Objective:To achieve a primary pharmacological screening contained in the aqueous extract of Berberis vulgaris(B.vulgaris) and to examine the hypoglycaemic effect and biochemical parameters of aqueous and saponins ext...Objective:To achieve a primary pharmacological screening contained in the aqueous extract of Berberis vulgaris(B.vulgaris) and to examine the hypoglycaemic effect and biochemical parameters of aqueous and saponins extract on groups of rats rendered diabetic by injection of streptozotocin.Methods:The phytochemicol tests to detect the presence of different compounds were based on the visual observation of color change or formation of precipitate after the addition of specific reagents.Diabetes was induced in rats by intraperitoneal(i.p.) injection of streptozotocin(STZ) at a dose of 65 mg/kg bw.The fasting blood glucose levels were estimated by glucose oxidase-peroxidase reactive strips(Dextrostix,Bayer Diagnostics).Blood samples were taken by cutting the tip of the tail.Serum cholesterol and serum triglycerides were estimated by enzymatic DHBS colorimetric method.Results:Administration of 62.5 and 2S.0 mg/kg of saponins and aqueous extract respectively in normal rats group shows a significant hypoglycemic activity(32.33%and 40.17%respectively) during the first week.However,diabetic group treated with saponin extract produced a maximum fall of 73.1%and 76.03%at day 1 and day 21 compared to the diabetics control.Also,blood glucose levels of the diabetic rats treated with aqueous extract showed decrease of 78.79%on the first day and the effect remains roughly constant during 3 week. Both extracts also declined significantly biochemical parameters(20.77%-49.00%).The control in the loss of body weight was observed in treated diabetic rats as compared to diabetic controls. Conclusions:These results demonstrated significant antidiabetic effects and showed that serum cholesterol and serum triglycerides levels were decreased,significantly,consequently this plant might be of value in diabetes treatment.展开更多
目的:评价根尖倒充填材料iRoot BP Plus、矿物三氧化物凝聚体、玻璃离子水门汀的封闭性能。方法:收集新鲜拔除冠根完整的32颗单根管前牙及前磨牙,随机分为三个实验组和一个阴性对照组,每组8颗离体牙。所有样本离体牙在釉牙骨质界处截冠...目的:评价根尖倒充填材料iRoot BP Plus、矿物三氧化物凝聚体、玻璃离子水门汀的封闭性能。方法:收集新鲜拔除冠根完整的32颗单根管前牙及前磨牙,随机分为三个实验组和一个阴性对照组,每组8颗离体牙。所有样本离体牙在釉牙骨质界处截冠、机械预备根管、热牙胶根管充填后在根尖3mm处截根,实验组离体牙在显微镜下根尖倒预备3mm分别以iRoot BP Plus、MTA、玻璃离子水门汀进行倒充填,待固化后所有样本离体牙去除根管内牙胶,阴性对照组保留根尖3mm牙胶,所有离体牙使用指甲油封闭。连接于改良的葡萄糖微渗漏模型,分别在1、4、7、14、20、30天检测冠方至根方渗漏的葡萄糖浓度。用SPSS18.0统计软件进行重复测量方差分析和LSD法检验,检验水准α=0.05。结果:1、4、7、14、20、30天实验组均出现不同程度葡萄糖渗漏。组内随着时间推移,三个实验组葡萄糖渗漏浓度均越来越高,组间iRoot BP Plus组与MTA组葡萄糖渗漏浓度均显著低于玻璃离子水门汀组(P<0.05),阴性对照组无葡萄糖渗漏。结论:根尖倒充填材料iRoot BP Plus、MTA封闭性均显著优于玻璃离子水门汀,而iRoot BP Plus、MTA未见明显差异。展开更多
The treatment of type 1 diabetes is mainly dependent on insulin therapy and current formulated insulin formulations are used for its control all over the world. The presented study was designed to evaluate the potency...The treatment of type 1 diabetes is mainly dependent on insulin therapy and current formulated insulin formulations are used for its control all over the world. The presented study was designed to evaluate the potency of extracted, purified and formulated insulin from the pancreatic organs of the Sudanese beef cattle. Twenty healthy rabbits were used to conduct the study following subcutaneous administration of the sample insulin, to determine the hypoglycemic effect and to analyze the potency of the testing insulin by the hypoglycemic seizure method, blood sugar method and glucose enzymatic colorimetric test (GOD-PAP) respectively. The potency of the injected insulin samples was estimated by comparing the variation in blood glucose levels produced in the treated animals with that produced by a standard insulin preparation under the suitable conditions of the blood sugar method. The results revealed that the potency of the testing beef insulin samples was slightly higher (i.e., 2.2 USP units/ml, 9%) compared to the standard and assumed potency of the prepared insulin preparations (i.e., 1-2 USP units/ml) which indicated that the solvents and diluents used to prepare the assay dilution might be of higher potency and must be diluted to such an extent that the testing insulin potency must be compatible with the standard dilutions. Furthermore, to determine the choice of an assay to analyze the potency of insulin preparations, not only the accuracy of the result but also the purpose for which the test is to be used and the time limit must be taken into consideration.展开更多
