Atherosclerosis(AS)is a chronic inflammatory disease,the main causes of which include abnormal lipid metabolism,endothelial injury,physical and chemical injury,hemodynamic injury,genetic factors and so on.These causes...Atherosclerosis(AS)is a chronic inflammatory disease,the main causes of which include abnormal lipid metabolism,endothelial injury,physical and chemical injury,hemodynamic injury,genetic factors and so on.These causes can lead to inflammatory injury of blood vessels and local dysfunction.Bunao-Fuyuan decoction(BNFY)is a traditional Chinese medicine compound that can treat cardiovascular and cerebrovascular diseases,but its effect on AS is still unknown.The aim of this study was to investigate the effect and mechanism of BNFY in proliferation and migration of vascular smooth muscle cells(VSMCs)on AS.At first,the expression ofα-SMA protein in ox-LDL-induced VSMCs,which was detected by immunofluorescence staining and western blot.CCK-8 technique and cloning technique were used to detect the cell proliferation of ox-LDL-induced VSMCs after adding BNFY.Meanwhile,the expression of proliferating protein Ki67 was detected by immunofluorescence staining.Western blot was also used to detect the expression of proliferation-related proteins CDK2,CyclinE1 and P27.Flow cytometry was used to detect the effect of BNFY on cell cycle.The effects of BNFY on proliferation and migration of cells were detected by cell scratch test and Transwell.Western blot was used to detect the expression of adhesion factors ICAM1,VCAM1,muc1,VE-cadherin and RHOA/ROCK-related proteins in cells.We found that the expression of AS markerα-SMA protein increased significantly and cells shriveled and a few floated on the medium after induction of ox-LDL on VSCMs.The proliferation rate of ox-LDL VSMCs decreased significantly after adding different doses of BNFY,and BNFY can inhibit cell cycle.Meanwhile,we also found that cell invasion and migration rate were significantly inhibited and related cell adhesion factors ICAM1,VCAM1,muc1 and VE-cadherin were inhibited too by BNFY.Finally,we found that BNFY inhibited the expression of RHOA,ROCK1,ROCK2,p-MLC proteins in the RHOA/ROCK signaling pathway.Therefore,we can summarize that BNFY may inhibit the prolifera展开更多
目的:观察复元汤联合西药治疗脑梗死的临床疗效。方法:将97例患者按照数字表法随机分为对照组42例和观察组55例,对照组给予西药疏血通、阿司匹林、甘露醇治疗,观察组在对照组基础上加用复元汤治疗,两组患者均以30 d为1个疗程,1个疗程后...目的:观察复元汤联合西药治疗脑梗死的临床疗效。方法:将97例患者按照数字表法随机分为对照组42例和观察组55例,对照组给予西药疏血通、阿司匹林、甘露醇治疗,观察组在对照组基础上加用复元汤治疗,两组患者均以30 d为1个疗程,1个疗程后比较两组患者的临床疗效、神经功能缺损评分、中风证候积分、美国国立卫生研究院卒中量表(national institute of health stroke scale,NIHSS)评分和日常生活活动能力量表(activity of daily living scale,ADL)评分。结果:对照组痊愈6例,显效12例,有效14例,无效10例,有效率为76.19%;观察组痊愈15例,显效16例,有效20例,无效4例,有效率为92.73%,两组有效率比较,差异均有统计学意义(P<0.05)。两组治疗后神经功能缺损评分和中风证候积分与治疗前比较,差异均有统计学意义(P<0.05);观察组神经功能缺损评分和中风证候积分与对照组比较,差异均有统计学意义(P<0.05)。两组治疗后NIHSS评分和ADL评分与治疗前比较,差异均有统计学意义(P<0.05),观察组NIHSS评分和ADL评分与对照组比较,差异均有统计学意义(P<0.05)。结论:复元汤结合西药治疗脑梗死疗效显著,且无不良反应。展开更多
OBJECTIVE:To investigate the protective efficacy of Bunao Fuyuan decoction(BNFY)on cerebral Ischemia/reperfusion(I/R)injury.METHODS:The mouse PC12 cells were chosen,and the oxidative-glucose deprivation/re-oxygenation...OBJECTIVE:To investigate the protective efficacy of Bunao Fuyuan decoction(BNFY)on cerebral Ischemia/reperfusion(I/R)injury.METHODS:The mouse PC12 cells were chosen,and the oxidative-glucose deprivation/re-oxygenation(OGD/R)injury model were established to simulate cerebral I/R injury.Atorvastatin was selected as a positive drug,and a gradient dose of BNFY was given for 6,12 and 24 h.3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT)assay were used to detect cell viability at each time point.Cell apoptosis was measured by terminal deoxynucleotidyl transferase-mediated d UTP-botin nick end labeling(TUNEL)staining.enzyme linked immunosorbent assay was used to detect the expression of tumor necrosis factor(TNF)-α,interleukin(IL)-6,IL-1βand platelet