Objective:To measure the effect of doxycycline on inflammatory marker[IL-6,TNF-α,ferritin and C reactive protein(CRP)]levels in patients with dengue infection.Methods:A single-centre,open-label,parallel-group randomi...Objective:To measure the effect of doxycycline on inflammatory marker[IL-6,TNF-α,ferritin and C reactive protein(CRP)]levels in patients with dengue infection.Methods:A single-centre,open-label,parallel-group randomized controlled trial was done in PGIMER Chandigarh from June 2021 to October 2022.Patients were randomized using a simple randomization process into two groups:the doxycycline treatment group(n=35)and the control group(n=34).Patients in the treatment group were given oral doxycycline 100 mg twice daily for five days along with standard treatment,whereas patients in the control group received only standard treatment.The objective was to measure the effect of doxycycline on inflammatory markers in dengue infection.Results:On comparative analysis at day 5,there was a statistically significant reduction in the median values of ferritin and CRP in cases compared to the control group(ferritin:P=0.006 and CRP:P=0.006).No significant reduction was noted in the levels of IL-6 and TNF-α.Conclusions:Doxycycline treatment led to a reduction of inflammatory markers in dengue infection.展开更多
Objective To establish and evaluate a protein serum ferritin （SF） and soluble transferrin receptor microarray method for combined measurement of （sTfR）. Methods Microarrayer was used to print both anti-SF antibodi...Objective To establish and evaluate a protein serum ferritin （SF） and soluble transferrin receptor microarray method for combined measurement of （sTfR）. Methods Microarrayer was used to print both anti-SF antibodies I and anti-sTfR antibodies I on each protein microarray. Anti-SF antibodies II and anti-sTfR antibodies II were used as detection antibodies and goat antibodies coupled to Cy3 were used as antibodies Ill. The detection conditions of the quantitative analysis method for simultaneous measurement of SF and sTfR with protein microarray were optimized and evaluated. The protein microarray was compared with commercially available traditional tests with 26 serum samples. Results By comparison experiment, mouse monoclonal antibodies were chosen as the probes and contact printing was chosen as the printing method. The concentrations of SF and sTfR probes were 0.5 mg/mL and 0.5 mg/mL respectively, while those of SF and sTfR detection antibodies were 5 μg/mL and 0.36 μg/mL respectively. Intra- and inter-assay variability was between 3.26% and 18.38% for all tests. The regression coefficients comparing protein microarray with traditional test assays were better than 0.81 for SF and sTfR. Conclusion The present study has established a protein microarray method for combined measurement of SF and sTfR.展开更多
Ferritin stores and releases iron ions in mammals.It is globally important as a drug nanocarrier.This is because of its unique hollow-spherical structure,desirable stability and biological properties.Novel drug-loadin...Ferritin stores and releases iron ions in mammals.It is globally important as a drug nanocarrier.This is because of its unique hollow-spherical structure,desirable stability and biological properties.Novel drug-loading approaches plus various functionalization approaches have been developed to improve ferritin in response to differing demands in disease treatments.Here,we critically review ferritin drug delivery and evaluate its diverse drug-loading and functionalization approaches,we:(1)Introduce basic structural and property information related to ferritin as a drug nanocarrier;(2)Contrast in detail the different means to load drugs and the selection of drug loading means;(3)Discuss multiple ferritin functionalization approaches,together with related advantages and potential risks;and,(4)Compare ferritin with alternative,commonly-used drug nanocarriers.We conclude that despite that no drugs based on ferritin are commercially available,the market potential for it is significant,and evaluate future research directions.Findings from this work will be of immediate benefit and interest to a wide range of researchers and manufacturers for drug delivery using ferritin.展开更多
目的通过原核表达人重链铁蛋白基因(recombinant human H-chain ferritin,rHF),为新型纳米疫苗的研制提供新方法和手段。方法将人源性铁蛋白氨基酸序列按照大肠杆菌常用密码子进行优化,合成重组人重链铁蛋白(rHF)基因并克隆至原核表达载...目的通过原核表达人重链铁蛋白基因(recombinant human H-chain ferritin,rHF),为新型纳米疫苗的研制提供新方法和手段。方法将人源性铁蛋白氨基酸序列按照大肠杆菌常用密码子进行优化,合成重组人重链铁蛋白(rHF)基因并克隆至原核表达载体pET-32a。通过双酶切、测序鉴定阳性重组质粒,将阳性质粒转入感受态细胞BL21(DE3),再经过异丙基-β-D-硫代半乳糖苷(IPTG)诱导表达。通过镍柱纯化重组蛋白。结果成功构建并表达纯化重组蛋白,经SDS-PAGE和Western blot鉴定蛋白产物,利用电子透射显微镜观察重组蛋白构型。重组铁蛋白以可溶性形式存在于蛋白上清,并具有生物学活性。且能够胞外自组装成具有12-20nm狭窄尺寸分布的纳米笼结构。结论采用大肠埃希菌表达系统可表达可溶性rHF蛋白并具有纳米粒结构,为新型纳米疫苗的研制以及疾病诊治提供了理论基础。展开更多
