutic range at first monitoring,46.8%had Ctrough,ss below 1 mg/L and 7.6%had Ctrough,ss over 5 mg/L.Forty-one patients were treated with recommended doses but only 53%of them reached the therapeutic range.There was no impact of age,sex,biological parameters,or indication of VCZ on Ctrough,ss values.The number of Ctrough,ss in the therapeutic range increases with the number of monitoring per patient following dosage adaptations.Conclusion:The wide inter-and intra-individual variability of VCZ trough concentrations at recommended doses confirm the need to standardize VCZ monitoring and identify factors to be considered to prospectively adapt treatment for each patient.展开更多
The reduced efficiency of antibiotic growth promoters in livestock, and the unavoidable residual effects on livestock products such as egg, meat and milk have pushed researchers to rush towards the use of phytobiotics...The reduced efficiency of antibiotic growth promoters in livestock, and the unavoidable residual effects on livestock products such as egg, meat and milk have pushed researchers to rush towards the use of phytobiotics in animal feed. This study was conducted at the Teaching and Research Farm of the University of Dschang to evaluate the Effect of <span style="white-space:nowrap;"><i>Cucurma longa</i></span> powder as feed additive on hemato-serological parameters in Guinea pigs. A total of 120 three weeks old Guinea pigs (60 males and 60 females), with an average weight of 150 ± 20 g were used. The animals were randomly distributed into four treatments in a completely randomized design with 30 animals per treatment (15 males and 15 females). Four experimental treatments were used by adding 0%, 0.25%, 0.5% and 1% <i>C. longa</i> in the diet corresponding to R0, R0.25%, R0.5% and R1% respectively. At the 8th week of age, 12 Guinea pigs (6 males and 6 females) per treatment were fasted for 12 hours and sacrificed by cervical dislocation. Blood was rapidly collected from the jugular vein for the evaluation of haematological and biochemical parameters. Results revealed that the inclusion of <span style="white-space:normal;"><i></span>Curcuma longa<span style="white-space:normal;"></i></span> at 0.25% significantly (p < 0.05) increased the concentration of hematocrit, lymphocytes, monocytes, granulocytes and platelets. A significant decrease (p < 0.05) was observed in hemoglobin, red blood cells, white blood cells, serum lipids, globulin, AST, ALT, and urea. It was concluded that the use of <span style="white-space:normal;"><i></span>Curcuma longa<span style="white-space:normal;"></i></span> powder at 0.25% as feed additive positively regulates blood serum parameters of Guinea pigs.展开更多
The present study investigated the effect of partial replacement of soybean meal(Glycine max) with canola meal(CM)(Brassica napus) on the growth performance,haematology,serum biochemistry and meat quality characterist...The present study investigated the effect of partial replacement of soybean meal(Glycine max) with canola meal(CM)(Brassica napus) on the growth performance,haematology,serum biochemistry and meat quality characteristics of female Japanese quails in a 35-day feeding trial.One hundred and forty6-week-old quails 158.28 ± 11.919 g were randomly allocated to 5 isonitrogenous and isoenergetic experimental diets: control diet(CM0; with no CM inclusion); CM0 with 2.5%(CM25),5.0%(CM50),12.5%(CM125) and 17.5%(CM175) soybean meal replaced with CM.Average weekly gain(AWG) and feed conversion efficiency(FCE) were determined.Haematology,serum biochemistry,carcass traits and meat quality parameters were determined at slaughter.Quails fed CM175 had the lowest(P < 0.05) feed intake whereas no differences were observed among the other 4 diets.No dietary effects on AWG,FCE and haematological parameters were observed.Serum biochemical parameters were not influenced by diets with the exception of alkaline phosphatase(ALP),where quails fed CM25 had higher ALP(161.0 U/L) than those fed CM0(37.25 U/L).Quails fed CM25 had the highest chroma(7.39) while those fed CM125 had the lowest(3.58) at 24 h post-slaughter.Diets had no influence(P > 0.05) on cooking losses and peak positive force of quail meat.It was concluded that CM can replace soybean in quail diets up to 12.5% without compromising growth performance,health and quality of meat.Inclusion levels beyond 12.5% promoted poor voluntary feed intake and thus may require feed additives to enhance utilization.展开更多
