Objective： To observe different effects of moxibustion at Zusanli （ST 36） and Yanglingquan （GB 34） on electrogastrogram （EGG） of patients with functional dyspepsia （FD）. Methods： A total of 65 cases were ran...Objective： To observe different effects of moxibustion at Zusanli （ST 36） and Yanglingquan （GB 34） on electrogastrogram （EGG） of patients with functional dyspepsia （FD）. Methods： A total of 65 cases were randomized into a Zusanli （ST 36） group （n=33） and a Yanglingquan （GB 34） group （n=32） by random serial number, to receive moxibustion therapy to the corresponding point respectively. The 8-lead EGG was used before and after treatment to collect EGG parameter of the gastric body, antrum of stomach, lesser curvature and greater curvature, including the dominant frequency, slow wave percentage, average frequency, response area and motility index. Results： After treatment, the dominant frequency and slow wave percentage were improved in nearly the whole stomach area in Zusanli （ST 36） group, showing statistical significances （both P〈0.05）; the slow wave percentage and average frequency were improved in stomach body and antrum of stomach in Yanglingquan （GB 34） group, showing statistical significances （both P〈0.05）; moxibustion at Zusanli （ST 36） had a significantly higher influence on EGG than Yanglingquan （GB 34）, in which the lifting of slow wave percentage was significantly higher than Yanglingquan （GB 34） group （P〈0.05）. Conclusion： Moxibustion at Zusanli （ST 36） and Yanglingquan （GB 34） has the effect of adjusting abnormal EGG pattern in FD patients. Compared with Yanglingquan （GB 34）, Zusanli （ST 36） has a superior effect in improving EGG in stomach area.展开更多
Objective: To observe the effect of electroacupuncture (EA) on Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway in knee joint synovial tissues of rats with rheumatoid arthritis (R...Objective: To observe the effect of electroacupuncture (EA) on Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway in knee joint synovial tissues of rats with rheumatoid arthritis (RA) and to explore the action mechanism of EA on RA. Methods: Twelve of the 48 SPF male Sprague-Dawley (SD) rats were assigned to a normal group by the random number table method. The remaining 36 rats were subjected to RA model preparation by intradermal injection of the Freund's complete adjuvant into the right hind foot pad of each rat under sterile conditions. After the model was successfully prepared, rats were then divided into a model group, a drug group and an EA group according to a random number table method (n=12). Rats in the drug group were treated with 2 mL aqueous solution of tripterygium glycosides [8.1 mg/(kg?bw)];rats in the EA group were treated with EA at bilateral Yanglingquan (GB 34) and Zusanli (ST 36), for 30 min each time;rats in the normal group and the model group were placed in a special rat fixation tank for 30 min each time, and received the same dose of normal saline as those in the drug group. Rats in all groups received intervention once a day for 4 weeks. Diameter of rat ankle joint and rat arthritis index were measured before and after the intervention. At the end of the experiment, the expressions of phospho-JAK2 and phospho-STAT3 were determined by immunohistochemistry. Quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect JAK2 and STAT3 mRNAs expressions. Results: After the model was produced, the arthritis index >2 was considered successful in model preparation. Compared with the model group, the ankle joint diameters and arthritis indexes of rats in the drug group and the EA group were significantly lower (all P<0.01);immunohistochemical staining cells with phospho-JAK2 and phospho-STAT3 were significantly decreased (all P<0.01);the expression levels of JAK2 and STAT3 mRNAs were decreased with statistical differences (all P<0.01). There w展开更多
