Three Gracilaria species, G. chouae, G. blodgettii, G. vermiculophylla and a close relative species, Gracilari-opsis lemaneiformis which is now nominated as Gracilaria lemaneiformis, are the typically indigenous spe-c...Three Gracilaria species, G. chouae, G. blodgettii, G. vermiculophylla and a close relative species, Gracilari-opsis lemaneiformis which is now nominated as Gracilaria lemaneiformis, are the typically indigenous spe-cies which are important resources for the production of special proteins, phycobilisomes, special carbo-hydrates, and agar in China. In this study, de novo transcriptome sequencing on these four species using the next generation sequencing technology was performed for the first time. Functional annotations on assembled sequencing reads showed that the transcriptomic profiles were quite different between G. lema-neiformis and other three Gracilaria species. Comparative analysis of differential gene expression related to carbohydrate and phycobiliprotein metabolisms also showed that the expression profiles of these essential genes were different in four species. The genes encoding allophycocyanin, phycocyanin and phycoerythrin were further examined in four species and their deduced amino acid sequences were used for phylogenetic analysis to confirm that G. lemaneiformis had close relationship to genus Gracilaria, as well as that within genus Gracilaria, G. chouae had closer relationship to G. vermiculophylla rather than to G. blodgettii. The de novo transcriptome study on four species provided a valuable genomic resource for further understanding and analysis on biological and evolutionary study among marine algae.展开更多
初步探讨限制性位点扩增多态性（Restriction site amplified polymorphism，RSAP）分子标记技术在龙须菜遗传多样性检测和种质鉴定上的应用。利用所优化的适合于龙须菜RSAP分析的PCR反应体系，对青岛湛山湾野生群体和栽培品系981、07-...初步探讨限制性位点扩增多态性（Restriction site amplified polymorphism，RSAP）分子标记技术在龙须菜遗传多样性检测和种质鉴定上的应用。利用所优化的适合于龙须菜RSAP分析的PCR反应体系，对青岛湛山湾野生群体和栽培品系981、07-2进行遗传多样性分析和比较。试验采用15对引物组合在3个群体14个龙须菜个体中共扩增出669个位点，其中多态位点数为146个，多态性比例22％，平均每对引物产生10条多态性条带，片段大小在100～1000bp之间。湛山湾野生群体的Na（1．0075）、Ne（1．0071）、H（O．0036）、I（0．0051）与栽培品系981的Na（1．003O）、NP（1．0021）、H（0．0012）、I（0．0018），以及栽培品系07-2的Na（1．009O）、Ne（1．0063）、H（0．0037）、I（0．0054）相比较可知3个群体的遗传多样性是有差异的。种群间的遗传多样性分析表明，在所有检测的样品中，遗传多样性多来自不同群体间的多样性。群体遗传结构分析表明，2个栽培品系981、07—2间遗传距离不大，但栽培品系与湛山湾野生群体间的遗传距离相对较大，而UPGMA聚类分析也明显的将湛山湾野生群体与栽培品系981、07—2区分开来，表明野生群体与栽培品系间已产生一定的遗传隔离。通过分析湛山湾野生群体及栽培品系981、07—2的RSAP指纹图谱，从中筛选栽培品系981的特异条带，并将其转化为稳定性好、特异性高的序列特征化扩增区（Sequence characterized amplified regions，SCAR）分子标记。展开更多
基金The National Natural Science Foundation of China under contract Nos 31140070,31271397 and 41206116the algal transcrip-tome sequencing was supported by 1KP Project(www.onekp.com)
文摘Three Gracilaria species, G. chouae, G. blodgettii, G. vermiculophylla and a close relative species, Gracilari-opsis lemaneiformis which is now nominated as Gracilaria lemaneiformis, are the typically indigenous spe-cies which are important resources for the production of special proteins, phycobilisomes, special carbo-hydrates, and agar in China. In this study, de novo transcriptome sequencing on these four species using the next generation sequencing technology was performed for the first time. Functional annotations on assembled sequencing reads showed that the transcriptomic profiles were quite different between G. lema-neiformis and other three Gracilaria species. Comparative analysis of differential gene expression related to carbohydrate and phycobiliprotein metabolisms also showed that the expression profiles of these essential genes were different in four species. The genes encoding allophycocyanin, phycocyanin and phycoerythrin were further examined in four species and their deduced amino acid sequences were used for phylogenetic analysis to confirm that G. lemaneiformis had close relationship to genus Gracilaria, as well as that within genus Gracilaria, G. chouae had closer relationship to G. vermiculophylla rather than to G. blodgettii. The de novo transcriptome study on four species provided a valuable genomic resource for further understanding and analysis on biological and evolutionary study among marine algae.
