Chinese herbal medicines（CHMs）are playing important roles in the treatment of diseases and human health care throughout the world.However,the extrinsic harmful residues have been threatening the therapies by destroy...Chinese herbal medicines（CHMs）are playing important roles in the treatment of diseases and human health care throughout the world.However,the extrinsic harmful residues have been threatening the therapies by destroying CHMs qualtiy which hinders the international trades of CHMs.This review summarized the extrinsic harmful residues contaminating CHMs in types,detection methods,national and international regulations,and made safety evaluations according to their contaminated levels,aiming to establish understanding among nations or organizations and provide references for the formulation or amendment of relative regulations.展开更多
In order to implement 3D scanning of those complicated parts such as blades in the aviation field,a non-contact optical measuring system is established in the paper,which integrates a laser displacement sensor,a probe...In order to implement 3D scanning of those complicated parts such as blades in the aviation field,a non-contact optical measuring system is established in the paper,which integrates a laser displacement sensor,a probe head,the frame of a coordinate measuring machine（CMM）,etc.As the output of the laser sensor directly obtained possesses the 1D length of the laser beam,it needs to determine the unit direction vector of the laser beam denoted as（l,m,n）by calibration so as to convert the 1D values into 3D coordinates of target points.Therefore,an extrinsic calibration method based on a standard sphere is proposed to accomplish this task in the paper.During the calibration procedure,the laser sensor moves along with the motion of the CMM and gathers the required data on the spherical surface.Then,both the output of the laser sensor and the grating readings of the CMM are substituted into the constraint equation of the spherical surface,in which an over-determined nonlinear equation group containing unknown parameters is established.For the purpose of solving the equation group,a method based on non-linear least squares optimization is put forward.Finally,the system after calibration is utilized to measure the diameter of a metallic sphere 10 times from different orientations to verify the calibration accuracy.In the experiment,the errors between the measured results and the true values are all smaller than 0.03 mm,which manifests the validity and practicality of the extrinsic calibration method presented in the paper.展开更多
When implementing helicopter-satellite communications, periodical interruption of the received signal is a challenging problem because the communication antenna is intermittently blocked by the rotating blades of the ...When implementing helicopter-satellite communications, periodical interruption of the received signal is a challenging problem because the communication antenna is intermittently blocked by the rotating blades of the helicopter. The helicopter-satellite channel model and the Forward Error Control（FEC） coding countermeasure are presented in this paper. On the basis of this model, Check-Hybrid（CH） Low-Density Parity-Check（LDPC）codes are designed to mitigate the periodical blockage over the helicopter-satellite channels. The CH-LDPC code is derived by replacing part of single parity-check code constraints in a Quasi-Cyclic LDPC（QC-LDPC） code by using more powerful linear block code constraints. In particular, a method of optimizing the CH-LDPC code ensemble by searching the best matching component code among a variety of linear block codes using extrinsic information transfer charts is proposed. Simulation results show that, the CH-LDPC coding scheme designed for the helicopter-satellite channels in this paper achieves more than 25% bandwidth efficiency improvement, compared with the FEC scheme that uses QC-LDPC codes.展开更多
In this paper, we focus on the design of irregular QC-LDPC code based multi-level coded modulation(MLCM) scheme by jointly optimizing the component code rate and the degree distribution of the irregular QC-LDPC compon...In this paper, we focus on the design of irregular QC-LDPC code based multi-level coded modulation(MLCM) scheme by jointly optimizing the component code rate and the degree distribution of the irregular QC-LDPC component code. Firstly, the sub-channel capacities of MLCM systems is analyzed and discussed, based on which the optimal component code rate can be obtained. Secondly, an extrinsic information transfer chart based two-stage searching algorithm is proposed to find the good irregular QC-LDPC code ensembles with optimal component code rates for their corresponding sub-channels. Finally, by constructing the irregular QC-LDPC component codes from the designed ensembles with the aim of possibly enlarging the girth and reducing the number of the shortest cycles, the designed irregular QC-LDPC code based 16QAM and 64QAM MLCM systems can achieve 0.4 dB and 1.2 dB net coding gain, respectively, compared with the recently proposed regular QC-LDPC code based 16QAM and 64QAM MLCM systems.展开更多