Objective:To explore cytotoxic activity of ethanol extract of Alpinia calcarata Rose(EEAC) rhizome against Ehiiich ascites carcinoma(EAC) tumor bearing Swiss Albino mice.Methods: In the present study,its anti-neoplast...Objective:To explore cytotoxic activity of ethanol extract of Alpinia calcarata Rose(EEAC) rhizome against Ehiiich ascites carcinoma(EAC) tumor bearing Swiss Albino mice.Methods: In the present study,its anti-neoplastic activity has been studied by monitoring parameters like tumor weight measurement,survival time,tumor cell growth inhibition,haematological characteristics etc.Results:It was found that EEAC at dose 8 mg/kg/day(i.p.) significantly decreased tumor weight(62.0%;P【0.01),increased life span(70.25%;P 【0.01) and reduced tumor cell growth rate(85.7%;P【0.01) in comparison to those of EAC bearing mice.The plant extract also improved the depleted haematological parameters like RBC WBC,Hb%,differential counts(e.g.lymphocytes,neutrophils,monocytes etc) of EAC bearing mice towards normal.The host toxic effects were not very high and recovered gradually towards normal within a few days after treatment.ConchiskHis:EEAC exhibits potent in vivo cytotoxic activity against EAC tumor bearing Swiss Albino mice.So,the plant can be considered as a probable new source of antitumor agents.展开更多
The blue fox (Vulpes lagopus) is a colour mutation of wild arctic fox. It is commonly farmed for its fur. The aim of the present study was to analyse a baseline biochemical profile of serum from adult farmed blue foxe...The blue fox (Vulpes lagopus) is a colour mutation of wild arctic fox. It is commonly farmed for its fur. The aim of the present study was to analyse a baseline biochemical profile of serum from adult farmed blue foxes during pelting season. Serum biochemical profile can be used later on as a reference in chemical analysis of blood serum. Biochemical profile here consists of liver enzymes alanine aminotransferase (ALAT), aspartatate aminotranasferase (ASAT), alkaline phospahatase (ALP), and total bilirubin (T-BIL);kidney function substances creatinine, urea and inorganic phosphate (Pi);muscle enzyme creatinine kinase (CK);glucose (GLUK) from pancreas;serum total proteins (PROT) and albumin (ALB);electrolytes potassium (K), natrium (Na), chloride (CL), calcium (S-Ca);and lipids such as total cholesterol (T-Cho). For some of the measured parameters reference values were also available from previous studies. Certain values were close to those previously published but some differed markedly. These differences can be explained by differences e.g. in techniques, conditions, unit reports, and breed and age of the animals. The present study additionally reviewed previously published haematological data concerning small blood count, i.e. hemoglobin, hematocrit, white blood cells and red blood cells. Summarized material revealed that these variables are very similar despite of study and animal material. Thus, small blood count can be reliably used to screen general welfare status of farmed blue foxes. The reference values presented in this paper will be valuable as a guide for clinical diagnosis and research.展开更多
<span style="font-family:Verdana;">An experiment was conducted to evaluate the performance and blood para</span><span style="font-family:Verdana;">meters</span><span styl...<span style="font-family:Verdana;">An experiment was conducted to evaluate the performance and blood para</span><span style="font-family:Verdana;">meters</span><span style="font-family:Verdana;"> of West African Dwarf (WAD) bucks fed graded levels of toasted</span><span style="font-family:Verdana;"> baobab seed meal for 84 days. Baobab seeds were washed, sun-dried and toasted uniformly using a cast iron pan. The toasted baobab seeds were crushed with a roller mill grinding machine to make baobab seed meal (BSM). The BSM </span><span style="font-family:Verdana;">was included at 0%, 25%, 50% and 75% levels in diets 1, 2, 3 and 4 </span><span style="font-family:Verdana;">respectively to replace soybean. Each treatment was replicated four times in a completely randomized design. Uncoagulated blood samples were collected from WAD bucks at the end of the 84 days</span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">’</span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;"> feeding trial and analysed for packed cell volume (PCV), haemoglobin concentrate (Hb), red blood cells (RBC) and w</span><span style="font-family:Verdana;">hite blood cells (WBC). The mean corpuscular haemoglobin volu</span><span style="font-family:Verdana;">me (MCV), mean corpuscular haemoglobin (MCH), platelets, neutrophils, lymphocytes, monocytes, eosinophils, and basophils were calculated using PCV, RBC and Hb. The blood meant for serological analysis was centrifuged at 1000</span></span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">G for 10 minutes, after which the serum was separated and used for determining serum total protein (Tp), albumin, serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT).</span></span></span><span><span><span style="font-family:;" "=""> <span展开更多
