The occurrence and risks of Giardia in China have been unclear to date,which has made it difficult to properly manage source water as well as to create reasonable drinking water standards.The levels of Giardia in rive...The occurrence and risks of Giardia in China have been unclear to date,which has made it difficult to properly manage source water as well as to create reasonable drinking water standards.The levels of Giardia in river networks of several cities in Zhejiang Province,China were found to be in the range of 0-5 oocysts/10 L in the rainy season in 2008.The mortality due to Giardia infection for people in this region was calculated to be from 0 to 1.95 × 10？8 persons using a conditional probability equation.Based on multiple unboiled water intake routes,the disability-adjusted life years（DALYs） due to Giardia infection for people who consumed conventionally treated water was 0.625（95% CI：0.137-2.05） per 105 persons,with the symptom of hospitalization making the highest contribution to total DALYs（0.56 per 105 persons;95% CI：0.122-1.84）.The DALYs decreased to 0.425（95% CI：0.137-2.05） per 105 persons per year for those consuming water treated with advanced technology.These values were lower than the acceptable risk（1.97 × 10？5 DALYs per year）.This study revealed the risk of Giardia infection to the people in river networks of Zhejiang Province for the first time,and provides a method to evaluate the risk of Giardia infection.The results are useful for the modification of drinking water quality standards based on cost-benefit analysis.展开更多
目的初步了解临床腹泻患者贾第虫的感染情况及分子特征。方法以2014年5-7月上海市某医院临床腹泻患者为研究对象,收集粪便样本95份,进行卢戈氏碘液染色,用光学显微镜检查贾第虫包囊。采用巢式PCR法扩增贾第虫磷酸丙糖异构酶基因(tpi),...目的初步了解临床腹泻患者贾第虫的感染情况及分子特征。方法以2014年5-7月上海市某医院临床腹泻患者为研究对象,收集粪便样本95份,进行卢戈氏碘液染色,用光学显微镜检查贾第虫包囊。采用巢式PCR法扩增贾第虫磷酸丙糖异构酶基因(tpi),扩增产物测序后用BLAST、Clustal X 1.83和MEGA6.0软件进行同源性和系统发育分析。结果形态学观察发现1例腹泻患者感染贾第虫,阳性检出率为1.05%。其粪便样本经卢戈氏碘液染色后显微镜下可见清晰的包囊。对样本进行PCR检测,扩增片段大小约为530 bp,测序结果显示为贾第虫。经序列比对分析,并以tpi基因构建系统发育树,分析鉴定该分离株为集聚体B,与已报道的人源贾第虫分离株(KF271445)同源性为100%。结论临床腹泻患者中存在贾第虫感染,本研究结果可为了解贾第虫基因型特点及贾第虫病流行病学研究提供参考。展开更多
基金supported by the Foundation of Major Science and Technology Program for Water Pollution Control and Treatment (No. 2009ZX07419-002)the National Natural Science Foundation of China (No. 50778171,50809066, 20807013)
文摘The occurrence and risks of Giardia in China have been unclear to date,which has made it difficult to properly manage source water as well as to create reasonable drinking water standards.The levels of Giardia in river networks of several cities in Zhejiang Province,China were found to be in the range of 0-5 oocysts/10 L in the rainy season in 2008.The mortality due to Giardia infection for people in this region was calculated to be from 0 to 1.95 × 10？8 persons using a conditional probability equation.Based on multiple unboiled water intake routes,the disability-adjusted life years（DALYs） due to Giardia infection for people who consumed conventionally treated water was 0.625（95% CI：0.137-2.05） per 105 persons,with the symptom of hospitalization making the highest contribution to total DALYs（0.56 per 105 persons;95% CI：0.122-1.84）.The DALYs decreased to 0.425（95% CI：0.137-2.05） per 105 persons per year for those consuming water treated with advanced technology.These values were lower than the acceptable risk（1.97 × 10？5 DALYs per year）.This study revealed the risk of Giardia infection to the people in river networks of Zhejiang Province for the first time,and provides a method to evaluate the risk of Giardia infection.The results are useful for the modification of drinking water quality standards based on cost-benefit analysis.
文摘目的初步了解临床腹泻患者贾第虫的感染情况及分子特征。方法以2014年5-7月上海市某医院临床腹泻患者为研究对象,收集粪便样本95份,进行卢戈氏碘液染色,用光学显微镜检查贾第虫包囊。采用巢式PCR法扩增贾第虫磷酸丙糖异构酶基因(tpi),扩增产物测序后用BLAST、Clustal X 1.83和MEGA6.0软件进行同源性和系统发育分析。结果形态学观察发现1例腹泻患者感染贾第虫,阳性检出率为1.05%。其粪便样本经卢戈氏碘液染色后显微镜下可见清晰的包囊。对样本进行PCR检测,扩增片段大小约为530 bp,测序结果显示为贾第虫。经序列比对分析,并以tpi基因构建系统发育树,分析鉴定该分离株为集聚体B,与已报道的人源贾第虫分离株(KF271445)同源性为100%。结论临床腹泻患者中存在贾第虫感染,本研究结果可为了解贾第虫基因型特点及贾第虫病流行病学研究提供参考。
