Background:Non-alcoholic fatty liver disease(NAFLD)is the most common chronic liver diseaseworldwide,ranging from simple steatosis to non-alcoholic steatohepatitis(NASH)and fibrosis.Possiblereasons for the NAFLD epide...Background:Non-alcoholic fatty liver disease(NAFLD)is the most common chronic liver diseaseworldwide,ranging from simple steatosis to non-alcoholic steatohepatitis(NASH)and fibrosis.Possiblereasons for the NAFLD epidemic in industrialized countries are the high intake of pro-inflammatory n-6polyunsaturated fatty acids(n-6 PUFAs)and low consumption of healthy n-3 PUFAs.Due to their antiinflammatoryproperties,n-3 PUFAs may have the potential to alleviate chronic liver disease.Herein,weexamined the therapeutic effect of increased n-3 PUFA tissue levels in fat-1 transgenic mice on progressiveNASH.Methods:Disease was induced in mice by streptozotocin and high fat diet(STZ/HFD)resulting in NASH.NAFLD in 6 and 8 weeks old wild type and fat-1 transgenic STZ/HFD treated mice was analyzed.Unlikeall other mammals,fat-1 transgenic mice ubiquitously express an n-3 fatty acid desaturase,which converts n-6to n-3 PUFAs,leading to increased n-3 and decreased n-6 PUFA tissue contents.Results:Liver damage,NAFLD activity score(NAS),hepatic lipid accumulation and inflammation weresignificantly reduced in fat-1 transgenic STZ/HFD treated mice in the early(6 weeks)but not late(8 weeks)phase of NASH.Simultaneously,mRNA expression of genes involved in fatty acid uptake and storage(Cd36and Plin3,respectively)was significantly down-regulated in 6 week old but not 8 week old fat-1 transgenicSTZ/HFD treated mice.Conclusions:Endogenously elevated n-3 PUFA levels in fat-1 transgenic mice transiently delay the onsetof STZ/HFD induced NASH but failed to efficiently protect from NASH development.展开更多
Objective: To study the change of Fat-1 gene expression in endometrial cancer and its regulating effect on the growth of cancer cells. Methods: A total of 150 patients with endometrial cancer who underwent surgical re...Objective: To study the change of Fat-1 gene expression in endometrial cancer and its regulating effect on the growth of cancer cells. Methods: A total of 150 patients with endometrial cancer who underwent surgical resection in Xijing Hospital between August 2016 and August 2017 were selected as the malignant group of the study to collect the endometrial cancer tissues, 80 patients with benign lesions who underwent total hysterectomy in Xijing Hospital during the same period were selected as the control group of the study to collect normal endometrial tissues, and the mRNA expression levels of Fat-1 gene, tumor suppressor genes, proliferation genes and angiogenesis genes were determined. Results: Fat-1, PTEN, p16, BRCA1, Bid and Caspase-3 mRNA expression in endometrial cancer tissues of malignant group were significantly lower than those of control group whereas SRPX2, TET1, CyclinD1, HMGB1, Livin, Septin9, β-arrestin2, HIF-1α and VEGF mRNA expression in endometrial cancer tissues of malignant group were significantly higher than those of control group;Pearson analysis showed that Fat-1 mRNA expression in endometrial cancer tissues was positively correlated with PTEN, p16, BRCA1, Bid and Caspase-3 mRNA expression, and negatively correlated with SRPX2, TET1, CyclinD1, HMGB1, Livin, Septin9, β-arrestin2, HIF-1α and VEGF mRNA expression. Conclusion: The low expression of Fat-1 gene in endometrial cancer promotes the growth of cancer cells.展开更多
基金This work was supported by the Deutsche Forschungsgemeinschaft,Bonn-Bad Godesberg,Germany(AB 453/2-1).
文摘Background:Non-alcoholic fatty liver disease(NAFLD)is the most common chronic liver diseaseworldwide,ranging from simple steatosis to non-alcoholic steatohepatitis(NASH)and fibrosis.Possiblereasons for the NAFLD epidemic in industrialized countries are the high intake of pro-inflammatory n-6polyunsaturated fatty acids(n-6 PUFAs)and low consumption of healthy n-3 PUFAs.Due to their antiinflammatoryproperties,n-3 PUFAs may have the potential to alleviate chronic liver disease.Herein,weexamined the therapeutic effect of increased n-3 PUFA tissue levels in fat-1 transgenic mice on progressiveNASH.Methods:Disease was induced in mice by streptozotocin and high fat diet(STZ/HFD)resulting in NASH.NAFLD in 6 and 8 weeks old wild type and fat-1 transgenic STZ/HFD treated mice was analyzed.Unlikeall other mammals,fat-1 transgenic mice ubiquitously express an n-3 fatty acid desaturase,which converts n-6to n-3 PUFAs,leading to increased n-3 and decreased n-6 PUFA tissue contents.Results:Liver damage,NAFLD activity score(NAS),hepatic lipid accumulation and inflammation weresignificantly reduced in fat-1 transgenic STZ/HFD treated mice in the early(6 weeks)but not late(8 weeks)phase of NASH.Simultaneously,mRNA expression of genes involved in fatty acid uptake and storage(Cd36and Plin3,respectively)was significantly down-regulated in 6 week old but not 8 week old fat-1 transgenicSTZ/HFD treated mice.Conclusions:Endogenously elevated n-3 PUFA levels in fat-1 transgenic mice transiently delay the onsetof STZ/HFD induced NASH but failed to efficiently protect from NASH development.
基金Natural Science Foundation of China,No:8167102057.
文摘Objective: To study the change of Fat-1 gene expression in endometrial cancer and its regulating effect on the growth of cancer cells. Methods: A total of 150 patients with endometrial cancer who underwent surgical resection in Xijing Hospital between August 2016 and August 2017 were selected as the malignant group of the study to collect the endometrial cancer tissues, 80 patients with benign lesions who underwent total hysterectomy in Xijing Hospital during the same period were selected as the control group of the study to collect normal endometrial tissues, and the mRNA expression levels of Fat-1 gene, tumor suppressor genes, proliferation genes and angiogenesis genes were determined. Results: Fat-1, PTEN, p16, BRCA1, Bid and Caspase-3 mRNA expression in endometrial cancer tissues of malignant group were significantly lower than those of control group whereas SRPX2, TET1, CyclinD1, HMGB1, Livin, Septin9, β-arrestin2, HIF-1α and VEGF mRNA expression in endometrial cancer tissues of malignant group were significantly higher than those of control group;Pearson analysis showed that Fat-1 mRNA expression in endometrial cancer tissues was positively correlated with PTEN, p16, BRCA1, Bid and Caspase-3 mRNA expression, and negatively correlated with SRPX2, TET1, CyclinD1, HMGB1, Livin, Septin9, β-arrestin2, HIF-1α and VEGF mRNA expression. Conclusion: The low expression of Fat-1 gene in endometrial cancer promotes the growth of cancer cells.