Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats.However,the underlying mechanisms are poorly understood.In this study,we used the rat middle cerebral artery occlusion model...Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats.However,the underlying mechanisms are poorly understood.In this study,we used the rat middle cerebral artery occlusion model of stroke.Three days after surgery,absorbable surgical catgut sutures were implanted at Dazhui（GV14）,Jizhong（GV6）,Houhui,Guanyuan（CV4）and Zhongwan（CV12）.The Zea Longa score was used to assess neurological function.The Modified Ashworth Scale was used to evaluate muscle tension.The 2,3,5-triphenyl-tetrazolium chloride assay was used to measure infarct volume.Immunohistochemical staining was performed for glutamate aspartate transporter（GLAST）and glial glutamate transporter-1（GLT-1）expression.Western blot assay was used to analyze the expression of GLAST and GLT-1.Reverse transcription and polymerase chain reaction were carried out to assess the expression of GLAST and GLT-1m RNAs.After catgut implantation at the acupoints,neurological function was substantially improved,muscle tension was decreased,and infarct volume was reduced in rats with spasticity after stroke.Furthermore,the expression of GLAST and GLT-1 m RNAs was increased on the injured（left）side.Our findings demonstrate that catgut implantation at acupoints alleviates spasticity after stroke,likely by increasing the expression of GLAST and GLT-1.展开更多
Objective： To observe the effects of moxibustion at different times on prostaglandin E_2（PGE_2）, prostaglandin F2α（PGF_（2α））and arginine vasopressin（AVP）, in the uterine tissues of rats with dysmenorrhea du...Objective： To observe the effects of moxibustion at different times on prostaglandin E_2（PGE_2）, prostaglandin F2α（PGF_（2α））and arginine vasopressin（AVP）, in the uterine tissues of rats with dysmenorrhea due to cold-dampness retention, and to explore the differences and possible mechanisms of moxibustion at different times in easing pain in dysmenorrhea due to cold-dampness retention.Methods： Forty-three female Wistar rats were randomly divided into a blank control group（n=7）, a model group（n=9）,a pre-moxibustion group（n=9）, an immediate-moxibustion group（n=9） and a pre-moxibustion plus immediatemoxibustion group（n=9）. Rat models of primary dysmenorrhea due to cold-dampness retention were established using（0±1） ℃ ice water immersion method combined with injection of estradiol benzoate for 10 d, followed by injection of oxytocin on the 11 th day. Rats in the 3 intervention groups received moxibustion to Shenque（CV 8） and Guanyuan（CV 4）, 10 min for each acupoint, once a day. Rats in pre-moxibustion group were given mild moxibustion, beginning on the 8th day during modeling, for 3 continuous days; rats in immediate-moxibustion group were given one time mild moxibustion, immediately after injection of oxytocin on the 11 th day during modeling; rats in pre-moxibustion plus immediate-moxibustion group were given mild moxibustion, beginning on the 8th day during modeling till immediately after injection of oxytocin on the 11 th day during modeling, for 4 continuous days. The level of PGF_（2α） in the rat uterine tissues was measured by enzyme-linked immunosorbent assay（ELISA）, and the levels of PGE_2 and AVP in rat uterine tissues were measured by radioimmunoassay.Results： Compared with the blank control group, the levels of PGF_（2α） and AVP, the PGF_（2α）/PGE_2 ratio in the model group were significantly increased（P〈0.01）, and the PGE_2 level was significantly decreased（P〈0.01） in the rat uterine tissues in the model group. Compared展开更多
基金supported by the National Natural Science Foundation of China,No.14202225,&.81574042the Traditional Chinese Medicine Leading Talent Funding Projects of Henan Province of China,No.2000202a grant from the Special Research Project on the Construction of the National Traditional Chinese Medicine Clinical Research Base of the State Administration of Traditional Chinese Medicine of China,No.JDZX2015314
文摘Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats.However,the underlying mechanisms are poorly understood.In this study,we used the rat middle cerebral artery occlusion model of stroke.Three days after surgery,absorbable surgical catgut sutures were implanted at Dazhui（GV14）,Jizhong（GV6）,Houhui,Guanyuan（CV4）and Zhongwan（CV12）.The Zea Longa score was used to assess neurological function.The Modified Ashworth Scale was used to evaluate muscle tension.The 2,3,5-triphenyl-tetrazolium chloride assay was used to measure infarct volume.Immunohistochemical staining was performed for glutamate aspartate transporter（GLAST）and glial glutamate transporter-1（GLT-1）expression.Western blot assay was used to analyze the expression of GLAST and GLT-1.Reverse transcription and polymerase chain reaction were carried out to assess the expression of GLAST and GLT-1m RNAs.After catgut implantation at the acupoints,neurological function was substantially improved,muscle tension was decreased,and infarct volume was reduced in rats with spasticity after stroke.Furthermore,the expression of GLAST and GLT-1 m RNAs was increased on the injured（left）side.Our findings demonstrate that catgut implantation at acupoints alleviates spasticity after stroke,likely by increasing the expression of GLAST and GLT-1.
基金supported by Science and Technology Support Project of Hebei Provincial Administration of Traditional Chinese Medicine,No.2015089Science and Technology Research Project of Universities in Hebei Province,No.QN2015027Undergraduate Student Innovation Project of Hebei Province Education Office,No.201514432017~~
文摘Objective： To observe the effects of moxibustion at different times on prostaglandin E_2（PGE_2）, prostaglandin F2α（PGF_（2α））and arginine vasopressin（AVP）, in the uterine tissues of rats with dysmenorrhea due to cold-dampness retention, and to explore the differences and possible mechanisms of moxibustion at different times in easing pain in dysmenorrhea due to cold-dampness retention.Methods： Forty-three female Wistar rats were randomly divided into a blank control group（n=7）, a model group（n=9）,a pre-moxibustion group（n=9）, an immediate-moxibustion group（n=9） and a pre-moxibustion plus immediatemoxibustion group（n=9）. Rat models of primary dysmenorrhea due to cold-dampness retention were established using（0±1） ℃ ice water immersion method combined with injection of estradiol benzoate for 10 d, followed by injection of oxytocin on the 11 th day. Rats in the 3 intervention groups received moxibustion to Shenque（CV 8） and Guanyuan（CV 4）, 10 min for each acupoint, once a day. Rats in pre-moxibustion group were given mild moxibustion, beginning on the 8th day during modeling, for 3 continuous days; rats in immediate-moxibustion group were given one time mild moxibustion, immediately after injection of oxytocin on the 11 th day during modeling; rats in pre-moxibustion plus immediate-moxibustion group were given mild moxibustion, beginning on the 8th day during modeling till immediately after injection of oxytocin on the 11 th day during modeling, for 4 continuous days. The level of PGF_（2α） in the rat uterine tissues was measured by enzyme-linked immunosorbent assay（ELISA）, and the levels of PGE_2 and AVP in rat uterine tissues were measured by radioimmunoassay.Results： Compared with the blank control group, the levels of PGF_（2α） and AVP, the PGF_（2α）/PGE_2 ratio in the model group were significantly increased（P〈0.01）, and the PGE_2 level was significantly decreased（P〈0.01） in the rat uterine tissues in the model group. Compared
