苯扎贝特（Bezafibrate）作为新兴污染物-药品和个人护理品（Pharmaceutical and personal care products,PPCPs）的一种,被广泛关注.其在污水处理厂的污水、地表水甚至是饮用水中常被检出[1].最新研究表明它对人体有类雌激素作用[2].目...苯扎贝特（Bezafibrate）作为新兴污染物-药品和个人护理品（Pharmaceutical and personal care products,PPCPs）的一种,被广泛关注.其在污水处理厂的污水、地表水甚至是饮用水中常被检出[1].最新研究表明它对人体有类雌激素作用[2].目前,有人研究过光催化降解[1]和活性污泥转化[3]苯扎贝特的产物,但苯扎贝特在水环境中的生物降解途径及其中间产物的生态毒理效应尚不明确。展开更多
As the key point in sex hormone analysis, sample pre-treatment technology has attracted scientists' attention all over the world, and the development trend of sample preparation forwarded to faster and more effici...As the key point in sex hormone analysis, sample pre-treatment technology has attracted scientists' attention all over the world, and the development trend of sample preparation forwarded to faster and more efficient technologies. Taking economic and environmental concerns into account, subcritical fluid extraction as a faster and more efficient method has stood out as a sample pre-treatment technology. This new extraction technology can overcome the shortcomings of supercritical fluid and achieve higher extraction efficiency at relatively low pressures and temperatures. In this experiment, a simple, sensitive and efficient method has been developed for the determination of diethylstilbestrol(DES) in fish tissue using subcritical 1,1,1,2-tetrafluoroethane(R134a) extraction in combination with gas chromatography-mass spectrometry(GC-MS). After extraction, freezing-lipid filtration was utilized to remove fatty co-extract. Further purification steps were performed with C_(18) and NH_2 solid phase extraction(SPE). Finally, the analyte was derived by heptafluorobutyric anhydride(HFBA), followed by GC-MS analysis. Response surface methodology(RSM) was employed to optimizing the extraction condition, and the optimized was as follows: extraction pressure, 4.3 MPa; extraction temperature, 26℃; amount of co-solvent volume, 4.7 m L. Under this condition, at a spiked level of 1, 5, 10 μg kg^(-1), the mean recovery of DES was more than 90% with relative standard deviations(RSDs) less than 10%. Finally, the developed method has been successfully used to analyzing the real samples.展开更多
文摘苯扎贝特（Bezafibrate）作为新兴污染物-药品和个人护理品（Pharmaceutical and personal care products,PPCPs）的一种,被广泛关注.其在污水处理厂的污水、地表水甚至是饮用水中常被检出[1].最新研究表明它对人体有类雌激素作用[2].目前,有人研究过光催化降解[1]和活性污泥转化[3]苯扎贝特的产物,但苯扎贝特在水环境中的生物降解途径及其中间产物的生态毒理效应尚不明确。
基金supported by the National Natural Science Foundation of China (31071541)a program for Changjiang Scholars and Innovative Research Team in University (IRT1188)
文摘As the key point in sex hormone analysis, sample pre-treatment technology has attracted scientists' attention all over the world, and the development trend of sample preparation forwarded to faster and more efficient technologies. Taking economic and environmental concerns into account, subcritical fluid extraction as a faster and more efficient method has stood out as a sample pre-treatment technology. This new extraction technology can overcome the shortcomings of supercritical fluid and achieve higher extraction efficiency at relatively low pressures and temperatures. In this experiment, a simple, sensitive and efficient method has been developed for the determination of diethylstilbestrol(DES) in fish tissue using subcritical 1,1,1,2-tetrafluoroethane(R134a) extraction in combination with gas chromatography-mass spectrometry(GC-MS). After extraction, freezing-lipid filtration was utilized to remove fatty co-extract. Further purification steps were performed with C_(18) and NH_2 solid phase extraction(SPE). Finally, the analyte was derived by heptafluorobutyric anhydride(HFBA), followed by GC-MS analysis. Response surface methodology(RSM) was employed to optimizing the extraction condition, and the optimized was as follows: extraction pressure, 4.3 MPa; extraction temperature, 26℃; amount of co-solvent volume, 4.7 m L. Under this condition, at a spiked level of 1, 5, 10 μg kg^(-1), the mean recovery of DES was more than 90% with relative standard deviations(RSDs) less than 10%. Finally, the developed method has been successfully used to analyzing the real samples.