Accumulation of lipid-laden macrophages(foam cells)is characteristic of atherosclerosis developme nt in the arterial walls.Ferritin nano cages have been found to passively accumulate in the atherosclerotic plaque.Ferr...Accumulation of lipid-laden macrophages(foam cells)is characteristic of atherosclerosis developme nt in the arterial walls.Ferritin nano cages have been found to passively accumulate in the atherosclerotic plaque.Ferritin has been actively investigated as a carrier for contrast agents in atherosclerosis diag no sis.We dem on strate the pote ntial of ferritin as a carrier for therapeutic molecules to mediate cholesterol reducti on from foam cells.Cyclodextrin molecules are chemically conjugated to the ferritin anocages surface or encapsulated within the nanocages using metal co-loadi ng methods.The cyclodextrin-conjugated ferriti n has nano molar affinity to cholesterol molecules.Treatment of foam cells with the conjugates shows decreased levels of intracellular accumulated cholesterol.The preferential localization of ferritin to foam cells is due to transferrin receptor-mediated endocytosis process.These findings show that ferritin nanocages as carriers localize cyclodextrin molecules to foam cells which mediate intracellular cholesterol reductio n,thus highlight!ng its potential use as a therapeutic agent.展开更多
In a recent article on World J Clin Cases 2019;7:3859-3865,Sun et al reported a case of 36-year-old female with macrophage activity syndrome as an onset of systemic lupus erythematosus.Although this is a very interest...In a recent article on World J Clin Cases 2019;7:3859-3865,Sun et al reported a case of 36-year-old female with macrophage activity syndrome as an onset of systemic lupus erythematosus.Although this is a very interesting case,some concerns still need to be addressed.First,the patient had an extremely elevated serum ferritin but a normal C-reactive protein level,which was unparallel with the inflammatory condition before she received any treatments.Second,the diagnosis of systemic lupus erythematosus seemed to be insufficient according to the patient’s medical information presented,most of which were not specific to lupus but could be explained by macrophage activity syndrome.Hence,more medical information on the patient should be provided,and a profound discussion needs to be addressed.展开更多
文摘Objective:To measure the effect of doxycycline on inflammatory marker[IL-6,TNF-α,ferritin and C reactive protein(CRP)]levels in patients with dengue infection.Methods:A single-centre,open-label,parallel-group randomized controlled trial was done in PGIMER Chandigarh from June 2021 to October 2022.Patients were randomized using a simple randomization process into two groups:the doxycycline treatment group(n=35)and the control group(n=34).Patients in the treatment group were given oral doxycycline 100 mg twice daily for five days along with standard treatment,whereas patients in the control group received only standard treatment.The objective was to measure the effect of doxycycline on inflammatory markers in dengue infection.Results:On comparative analysis at day 5,there was a statistically significant reduction in the median values of ferritin and CRP in cases compared to the control group(ferritin:P=0.006 and CRP:P=0.006).No significant reduction was noted in the levels of IL-6 and TNF-α.Conclusions:Doxycycline treatment led to a reduction of inflammatory markers in dengue infection.
基金funded by the 863 Program entitled as"The research and exploration of nutrition fortified food for improving growth and development(2010AA023004)"performed by the Trace Elements Nutrition Key Laboratory of the Ministry of Health
文摘Objective To establish and evaluate a protein serum ferritin （SF） and soluble transferrin receptor microarray method for combined measurement of （sTfR）. Methods Microarrayer was used to print both anti-SF antibodies I and anti-sTfR antibodies I on each protein microarray. Anti-SF antibodies II and anti-sTfR antibodies II were used as detection antibodies and goat antibodies coupled to Cy3 were used as antibodies Ill. The detection conditions of the quantitative analysis method for simultaneous measurement of SF and sTfR with protein microarray were optimized and evaluated. The protein microarray was compared with commercially available traditional tests with 26 serum samples. Results By comparison experiment, mouse monoclonal antibodies were chosen as the probes and contact printing was chosen as the printing method. The concentrations of SF and sTfR probes were 0.5 mg/mL and 0.5 mg/mL respectively, while those of SF and sTfR detection antibodies were 5 μg/mL and 0.36 μg/mL respectively. Intra- and inter-assay variability was between 3.26% and 18.38% for all tests. The regression coefficients comparing protein microarray with traditional test assays were better than 0.81 for SF and sTfR. Conclusion The present study has established a protein microarray method for combined measurement of SF and sTfR.
