[目的]探讨鳖甲寡肽I-C-F-6对乙醇诱导的小鼠慢性肝损伤的预防作用,并分析其可能的保护机制。[方法]将90只ICR小鼠随机分为正常对照组、模型组、寡肽低剂量组(0.12 mg/kg)、寡肽高剂量组(0.24 mg/kg)、阳性药组(240.00 mg/kg)。正常对...[目的]探讨鳖甲寡肽I-C-F-6对乙醇诱导的小鼠慢性肝损伤的预防作用,并分析其可能的保护机制。[方法]将90只ICR小鼠随机分为正常对照组、模型组、寡肽低剂量组(0.12 mg/kg)、寡肽高剂量组(0.24 mg/kg)、阳性药组(240.00 mg/kg)。正常对照组和模型组均皮下注射生理盐水(0.12 m L/kg);寡肽低剂量组和寡肽高剂量组分别皮下注射寡肽的生理盐水溶液(0.12 m L/kg);阳性药组灌胃还原型谷胱甘肽生理盐水溶液(0.12 m L/kg),同时除正常对照组外,其余4组给予灌胃40%乙醇(0.12 m L/kg),每日1次,连续16周。16周后,末次给乙醇12 h后处死小鼠采集血液样品和肝组织样品,测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和碱性磷酸酶(AKP)的活力,肝组织超氧化物歧化酶(SOD)活力、丙二醛含量(MDA),单胺氧化酶(MAO)的活力,并进行病理组织学检查。[结果]鳖甲寡肽I-C-F-6使灌胃乙醇后小鼠体内ALT、AST、MDA、MAO、AKP的含量显著降低(P<0.05),肝组织匀浆中SOD的活力明显升高(P<0.05),肝脏病理损伤减轻。[结论]鳖甲寡肽I-C-F-6对小鼠慢性酒精性肝损伤具有一定的预防保护作用,其机制可能与抑制脂质过氧化,清除自由基,升高SOD的活力有关。展开更多
应用 A 株、F_(48)E_8株新城疫病毒对不同日龄鹌鹑人工发病试验,结果表明:不同日龄鹌鹑对两毒株均有较强的易感性;随日龄增加到成年鹌鹑时则易感性降低。A 株和 F_(48)E_8株病毒所致的病变在各日龄基本相似,但 A 株比 F_(48)E_8株病毒...应用 A 株、F_(48)E_8株新城疫病毒对不同日龄鹌鹑人工发病试验,结果表明:不同日龄鹌鹑对两毒株均有较强的易感性;随日龄增加到成年鹌鹑时则易感性降低。A 株和 F_(48)E_8株病毒所致的病变在各日龄基本相似,但 A 株比 F_(48)E_8株病毒对神经系统的损伤为重。展开更多
Enteric disorders in pigs are related to the fimbriae F4 (K88), F5 (K99), F6 (987P), F41 and F18 of enterotoxigenic Escherichia coli (ETEC). Immunization of sows with adhesins is important to stimulate the production ...Enteric disorders in pigs are related to the fimbriae F4 (K88), F5 (K99), F6 (987P), F41 and F18 of enterotoxigenic Escherichia coli (ETEC). Immunization of sows with adhesins is important to stimulate the production of antibodies and the consequent transfer of these to the piglets via colostrum to prevent diarrhea during the neonate period and after weaning. The objective of this study was to evaluate the immune response of the sows immunized with recombinant ETEC proteins (F4, F5, F6, F18 and F41). The immune response of the sows immunized with the recombinant proteins was compared with a commercial vaccine containing ETEC bacterins. The study was performed on a commercial farm and included nine pregnant sows divided into three groups: G1 was vaccinated with recombinant proteins (n = 3);G2 was vaccinated with the commercial vaccine (n = 3);and G3 was vaccinated with sterile buffered saline (PBS) (n = 3). All the sows were fed a balanced diet without antibiotics and water ad libitum. The recombinant fimbriae stimulated the specific humoral immune response of the immunized sows. There was a statistically significant increase in the levels of antibodies to the fimbriae F4 (K88), F5 (K99), F6 (987P) and F18 in the sows vaccinated with the recombinant proteins compared with the control group. The colostrum IgG titers for all fimbriae in all the immunized sows were significantly increased compared to the control group. Additionally, all the piglets exhibited significantly increased antibody levels relative to all fimbriae when compared with those in the unimmunized control group, demonstrating successful antibody transfer via colostrum of the sows to the piglets.展开更多
文摘[目的]探讨鳖甲寡肽I-C-F-6对乙醇诱导的小鼠慢性肝损伤的预防作用,并分析其可能的保护机制。[方法]将90只ICR小鼠随机分为正常对照组、模型组、寡肽低剂量组(0.12 mg/kg)、寡肽高剂量组(0.24 mg/kg)、阳性药组(240.00 mg/kg)。正常对照组和模型组均皮下注射生理盐水(0.12 m L/kg);寡肽低剂量组和寡肽高剂量组分别皮下注射寡肽的生理盐水溶液(0.12 m L/kg);阳性药组灌胃还原型谷胱甘肽生理盐水溶液(0.12 m L/kg),同时除正常对照组外,其余4组给予灌胃40%乙醇(0.12 m L/kg),每日1次,连续16周。16周后,末次给乙醇12 h后处死小鼠采集血液样品和肝组织样品,测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和碱性磷酸酶(AKP)的活力,肝组织超氧化物歧化酶(SOD)活力、丙二醛含量(MDA),单胺氧化酶(MAO)的活力,并进行病理组织学检查。[结果]鳖甲寡肽I-C-F-6使灌胃乙醇后小鼠体内ALT、AST、MDA、MAO、AKP的含量显著降低(P<0.05),肝组织匀浆中SOD的活力明显升高(P<0.05),肝脏病理损伤减轻。[结论]鳖甲寡肽I-C-F-6对小鼠慢性酒精性肝损伤具有一定的预防保护作用,其机制可能与抑制脂质过氧化,清除自由基,升高SOD的活力有关。
文摘Enteric disorders in pigs are related to the fimbriae F4 (K88), F5 (K99), F6 (987P), F41 and F18 of enterotoxigenic Escherichia coli (ETEC). Immunization of sows with adhesins is important to stimulate the production of antibodies and the consequent transfer of these to the piglets via colostrum to prevent diarrhea during the neonate period and after weaning. The objective of this study was to evaluate the immune response of the sows immunized with recombinant ETEC proteins (F4, F5, F6, F18 and F41). The immune response of the sows immunized with the recombinant proteins was compared with a commercial vaccine containing ETEC bacterins. The study was performed on a commercial farm and included nine pregnant sows divided into three groups: G1 was vaccinated with recombinant proteins (n = 3);G2 was vaccinated with the commercial vaccine (n = 3);and G3 was vaccinated with sterile buffered saline (PBS) (n = 3). All the sows were fed a balanced diet without antibiotics and water ad libitum. The recombinant fimbriae stimulated the specific humoral immune response of the immunized sows. There was a statistically significant increase in the levels of antibodies to the fimbriae F4 (K88), F5 (K99), F6 (987P) and F18 in the sows vaccinated with the recombinant proteins compared with the control group. The colostrum IgG titers for all fimbriae in all the immunized sows were significantly increased compared to the control group. Additionally, all the piglets exhibited significantly increased antibody levels relative to all fimbriae when compared with those in the unimmunized control group, demonstrating successful antibody transfer via colostrum of the sows to the piglets.