Background: Measurement of haematological parameters has been historically helpful in the diagnosis of many diseases in endurance sportsmen. The modifications of these parameters during endurance race have not yet bee...Background: Measurement of haematological parameters has been historically helpful in the diagnosis of many diseases in endurance sportsmen. The modifications of these parameters during endurance race have not yet been evaluated in many African countries. Objectives: To determine haematological values before and immediately after a half-marathon event, as well as within 24 hours after the race and to analyze the changes observed. Methods: A cross-sectional study was conducted from 10 to 21 August 2018 at Brazzaville, Congo. All measurements were confined to 76 male participants (39 specialists vs 37 no specialists of endurance race) in the Brazzaville half-marathon (21.1 km), aged between 19 - 39 years (mean age: 26.7 ± 2.6 years). Coulter profiles with differential white cell counts and haptoglobin levels were determined in venous sample before and after competitive half-marathon race. The same measurements were performed during the 24 hrs following the competition. Results: In the pre-race sample, mild anemia was detected in 12 subjects and mild thrombocytopenia in 7 subjects. Haptoglobin levels were reduced in 5 subjects. Haematological values, all post-race, varied significantly before and after race, particularly for RBC, Hb, Hct, PLT, MCV, MCH, MCHC, WBC, neutrophil counts, lymphocyte counts, monocyte counts, basophil counts, eosinophil counts and haptoglobin. These differences between specialists and no specialists were statistically (p Conclusion: Our data may help sport physicians, sport physiologists and trainers to better follow-up haematological reactions associated with the half-marathon race.展开更多
Bt corn “MON810: Ajeeb YG” produces delta endotoxins in the whole plant due to the genetic modification process. The chemical analysis of this variety showed significant differences from its conventional counterpart...Bt corn “MON810: Ajeeb YG” produces delta endotoxins in the whole plant due to the genetic modification process. The chemical analysis of this variety showed significant differences from its conventional counterpart “Ajeeb”. Further, feeding studies on rats were designed to complete assessing the safety of “MON810: Ajeeb YG”. Three groups of rats (6 males and 6 females’ rats/group) were fed on control diet, non-Bt corn and Bt corn for 1.5 months and 3 months. After 1.5 months, 3 males and 3 females from each group were sacrificed, and after another 1.5 months, 3 males and 3 females from each group and their offspring were sacrificed. Histopathological examination, blood haematology and serum biochemical analysis were determined. Results indicated significant differences among the tested parameters in the three groups especially Bt group. Severe changes in the liver of Bt group after 3 months were observed.展开更多
文摘Objective:To investigate the antidiabetic properties of aqueous extract of stem bark of Afzelia africana(A.africana)and its beneficial effect on haematological parameters in streptozotocin induced diabetic rats.Methods:A total of 30 rats including 24 diabetic and 6 normal rats were used for this study.Diabetes was induced in male Wistar rats by intraperitoneal injection of streptozotocin.After being confirmed diabetic,animals were orally treated with distilled water or extracts at 100 or 200 mg/kg body weight daily for 10 days.The haematological parameters including red blood and white blood cells and their functional indices were evaluated in diabetic treated groups compared with the controls.Results:The extract significantly reduced the blood glucose levels while the best result was obtained at 200 mg/kg body weight The feed and water intake in diabetic rats were significantly reduced while weight loss was minimized at both dosages.Similarly,the levels of red blood,white blood cells and their functional indices were significantly improved after extract administration at both doses.Conclusions:It can be concluded that the aqueous extract of bark of A.africana possesses antihyperglycemic properties.In addition,the extract can prevent various complications of diabetes and improve some haematological parameters.Further experimental investigation is needed to exploit its relevant therapeutic effect to substantiate its ethnomedicinal usage.
