Green-revertible albino is a novel type of chlorophyll deficiency in rice （Oryza sativa L.）, which is helpful for further research in chlorophyll synthesis and chloroplast development to illuminate their molecular m...Green-revertible albino is a novel type of chlorophyll deficiency in rice （Oryza sativa L.）, which is helpful for further research in chlorophyll synthesis and chloroplast development to illuminate their molecular mechanism. In the previous study, we had reported a single recessive gene, gra（t）, controlling this trait on the long arm of chromosome 2. In this paper, we mapped the gra（t） gene using 1,936 recessive individuals with albino phenotype in the F2 population derived from the cross between themo-photoperiod-sensitive genic male-sterile （T/PGMS） line Pei＇ai 64S and the spontaneous mutant Qiufeng M. Eventually, it was located to a confined region of 42.4 kb flanked by two microsatellite markers RM2-97 and RM13553. Based on the annotation results of RiceGAAS system, 11 open reading frames （ORFs） were predicted in this region. Among them, ORF6 was the most possible gene related to chloroplast development, which encoded the chloroplast protein synthesis elongation factor Tu in rice. Therefore, we designated it as the candidate gene of gra（t）. Sequence analysis indicated that only one base substitution C to T occurred in the coding region, which caused a missense mutation （Thr to Ile） in gra（t） mutant. These results are very valuable for further study on gra（t） gene.展开更多
Tea （Camellia sinensis） is one of the most valuable cash crops in southern China; however, the planting distribution of tea crops is not optimal and the production and cultivation regions of tea crops are restricted...Tea （Camellia sinensis） is one of the most valuable cash crops in southern China; however, the planting distribution of tea crops is not optimal and the production and cultivation regions of tea crops are restricted by law and custom. In order to evaluate the suitability of tea crops in Zhejiang Province, the annual mean temperature, the annual accumulated temperature above 10 ℃, the frequency of extremely low temperature below -13 ℃, the mean humidity from April to October, slope, aspect, altitude, soil type, and soil texture were selected from climate, topography, and soil factors as factors for land ecological evaluation by the Delphi method based on the ecological characteristics of tea crops. These nine factors were quantitatively analyzed using a geographic information system （GIS）. The grey relational analysis （GRA） was combined with the analytic hierarchy process （AHP） to address the uncertainties during the process of evaluating the traditional land ecological suitability, and a modified land ecological suitability evaluation （LESE） model was built. Based on the land-use map of Zhejiang Province, the regions that were completely unsuitable for tea cultivation in the province were eliminated and then the spatial distribution of the ecological suitability of tea crops was generated using the modified LESE model and GIS. The results demonstrated that the highly, moderately, and non-suitable regions for the cultivation of tea crops in Zhejiang Province were 27552.66, 42 724.64, and 26507.97 km2, and accounted for 28.47%, 44.14%, and 27.39% of the total evaluation area, respectively. Validation of the method showed a high degree of coincidence with the current planting distribution of tea crops in Zhejiang Province. The modified LESE model combined with GIS could be useful in quickly and accurately evaluating the land ecological suitability of tea crops, providing a scientific basis for the rational distribution of tea crops and acting as a reference to land policy makers and land use 展开更多
基金supported by the National High-tech Research and Development Program of China (863 Program) (No. 2006AA100101)the National Science and Technology Support Program (No. 2006BAD01A01-5)+1 种基金Special Program for Rice Scientific Research of Ministry of Agriculture (No. nyhyzx 07-001-006)Super Rice Breeding and Demonstration Program of Ministry of Agriculture, and Jiangsu Selfinnovation Fund for Agricultural Science and Technology (No. CX[08]603).
文摘Green-revertible albino is a novel type of chlorophyll deficiency in rice （Oryza sativa L.）, which is helpful for further research in chlorophyll synthesis and chloroplast development to illuminate their molecular mechanism. In the previous study, we had reported a single recessive gene, gra（t）, controlling this trait on the long arm of chromosome 2. In this paper, we mapped the gra（t） gene using 1,936 recessive individuals with albino phenotype in the F2 population derived from the cross between themo-photoperiod-sensitive genic male-sterile （T/PGMS） line Pei＇ai 64S and the spontaneous mutant Qiufeng M. Eventually, it was located to a confined region of 42.4 kb flanked by two microsatellite markers RM2-97 and RM13553. Based on the annotation results of RiceGAAS system, 11 open reading frames （ORFs） were predicted in this region. Among them, ORF6 was the most possible gene related to chloroplast development, which encoded the chloroplast protein synthesis elongation factor Tu in rice. Therefore, we designated it as the candidate gene of gra（t）. Sequence analysis indicated that only one base substitution C to T occurred in the coding region, which caused a missense mutation （Thr to Ile） in gra（t） mutant. These results are very valuable for further study on gra（t） gene.
基金Supported by the Agricultural Science and Technology Achievement Transformation Foundation of the Ministry of Sciences and Technology,China (No. 2008GB24160442)the National Natural Science Foundation of China (Nos. 40871158and 51108405 )
文摘Tea （Camellia sinensis） is one of the most valuable cash crops in southern China; however, the planting distribution of tea crops is not optimal and the production and cultivation regions of tea crops are restricted by law and custom. In order to evaluate the suitability of tea crops in Zhejiang Province, the annual mean temperature, the annual accumulated temperature above 10 ℃, the frequency of extremely low temperature below -13 ℃, the mean humidity from April to October, slope, aspect, altitude, soil type, and soil texture were selected from climate, topography, and soil factors as factors for land ecological evaluation by the Delphi method based on the ecological characteristics of tea crops. These nine factors were quantitatively analyzed using a geographic information system （GIS）. The grey relational analysis （GRA） was combined with the analytic hierarchy process （AHP） to address the uncertainties during the process of evaluating the traditional land ecological suitability, and a modified land ecological suitability evaluation （LESE） model was built. Based on the land-use map of Zhejiang Province, the regions that were completely unsuitable for tea cultivation in the province were eliminated and then the spatial distribution of the ecological suitability of tea crops was generated using the modified LESE model and GIS. The results demonstrated that the highly, moderately, and non-suitable regions for the cultivation of tea crops in Zhejiang Province were 27552.66, 42 724.64, and 26507.97 km2, and accounted for 28.47%, 44.14%, and 27.39% of the total evaluation area, respectively. Validation of the method showed a high degree of coincidence with the current planting distribution of tea crops in Zhejiang Province. The modified LESE model combined with GIS could be useful in quickly and accurately evaluating the land ecological suitability of tea crops, providing a scientific basis for the rational distribution of tea crops and acting as a reference to land policy makers and land use