AIM： To investigate the hepatoprotective effect of manual acupuncture at Yanglingquan （GB34） on CCl4-induced chronic liver damage in rats. METHODS： Rats were injected intraperitoneally with CCh （1 mL/kg） and tre...AIM： To investigate the hepatoprotective effect of manual acupuncture at Yanglingquan （GB34） on CCl4-induced chronic liver damage in rats. METHODS： Rats were injected intraperitoneally with CCh （1 mL/kg） and treated with manual acupuncture using reinforcing manipulation techniques at left GB34 （Yanglingquan） 3 times a week for 10 wk. A nonacupoint in left gluteal area was selected as a sham point. To estimate the hepatoprotective effect of manual acupuncture at GB34, measurement of liver index, biochemical assays including serum ALT, AST, ALP and total cholesterol, histological analysis and blood cell counts were conducted. RESULTS： Manual acupuncture at GB34 reduced the liver index, serum ALT, AST, ALP and total cholesterol levels as compared with the control group and the sham acupuncture group. It also increased and normalized the populations of WBC and lymphocytes. CONCLUSION： Manual acupuncture with reinforcing manipulation techniques at left GB34 reduces liver toxicity, protects liver function and liver tissue, and normalizes immune activity in CCh-intoxicated rats.展开更多
Objective： To observe the changes in numbers and degranulation status of local mast cells （MCs） of the complementary acupionts, as well as the concentrations of serum histamine and bradykinin after acupuncture at ...Objective： To observe the changes in numbers and degranulation status of local mast cells （MCs） of the complementary acupionts, as well as the concentrations of serum histamine and bradykinin after acupuncture at rat＇s complementary acupionts of Yanglingquan （GB 34） and Yinlingquan （SP 101, for exploring the mechanisms of the synergistic effect of complementary acupionts. Methods： Using random number table method, 40 Wistar rats were randomly divided into a blank control group （group K）, an acupuncture at Yanglingquan （GB 34） group （group A）, an acupuncture at Yinlingquan （SP 9） group （group B）, an acupuncture at Yanglingquan （GB 34） and Yinlingquan （SP 9） group （group AB）, and an acupuncture at points near Yanglingquan （GB 34） and Yinlingquan （SP 9） group （group CD）, 8 rats in each group. Group A received acupuncture at Yanglingquan （GB 34）; group B received acupuncture at Yinlingquan （SP 9）; group AB received acupuncture at Yanglingquan （GB 34） and Yinlingquan （SP 9）; group CD received acupuncture at the control points [points 3 mm away from Yanglingquan （GB 34） and Yinlingquan （SP 9） respectively]. Acupuncture was performed at bilateral points in each group. Rats in group K were fixed using the same method as rats in the other 4 groups without acupuncture stimulation. Needles of 0.35 mm in diameter and 40 mm in length were used for acupuncture. Needle handle was connected to the G6805-]~[ mode electroacupuncture （EA） device after needling qi was obtained, with sparse-dense wave, frequency of 2 Hz/100 Hz and current of i mA to keep the needle handles slightly tremulous while the rats kept quiet. Rats were continuously stimulated by EA for 20 rain each time. Experimental interventions were conducted on the 1st, 3rd, 5th and 7th days when the experiment started, for 4 times in total. Specimens from rats in each group were collected 2 h after the 7th day. The levels of histamine and bradykinin in serum were detected by enzyme-l展开更多
Objective To observe the clinical effect of acupuncture on the obsolete lateral malleolus joint sprain. Methods Thirty-one cases of patients with obsolete lateral malleolus joint sprain were selected. Jiexi （解溪 ST...Objective To observe the clinical effect of acupuncture on the obsolete lateral malleolus joint sprain. Methods Thirty-one cases of patients with obsolete lateral malleolus joint sprain were selected. Jiexi （解溪 ST 41） were penetrated to Shenmei （申脉 BL 61）, and Yanglingquan （阳陵泉 GB 34） was applied. The needles were retained for 30 min, and the treatment was performed only once, with follow-up visit after 1 month. Results All the patients were cured after treatment, and without relapse on the follow-up visit after 1 month. Conclusion The clinical effect of acupuncture on the obsolete lateral malleolus joint sprain is remarkable.展开更多
