报道了中国希瓦氏菌D14 T 的Fe(Ⅲ)还原特性,研究了溶氧浓度、光照强度、温度、pH等条件对菌株Fe(Ⅲ)还原的影响。结果发现,随着培养基中Fe(Ⅲ)浓度的提高,菌株D14 T 的Fe(Ⅲ)还原速率相应降低;氧气和光照对Fe(Ⅲ)还原有一定的抑制作用...报道了中国希瓦氏菌D14 T 的Fe(Ⅲ)还原特性,研究了溶氧浓度、光照强度、温度、pH等条件对菌株Fe(Ⅲ)还原的影响。结果发现,随着培养基中Fe(Ⅲ)浓度的提高,菌株D14 T 的Fe(Ⅲ)还原速率相应降低;氧气和光照对Fe(Ⅲ)还原有一定的抑制作用;菌株还原Fe(Ⅲ)的最适反应温度为37℃;在反应起始pH6 0 - 10 0的条件下菌株可进行Fe(Ⅲ)还原。对不同形态Fe(Ⅲ)还原特性的研究结果表明,Fe(Ⅲ)的溶解度越高越有利于还原反应的进行。采用SDS和OGP这两种蛋白变性剂对Fe(Ⅲ)还原蛋白进行初步定位的结果表明,参与Fe(Ⅲ)还原的蛋白主要位于细胞可溶性外周蛋白。在同时含有偶氮染料和Fe(Ⅲ)的条件下,菌株D14 T 的偶氮染料脱色率和Fe(Ⅲ)展开更多
Microbial Fe(Ⅲ)reduction is a significant driving force for the biogeochemical cycles of C,O,P,S,N,and dominates the natural bio-purification of contaminants in groundwater(e.g.,petroleum hydrocarbons,chlorinated eth...Microbial Fe(Ⅲ)reduction is a significant driving force for the biogeochemical cycles of C,O,P,S,N,and dominates the natural bio-purification of contaminants in groundwater(e.g.,petroleum hydrocarbons,chlorinated ethane,and chromium).In this review,the mechanisms and environmental significance of Fe(Ⅲ)(hydro)oxides bioreduction are summarized.Compared with crystalline Fe(Ⅲ)(hydro)oxides,amorphous Fe(m)(hydro)oxides are more bioavailable.Ligand and electron shuttle both play an important role in microbial Fe(Ⅲ)reduction.The restrictive factors of Fe(Ⅲ)(hydro)oxides bioreduction should be further investigated to reveal the characteristics and mechanisms of the process.It will improve the bioavailability of crystalline Fe(Ⅲ)(hydro)oxides and accelerate the anaerobic oxidation efficiency of the reduction state pollutants.Furthermore,the approach to extract,culture,and incubate the functional Fe(Ⅲ)reducing bacteria from actual complicated environment,and applying it to the bioremediation of organic,ammonia,and heavy metals contaminated groundwater will become a research topic in the future.There are a broad application prospects of Fe(Ⅲ)(hydro)oxides bioreduction to groundwater bioremediation,which includes the in situ injection and permeable reactive barriers and the innovative Kariz wells system.The study provides an important reference for the treatment of reduced pollutants in contaminated groundwater.展开更多
文摘报道了中国希瓦氏菌D14 T 的Fe(Ⅲ)还原特性,研究了溶氧浓度、光照强度、温度、pH等条件对菌株Fe(Ⅲ)还原的影响。结果发现,随着培养基中Fe(Ⅲ)浓度的提高,菌株D14 T 的Fe(Ⅲ)还原速率相应降低;氧气和光照对Fe(Ⅲ)还原有一定的抑制作用;菌株还原Fe(Ⅲ)的最适反应温度为37℃;在反应起始pH6 0 - 10 0的条件下菌株可进行Fe(Ⅲ)还原。对不同形态Fe(Ⅲ)还原特性的研究结果表明,Fe(Ⅲ)的溶解度越高越有利于还原反应的进行。采用SDS和OGP这两种蛋白变性剂对Fe(Ⅲ)还原蛋白进行初步定位的结果表明,参与Fe(Ⅲ)还原的蛋白主要位于细胞可溶性外周蛋白。在同时含有偶氮染料和Fe(Ⅲ)的条件下,菌株D14 T 的偶氮染料脱色率和Fe(Ⅲ)
基金This work was supported by the National Natural Science Foundation of China(Grant No.21606214)the Water Pollution Control and Control of Major National Science and Technology Projects in China(No.2018ZX07109-003)。
文摘Microbial Fe(Ⅲ)reduction is a significant driving force for the biogeochemical cycles of C,O,P,S,N,and dominates the natural bio-purification of contaminants in groundwater(e.g.,petroleum hydrocarbons,chlorinated ethane,and chromium).In this review,the mechanisms and environmental significance of Fe(Ⅲ)(hydro)oxides bioreduction are summarized.Compared with crystalline Fe(Ⅲ)(hydro)oxides,amorphous Fe(m)(hydro)oxides are more bioavailable.Ligand and electron shuttle both play an important role in microbial Fe(Ⅲ)reduction.The restrictive factors of Fe(Ⅲ)(hydro)oxides bioreduction should be further investigated to reveal the characteristics and mechanisms of the process.It will improve the bioavailability of crystalline Fe(Ⅲ)(hydro)oxides and accelerate the anaerobic oxidation efficiency of the reduction state pollutants.Furthermore,the approach to extract,culture,and incubate the functional Fe(Ⅲ)reducing bacteria from actual complicated environment,and applying it to the bioremediation of organic,ammonia,and heavy metals contaminated groundwater will become a research topic in the future.There are a broad application prospects of Fe(Ⅲ)(hydro)oxides bioreduction to groundwater bioremediation,which includes the in situ injection and permeable reactive barriers and the innovative Kariz wells system.The study provides an important reference for the treatment of reduced pollutants in contaminated groundwater.