In this paper,we propose a new algorithm to establish the data association between a camera and a 2-D Light Detection And Ranging sensor （LIDAR）.In contrast to the previous works,where data association is establishe...In this paper,we propose a new algorithm to establish the data association between a camera and a 2-D Light Detection And Ranging sensor （LIDAR）.In contrast to the previous works,where data association is established by calibrating the intrinsic parameters of the camera and the extrinsic parameters of the camera and the LIDAR,we formulate the map between laser points and pixels as a 2-D homography.The line-point correspondence is employed to construct geometric constraint on the homography matrix.This enables checkerboard to be not essential and any object with straight boundary can be an effective target.The calculation of the 2-D homography matrix consists of a linear least-squares solution of a homogeneous system followed by a nonlinear minimization of the geometric error in the image plane.Since the measurement quality impacts on the accuracy of the result,we investigate the equivalent constraint and show that placing the calibration target nearby the 2-D LIDAR will provide sufficient constraints to calculate the 2-D homography matrix.Simulation and experimental results validate that the proposed algorithm is robust and accurate.Compared with the previous works,which require two calibration processes and special calibration targets such as checkerboard,our method is more flexible and easier to perform.展开更多
Background: Extrinsic aging results from environmental stressors such as UVR or pollutants. While the effects of single pollutants are better understood, those of their combination remain poorly scrutinized. Objective...Background: Extrinsic aging results from environmental stressors such as UVR or pollutants. While the effects of single pollutants are better understood, those of their combination remain poorly scrutinized. Objective: Building on a study showing downregulation of several processes upon co-exposure to B[a]P and UVA, we investigated changes induced by epigenetic marks. Materials and Methods: Human primary fibroblasts and HaCaT cells were exposed to B[a]P and UVA. After 24 hours, exposed and unexposed cells were compared to assess DNA methylation. Focusing on HaCaT, multiplex assays enabled quantifying histone H3 modifications and evaluating four splicing factors (SRSF1, SRSF3, SFPQ, and SF3B1) by immunohistochemical labeling. The expression of keratinocyte-/fibroblast-relevant genes was assessed by RT-qPCR. Finally, the impact of an Arundo donax L. extract added 24 hours before B[a]P-UVA co-exposure was analyzed. Results: Exposure to B[a]P-UVA raised DNA methylation (HaCaT: ×3.6, fibroblasts: ×1.9), an increase prevented by the extract. In HaCaT cells, B[a]P-UVA increases the frequency of S10P (+38%). When exposure was preceded by extract treatment, the frequency of several methylations was impacted. B[a]P-UVA only induced the expression of SRSF1 and SFPQ in HaCaT (+46% and +34%). Treatment with the extract abolished this effect. Co-exposure increases the expression of inflammation-related genes (IL-1α, IL-1β) in HaCaT cells and decreases those of AQP3, KRT15, and SOD2. The extract has little effect on these changes. In primary fibroblasts, exposure to B[a]P-UVA lowered the expression of LOXL2, LUM, and TGFBR2 (−38%, −59%, and −51%, respectively), and the extract did not affect these modifications. Conclusion: Within 24 hours, a single B[a]P-UVA co-exposure changes epigenetic marks of skin cells but has only mild effects on gene expression. An Arundo donax L. extract can prevent part of the epigenetic marks’ changes and could stimulate the expression of some genes in primary fibroblasts.展开更多
Adult neurogenesis involves persistent proliferative neuroprogenitor populations that reside within distinct regions of the brain.This phenomenon was first described over 50 years ago and it is now firmly established ...Adult neurogenesis involves persistent proliferative neuroprogenitor populations that reside within distinct regions of the brain.This phenomenon was first described over 50 years ago and it is now firmly established that new neurons are continually generated in distinct regions of the adult brain.The potential of enhancing the neurogenic process lies in improved brain cognition and neuronal plasticity particularly in the context of neuronal injury and neurodegenerative disorders.In addition,adult neurogenesis might also play a role in mood and affective disorders.The factors that regulate adult neurogenesis have been broadly studied.However,the underlying molecular mechanisms of regulating neurogenesis are still not fully defined.In this review,we will provide critical analysis of our current understanding of the factors and molecular mechanisms that determine neurogenesis.We will further discuss pre-clinical and clinical studies that have investigated the potential of modulating neurogenesis as therapeutic intervention in neurodegeneration.展开更多
