The comet assay (single cell gel electrophoresis assay) was used to evaluate the radiotoxicity of Augerelectron emitters in the human osteosarcoma cell line (HOS-8603). After internal exposure to 67Ga-EDTMP, the sar-c...The comet assay (single cell gel electrophoresis assay) was used to evaluate the radiotoxicity of Augerelectron emitters in the human osteosarcoma cell line (HOS-8603). After internal exposure to 67Ga-EDTMP, the sar-coma cell has been injured severely. The comet length was longer along with the increase of dose, the appearance ofcomet tail was different from that with respect to the 60Co γ-ray irradiation. DNA damage of cell was mainly due tothe radiation effect of Auger electrons. The 67Ga may be a therapeutic radionuclide with good prospect for tumortreatment and palliation of bone pain induced by metastasis.展开更多
67Ga-EDTMP was synthesized in a single step by adding 67GaCl3 to EDTMP solution. Dependences ofthe radiolabeling yield of 67Ga-EDTMP on EDTMP concentration, pH and reaction time were examined. Under theoptimum conditi...67Ga-EDTMP was synthesized in a single step by adding 67GaCl3 to EDTMP solution. Dependences ofthe radiolabeling yield of 67Ga-EDTMP on EDTMP concentration, pH and reaction time were examined. Under theoptimum conditions, the radiolabeling yield of 67Ga-EDTMP was more than 97%. A biodistribution experiment inmice showed that 67Ga-EDTMP was mainly absorbed by skeleton and reached 13.25% at 0.5 h after injecting, thenkept a high level in 72 h with a maximum value of 16.82% at 48 h. The results suggest that 67Ga-EDTMP might be apotential bone pain palliation radiopharmaceutical due to its high skeletal uptake, rapid blood clearance and relativelylow soft-tissue absorption. But further work must be done to determine whether 67Ga-EDTMP is useful in thetreatment of painful osseous metastases.展开更多
文摘The comet assay (single cell gel electrophoresis assay) was used to evaluate the radiotoxicity of Augerelectron emitters in the human osteosarcoma cell line (HOS-8603). After internal exposure to 67Ga-EDTMP, the sar-coma cell has been injured severely. The comet length was longer along with the increase of dose, the appearance ofcomet tail was different from that with respect to the 60Co γ-ray irradiation. DNA damage of cell was mainly due tothe radiation effect of Auger electrons. The 67Ga may be a therapeutic radionuclide with good prospect for tumortreatment and palliation of bone pain induced by metastasis.
基金the National Natural Science Foundation of China(No.1 9975066)
文摘67Ga-EDTMP was synthesized in a single step by adding 67GaCl3 to EDTMP solution. Dependences ofthe radiolabeling yield of 67Ga-EDTMP on EDTMP concentration, pH and reaction time were examined. Under theoptimum conditions, the radiolabeling yield of 67Ga-EDTMP was more than 97%. A biodistribution experiment inmice showed that 67Ga-EDTMP was mainly absorbed by skeleton and reached 13.25% at 0.5 h after injecting, thenkept a high level in 72 h with a maximum value of 16.82% at 48 h. The results suggest that 67Ga-EDTMP might be apotential bone pain palliation radiopharmaceutical due to its high skeletal uptake, rapid blood clearance and relativelylow soft-tissue absorption. But further work must be done to determine whether 67Ga-EDTMP is useful in thetreatment of painful osseous metastases.
