The present study was designed to evaluate the immune-modulating effects of the polysaccharide from Grifola frondosa(GFP) by using mouse peritoneal macrophage and cytoxan(CTX) induced immunosuppression models. Our res...The present study was designed to evaluate the immune-modulating effects of the polysaccharide from Grifola frondosa(GFP) by using mouse peritoneal macrophage and cytoxan(CTX) induced immunosuppression models. Our results from the phagocytotic and mononuclear phagocytic system function assays showed that GFP-A(one component from GFP) stimulated the phagocytosis of the phagocytes. The splenocyte proliferation assay showed that GFP-A acted the effect combing Con A or LPS in splenocyte proliferation. The results showed that GFP-A increased indices of thymus and spleen, the levels of LDH and ACP in the spleen, the m RNA levels of IL-1β, IL-2, IL-6 and IFN-γ in splenocyte. And GFP-A also significantly increased the expression of CD4^+ and CD8^+ splenic T lymphocytes, which were suppressed by the CTX in peripheral blood. In conclusion, our results indicate that the GFP-A is involved in immunomodulatory effects leading to its modulatory effects on immunosuppression.展开更多
利用超声破碎、水提醇沉、葡聚糖凝胶过滤层析等方法,对灰树花子实体多糖进行分离得到多糖组分GFD-1。高效液相色谱分析表明,GFD-1是分子量为29.574 k Da的多糖均一组分。利用显色试验、紫外光谱、红外光谱及原子力显微镜分析,GFD-1为...利用超声破碎、水提醇沉、葡聚糖凝胶过滤层析等方法,对灰树花子实体多糖进行分离得到多糖组分GFD-1。高效液相色谱分析表明,GFD-1是分子量为29.574 k Da的多糖均一组分。利用显色试验、紫外光谱、红外光谱及原子力显微镜分析,GFD-1为不含还原性羰基、酚羟基、游离或结合蛋白质、核酸类物质的非淀粉类α-吡喃多糖,其糖链高度在0.5 nm^1 nm。GFD-1能够显著抑制人肝癌细胞HepG2的增殖,并呈剂量依赖关系,IC_(50)为94μg/m L,但其对于正常肝脏细胞L-02无明显影响;通过AO/EB双染,经GFD-1作用的HepG2细胞呈典型凋亡形态学特征;经流式细胞仪分析,HepG2细胞被阻遏在S期。上述结果可知,初步确定分离纯化的GFD-1是一种具有抗肿瘤活性的均一多糖组分,为开发天然抗肿瘤物质和进一步分析其抗肿瘤作用机制提供了参考。展开更多
基金supported by Key Projects in National Science&Technology Pillar Program during the 12th Five-Year Plan Period(No.2012BAD33B04)Quality of Commodity Grain and Oil Resources and Processing Database Research–Grains(No.201313006)+5 种基金Key Projects of Tianjin Natural Science Foundation(No.13JCZDJC29800)863 National Plan(No.2012 AA022108)Agricultural Science and Technology Transformation Fund(No.2012GB2A100016)Plan for the Development of Changjiang Scholars and Innovative Research Team(No.IRT1166)National Nature Science Foundation of China(Nos.31000768 and 31171731)Tianjin Science and Technology Support Plan(No.10ZCZDSY 07000)
文摘The present study was designed to evaluate the immune-modulating effects of the polysaccharide from Grifola frondosa(GFP) by using mouse peritoneal macrophage and cytoxan(CTX) induced immunosuppression models. Our results from the phagocytotic and mononuclear phagocytic system function assays showed that GFP-A(one component from GFP) stimulated the phagocytosis of the phagocytes. The splenocyte proliferation assay showed that GFP-A acted the effect combing Con A or LPS in splenocyte proliferation. The results showed that GFP-A increased indices of thymus and spleen, the levels of LDH and ACP in the spleen, the m RNA levels of IL-1β, IL-2, IL-6 and IFN-γ in splenocyte. And GFP-A also significantly increased the expression of CD4^+ and CD8^+ splenic T lymphocytes, which were suppressed by the CTX in peripheral blood. In conclusion, our results indicate that the GFP-A is involved in immunomodulatory effects leading to its modulatory effects on immunosuppression.