文摘Objective:To achieve a primary pharmacological screening contained in the aqueous extract of Berberis vulgaris(B.vulgaris) and to examine the hypoglycaemic effect and biochemical parameters of aqueous and saponins extract on groups of rats rendered diabetic by injection of streptozotocin.Methods:The phytochemicol tests to detect the presence of different compounds were based on the visual observation of color change or formation of precipitate after the addition of specific reagents.Diabetes was induced in rats by intraperitoneal(i.p.) injection of streptozotocin(STZ) at a dose of 65 mg/kg bw.The fasting blood glucose levels were estimated by glucose oxidase-peroxidase reactive strips(Dextrostix,Bayer Diagnostics).Blood samples were taken by cutting the tip of the tail.Serum cholesterol and serum triglycerides were estimated by enzymatic DHBS colorimetric method.Results:Administration of 62.5 and 2S.0 mg/kg of saponins and aqueous extract respectively in normal rats group shows a significant hypoglycemic activity(32.33%and 40.17%respectively) during the first week.However,diabetic group treated with saponin extract produced a maximum fall of 73.1%and 76.03%at day 1 and day 21 compared to the diabetics control.Also,blood glucose levels of the diabetic rats treated with aqueous extract showed decrease of 78.79%on the first day and the effect remains roughly constant during 3 week. Both extracts also declined significantly biochemical parameters(20.77%-49.00%).The control in the loss of body weight was observed in treated diabetic rats as compared to diabetic controls. Conclusions:These results demonstrated significant antidiabetic effects and showed that serum cholesterol and serum triglycerides levels were decreased,significantly,consequently this plant might be of value in diabetes treatment.
文摘目的:评价根尖倒充填材料iRoot BP Plus、矿物三氧化物凝聚体、玻璃离子水门汀的封闭性能。方法:收集新鲜拔除冠根完整的32颗单根管前牙及前磨牙,随机分为三个实验组和一个阴性对照组,每组8颗离体牙。所有样本离体牙在釉牙骨质界处截冠、机械预备根管、热牙胶根管充填后在根尖3mm处截根,实验组离体牙在显微镜下根尖倒预备3mm分别以iRoot BP Plus、MTA、玻璃离子水门汀进行倒充填,待固化后所有样本离体牙去除根管内牙胶,阴性对照组保留根尖3mm牙胶,所有离体牙使用指甲油封闭。连接于改良的葡萄糖微渗漏模型,分别在1、4、7、14、20、30天检测冠方至根方渗漏的葡萄糖浓度。用SPSS18.0统计软件进行重复测量方差分析和LSD法检验,检验水准α=0.05。结果:1、4、7、14、20、30天实验组均出现不同程度葡萄糖渗漏。组内随着时间推移,三个实验组葡萄糖渗漏浓度均越来越高,组间iRoot BP Plus组与MTA组葡萄糖渗漏浓度均显著低于玻璃离子水门汀组(P<0.05),阴性对照组无葡萄糖渗漏。结论:根尖倒充填材料iRoot BP Plus、MTA封闭性均显著优于玻璃离子水门汀,而iRoot BP Plus、MTA未见明显差异。
文摘The treatment of type 1 diabetes is mainly dependent on insulin therapy and current formulated insulin formulations are used for its control all over the world. The presented study was designed to evaluate the potency of extracted, purified and formulated insulin from the pancreatic organs of the Sudanese beef cattle. Twenty healthy rabbits were used to conduct the study following subcutaneous administration of the sample insulin, to determine the hypoglycemic effect and to analyze the potency of the testing insulin by the hypoglycemic seizure method, blood sugar method and glucose enzymatic colorimetric test (GOD-PAP) respectively. The potency of the injected insulin samples was estimated by comparing the variation in blood glucose levels produced in the treated animals with that produced by a standard insulin preparation under the suitable conditions of the blood sugar method. The results revealed that the potency of the testing beef insulin samples was slightly higher (i.e., 2.2 USP units/ml, 9%) compared to the standard and assumed potency of the prepared insulin preparations (i.e., 1-2 USP units/ml) which indicated that the solvents and diluents used to prepare the assay dilution might be of higher potency and must be diluted to such an extent that the testing insulin potency must be compatible with the standard dilutions. Furthermore, to determine the choice of an assay to analyze the potency of insulin preparations, not only the accuracy of the result but also the purpose for which the test is to be used and the time limit must be taken into consideration.
