Since whole-genome sequencing of many crops has been achieved,crop functional genomics studies have stepped into the big-data and high-throughput era.However,acquisition of large-scale phenotypic data has become one o...Since whole-genome sequencing of many crops has been achieved,crop functional genomics studies have stepped into the big-data and high-throughput era.However,acquisition of large-scale phenotypic data has become one of the major bottlenecks hindering crop breeding and functional genomics studies.Nevertheless,recent technological advances provide us potential solutions to relieve this bottleneck and to explore advanced methods for large-scale phenotyping data acquisition and processing in the coming years.In this article,we review the major progress on high-throughput phenotyping in controlled environments and field conditions as well as its use for post-harvest yield and quality assessment in the past decades.We then discuss the latest multi-omics research combining high-throughput phenotyping with genetic studies.Finally,we propose some conceptual challenges and provide our perspectives on how to bridge the phenotype-genotype gap.It is no doubt that accurate high-throughput phenotyping will accelerate plant genetic improvements and promote the next green revolution in crop breeding.展开更多
研究静电场对大菱鲆贮藏品质的影响,比较不同贮藏条件下大菱鲆的菌落总数、假单胞菌、总挥发性盐基氮(total volatile base nitrogen,TVB-N)值和硫代巴比妥酸(thiobarbituric acid,TBA)值。采用电子鼻采集其气体指纹信息,通过离子气相...研究静电场对大菱鲆贮藏品质的影响,比较不同贮藏条件下大菱鲆的菌落总数、假单胞菌、总挥发性盐基氮(total volatile base nitrogen,TVB-N)值和硫代巴比妥酸(thiobarbituric acid,TBA)值。采用电子鼻采集其气体指纹信息,通过离子气相迁移谱对不同处理方式大菱鲆的挥发性物质进行分析检测。结果表明:静电场能够改善贮藏期间大菱鲆的品质,有效抑制菌落总数和优势腐败菌的生长及减少TVB-N值和TBA值的增加,在一定程度上抑制了鱼肉内脂肪氧化,具有较好的保鲜效果。采用主成分分析、线性判别分析方法可较好区分不同贮藏时间及不同处理方式的大菱鲆品质。根据气相离子迁移谱采集的指纹图谱,利用热图聚类分析可区分不同贮藏时间内及不同处理方式下大菱鲆挥发性物质。气相离子迁移谱与电子鼻具有快速、准确、无损的特点,可对大菱鲆新鲜度品质进行快速检测可行。展开更多
[Objective] The objective of this study was to explore the long-term effect of Fenlong cultivation for one time. [Method] The physical and chemical properties of soil and rice yield and quality were investigated and d...[Objective] The objective of this study was to explore the long-term effect of Fenlong cultivation for one time. [Method] The physical and chemical properties of soil and rice yield and quality were investigated and determined by measuring the soil at the depth of 23 cm in the paddy field with Fenlong cultivation and 16 cm of control （conventional farming） for 7 years. [Result] Compared with the control, the average yield of early rice of the Fenlong cultivation increased by 1 972.5 kg/hm2 of the first year, an increase of 23.87%, and milled rice and protein increased by 15.95% and 14.61%, while the increase rates of organic matter, available nitrogen, available phosphorus and available potassium per hectare were 74.58%, 67.01%, 104.41% and 129.62%, respectively. In the 7th year after Fenlong cultivation, the yield increased by 234 kg/hm2 from the control, an increase of 3.21%, and milled rice and protein increased by 0.5% and 0.14%, while the increase rates of organic matter, available phosphorus and available potassium per hectare were 745.8%, 62.635 and 73.37%, respectively, and the increase rates of total nitrogen, total phosphorus and total potassium were 46.03%, 50.58% and 36.65%, respectively. During the 7 years after Fenlong cultivation, the yield increased by 3.21-23.87%, and its average net income per season increased by 1 843.61 Yuan/hm2 with a gain of 18.03%. [Conclusion] At the 7th year after Fenlong cultivation, the paddy field still had the furrow remain at a depth of 22 cm, which was 46.67% thicker than the traditional one. The yield and quality of paddy rice increased for 7 consecutive vears showed that Fenlon.q cultivation was highly feasible for paddy field.展开更多
基金the National Key Research and Development Program of China(2016YFD0100101-18,2016YFD0100103)the National Natural Science Foundation of China(31770397,21800305)+2 种基金the Fundamental Research Funds for the Central Universities(2662017PY058,2662017QD044)UK-China grant BBSRC(grant no.BB/R02118X/1)the National Institute of Food and Agriculture,U.S.Department of Agriculture,Hatch project(ALA014-1-16016).