Physical literacy is a concept that is expected to encompass the mind and body in an integrated way to explain, promote, and help sustain human beings＇ fundamental function： movement. According to Whitehead（2010）,...Physical literacy is a concept that is expected to encompass the mind and body in an integrated way to explain, promote, and help sustain human beings＇ fundamental function： movement. According to Whitehead（2010）, physical literacy is defined by motivation, especially by competence-based and interest-based motivation. This point of view is consistent with vast amount of research evidence on children and adolescents＇ physical activity behavior. In the article I attempt to interpret and operationalize physical literacy from a perspective that children＇s motivation in physical education is both an innate mental disposition and an acquired/learned attribute. Particularly I rely on the conceptual learning theory and motivation regulation mechanisms of the self-determination theory to argue that in physical education, children should experience tasks that inspire them to embody competence and interest along with self-regulation strategies necessary for developing and sustaining the motivation to move.展开更多
BACKGROUND:Out-of-hospital cardiac arrest(OHCA) is a public health concern, and many studies have been conducted on return of spontaneous circulation(ROSC) and its prognostic factors.Rotational thromboelastometry(ROTE...BACKGROUND:Out-of-hospital cardiac arrest(OHCA) is a public health concern, and many studies have been conducted on return of spontaneous circulation(ROSC) and its prognostic factors.Rotational thromboelastometry(ROTEM?), a point-of-care testing(POCT) method, has been useful for predicting ROSC in patients with OHCA, but very few studies have focused on patients with non-shockable rhythm. We examined whether the parameters of POCT could predict ROSC in patients with OHCA and accompanying non-shockable rhythm.METHODS:This is a single-center, retrospective observational study. Complete blood count,blood gas, and ROTEM POCT measurements were used. This study included patients with nontraumatic OHCA aged 18 years or older who were transported to the emergency department and evaluated using POCT between January 2013 and December 2021. The patients were divided into the ROSC and non-ROSC groups. Prehospital information and POCT parameters were compared using receiver operating characteristic(ROC) curve analysis, and further logistic regression analysis was performed.RESULTS:Sixty-seven and 135 patients were in the ROSC and non-ROSC groups,respectively. The ROC curves showed a high area under the curve(AUC) for K^(+) of 0.77(95%confidence interval [CI]:0.71–0.83) and EXTEM amplitude 5 min after clotting time(A5) of 0.70(95%CI:0.62–0.77). The odds ratios for ROSC were as follows:female sex 3.67(95%CI:1.67–8.04);K^(+)0.64(95%CI:0.48–0.84);and EXTEM A5 1.03(95%CI:1.01–1.06).CONCLUSION:In OHCA patients with non-shockable rhythm, K^(+) level and the ROTEM parameter EXTEM A5 may be useful in predicting ROSC.展开更多
PHOTOSYSTEM Ⅱ（PS Ⅱ） particles capable of O<sub>2</sub> evolution contain D1, D2 transmembrane pro-teins, and three extrinsic polypeptides which are exposed to the outer aqueous phase with ap-parent mol...PHOTOSYSTEM Ⅱ（PS Ⅱ） particles capable of O<sub>2</sub> evolution contain D1, D2 transmembrane pro-teins, and three extrinsic polypeptides which are exposed to the outer aqueous phase with ap-parent molecular masses of 33, 23, 18 kD. The three proteins can be partially or totally re-moved by treating the PS Ⅱ membranes with high concentrations of salt or other methods,展开更多
The influence of digalactosyldiacylglycerol (DGDG), one of the photosynthetic membrane lipids, on heat inactivation of the process of oxygen evolution has been studied in vitro in photosystem Ⅱ(PS Ⅱ) core complex. I...The influence of digalactosyldiacylglycerol (DGDG), one of the photosynthetic membrane lipids, on heat inactivation of the process of oxygen evolution has been studied in vitro in photosystem Ⅱ(PS Ⅱ) core complex. It was found that the temperature of semi-inactivation of oxygen evolution in the complex increased from 40.0 to about 43.0℃ in the presence of DGDG with 5-min heat treatment in the dark. Furthermore, when PS Ⅱ core complex was incubated for 5 min at 45.0℃, the oxygen evolution in the complex was completely lost, whilst the DGDG-complexed PS Ⅱ core complex still retained a 16% of activity (100% for 25.0℃). In addition, a 1-h incubation at 38.0℃ inactivated absolutely the oxygen evolution for the PS Ⅱ core complex. By contrast, there remained about 20% of activity (zero time for 100%) for the complex in the presence of DGDG under the same condition. These results indicate a new role of DGDG in the protection of PS Ⅱ core complex against the deleterious effects of temperature. It was展开更多
