蛋白质功能的准确预测有利于推进生物医学发展,高通量测序技术的快速发展加快了蛋白质序列的提取速度,从而产生了大量未注释的蛋白质,并且新测序序列缺乏结构等生物信息,针对该问题提出了基于序列和组合图卷积网络的蛋白质功能预测模型(...蛋白质功能的准确预测有利于推进生物医学发展,高通量测序技术的快速发展加快了蛋白质序列的提取速度,从而产生了大量未注释的蛋白质,并且新测序序列缺乏结构等生物信息,针对该问题提出了基于序列和组合图卷积网络的蛋白质功能预测模型(Protein Function Prediction using Sequences and Combined Graph Convolutional Networks, PFP-SCGCN).首先通过深度学习方法捕获蛋白质序列的多维特征信息,再通过多序列比对从蛋白质序列中提取进化耦合信息和氨基酸残基群落,然后利用进化耦合信息和氨基酸残基群落生成序列氨基酸之间两种不同连接程度的邻接矩阵,将这两种邻接矩阵与序列特征信息一起输入给组合图卷积网络进行信息融合,最后通过多个全连接层获得蛋白质功能类别信息.本文还通过分析PFP-SCGCN的特定网络层识别蛋白质功能位点,可帮助人们推测出新序列中的重要氨基酸.模型结果表明,PFP-SCGCN模型的功能预测准确率远高于对比方法,具有较好的鲁棒性,并且可以较准确的识别功能位点.展开更多
Metal-organic frameworks（MOFs） are a unique class of porous crystalline materials that have shown promise for a wide range of applications. MOFs have been explored as a new type of heterogeneous catalytic materials,...Metal-organic frameworks（MOFs） are a unique class of porous crystalline materials that have shown promise for a wide range of applications. MOFs have been explored as a new type of heterogeneous catalytic materials, because of their high surface area, uniform and tunable pores, facile functionalization and incorporation of catalytic active sites. The use of multi-functional sites MOF materials as catalysts for synergistic catalysis and tandem reactions has attracted increasing attention. In this review, we aim to introduce the construction of bi-or multi-functional MOF catalysts with cooperative or cascade functions via post-synthetic modification（PSM）.展开更多
Rebuilding the damaged motor function caused by spinal cord injury is one of the most serious challenges in clinical neuroscience.The function of the neural pathway under the damaged sites can be rebuilt using functio...Rebuilding the damaged motor function caused by spinal cord injury is one of the most serious challenges in clinical neuroscience.The function of the neural pathway under the damaged sites can be rebuilt using functional electrical stimulation technology.In this study,the locations of motor function sites in the lumbosacral spinal cord were determined with functional electrical stimulation technology.A three-dimensional map of the lumbosacral spinal cord comprising the relationship between the motor function sites and the corresponding muscle was drawn.Based on the individual experimental parameters and normalized coordinates of the motor function sites,the motor function sites that control a certain muscle were calculated.Phasing pulse sequences were delivered to the determined motor function sites in the spinal cord and hip extension,hip flexion,ankle plantarflexion,and ankle dorsiflexion movements were successfully achieved.The results show that the map of the spinal cord motor function sites was valid.This map can provide guidance for the selection of electrical stimulation sites during the rebuilding of motor function after spinal cord injury.展开更多
文摘蛋白质功能的准确预测有利于推进生物医学发展,高通量测序技术的快速发展加快了蛋白质序列的提取速度,从而产生了大量未注释的蛋白质,并且新测序序列缺乏结构等生物信息,针对该问题提出了基于序列和组合图卷积网络的蛋白质功能预测模型(Protein Function Prediction using Sequences and Combined Graph Convolutional Networks, PFP-SCGCN).首先通过深度学习方法捕获蛋白质序列的多维特征信息,再通过多序列比对从蛋白质序列中提取进化耦合信息和氨基酸残基群落,然后利用进化耦合信息和氨基酸残基群落生成序列氨基酸之间两种不同连接程度的邻接矩阵,将这两种邻接矩阵与序列特征信息一起输入给组合图卷积网络进行信息融合,最后通过多个全连接层获得蛋白质功能类别信息.本文还通过分析PFP-SCGCN的特定网络层识别蛋白质功能位点,可帮助人们推测出新序列中的重要氨基酸.模型结果表明,PFP-SCGCN模型的功能预测准确率远高于对比方法,具有较好的鲁棒性,并且可以较准确的识别功能位点.
基金supported by the National Natural Science Foundation of China (Nos. 21371069 and 21621001)
文摘Metal-organic frameworks（MOFs） are a unique class of porous crystalline materials that have shown promise for a wide range of applications. MOFs have been explored as a new type of heterogeneous catalytic materials, because of their high surface area, uniform and tunable pores, facile functionalization and incorporation of catalytic active sites. The use of multi-functional sites MOF materials as catalysts for synergistic catalysis and tandem reactions has attracted increasing attention. In this review, we aim to introduce the construction of bi-or multi-functional MOF catalysts with cooperative or cascade functions via post-synthetic modification（PSM）.
基金supported by the National Natural Science Foundation of China,No.81371663,61534003the Top-notch Academic Programs Project of Jiangsu Higher Education Institutions of China,No.PPZY2015B135
文摘Rebuilding the damaged motor function caused by spinal cord injury is one of the most serious challenges in clinical neuroscience.The function of the neural pathway under the damaged sites can be rebuilt using functional electrical stimulation technology.In this study,the locations of motor function sites in the lumbosacral spinal cord were determined with functional electrical stimulation technology.A three-dimensional map of the lumbosacral spinal cord comprising the relationship between the motor function sites and the corresponding muscle was drawn.Based on the individual experimental parameters and normalized coordinates of the motor function sites,the motor function sites that control a certain muscle were calculated.Phasing pulse sequences were delivered to the determined motor function sites in the spinal cord and hip extension,hip flexion,ankle plantarflexion,and ankle dorsiflexion movements were successfully achieved.The results show that the map of the spinal cord motor function sites was valid.This map can provide guidance for the selection of electrical stimulation sites during the rebuilding of motor function after spinal cord injury.