文摘Background: Adequate selection of a prospective whole blood donor protects his health and safety of the recipient. Objectives: The main objective of this study was to determine the haematology parameters of apparently healthy prospective whole blood donors. Participants and Methods: This was a hospital based prospective study carried out from August to October 2020 at the blood transfusion unit of the Lagos State University Teaching Hospital (LASUTH), Ikeja, Nigeria. A structured pretested questionnaire was used for data collection. The socio demographic status and the haematology parameters of apparently healthy prospective whole blood donors who tested negative for HIV, hepatitis B and C markers were captured. Obtained data were analysed with the statistical package for the social scientist software version 20. Results: One hundred male (97.1%) and three female (2.9%) apparently healthy prospective whole blood donors were studied. The median age of study subjects was 30 years. Obtained median haematology parameter values were 13 g/dl, 40%, 4.9/nl and 203.9/nl for haemoglobin concentration, haematocrit, total white cell and platelet counts respectively. The median values for the mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) and mean corpuscular volume (MCV) of participants were 32.6 g/dl, 27.7 pg and 85.7 fl respectively. Observed prevalence of subnormal haematology parameters for haemoglobin concentration, total white cells, platelets were 12.6%, 25.2%, and 13.6% respectively. Also subnormal values for MCHC, MCH, MCV were 11.7%, 26.2%, and 16.5% respectively among prospective whole blood donors in this study. No higher than normal haematology parameter values were observed. Median values for erythrocyte sedimentation rate was 8.4 mm/hr. Conclusion: A significant percentage of apparently healthy prospective whole blood donors had subnormal haematology parameters values. Obtained normal values in our study are comparable with local reference range reports from previous stud
基金Supported by Directorate of Research,Guru Angad Dev Veterinary and Animal Sciences University,Ludhiana-141004,India.Grant No.-RKVYB-2(Ⅲ)c(d)DR-2,sanctioned on October,2010
文摘Objective:To evaluate clinical and hematological aspects of dogs naturally infected with Hepatozoon canis(H.canis)presented at the Small Animal Clinics of Guru Angad Dev Veterinary and Animal Sciences University,Ludhiana.Methods:Blood films of 34 naturally infected dogs were examined for haematological alterations and parasitaemia.Signalment and clinical signs were recorded from the animals.Clinical histories were filled oul during the consultation.Results:Of the 34 positive dogs by Giemsa stained peripheral blood films.88.23%presented parasilaemia by H.canis only,while 11.77%had the combination of H.canis,Babesia sp.and Ehrlichia sp.Young male dogs less than oue-year-old,of nondescript breed,were the most commonly affected.And 26.47%were presented with anorexia/inappetence as the only clinical symptom.Other clinical symptoms were mild to moderate fever,pale mucosae and lethargy;a few were also showing the signs of vomiting and diarrhoea.Haematological alterations showed mainly normochromic-normocytic anaemia,leukocytosis and neutrophilia.Conclusions:The findings of this study substantiate that H.canis caused clinical and haematological alterations of the varied intensity in dogs,even with low parasilaemia.should he taken into consideration.
文摘Objective:Voriconazole(VCZ)is a triazole antifungal agent widely used in immunocompromised patients with suspected or proven invasive fungal infections.The achievement of therapeutic range(1-5 mg/L)is essential to maximize VCZ efficacy,as its pharmacokinetics is characterized by a wide inter-and intra-individual variability.This study aims to quantify the variability of VCZ trough concentrations in children and adolescents with haematological diseases and optimize therapeutic drug monitoring in clinical practice.Methods:We analysed the monitoring concentrations of all children(<18 years old)treated with VCZ in the Haematology Department of Robert DebréHospital between January 2014 and December 2016.Demographic,clinical data,and VCZ dosing and monitoring concentrations measured by high-performance liquid chromatography with ultraviolet detection(HPLC-UV)were analysed.Non-parametric tests were performed using SPSS IBM 24.0.Results:380 trough VCZ concentrations at steady-state(Ctrough,ss)were available in 79 children:45.6%had first Ctrough,ss in the therapeutic range at first monitoring,46.8%had Ctrough,ss below 1 mg/L and 7.6%had Ctrough,ss over 5 mg/L.Forty-one patients were treated with recommended doses but only 53%of them reached the therapeutic range.There was no impact of age,sex,biological parameters,or indication of VCZ on Ctrough,ss values.The number of Ctrough,ss in the therapeutic range increases with the number of monitoring per patient following dosage adaptations.Conclusion:The wide inter-and intra-individual variability of VCZ trough concentrations at recommended doses confirm the need to standardize VCZ monitoring and identify factors to be considered to prospectively adapt treatment for each patient.