In this paper,we present a new class of spatially coupled codes obtained by using both non-recursive and recursive block-oriented superposition.The resulting codes are termed as bidirectional block Markov superpositio...In this paper,we present a new class of spatially coupled codes obtained by using both non-recursive and recursive block-oriented superposition.The resulting codes are termed as bidirectional block Markov superposition transmission(BiBMST)codes.Firstly,we perform an iterative decoding threshold analysis according to protograph-based extrinsic information transfer(PEXIT)charts for the BiBMST codes over the binary erasure channels(BECs).Secondly,we derive the generator and parity-check matrices of the BiBMST codes.Thirdly,extensive numerical results are presented to show the advantages of the proposed BiBMST codes.Particularly,our numerical results show that,under the constraint of an equal decoding latency,the BiBMST codes perform better than the recursive BMST(rBMST)codes.However,the simulation results show that,in finite-length regime,negligible performance gain is obtained by increasing the encoding memory.We solve this limitation by introducing partial superposition,and the resulting codes are termed as partially-connected BiBMST(PC-BiBMST)code.Analytical results have confirmed the advantages of the PC-BiBMST codes over the original BiBMST codes.We also present extensive simulation results to show the performance advantages of the PC-BiBMST codes over the spatially coupled low-density parity-check(SC-LDPC)codes,spatially coupled generalized LDPC(SC-GLDPC)codes,and the original BiBMST codes in the finite-length regime.展开更多
AIM: To explore the feasibility of enhancing apoptosis-inducing effects of chemotherapeutic drugs on human gastric cancer cells by stable transfection of extrinsic Smac gene. METHODS: After Smac gene was transferred i...AIM: To explore the feasibility of enhancing apoptosis-inducing effects of chemotherapeutic drugs on human gastric cancer cells by stable transfection of extrinsic Smac gene. METHODS: After Smac gene was transferred into gastric cancer cell line MKN-45, subclone cells were obtained by persistent G_(418) selection. Cellular Smac gene expression was determined by RT-PCR and Western blotting. After treatment with mitomycin (MMC) as an apoptotic inducer, in vitro cell growth activities were investigated by trypan blue-staining method and MTT colorimetry. Cell apoptosis and its rates were determined by electronic microscopy, annexin V-FTTC and propidium iodide staining flow cytometry. Cellular caspase-3 protein expression and its activities were assayed by Western blotting and colorimetry. RESULTS: When compared with MKN-45 cells, the selected subclone cell line MKN-45/Smac had significantly higher Smac mRNA (3.12±0.21 vs 0.82±0.14, t=7.52, P＜0.01) and protein levels (4.02±0.24 vs0.98±0.11, t=8.32, P＜0.01). After treatment with 10 μg/mL MMC for 6-24 h, growth inhibition rate of MKN-45/Smac (15.8±1.2-54.8±2.9%) was significantly higher than that of MKN-45 (5.8±0.4-24.0±1.5%, t=6.42, P＜0.01). Partial MKN-45/Smac cancer cells presented characteristic morphological changes of apoptosis under the electronic microscope with an apoptosis rate of 36.4±2.1%, which was significantly higher than that of MKN-45 (15.2±0.8%, t=9.25, P＜0.01). Compared with MKN-45, caspase-3 expression levels in MKN-45/Smac were improved significantly (3.39±0.42 vs0.96±0.14, t=8.63, P＜0.01), while its activities were 3.25 times as many as those of MKN-45 (0.364±0.010 vs0.112±0.007, t=6.34, P＜0.01). CONCLUSION: Stable transfection of extrinsic Smac gene and its over-expression in gastric cancer cell line can significantly enhance cellular caspase-3 expression and activities, ameliorate apoptosis-inducing effects of mitomycin C on cancer cells, which is a novel strategy to improve chemotherapeutic effects on gastric cancer.展开更多
In this paper,we aim to design a practical low complexity low-density parity-check(LDPC)coded scheme to build a secure open channel and protect information from eavesdropping.To this end,we first propose a punctured L...In this paper,we aim to design a practical low complexity low-density parity-check(LDPC)coded scheme to build a secure open channel and protect information from eavesdropping.To this end,we first propose a punctured LDPC coded scheme,where the information bits in a codeword are punctured and only the parity check bits are transmitted to the receiver.We further propose a notion of check node type distribution and derive multi-edge type extrinsic information transfer functions to estimate the security performance,instead of the well-known weak metric bit error rate.We optimize the check node type distribution in terms of the signal-to-noise ratio(SNR)gap and modify the progressive edge growth algorithm to design finite-length codes.Numerical results show that our proposed scheme can achieve a lower computational complexity and a smaller security gap,compared to the existing scrambling and puncturing schemes.展开更多