activating factor(PAF).Western blot assay were performed to detect the expression of key regulators[toll-like receptor 4(TLR4),nuclear factor kappa-B(NF-κB),p-p38 mito-gen-activated protein kinase(MAPK)and p-Akt(also known as protein kinase B,PKB)]of cell survival and inflammatory response.RESULTS:The results of MTT assay and TUNEL staining assay revealed that BNFY significantly increased cell viability and inhibited cell apoptosis of PC12 cells following OGD/R,respectively.Furthermore,the expression of TNF-α,1 L-6,1 L-1βand PAF were decreased after BNFY treatment.And the results of Western blot assay showed that BNFY downregulated TLR4,NF-κB,p-p38 MAPK expression and upregulated p-Akt expression.CONCLUSION:Our findings suggest that BNFY may play a role in protecting OGD/R injured PC12 cells through inhibiting the inflammatory response and cell apoptosis.展开更多
According to the report of the National Cancer Center,there are about 4.5 million new cancer patients and 3 million deaths in China every year.How to effectively implement a comprehensive system of prevention and cont...According to the report of the National Cancer Center,there are about 4.5 million new cancer patients and 3 million deaths in China every year.How to effectively implement a comprehensive system of prevention and control measures and reduce the incidence rate of malignant tumors in the country,so that the mortality rate of cancer is effectively controlled is a problem that needs to be solved urgently.In particular,it is worth mentioning that in China,the addition of traditional Chinese medicine in the treatment of malignant tumor plays an important role in improving the therapeutic effect,reducing the toxic and side effects of comprehensive treatment,improving the quality of life,and"survival with tumor"of end-stage cancer patients.Professor Wang Xisheng has been engaged in the field of traditional Chinese medicine in the treatment of cancer for more than 40 years.In his long-term clinical practice,he has accumulated rich experience in the prevention and treatment of malignant tumors.This article introduces Professor Wang's thinking on cancer in clinical practice and the"Fu Yuan Fu Heng method"in the treatment of malignant tumors for reference.展开更多
基金supported by the Natural Science Foundation of Zhejiang Province,China(No.LY16H020010)Medicine Health Science and Technology Plan of Zhejiang Province(No.2017194804)Science and Technology Bureau of Wenzhou(No.Y20160021)。
文摘Atherosclerosis(AS)is a chronic inflammatory disease,the main causes of which include abnormal lipid metabolism,endothelial injury,physical and chemical injury,hemodynamic injury,genetic factors and so on.These causes can lead to inflammatory injury of blood vessels and local dysfunction.Bunao-Fuyuan decoction(BNFY)is a traditional Chinese medicine compound that can treat cardiovascular and cerebrovascular diseases,but its effect on AS is still unknown.The aim of this study was to investigate the effect and mechanism of BNFY in proliferation and migration of vascular smooth muscle cells(VSMCs)on AS.At first,the expression ofα-SMA protein in ox-LDL-induced VSMCs,which was detected by immunofluorescence staining and western blot.CCK-8 technique and cloning technique were used to detect the cell proliferation of ox-LDL-induced VSMCs after adding BNFY.Meanwhile,the expression of proliferating protein Ki67 was detected by immunofluorescence staining.Western blot was also used to detect the expression of proliferation-related proteins CDK2,CyclinE1 and P27.Flow cytometry was used to detect the effect of BNFY on cell cycle.The effects of BNFY on proliferation and migration of cells were detected by cell scratch test and Transwell.Western blot was used to detect the expression of adhesion factors ICAM1,VCAM1,muc1,VE-cadherin and RHOA/ROCK-related proteins in cells.We found that the expression of AS markerα-SMA protein increased significantly and cells shriveled and a few floated on the medium after induction of ox-LDL on VSCMs.The proliferation rate of ox-LDL VSMCs decreased significantly after adding different doses of BNFY,and BNFY can inhibit cell cycle.Meanwhile,we also found that cell invasion and migration rate were significantly inhibited and related cell adhesion factors ICAM1,VCAM1,muc1 and VE-cadherin were inhibited too by BNFY.Finally,we found that BNFY inhibited the expression of RHOA,ROCK1,ROCK2,p-MLC proteins in the RHOA/ROCK signaling pathway.Therefore,we can summarize that BNFY may inhibit the prolifera