Objective:To observe the effect of electroacupuncture(EA)at Lower He-Sea points on the expression levels of interleukin-1β(IL-1β)in the serum and gallbladder tissues,and nuclear factor-κB(NF-κB)in gallbladder tiss...Objective:To observe the effect of electroacupuncture(EA)at Lower He-Sea points on the expression levels of interleukin-1β(IL-1β)in the serum and gallbladder tissues,and nuclear factor-κB(NF-κB)in gallbladder tissues of the guinea pigs with acute cholecystitis(AC),and to explore whether Yanglingquan(GB 34),the Lower He-Sea point pertaining to Dan Fu(gallbladder),is relatively specific for the Dan Fu(gallbladder)disorders.Methods:Eighty-two healthy guinea pigs were randomly divided into 6 groups according to the random number table method,a blank group,a model group,a Yanglingquan(GB 34)group,a Zusanli(ST 36)group,a Shangjuxu(ST 37)group,and a Xiajuxu(ST 39)group,with 12 guinea pigs in the blank group while 14 in the other groups,respectively,half males and half females in each group.Except for the blank group,guinea pigs in the other groups were injected with E.coli into the gallbladder to establish AC models.Guinea pigs in the blank group were fed routinely without special treatment;those in the model group were daily tied up for 30 min without EA treatment;those in the 4 groups receiving EA treatment were acupunctured at the corresponding Lower He-Sea points after daily binding and stimulated with the SDZ-V EA instrument.After successful modeling and treatment for 5 d,blood was collected from the abdominal aorta of the guinea pigs,and the gallbladder tissues in each group were isolated for hematoxylin-eosin(HE)staining to observe the morphological changes.Enzyme-linked immunosorbent assay(ELISA)was used to detect the serum IL-1βlevel,and immunohistochemistry(IHC)was used to detect the expression levels of NF-κB and IL-1βin gallbladder.Results:On the 3rd day after modeling,the guinea pigs in the five groups with modeling were mentally depressed with decreased appetite,significantly reduced activities,slouch,lassitude,slack and matted fur,and loose stools;two guinea pigs were selected from each group(one male and one female,not included in the final statistics)to isolate the gallbladder after sacrifice;mac展开更多
The acupoint Yanglingquan (GB 34) has the effects of normalizing the functions of the liver and gallbladder, clearing away damp-heat, and strengthening the muscles and tendons. In clinic, it is commonly used for treat...The acupoint Yanglingquan (GB 34) has the effects of normalizing the functions of the liver and gallbladder, clearing away damp-heat, and strengthening the muscles and tendons. In clinic, it is commonly used for treating hemiplegia, paralysis,pain and numbness in the lower limbs, swelling and pain in the knee, and hypochondriac pain. Through clinical practice, the author has found that this point has satisfactory therapeutic effects for treating shoulder arthritis, involuntary shaking of the head due to attack of the pathogenic wind in the craniofacial region, acute lumbar pain, and hysteric paralysis and others. These are reported in the following.展开更多
基金supported by 2013 Science and Technology Planning Project of Guangdong Provincial Hospital of Traditional Chinese Medicine,No. YK2013B1N08~~
文摘Objective： To observe different effects of moxibustion at Zusanli （ST 36） and Yanglingquan （GB 34） on electrogastrogram （EGG） of patients with functional dyspepsia （FD）. Methods： A total of 65 cases were randomized into a Zusanli （ST 36） group （n=33） and a Yanglingquan （GB 34） group （n=32） by random serial number, to receive moxibustion therapy to the corresponding point respectively. The 8-lead EGG was used before and after treatment to collect EGG parameter of the gastric body, antrum of stomach, lesser curvature and greater curvature, including the dominant frequency, slow wave percentage, average frequency, response area and motility index. Results： After treatment, the dominant frequency and slow wave percentage were improved in nearly the whole stomach area in Zusanli （ST 36） group, showing statistical significances （both P〈0.05）; the slow wave percentage and average frequency were improved in stomach body and antrum of stomach in Yanglingquan （GB 34） group, showing statistical significances （both P〈0.05）; moxibustion at Zusanli （ST 36） had a significantly higher influence on EGG than Yanglingquan （GB 34）, in which the lifting of slow wave percentage was significantly higher than Yanglingquan （GB 34） group （P〈0.05）. Conclusion： Moxibustion at Zusanli （ST 36） and Yanglingquan （GB 34） has the effect of adjusting abnormal EGG pattern in FD patients. Compared with Yanglingquan （GB 34）, Zusanli （ST 36） has a superior effect in improving EGG in stomach area.