Recent developments in spectral white-light interferometry(WLI)are reviewed.Firstly,the techniques for obtaining optical spectrum are introduced.Secondly,some novel measurement techniques are reviewed,including the im...Recent developments in spectral white-light interferometry(WLI)are reviewed.Firstly,the techniques for obtaining optical spectrum are introduced.Secondly,some novel measurement techniques are reviewed,including the improved peak-to-peak WLI,improved wavelength-tracking WLI,Fourier transform WLI,and 3×3 coupler based WLI.Furthermore,a hybrid measurement for the intensity-type sensors,interferometric sensors,and fiber Bragg grating sensors is achieved.It is shown that these developments have assisted in the progress of WLI.展开更多
基金supported by CAMS Innovation Fund for Medical Sciences (No. 2016-I2M-1-012, 2017-I2M-1-013)National Natural Science Foundation of China (No. 81573595, 81703699)National Project for Standardization of Chinese Materia Medica (No. ZYBZH-Y-JIN-34)
文摘Chinese herbal medicines（CHMs）are playing important roles in the treatment of diseases and human health care throughout the world.However,the extrinsic harmful residues have been threatening the therapies by destroying CHMs qualtiy which hinders the international trades of CHMs.This review summarized the extrinsic harmful residues contaminating CHMs in types,detection methods,national and international regulations,and made safety evaluations according to their contaminated levels,aiming to establish understanding among nations or organizations and provide references for the formulation or amendment of relative regulations.
基金supported by the National Science and Technology Major Project for ‘‘High-grade Numerical Control Machine Tools and Basic Manufacturing Equipment” of China (No. 2013ZX04001071)
文摘In order to implement 3D scanning of those complicated parts such as blades in the aviation field,a non-contact optical measuring system is established in the paper,which integrates a laser displacement sensor,a probe head,the frame of a coordinate measuring machine（CMM）,etc.As the output of the laser sensor directly obtained possesses the 1D length of the laser beam,it needs to determine the unit direction vector of the laser beam denoted as（l,m,n）by calibration so as to convert the 1D values into 3D coordinates of target points.Therefore,an extrinsic calibration method based on a standard sphere is proposed to accomplish this task in the paper.During the calibration procedure,the laser sensor moves along with the motion of the CMM and gathers the required data on the spherical surface.Then,both the output of the laser sensor and the grating readings of the CMM are substituted into the constraint equation of the spherical surface,in which an over-determined nonlinear equation group containing unknown parameters is established.For the purpose of solving the equation group,a method based on non-linear least squares optimization is put forward.Finally,the system after calibration is utilized to measure the diameter of a metallic sphere 10 times from different orientations to verify the calibration accuracy.In the experiment,the errors between the measured results and the true values are all smaller than 0.03 mm,which manifests the validity and practicality of the extrinsic calibration method presented in the paper.
基金supported by the National Natural Science Foundation of China(No.91538203)the new strategic industries development projects of Shenzhen City(No.JCYJ20150403155812833)
文摘When implementing helicopter-satellite communications, periodical interruption of the received signal is a challenging problem because the communication antenna is intermittently blocked by the rotating blades of the helicopter. The helicopter-satellite channel model and the Forward Error Control（FEC） coding countermeasure are presented in this paper. On the basis of this model, Check-Hybrid（CH） Low-Density Parity-Check（LDPC）codes are designed to mitigate the periodical blockage over the helicopter-satellite channels. The CH-LDPC code is derived by replacing part of single parity-check code constraints in a Quasi-Cyclic LDPC（QC-LDPC） code by using more powerful linear block code constraints. In particular, a method of optimizing the CH-LDPC code ensemble by searching the best matching component code among a variety of linear block codes using extrinsic information transfer charts is proposed. Simulation results show that, the CH-LDPC coding scheme designed for the helicopter-satellite channels in this paper achieves more than 25% bandwidth efficiency improvement, compared with the FEC scheme that uses QC-LDPC codes.