文摘目的:观察复元汤联合西药治疗脑梗死的临床疗效。方法:将97例患者按照数字表法随机分为对照组42例和观察组55例,对照组给予西药疏血通、阿司匹林、甘露醇治疗,观察组在对照组基础上加用复元汤治疗,两组患者均以30 d为1个疗程,1个疗程后比较两组患者的临床疗效、神经功能缺损评分、中风证候积分、美国国立卫生研究院卒中量表(national institute of health stroke scale,NIHSS)评分和日常生活活动能力量表(activity of daily living scale,ADL)评分。结果:对照组痊愈6例,显效12例,有效14例,无效10例,有效率为76.19%;观察组痊愈15例,显效16例,有效20例,无效4例,有效率为92.73%,两组有效率比较,差异均有统计学意义(P<0.05)。两组治疗后神经功能缺损评分和中风证候积分与治疗前比较,差异均有统计学意义(P<0.05);观察组神经功能缺损评分和中风证候积分与对照组比较,差异均有统计学意义(P<0.05)。两组治疗后NIHSS评分和ADL评分与治疗前比较,差异均有统计学意义(P<0.05),观察组NIHSS评分和ADL评分与对照组比较,差异均有统计学意义(P<0.05)。结论:复元汤结合西药治疗脑梗死疗效显著,且无不良反应。
文摘OBJECTIVE:To investigate the protective efficacy of Bunao Fuyuan decoction(BNFY)on cerebral Ischemia/reperfusion(I/R)injury.METHODS:The mouse PC12 cells were chosen,and the oxidative-glucose deprivation/re-oxygenation(OGD/R)injury model were established to simulate cerebral I/R injury.Atorvastatin was selected as a positive drug,and a gradient dose of BNFY was given for 6,12 and 24 h.3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT)assay were used to detect cell viability at each time point.Cell apoptosis was measured by terminal deoxynucleotidyl transferase-mediated d UTP-botin nick end labeling(TUNEL)staining.enzyme linked immunosorbent assay was used to detect the expression of tumor necrosis factor(TNF)-α,interleukin(IL)-6,IL-1βand platelet activating factor(PAF).Western blot assay were performed to detect the expression of key regulators[toll-like receptor 4(TLR4),nuclear factor kappa-B(NF-κB),p-p38 mito-gen-activated protein kinase(MAPK)and p-Akt(also known as protein kinase B,PKB)]of cell survival and inflammatory response.RESULTS:The results of MTT assay and TUNEL staining assay revealed that BNFY significantly increased cell viability and inhibited cell apoptosis of PC12 cells following OGD/R,respectively.Furthermore,the expression of TNF-α,1 L-6,1 L-1βand PAF were decreased after BNFY treatment.And the results of Western blot assay showed that BNFY downregulated TLR4,NF-κB,p-p38 MAPK expression and upregulated p-Akt expression.CONCLUSION:Our findings suggest that BNFY may play a role in protecting OGD/R injured PC12 cells through inhibiting the inflammatory response and cell apoptosis.
文摘According to the report of the National Cancer Center,there are about 4.5 million new cancer patients and 3 million deaths in China every year.How to effectively implement a comprehensive system of prevention and control measures and reduce the incidence rate of malignant tumors in the country,so that the mortality rate of cancer is effectively controlled is a problem that needs to be solved urgently.In particular,it is worth mentioning that in China,the addition of traditional Chinese medicine in the treatment of malignant tumor plays an important role in improving the therapeutic effect,reducing the toxic and side effects of comprehensive treatment,improving the quality of life,and"survival with tumor"of end-stage cancer patients.Professor Wang Xisheng has been engaged in the field of traditional Chinese medicine in the treatment of cancer for more than 40 years.In his long-term clinical practice,he has accumulated rich experience in the prevention and treatment of malignant tumors.This article introduces Professor Wang's thinking on cancer in clinical practice and the"Fu Yuan Fu Heng method"in the treatment of malignant tumors for reference.