文摘Objective: To observe the effect of electroacupuncture (EA) on Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway in knee joint synovial tissues of rats with rheumatoid arthritis (RA) and to explore the action mechanism of EA on RA. Methods: Twelve of the 48 SPF male Sprague-Dawley (SD) rats were assigned to a normal group by the random number table method. The remaining 36 rats were subjected to RA model preparation by intradermal injection of the Freund's complete adjuvant into the right hind foot pad of each rat under sterile conditions. After the model was successfully prepared, rats were then divided into a model group, a drug group and an EA group according to a random number table method (n=12). Rats in the drug group were treated with 2 mL aqueous solution of tripterygium glycosides [8.1 mg/(kg?bw)];rats in the EA group were treated with EA at bilateral Yanglingquan (GB 34) and Zusanli (ST 36), for 30 min each time;rats in the normal group and the model group were placed in a special rat fixation tank for 30 min each time, and received the same dose of normal saline as those in the drug group. Rats in all groups received intervention once a day for 4 weeks. Diameter of rat ankle joint and rat arthritis index were measured before and after the intervention. At the end of the experiment, the expressions of phospho-JAK2 and phospho-STAT3 were determined by immunohistochemistry. Quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect JAK2 and STAT3 mRNAs expressions. Results: After the model was produced, the arthritis index >2 was considered successful in model preparation. Compared with the model group, the ankle joint diameters and arthritis indexes of rats in the drug group and the EA group were significantly lower (all P<0.01);immunohistochemical staining cells with phospho-JAK2 and phospho-STAT3 were significantly decreased (all P<0.01);the expression levels of JAK2 and STAT3 mRNAs were decreased with statistical differences (all P<0.01). There w
文摘AIM： To investigate the hepatoprotective effect of manual acupuncture at Yanglingquan （GB34） on CCl4-induced chronic liver damage in rats. METHODS： Rats were injected intraperitoneally with CCh （1 mL/kg） and treated with manual acupuncture using reinforcing manipulation techniques at left GB34 （Yanglingquan） 3 times a week for 10 wk. A nonacupoint in left gluteal area was selected as a sham point. To estimate the hepatoprotective effect of manual acupuncture at GB34, measurement of liver index, biochemical assays including serum ALT, AST, ALP and total cholesterol, histological analysis and blood cell counts were conducted. RESULTS： Manual acupuncture at GB34 reduced the liver index, serum ALT, AST, ALP and total cholesterol levels as compared with the control group and the sham acupuncture group. It also increased and normalized the populations of WBC and lymphocytes. CONCLUSION： Manual acupuncture with reinforcing manipulation techniques at left GB34 reduces liver toxicity, protects liver function and liver tissue, and normalizes immune activity in CCh-intoxicated rats.