基金supported by National Natural Science Foundation of China(No.61571061)
文摘In this paper, we focus on the design of irregular QC-LDPC code based multi-level coded modulation(MLCM) scheme by jointly optimizing the component code rate and the degree distribution of the irregular QC-LDPC component code. Firstly, the sub-channel capacities of MLCM systems is analyzed and discussed, based on which the optimal component code rate can be obtained. Secondly, an extrinsic information transfer chart based two-stage searching algorithm is proposed to find the good irregular QC-LDPC code ensembles with optimal component code rates for their corresponding sub-channels. Finally, by constructing the irregular QC-LDPC component codes from the designed ensembles with the aim of possibly enlarging the girth and reducing the number of the shortest cycles, the designed irregular QC-LDPC code based 16QAM and 64QAM MLCM systems can achieve 0.4 dB and 1.2 dB net coding gain, respectively, compared with the recently proposed regular QC-LDPC code based 16QAM and 64QAM MLCM systems.
基金supported in part by the National Natural Science Foundation of China (Nos. 90820305 and 60775040)the National High-Tech Research and Development (863) Program of China (No. 2012AA041402)
文摘In this paper,we propose a new algorithm to establish the data association between a camera and a 2-D Light Detection And Ranging sensor （LIDAR）.In contrast to the previous works,where data association is established by calibrating the intrinsic parameters of the camera and the extrinsic parameters of the camera and the LIDAR,we formulate the map between laser points and pixels as a 2-D homography.The line-point correspondence is employed to construct geometric constraint on the homography matrix.This enables checkerboard to be not essential and any object with straight boundary can be an effective target.The calculation of the 2-D homography matrix consists of a linear least-squares solution of a homogeneous system followed by a nonlinear minimization of the geometric error in the image plane.Since the measurement quality impacts on the accuracy of the result,we investigate the equivalent constraint and show that placing the calibration target nearby the 2-D LIDAR will provide sufficient constraints to calculate the 2-D homography matrix.Simulation and experimental results validate that the proposed algorithm is robust and accurate.Compared with the previous works,which require two calibration processes and special calibration targets such as checkerboard,our method is more flexible and easier to perform.
文摘Background: Extrinsic aging results from environmental stressors such as UVR or pollutants. While the effects of single pollutants are better understood, those of their combination remain poorly scrutinized. Objective: Building on a study showing downregulation of several processes upon co-exposure to B[a]P and UVA, we investigated changes induced by epigenetic marks. Materials and Methods: Human primary fibroblasts and HaCaT cells were exposed to B[a]P and UVA. After 24 hours, exposed and unexposed cells were compared to assess DNA methylation. Focusing on HaCaT, multiplex assays enabled quantifying histone H3 modifications and evaluating four splicing factors (SRSF1, SRSF3, SFPQ, and SF3B1) by immunohistochemical labeling. The expression of keratinocyte-/fibroblast-relevant genes was assessed by RT-qPCR. Finally, the impact of an Arundo donax L. extract added 24 hours before B[a]P-UVA co-exposure was analyzed. Results: Exposure to B[a]P-UVA raised DNA methylation (HaCaT: ×3.6, fibroblasts: ×1.9), an increase prevented by the extract. In HaCaT cells, B[a]P-UVA increases the frequency of S10P (+38%). When exposure was preceded by extract treatment, the frequency of several methylations was impacted. B[a]P-UVA only induced the expression of SRSF1 and SFPQ in HaCaT (+46% and +34%). Treatment with the extract abolished this effect. Co-exposure increases the expression of inflammation-related genes (IL-1α, IL-1β) in HaCaT cells and decreases those of AQP3, KRT15, and SOD2. The extract has little effect on these changes. In primary fibroblasts, exposure to B[a]P-UVA lowered the expression of LOXL2, LUM, and TGFBR2 (−38%, −59%, and −51%, respectively), and the extract did not affect these modifications. Conclusion: Within 24 hours, a single B[a]P-UVA co-exposure changes epigenetic marks of skin cells but has only mild effects on gene expression. An Arundo donax L. extract can prevent part of the epigenetic marks’ changes and could stimulate the expression of some genes in primary fibroblasts.
基金DN is a recipient of an ARC Future Fellowship(FT120100193)a Cancer Council Queensland Grant-in-Aid(1101931).