文摘Since whole-genome sequencing of many crops has been achieved,crop functional genomics studies have stepped into the big-data and high-throughput era.However,acquisition of large-scale phenotypic data has become one of the major bottlenecks hindering crop breeding and functional genomics studies.Nevertheless,recent technological advances provide us potential solutions to relieve this bottleneck and to explore advanced methods for large-scale phenotyping data acquisition and processing in the coming years.In this article,we review the major progress on high-throughput phenotyping in controlled environments and field conditions as well as its use for post-harvest yield and quality assessment in the past decades.We then discuss the latest multi-omics research combining high-throughput phenotyping with genetic studies.Finally,we propose some conceptual challenges and provide our perspectives on how to bridge the phenotype-genotype gap.It is no doubt that accurate high-throughput phenotyping will accelerate plant genetic improvements and promote the next green revolution in crop breeding.
文摘研究静电场对大菱鲆贮藏品质的影响,比较不同贮藏条件下大菱鲆的菌落总数、假单胞菌、总挥发性盐基氮(total volatile base nitrogen,TVB-N)值和硫代巴比妥酸(thiobarbituric acid,TBA)值。采用电子鼻采集其气体指纹信息,通过离子气相迁移谱对不同处理方式大菱鲆的挥发性物质进行分析检测。结果表明:静电场能够改善贮藏期间大菱鲆的品质,有效抑制菌落总数和优势腐败菌的生长及减少TVB-N值和TBA值的增加,在一定程度上抑制了鱼肉内脂肪氧化,具有较好的保鲜效果。采用主成分分析、线性判别分析方法可较好区分不同贮藏时间及不同处理方式的大菱鲆品质。根据气相离子迁移谱采集的指纹图谱,利用热图聚类分析可区分不同贮藏时间内及不同处理方式下大菱鲆挥发性物质。气相离子迁移谱与电子鼻具有快速、准确、无损的特点,可对大菱鲆新鲜度品质进行快速检测可行。
文摘[Objective] The objective of this study was to explore the long-term effect of Fenlong cultivation for one time. [Method] The physical and chemical properties of soil and rice yield and quality were investigated and determined by measuring the soil at the depth of 23 cm in the paddy field with Fenlong cultivation and 16 cm of control （conventional farming） for 7 years. [Result] Compared with the control, the average yield of early rice of the Fenlong cultivation increased by 1 972.5 kg/hm2 of the first year, an increase of 23.87%, and milled rice and protein increased by 15.95% and 14.61%, while the increase rates of organic matter, available nitrogen, available phosphorus and available potassium per hectare were 74.58%, 67.01%, 104.41% and 129.62%, respectively. In the 7th year after Fenlong cultivation, the yield increased by 234 kg/hm2 from the control, an increase of 3.21%, and milled rice and protein increased by 0.5% and 0.14%, while the increase rates of organic matter, available phosphorus and available potassium per hectare were 745.8%, 62.635 and 73.37%, respectively, and the increase rates of total nitrogen, total phosphorus and total potassium were 46.03%, 50.58% and 36.65%, respectively. During the 7 years after Fenlong cultivation, the yield increased by 3.21-23.87%, and its average net income per season increased by 1 843.61 Yuan/hm2 with a gain of 18.03%. [Conclusion] At the 7th year after Fenlong cultivation, the paddy field still had the furrow remain at a depth of 22 cm, which was 46.67% thicker than the traditional one. The yield and quality of paddy rice increased for 7 consecutive vears showed that Fenlon.q cultivation was highly feasible for paddy field.