基金funded by joint PhD Scholarship Scheme of the University of Adelaide and Institute of Process EngineeringChinese Academy of Sciences+1 种基金the National Natural Science Foundation of China(Grant No.21576267)Beijing Natural Science Foundation(Grant Number 2162041).
文摘Ferritin stores and releases iron ions in mammals.It is globally important as a drug nanocarrier.This is because of its unique hollow-spherical structure,desirable stability and biological properties.Novel drug-loading approaches plus various functionalization approaches have been developed to improve ferritin in response to differing demands in disease treatments.Here,we critically review ferritin drug delivery and evaluate its diverse drug-loading and functionalization approaches,we:(1)Introduce basic structural and property information related to ferritin as a drug nanocarrier;(2)Contrast in detail the different means to load drugs and the selection of drug loading means;(3)Discuss multiple ferritin functionalization approaches,together with related advantages and potential risks;and,(4)Compare ferritin with alternative,commonly-used drug nanocarriers.We conclude that despite that no drugs based on ferritin are commercially available,the market potential for it is significant,and evaluate future research directions.Findings from this work will be of immediate benefit and interest to a wide range of researchers and manufacturers for drug delivery using ferritin.
文摘目的通过原核表达人重链铁蛋白基因(recombinant human H-chain ferritin,rHF),为新型纳米疫苗的研制提供新方法和手段。方法将人源性铁蛋白氨基酸序列按照大肠杆菌常用密码子进行优化,合成重组人重链铁蛋白(rHF)基因并克隆至原核表达载体pET-32a。通过双酶切、测序鉴定阳性重组质粒,将阳性质粒转入感受态细胞BL21(DE3),再经过异丙基-β-D-硫代半乳糖苷(IPTG)诱导表达。通过镍柱纯化重组蛋白。结果成功构建并表达纯化重组蛋白,经SDS-PAGE和Western blot鉴定蛋白产物,利用电子透射显微镜观察重组蛋白构型。重组铁蛋白以可溶性形式存在于蛋白上清,并具有生物学活性。且能够胞外自组装成具有12-20nm狭窄尺寸分布的纳米笼结构。结论采用大肠埃希菌表达系统可表达可溶性rHF蛋白并具有纳米粒结构,为新型纳米疫苗的研制以及疾病诊治提供了理论基础。
文摘Accumulation of lipid-laden macrophages(foam cells)is characteristic of atherosclerosis developme nt in the arterial walls.Ferritin nano cages have been found to passively accumulate in the atherosclerotic plaque.Ferritin has been actively investigated as a carrier for contrast agents in atherosclerosis diag no sis.We dem on strate the pote ntial of ferritin as a carrier for therapeutic molecules to mediate cholesterol reducti on from foam cells.Cyclodextrin molecules are chemically conjugated to the ferritin anocages surface or encapsulated within the nanocages using metal co-loadi ng methods.The cyclodextrin-conjugated ferriti n has nano molar affinity to cholesterol molecules.Treatment of foam cells with the conjugates shows decreased levels of intracellular accumulated cholesterol.The preferential localization of ferritin to foam cells is due to transferrin receptor-mediated endocytosis process.These findings show that ferritin nanocages as carriers localize cyclodextrin molecules to foam cells which mediate intracellular cholesterol reductio n,thus highlight!ng its potential use as a therapeutic agent.
文摘In a recent article on World J Clin Cases 2019;7:3859-3865,Sun et al reported a case of 36-year-old female with macrophage activity syndrome as an onset of systemic lupus erythematosus.Although this is a very interesting case,some concerns still need to be addressed.First,the patient had an extremely elevated serum ferritin but a normal C-reactive protein level,which was unparallel with the inflammatory condition before she received any treatments.Second,the diagnosis of systemic lupus erythematosus seemed to be insufficient according to the patient’s medical information presented,most of which were not specific to lupus but could be explained by macrophage activity syndrome.Hence,more medical information on the patient should be provided,and a profound discussion needs to be addressed.
