The complete amino acid sequence of human augmenter of liver regeneration (hALR) was reported by deduction from nucleotide sequence of its complementary DNA . The cDNA for hALR was isolated by screening a human fetal ...The complete amino acid sequence of human augmenter of liver regeneration (hALR) was reported by deduction from nucleotide sequence of its complementary DNA . The cDNA for hALR was isolated by screening a human fetal liver cDNA library and the sequencing of this insert revealed an open reading frame encoding a protein with 125aa and highly homologous (87% ) with rat ALR encoding sequence. The recombinant hALR expressed from its cDNA in transient expression experiments in cos-7 cells could stimulate DNA synthesis of HTC hepatoma cell in the dose-dependent and heat-resistant way. Northern blot analysis with rat ALR cDNA as probe confirmed that ALR mRNA was expressed in the normal rat liver at low level and that dramatically increased in the regenerating liver after partial hepatectomied rat. This size of hALR mRNA is 1.4 kb long and expressed in human fetal liver, kidney and testis. These findings indicated that liver itself may be the resource of ALR and suggested that ALR seems to be an im-portant paracrined regulator of liver regeneration.展开更多
本实验结果表明,授粉三天后苹果花托中的生长促进物质活性比未授粉的增加10倍多,而生长抑制物水平明显减低;授粉七天后的花托继续保持较高的生长促进物活性和低的抑制物水平,但未授粉的花托中抑制物质水平显著增加,促进物质活性几乎全...本实验结果表明,授粉三天后苹果花托中的生长促进物质活性比未授粉的增加10倍多,而生长抑制物水平明显减低;授粉七天后的花托继续保持较高的生长促进物活性和低的抑制物水平,但未授粉的花托中抑制物质水平显著增加,促进物质活性几乎全部消失.用苹果花托中提取的类 GA 物质处理未授粉茄子子房,同 GA 一样明显促进子房的发育;而提取的生长抑制物处理已授粉的茄子,子房基本上停止了生长,据此讨论了生长调节物质在子房发育中的调控作用.展开更多
文摘The complete amino acid sequence of human augmenter of liver regeneration (hALR) was reported by deduction from nucleotide sequence of its complementary DNA . The cDNA for hALR was isolated by screening a human fetal liver cDNA library and the sequencing of this insert revealed an open reading frame encoding a protein with 125aa and highly homologous (87% ) with rat ALR encoding sequence. The recombinant hALR expressed from its cDNA in transient expression experiments in cos-7 cells could stimulate DNA synthesis of HTC hepatoma cell in the dose-dependent and heat-resistant way. Northern blot analysis with rat ALR cDNA as probe confirmed that ALR mRNA was expressed in the normal rat liver at low level and that dramatically increased in the regenerating liver after partial hepatectomied rat. This size of hALR mRNA is 1.4 kb long and expressed in human fetal liver, kidney and testis. These findings indicated that liver itself may be the resource of ALR and suggested that ALR seems to be an im-portant paracrined regulator of liver regeneration.
文摘本实验结果表明,授粉三天后苹果花托中的生长促进物质活性比未授粉的增加10倍多,而生长抑制物水平明显减低;授粉七天后的花托继续保持较高的生长促进物活性和低的抑制物水平,但未授粉的花托中抑制物质水平显著增加,促进物质活性几乎全部消失.用苹果花托中提取的类 GA 物质处理未授粉茄子子房,同 GA 一样明显促进子房的发育;而提取的生长抑制物处理已授粉的茄子,子房基本上停止了生长,据此讨论了生长调节物质在子房发育中的调控作用.
