Decades have passed since the first discovery of H10-subtype avian influenza virus(AIV) in chickens in 1949,and it has been detected in many species including mammals such as minks,pigs,seals and humans.Cases of human...Decades have passed since the first discovery of H10-subtype avian influenza virus(AIV) in chickens in 1949,and it has been detected in many species including mammals such as minks,pigs,seals and humans.Cases of human infections with H10N8viruses identified in China in 2013 have raised widespread attention.Two novel reassortant H10N3 viruses were isolated from chickens in December 2019 in eastern China during routine surveillance for AIVs.The internal genes of these viruses were derived from genotype S(G57) H9N2 and were consistent with H5N6,H7N9 and H10N8,which cause fatal infections in humans.Their viral pathogenicity and transmissibility were further studied in different animal models.The two H10N3 isolates had low pathogenicity in chickens and were transmitted between chickens via direct contact.These viruses were highly pathogenic in mice and could be transmitted between guinea pigs via direct contact and respiratory droplets.More importantly,these viruses can bind to both human-type SAα-2,6-Gal receptors and avian-type SAα-2,3-Gal receptors.Asymptomatic shedding in chickens and good adaptability to mammals of these H10N3 isolates would make it easier to transmit to humans and pose a threat to public health.展开更多
The H10 subtype avian influenza virus(AIV)poses an ongoing threat to both birds and humans.Notably,fatal human cases of H10N3 and H10N8 infections have drawn public attention.In 2022,we isolated two H10N3 viruses(A/ch...The H10 subtype avian influenza virus(AIV)poses an ongoing threat to both birds and humans.Notably,fatal human cases of H10N3 and H10N8 infections have drawn public attention.In 2022,we isolated two H10N3 viruses(A/chicken/Shandong/0101/2022 and A/chicken/Shandong/0603/2022)from diseased chickens in China.Genome analysis revealed that these viruses were genetically associated with human-origin H10N3 virus,with internal genes originating from local H9N2 viruses.Compared to the H10N8 virus(A/chicken/Jiangxi/102/2013),the H10N3 viruses exhibited enhanced thermostability,increased viral release from erythrocytes,and accumulation of hemagglutinin(HA)protein.Additionally,we evaluated the pathogenicity of both H10N3 and H10N8 viruses in mice.We found that viral titers could be detected in the lungs and nasal turbinates of mice infected with the two H10N3 viruses,whereas H10N8 virus titers were detectable in the lungs and brains of mice.Notably,the proportion of double HA Q222R and G228S mutations in H10N3 viruses has increased since 2019.However,the functional roles of the Q222R and G228S double mutations in the HA gene of H10N3 viruses remain unknown and warrant further investigation.Our study highlights the potential public health risk posed by the H10N3 virus.A spillover event of AIV to humans could be a foretaste of a looming pandemic.Therefore,it is imperative to continuously monitor the evolution of the H10N3 influenza virus to ensure targeted prevention and control measures against influenza outbreaks.展开更多
基金supported by the National Key Research and Development Project of China (2016YFD0500202-1,2016YFD0501601)the National Natural Science Foundation of China(31772755)+3 种基金Jiangsu Provincial Natural Science Fund for Excellent Young Scholars (BK20170068)the Earmarked Fund For China Agriculture Research System (CARS-40)the Open Project Program of Jiangsu Key Laboratory of Zoonosis (R1808)the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)。
文摘Decades have passed since the first discovery of H10-subtype avian influenza virus(AIV) in chickens in 1949,and it has been detected in many species including mammals such as minks,pigs,seals and humans.Cases of human infections with H10N8viruses identified in China in 2013 have raised widespread attention.Two novel reassortant H10N3 viruses were isolated from chickens in December 2019 in eastern China during routine surveillance for AIVs.The internal genes of these viruses were derived from genotype S(G57) H9N2 and were consistent with H5N6,H7N9 and H10N8,which cause fatal infections in humans.Their viral pathogenicity and transmissibility were further studied in different animal models.The two H10N3 isolates had low pathogenicity in chickens and were transmitted between chickens via direct contact.These viruses were highly pathogenic in mice and could be transmitted between guinea pigs via direct contact and respiratory droplets.More importantly,these viruses can bind to both human-type SAα-2,6-Gal receptors and avian-type SAα-2,3-Gal receptors.Asymptomatic shedding in chickens and good adaptability to mammals of these H10N3 isolates would make it easier to transmit to humans and pose a threat to public health.
基金supported by the National Natural Science Foundation of China(32330104,32302956)the China Postdoctoral Science Foundation(2023T160252,Jiahao Zhang)+1 种基金the Guangzhou Science and Technology Plan Project(SL2022B03J01423)the Changjiang Distinguished Professor Program(2023,Wenbao Qi).
文摘The H10 subtype avian influenza virus(AIV)poses an ongoing threat to both birds and humans.Notably,fatal human cases of H10N3 and H10N8 infections have drawn public attention.In 2022,we isolated two H10N3 viruses(A/chicken/Shandong/0101/2022 and A/chicken/Shandong/0603/2022)from diseased chickens in China.Genome analysis revealed that these viruses were genetically associated with human-origin H10N3 virus,with internal genes originating from local H9N2 viruses.Compared to the H10N8 virus(A/chicken/Jiangxi/102/2013),the H10N3 viruses exhibited enhanced thermostability,increased viral release from erythrocytes,and accumulation of hemagglutinin(HA)protein.Additionally,we evaluated the pathogenicity of both H10N3 and H10N8 viruses in mice.We found that viral titers could be detected in the lungs and nasal turbinates of mice infected with the two H10N3 viruses,whereas H10N8 virus titers were detectable in the lungs and brains of mice.Notably,the proportion of double HA Q222R and G228S mutations in H10N3 viruses has increased since 2019.However,the functional roles of the Q222R and G228S double mutations in the HA gene of H10N3 viruses remain unknown and warrant further investigation.Our study highlights the potential public health risk posed by the H10N3 virus.A spillover event of AIV to humans could be a foretaste of a looming pandemic.Therefore,it is imperative to continuously monitor the evolution of the H10N3 influenza virus to ensure targeted prevention and control measures against influenza outbreaks.
基金国家重点研发计划(项目号:2022YFC2601602),题目:重要跨境野生动物病原传播机制及发生风险中美疾控中心合作项目(项目号:5 U01IP001106⁃04⁃00),题目:China⁃U.S.Collaborative Program on Emerging and Re⁃emerging Infectious Diseases+3 种基金广州国家实验室自立项目(项目号:SRPG22⁃001),题目:重要呼吸道病原遗传变异特征和跨种传播机制研究中国科学院国际合作局—海教中心(项目号:151C53KYSB20210023),题目:禽流感病毒的遗传多样性与流行风险预警中国科学院青年创新促进会(项目号:Y2021034)泰山学者青年专家计划项目(项目号:tsqn202211217)。