文摘The reduced efficiency of antibiotic growth promoters in livestock, and the unavoidable residual effects on livestock products such as egg, meat and milk have pushed researchers to rush towards the use of phytobiotics in animal feed. This study was conducted at the Teaching and Research Farm of the University of Dschang to evaluate the Effect of <span style="white-space:nowrap;"><i>Cucurma longa</i></span> powder as feed additive on hemato-serological parameters in Guinea pigs. A total of 120 three weeks old Guinea pigs (60 males and 60 females), with an average weight of 150 ± 20 g were used. The animals were randomly distributed into four treatments in a completely randomized design with 30 animals per treatment (15 males and 15 females). Four experimental treatments were used by adding 0%, 0.25%, 0.5% and 1% <i>C. longa</i> in the diet corresponding to R0, R0.25%, R0.5% and R1% respectively. At the 8th week of age, 12 Guinea pigs (6 males and 6 females) per treatment were fasted for 12 hours and sacrificed by cervical dislocation. Blood was rapidly collected from the jugular vein for the evaluation of haematological and biochemical parameters. Results revealed that the inclusion of <span style="white-space:normal;"><i></span>Curcuma longa<span style="white-space:normal;"></i></span> at 0.25% significantly (p < 0.05) increased the concentration of hematocrit, lymphocytes, monocytes, granulocytes and platelets. A significant decrease (p < 0.05) was observed in hemoglobin, red blood cells, white blood cells, serum lipids, globulin, AST, ALT, and urea. It was concluded that the use of <span style="white-space:normal;"><i></span>Curcuma longa<span style="white-space:normal;"></i></span> powder at 0.25% as feed additive positively regulates blood serum parameters of Guinea pigs.
基金financial support from Health and Welfare Sector Education and Training Authority towards this projectthe funding received from the North-West University Ph D bursary
文摘The present study investigated the effect of partial replacement of soybean meal(Glycine max) with canola meal(CM)(Brassica napus) on the growth performance,haematology,serum biochemistry and meat quality characteristics of female Japanese quails in a 35-day feeding trial.One hundred and forty6-week-old quails 158.28 ± 11.919 g were randomly allocated to 5 isonitrogenous and isoenergetic experimental diets: control diet(CM0; with no CM inclusion); CM0 with 2.5%(CM25),5.0%(CM50),12.5%(CM125) and 17.5%(CM175) soybean meal replaced with CM.Average weekly gain(AWG) and feed conversion efficiency(FCE) were determined.Haematology,serum biochemistry,carcass traits and meat quality parameters were determined at slaughter.Quails fed CM175 had the lowest(P < 0.05) feed intake whereas no differences were observed among the other 4 diets.No dietary effects on AWG,FCE and haematological parameters were observed.Serum biochemical parameters were not influenced by diets with the exception of alkaline phosphatase(ALP),where quails fed CM25 had higher ALP(161.0 U/L) than those fed CM0(37.25 U/L).Quails fed CM25 had the highest chroma(7.39) while those fed CM125 had the lowest(3.58) at 24 h post-slaughter.Diets had no influence(P > 0.05) on cooking losses and peak positive force of quail meat.It was concluded that CM can replace soybean in quail diets up to 12.5% without compromising growth performance,health and quality of meat.Inclusion levels beyond 12.5% promoted poor voluntary feed intake and thus may require feed additives to enhance utilization.
文摘Objective:To explore cytotoxic activity of ethanol extract of Alpinia calcarata Rose(EEAC) rhizome against Ehiiich ascites carcinoma(EAC) tumor bearing Swiss Albino mice.Methods: In the present study,its anti-neoplastic activity has been studied by monitoring parameters like tumor weight measurement,survival time,tumor cell growth inhibition,haematological characteristics etc.Results:It was found that EEAC at dose 8 mg/kg/day(i.p.) significantly decreased tumor weight(62.0%;P【0.01),increased life span(70.25%;P 【0.01) and reduced tumor cell growth rate(85.7%;P【0.01) in comparison to those of EAC bearing mice.The plant extract also improved the depleted haematological parameters like RBC WBC,Hb%,differential counts(e.g.lymphocytes,neutrophils,monocytes etc) of EAC bearing mice towards normal.The host toxic effects were not very high and recovered gradually towards normal within a few days after treatment.ConchiskHis:EEAC exhibits potent in vivo cytotoxic activity against EAC tumor bearing Swiss Albino mice.So,the plant can be considered as a probable new source of antitumor agents.
