本文对大豆种间杂交(Glycine max (L) Merril×G. soja Sieb. and Zucc., G. max×G.gracilis Skvok Tzow)后代的性状表现及基因效应进行了研究,并初步分析了以栽培大豆为轮回亲本的回交效应。结果表明:F_1代育性不完全正常。...本文对大豆种间杂交(Glycine max (L) Merril×G. soja Sieb. and Zucc., G. max×G.gracilis Skvok Tzow)后代的性状表现及基因效应进行了研究,并初步分析了以栽培大豆为轮回亲本的回交效应。结果表明:F_1代育性不完全正常。上位性基因效应是普遍存在的、不可忽视的重要遗传组成成分。种间杂交后代分离广泛,类型丰富。利用野生资源成败的关键在于亲本选配。回交是利用野生资源的一条有效途径。半野生大豆的价值不容忽视。展开更多
To explore genetic resource of wild soybean(Glycine soia. L), RNA-seq was used to investigate cyst nematode resistance of G. soja. Root transcriptome expressions were profiled at 9, 15 and 20 d post inoculation(DPI) i...To explore genetic resource of wild soybean(Glycine soia. L), RNA-seq was used to investigate cyst nematode resistance of G. soja. Root transcriptome expressions were profiled at 9, 15 and 20 d post inoculation(DPI) in resistant and susceptible G. soja to SCN(soybean cyst nematode). A total of 1,594 differentially expressed genes(DEGs) were identified in roots infected by SCN compared with non-infected roots. In the resistant accession, 619, 65, and 8 DEGs were detected at 9, 15, and 20 DPI, respectively, while 327, 460 and 115 DEGs were detected at the same sampling point of susceptible accessions. DEGs were enriched in peroxidase gene sets which were involved in response to oxidative stress and oxidation reduction. Two gene families, ZIM transcription factor and WRKY transcription factor were enriched. WRKY transcription factor was only enriched in resistant accession. Moreover, gene expressions of 9 DEGs were validated by qRT-PCR. XLOC_023202, an unknown protein was up regulated more than 5 fold at 9 and 15 DPI in the resistant accession. These results provided an atlas of gene expressions of G. soja in response to SCN infection, and identified candidate DEGs for future research.展开更多
文摘本文对大豆种间杂交(Glycine max (L) Merril×G. soja Sieb. and Zucc., G. max×G.gracilis Skvok Tzow)后代的性状表现及基因效应进行了研究,并初步分析了以栽培大豆为轮回亲本的回交效应。结果表明:F_1代育性不完全正常。上位性基因效应是普遍存在的、不可忽视的重要遗传组成成分。种间杂交后代分离广泛,类型丰富。利用野生资源成败的关键在于亲本选配。回交是利用野生资源的一条有效途径。半野生大豆的价值不容忽视。
基金supported by Science and Technology Development Program of Jilin Province (20170414009GH)Agricultural Science and Technology Innovation Project of Jilin Province (CXGC2017JQ018, CXGC2017ZY024)the United States Department of Agriculture-Agricultural Research Service
文摘To explore genetic resource of wild soybean(Glycine soia. L), RNA-seq was used to investigate cyst nematode resistance of G. soja. Root transcriptome expressions were profiled at 9, 15 and 20 d post inoculation(DPI) in resistant and susceptible G. soja to SCN(soybean cyst nematode). A total of 1,594 differentially expressed genes(DEGs) were identified in roots infected by SCN compared with non-infected roots. In the resistant accession, 619, 65, and 8 DEGs were detected at 9, 15, and 20 DPI, respectively, while 327, 460 and 115 DEGs were detected at the same sampling point of susceptible accessions. DEGs were enriched in peroxidase gene sets which were involved in response to oxidative stress and oxidation reduction. Two gene families, ZIM transcription factor and WRKY transcription factor were enriched. WRKY transcription factor was only enriched in resistant accession. Moreover, gene expressions of 9 DEGs were validated by qRT-PCR. XLOC_023202, an unknown protein was up regulated more than 5 fold at 9 and 15 DPI in the resistant accession. These results provided an atlas of gene expressions of G. soja in response to SCN infection, and identified candidate DEGs for future research.
