Genista numidica Spach ssp numidica （Spach） Batt. is a northeast Algerian endemic plant; the aim of this work is about the phytochemical screening, the microscopic analysis and the antimicrobial activity of the aeri...Genista numidica Spach ssp numidica （Spach） Batt. is a northeast Algerian endemic plant; the aim of this work is about the phytochemical screening, the microscopic analysis and the antimicrobial activity of the aerial parts of this species （stems, flowers, seeds and pods）; there is a wealth in polyphenolic compounds： flavonoids （method of Charaux, Paris, 1964） and saponins （method of Braune et al, 1969）, tannins, terpenes, sterols and fatty acids. The microscopic analysis doesn＇t allow a differential identification of the species. The results of aromatogram by the incorporation method of Muller-Hinton on solid medium, showed a significant antimicrobial activity （method Duraffourd,1987） of the extracts, infusion, and the active substances towards bacterial strains, essentially those recognized as antibiotic resistant （Staphylococcus, Acinetobacter, Pseudomonas, Enterobacter, Citrobacter）; the results obtained indicate that the most efficient antibacterial activity was obtained with the pod infusion, the seed extract and the flavonoids. The raw extract has an important antifungal activity on Candida albicans. This could open opportunities for using this species in the treatment of urinary, respiratory, intestinals and cutaneous infections, and as a disinfecting additive on nosocomial area.展开更多
The Genisteae tribe belongs to the Fabaceae family.The wide occurrence of secondary metabolites,explicitly high-lighting the quinolizidine alkaloids(QAs),characterizes this tribe.In the present study,twenty QAs(1-20),...The Genisteae tribe belongs to the Fabaceae family.The wide occurrence of secondary metabolites,explicitly high-lighting the quinolizidine alkaloids(QAs),characterizes this tribe.In the present study,twenty QAs(1-20),including lupanine(1-7),sparteine(8-10),lupanine(11),cytisine and tetrahydrocytisine(12-17),and matrine(18-20)-type QAs were extracted and isolated from leaves of three species(i.e.,Lupinus polyphyllus(’rusell’hybrid),Lupinus muta-bilis,and Genista monspessulana)belonging to the Genisteae tribe.These plant sources were propagated under greenhouse conditions.The isolated compounds were elucidated by analyzing their spectroscopical data(MS,NMR).The antifungal effect on the mycelial growth of Fusarium oxysporum(Fox)of each isolated QA was then evaluated through the amended medium assay.The best antifungal activity was found to be for compounds 8(IC_(50)=16.5μM),9(IC_(50)=7.2μM),12(IC_(50)=11.3μM),and 18(IC_(50)=12.3μM).The inhibitory data suggest that some QAs could effi-ciently inhibit Fox mycelium growth depending on particular structural requirements deduced from structure-activity relationship scrutinies.The identified quinolizidine-related moieties can be involved in lead structures to develop further antifungal bioactives against Fox.展开更多
Genista saharae Cosson and Durieu, endemic Saharan Africa, is a spontaneous fabaceae dunes south of M＇sila （Algeria）. Perfectly adapted to arid and sandy soils, this species is a remarkable plant for dune fixation ...Genista saharae Cosson and Durieu, endemic Saharan Africa, is a spontaneous fabaceae dunes south of M＇sila （Algeria）. Perfectly adapted to arid and sandy soils, this species is a remarkable plant for dune fixation and thus fights against desertification. The objective of this study is to provide local species to various development programs of steppe ecological systems in nature and therefore ensure the rehabilitation of these species while considering the conservation of biodiversity and sustainable management of biological resources steppe. After a field survey and stratified sampling, the methodological approach has taken into account three types of analysis： a biometric analysis of a population of 100 individuals of this species, the second analysis on its biology at last an analysis of an ecological factor dealing with the accuracy governing the distribution of this species in Maiter Oued in the south of M＇sila. The results have clarified the ecology of this species to better understand its biology and to develop statistically a diagnosis on the phenology and morphology of various organs （pods, seeds, leaves） of Genista saharae Cosson and Durieu.展开更多
文摘Genista numidica Spach ssp numidica （Spach） Batt. is a northeast Algerian endemic plant; the aim of this work is about the phytochemical screening, the microscopic analysis and the antimicrobial activity of the aerial parts of this species （stems, flowers, seeds and pods）; there is a wealth in polyphenolic compounds： flavonoids （method of Charaux, Paris, 1964） and saponins （method of Braune et al, 1969）, tannins, terpenes, sterols and fatty acids. The microscopic analysis doesn＇t allow a differential identification of the species. The results of aromatogram by the incorporation method of Muller-Hinton on solid medium, showed a significant antimicrobial activity （method Duraffourd,1987） of the extracts, infusion, and the active substances towards bacterial strains, essentially those recognized as antibiotic resistant （Staphylococcus, Acinetobacter, Pseudomonas, Enterobacter, Citrobacter）; the results obtained indicate that the most efficient antibacterial activity was obtained with the pod infusion, the seed extract and the flavonoids. The raw extract has an important antifungal activity on Candida albicans. This could open opportunities for using this species in the treatment of urinary, respiratory, intestinals and cutaneous infections, and as a disinfecting additive on nosocomial area.
基金the Vicerrectoría de Investigaciones at the Universidad Militar Nueva Granada(UMNG)through the project IMP-CIAS-2924,validity 2020.
文摘The Genisteae tribe belongs to the Fabaceae family.The wide occurrence of secondary metabolites,explicitly high-lighting the quinolizidine alkaloids(QAs),characterizes this tribe.In the present study,twenty QAs(1-20),including lupanine(1-7),sparteine(8-10),lupanine(11),cytisine and tetrahydrocytisine(12-17),and matrine(18-20)-type QAs were extracted and isolated from leaves of three species(i.e.,Lupinus polyphyllus(’rusell’hybrid),Lupinus muta-bilis,and Genista monspessulana)belonging to the Genisteae tribe.These plant sources were propagated under greenhouse conditions.The isolated compounds were elucidated by analyzing their spectroscopical data(MS,NMR).The antifungal effect on the mycelial growth of Fusarium oxysporum(Fox)of each isolated QA was then evaluated through the amended medium assay.The best antifungal activity was found to be for compounds 8(IC_(50)=16.5μM),9(IC_(50)=7.2μM),12(IC_(50)=11.3μM),and 18(IC_(50)=12.3μM).The inhibitory data suggest that some QAs could effi-ciently inhibit Fox mycelium growth depending on particular structural requirements deduced from structure-activity relationship scrutinies.The identified quinolizidine-related moieties can be involved in lead structures to develop further antifungal bioactives against Fox.
文摘Genista saharae Cosson and Durieu, endemic Saharan Africa, is a spontaneous fabaceae dunes south of M＇sila （Algeria）. Perfectly adapted to arid and sandy soils, this species is a remarkable plant for dune fixation and thus fights against desertification. The objective of this study is to provide local species to various development programs of steppe ecological systems in nature and therefore ensure the rehabilitation of these species while considering the conservation of biodiversity and sustainable management of biological resources steppe. After a field survey and stratified sampling, the methodological approach has taken into account three types of analysis： a biometric analysis of a population of 100 individuals of this species, the second analysis on its biology at last an analysis of an ecological factor dealing with the accuracy governing the distribution of this species in Maiter Oued in the south of M＇sila. The results have clarified the ecology of this species to better understand its biology and to develop statistically a diagnosis on the phenology and morphology of various organs （pods, seeds, leaves） of Genista saharae Cosson and Durieu.