文摘The blue fox (Vulpes lagopus) is a colour mutation of wild arctic fox. It is commonly farmed for its fur. The aim of the present study was to analyse a baseline biochemical profile of serum from adult farmed blue foxes during pelting season. Serum biochemical profile can be used later on as a reference in chemical analysis of blood serum. Biochemical profile here consists of liver enzymes alanine aminotransferase (ALAT), aspartatate aminotranasferase (ASAT), alkaline phospahatase (ALP), and total bilirubin (T-BIL);kidney function substances creatinine, urea and inorganic phosphate (Pi);muscle enzyme creatinine kinase (CK);glucose (GLUK) from pancreas;serum total proteins (PROT) and albumin (ALB);electrolytes potassium (K), natrium (Na), chloride (CL), calcium (S-Ca);and lipids such as total cholesterol (T-Cho). For some of the measured parameters reference values were also available from previous studies. Certain values were close to those previously published but some differed markedly. These differences can be explained by differences e.g. in techniques, conditions, unit reports, and breed and age of the animals. The present study additionally reviewed previously published haematological data concerning small blood count, i.e. hemoglobin, hematocrit, white blood cells and red blood cells. Summarized material revealed that these variables are very similar despite of study and animal material. Thus, small blood count can be reliably used to screen general welfare status of farmed blue foxes. The reference values presented in this paper will be valuable as a guide for clinical diagnosis and research.
文摘<span style="font-family:Verdana;">An experiment was conducted to evaluate the performance and blood para</span><span style="font-family:Verdana;">meters</span><span style="font-family:Verdana;"> of West African Dwarf (WAD) bucks fed graded levels of toasted</span><span style="font-family:Verdana;"> baobab seed meal for 84 days. Baobab seeds were washed, sun-dried and toasted uniformly using a cast iron pan. The toasted baobab seeds were crushed with a roller mill grinding machine to make baobab seed meal (BSM). The BSM </span><span style="font-family:Verdana;">was included at 0%, 25%, 50% and 75% levels in diets 1, 2, 3 and 4 </span><span style="font-family:Verdana;">respectively to replace soybean. Each treatment was replicated four times in a completely randomized design. Uncoagulated blood samples were collected from WAD bucks at the end of the 84 days</span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">’</span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;"> feeding trial and analysed for packed cell volume (PCV), haemoglobin concentrate (Hb), red blood cells (RBC) and w</span><span style="font-family:Verdana;">hite blood cells (WBC). The mean corpuscular haemoglobin volu</span><span style="font-family:Verdana;">me (MCV), mean corpuscular haemoglobin (MCH), platelets, neutrophils, lymphocytes, monocytes, eosinophils, and basophils were calculated using PCV, RBC and Hb. The blood meant for serological analysis was centrifuged at 1000</span></span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">G for 10 minutes, after which the serum was separated and used for determining serum total protein (Tp), albumin, serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT).</span></span></span><span><span><span style="font-family:;" "=""> <span
文摘Background: Measurement of haematological parameters has been historically helpful in the diagnosis of many diseases in endurance sportsmen. The modifications of these parameters during endurance race have not yet been evaluated in many African countries. Objectives: To determine haematological values before and immediately after a half-marathon event, as well as within 24 hours after the race and to analyze the changes observed. Methods: A cross-sectional study was conducted from 10 to 21 August 2018 at Brazzaville, Congo. All measurements were confined to 76 male participants (39 specialists vs 37 no specialists of endurance race) in the Brazzaville half-marathon (21.1 km), aged between 19 - 39 years (mean age: 26.7 ± 2.6 years). Coulter profiles with differential white cell counts and haptoglobin levels were determined in venous sample before and after competitive half-marathon race. The same measurements were performed during the 24 hrs following the competition. Results: In the pre-race sample, mild anemia was detected in 12 subjects and mild thrombocytopenia in 7 subjects. Haptoglobin levels were reduced in 5 subjects. Haematological values, all post-race, varied significantly before and after race, particularly for RBC, Hb, Hct, PLT, MCV, MCH, MCHC, WBC, neutrophil counts, lymphocyte counts, monocyte counts, basophil counts, eosinophil counts and haptoglobin. These differences between specialists and no specialists were statistically (p Conclusion: Our data may help sport physicians, sport physiologists and trainers to better follow-up haematological reactions associated with the half-marathon race.
文摘Bt corn “MON810: Ajeeb YG” produces delta endotoxins in the whole plant due to the genetic modification process. The chemical analysis of this variety showed significant differences from its conventional counterpart “Ajeeb”. Further, feeding studies on rats were designed to complete assessing the safety of “MON810: Ajeeb YG”. Three groups of rats (6 males and 6 females’ rats/group) were fed on control diet, non-Bt corn and Bt corn for 1.5 months and 3 months. After 1.5 months, 3 males and 3 females from each group were sacrificed, and after another 1.5 months, 3 males and 3 females from each group and their offspring were sacrificed. Histopathological examination, blood haematology and serum biochemical analysis were determined. Results indicated significant differences among the tested parameters in the three groups especially Bt group. Severe changes in the liver of Bt group after 3 months were observed.
