In the present study, we aimed to explore the mechanism of Fu-Zi Decoction in the treatment of chronic heart failure(CHF) using network pharmacology. Sym Map database was used to analyze the modern medical(MM) symptom...In the present study, we aimed to explore the mechanism of Fu-Zi Decoction in the treatment of chronic heart failure(CHF) using network pharmacology. Sym Map database was used to analyze the modern medical(MM) symptoms of various medicines. The chemical components of Fu-Zi Decoction were obtained through TCMSP, ETCM database, and previous results. The targets of Fu-Zi Decoction were obtained through STITCH, Swiss Target Prediction, Target NET database, and literature. The targets for the treatment of CHF were obtained from the Dis Ge NET, GEO, and Drug Bank databases, and the common parts of the Fu-Zi Decoction targets were screened out to construct the PPI network. The PPI network was decomposed modularly, its functions were analyzed, and the KEGG pathway enrichment analysis was performed. The key target was verified by Swiss Dock for molecular docking. A total of 205 chemical components of Fu-Zi Decoction, 551 drug targets, and 521 disease targets were collected. Functional enrichment analysis revealed that it was mainly involved in biological processes, such as negative regulation of cell death, oxidative stress, and G protein-coupled receptor regulation. KEGG enrichment findings mainly involved fluid shear stress and atherosclerosis, IL-17 signaling pathway, and so on. The results of molecular docking showed that benzoylaconitine, aconitine, mesaconitine, paeoniflorin, and atractylodes Ⅲ all had a strong affinity with the core target CXCL8, suggesting that Fu-Zi Decoction could negatively regulate cell apoptosis and oxidative stress through fluid shear stress and atherosclerosis, IL-17 signaling pathway, and so on. Collectively, our data showed that Fu-Zi Decoction had a good effect on the treatment of CHF.展开更多
Four structurally unprecedented aconitane-type C_（19）-diterpenoid alkaloid glycosides with isomeric arabinosyls, named aconicarmichosides A–D（1–4）, were isolated from an aqueous extract of ＂fu zi＂, the lateral...Four structurally unprecedented aconitane-type C_（19）-diterpenoid alkaloid glycosides with isomeric arabinosyls, named aconicarmichosides A–D（1–4）, were isolated from an aqueous extract of ＂fu zi＂, the lateral roots of Aconitum carmichaelii. Their structures were determined as neoline 14-O-a-and 14-O-b-L-arabinopyranosides（1 and 2） and 14-O-a-and 14-O-b-L-arabinofuranosides（3 and 4）, by spectroscopic and chemical methods including 2D NMR experiments and acid hydrolysis. Compounds 1–4 represent the first examples of glycosidic diterpenoid alkaloids.展开更多
基金Shanxi Science and Technology Department(Grant No.2015011103)Shanxi Science and Technology Department(Grant No.201901D111343)“Research and development of Shanxi medicinal tea”of Shanxi University of traditional Chinese Medicine(Grant No.2020PY-YC-10)。
文摘In the present study, we aimed to explore the mechanism of Fu-Zi Decoction in the treatment of chronic heart failure(CHF) using network pharmacology. Sym Map database was used to analyze the modern medical(MM) symptoms of various medicines. The chemical components of Fu-Zi Decoction were obtained through TCMSP, ETCM database, and previous results. The targets of Fu-Zi Decoction were obtained through STITCH, Swiss Target Prediction, Target NET database, and literature. The targets for the treatment of CHF were obtained from the Dis Ge NET, GEO, and Drug Bank databases, and the common parts of the Fu-Zi Decoction targets were screened out to construct the PPI network. The PPI network was decomposed modularly, its functions were analyzed, and the KEGG pathway enrichment analysis was performed. The key target was verified by Swiss Dock for molecular docking. A total of 205 chemical components of Fu-Zi Decoction, 551 drug targets, and 521 disease targets were collected. Functional enrichment analysis revealed that it was mainly involved in biological processes, such as negative regulation of cell death, oxidative stress, and G protein-coupled receptor regulation. KEGG enrichment findings mainly involved fluid shear stress and atherosclerosis, IL-17 signaling pathway, and so on. The results of molecular docking showed that benzoylaconitine, aconitine, mesaconitine, paeoniflorin, and atractylodes Ⅲ all had a strong affinity with the core target CXCL8, suggesting that Fu-Zi Decoction could negatively regulate cell apoptosis and oxidative stress through fluid shear stress and atherosclerosis, IL-17 signaling pathway, and so on. Collectively, our data showed that Fu-Zi Decoction had a good effect on the treatment of CHF.
基金Financial support from the National Natural Science Foundation of China(NNSFC,Nos.81630094 and 30825044)
文摘Four structurally unprecedented aconitane-type C_（19）-diterpenoid alkaloid glycosides with isomeric arabinosyls, named aconicarmichosides A–D（1–4）, were isolated from an aqueous extract of ＂fu zi＂, the lateral roots of Aconitum carmichaelii. Their structures were determined as neoline 14-O-a-and 14-O-b-L-arabinopyranosides（1 and 2） and 14-O-a-and 14-O-b-L-arabinofuranosides（3 and 4）, by spectroscopic and chemical methods including 2D NMR experiments and acid hydrolysis. Compounds 1–4 represent the first examples of glycosidic diterpenoid alkaloids.