基金supported by Project of Natural Science Foundation of Hebei Province,No.H2014406047Key Program of Science and Technology of Hebei Higher Education,No.ZD20131001~~
文摘Objective： To observe the changes in numbers and degranulation status of local mast cells （MCs） of the complementary acupionts, as well as the concentrations of serum histamine and bradykinin after acupuncture at rat＇s complementary acupionts of Yanglingquan （GB 34） and Yinlingquan （SP 101, for exploring the mechanisms of the synergistic effect of complementary acupionts. Methods： Using random number table method, 40 Wistar rats were randomly divided into a blank control group （group K）, an acupuncture at Yanglingquan （GB 34） group （group A）, an acupuncture at Yinlingquan （SP 9） group （group B）, an acupuncture at Yanglingquan （GB 34） and Yinlingquan （SP 9） group （group AB）, and an acupuncture at points near Yanglingquan （GB 34） and Yinlingquan （SP 9） group （group CD）, 8 rats in each group. Group A received acupuncture at Yanglingquan （GB 34）; group B received acupuncture at Yinlingquan （SP 9）; group AB received acupuncture at Yanglingquan （GB 34） and Yinlingquan （SP 9）; group CD received acupuncture at the control points [points 3 mm away from Yanglingquan （GB 34） and Yinlingquan （SP 9） respectively]. Acupuncture was performed at bilateral points in each group. Rats in group K were fixed using the same method as rats in the other 4 groups without acupuncture stimulation. Needles of 0.35 mm in diameter and 40 mm in length were used for acupuncture. Needle handle was connected to the G6805-]~[ mode electroacupuncture （EA） device after needling qi was obtained, with sparse-dense wave, frequency of 2 Hz/100 Hz and current of i mA to keep the needle handles slightly tremulous while the rats kept quiet. Rats were continuously stimulated by EA for 20 rain each time. Experimental interventions were conducted on the 1st, 3rd, 5th and 7th days when the experiment started, for 4 times in total. Specimens from rats in each group were collected 2 h after the 7th day. The levels of histamine and bradykinin in serum were detected by enzyme-l
文摘Objective To observe the clinical effect of acupuncture on the obsolete lateral malleolus joint sprain. Methods Thirty-one cases of patients with obsolete lateral malleolus joint sprain were selected. Jiexi （解溪 ST 41） were penetrated to Shenmei （申脉 BL 61）, and Yanglingquan （阳陵泉 GB 34） was applied. The needles were retained for 30 min, and the treatment was performed only once, with follow-up visit after 1 month. Results All the patients were cured after treatment, and without relapse on the follow-up visit after 1 month. Conclusion The clinical effect of acupuncture on the obsolete lateral malleolus joint sprain is remarkable.
文摘Objective:To observe the effect of electroacupuncture(EA)at Lower He-Sea points on the expression levels of interleukin-1β(IL-1β)in the serum and gallbladder tissues,and nuclear factor-κB(NF-κB)in gallbladder tissues of the guinea pigs with acute cholecystitis(AC),and to explore whether Yanglingquan(GB 34),the Lower He-Sea point pertaining to Dan Fu(gallbladder),is relatively specific for the Dan Fu(gallbladder)disorders.Methods:Eighty-two healthy guinea pigs were randomly divided into 6 groups according to the random number table method,a blank group,a model group,a Yanglingquan(GB 34)group,a Zusanli(ST 36)group,a Shangjuxu(ST 37)group,and a Xiajuxu(ST 39)group,with 12 guinea pigs in the blank group while 14 in the other groups,respectively,half males and half females in each group.Except for the blank group,guinea pigs in the other groups were injected with E.coli into the gallbladder to establish AC models.Guinea pigs in the blank group were fed routinely without special treatment;those in the model group were daily tied up for 30 min without EA treatment;those in the 4 groups receiving EA treatment were acupunctured at the corresponding Lower He-Sea points after daily binding and stimulated with the SDZ-V EA instrument.After successful modeling and treatment for 5 d,blood was collected from the abdominal aorta of the guinea pigs,and the gallbladder tissues in each group were isolated for hematoxylin-eosin(HE)staining to observe the morphological changes.Enzyme-linked immunosorbent assay(ELISA)was used to detect the serum IL-1βlevel,and immunohistochemistry(IHC)was used to detect the expression levels of NF-κB and IL-1βin gallbladder.Results:On the 3rd day after modeling,the guinea pigs in the five groups with modeling were mentally depressed with decreased appetite,significantly reduced activities,slouch,lassitude,slack and matted fur,and loose stools;two guinea pigs were selected from each group(one male and one female,not included in the final statistics)to isolate the gallbladder after sacrifice;mac
文摘The acupoint Yanglingquan (GB 34) has the effects of normalizing the functions of the liver and gallbladder, clearing away damp-heat, and strengthening the muscles and tendons. In clinic, it is commonly used for treating hemiplegia, paralysis,pain and numbness in the lower limbs, swelling and pain in the knee, and hypochondriac pain. Through clinical practice, the author has found that this point has satisfactory therapeutic effects for treating shoulder arthritis, involuntary shaking of the head due to attack of the pathogenic wind in the craniofacial region, acute lumbar pain, and hysteric paralysis and others. These are reported in the following.