文摘Adult neurogenesis involves persistent proliferative neuroprogenitor populations that reside within distinct regions of the brain.This phenomenon was first described over 50 years ago and it is now firmly established that new neurons are continually generated in distinct regions of the adult brain.The potential of enhancing the neurogenic process lies in improved brain cognition and neuronal plasticity particularly in the context of neuronal injury and neurodegenerative disorders.In addition,adult neurogenesis might also play a role in mood and affective disorders.The factors that regulate adult neurogenesis have been broadly studied.However,the underlying molecular mechanisms of regulating neurogenesis are still not fully defined.In this review,we will provide critical analysis of our current understanding of the factors and molecular mechanisms that determine neurogenesis.We will further discuss pre-clinical and clinical studies that have investigated the potential of modulating neurogenesis as therapeutic intervention in neurodegeneration.
文摘Physical literacy is a concept that is expected to encompass the mind and body in an integrated way to explain, promote, and help sustain human beings＇ fundamental function： movement. According to Whitehead（2010）, physical literacy is defined by motivation, especially by competence-based and interest-based motivation. This point of view is consistent with vast amount of research evidence on children and adolescents＇ physical activity behavior. In the article I attempt to interpret and operationalize physical literacy from a perspective that children＇s motivation in physical education is both an innate mental disposition and an acquired/learned attribute. Particularly I rely on the conceptual learning theory and motivation regulation mechanisms of the self-determination theory to argue that in physical education, children should experience tasks that inspire them to embody competence and interest along with self-regulation strategies necessary for developing and sustaining the motivation to move.
文摘BACKGROUND:Out-of-hospital cardiac arrest(OHCA) is a public health concern, and many studies have been conducted on return of spontaneous circulation(ROSC) and its prognostic factors.Rotational thromboelastometry(ROTEM?), a point-of-care testing(POCT) method, has been useful for predicting ROSC in patients with OHCA, but very few studies have focused on patients with non-shockable rhythm. We examined whether the parameters of POCT could predict ROSC in patients with OHCA and accompanying non-shockable rhythm.METHODS:This is a single-center, retrospective observational study. Complete blood count,blood gas, and ROTEM POCT measurements were used. This study included patients with nontraumatic OHCA aged 18 years or older who were transported to the emergency department and evaluated using POCT between January 2013 and December 2021. The patients were divided into the ROSC and non-ROSC groups. Prehospital information and POCT parameters were compared using receiver operating characteristic(ROC) curve analysis, and further logistic regression analysis was performed.RESULTS:Sixty-seven and 135 patients were in the ROSC and non-ROSC groups,respectively. The ROC curves showed a high area under the curve(AUC) for K^(+) of 0.77(95%confidence interval [CI]:0.71–0.83) and EXTEM amplitude 5 min after clotting time(A5) of 0.70(95%CI:0.62–0.77). The odds ratios for ROSC were as follows:female sex 3.67(95%CI:1.67–8.04);K^(+)0.64(95%CI:0.48–0.84);and EXTEM A5 1.03(95%CI:1.01–1.06).CONCLUSION:In OHCA patients with non-shockable rhythm, K^(+) level and the ROTEM parameter EXTEM A5 may be useful in predicting ROSC.
文摘PHOTOSYSTEM Ⅱ（PS Ⅱ） particles capable of O<sub>2</sub> evolution contain D1, D2 transmembrane pro-teins, and three extrinsic polypeptides which are exposed to the outer aqueous phase with ap-parent molecular masses of 33, 23, 18 kD. The three proteins can be partially or totally re-moved by treating the PS Ⅱ membranes with high concentrations of salt or other methods,
基金This work was supported by the National Natural Science Foundation of China (Grant No. 39890390)the State Basic Research Development Plan of China (Grant No. G1998010100)the Innovative Foundation of Laboratory of Photosynthesis Basic Research, In
文摘The influence of digalactosyldiacylglycerol (DGDG), one of the photosynthetic membrane lipids, on heat inactivation of the process of oxygen evolution has been studied in vitro in photosystem Ⅱ(PS Ⅱ) core complex. It was found that the temperature of semi-inactivation of oxygen evolution in the complex increased from 40.0 to about 43.0℃ in the presence of DGDG with 5-min heat treatment in the dark. Furthermore, when PS Ⅱ core complex was incubated for 5 min at 45.0℃, the oxygen evolution in the complex was completely lost, whilst the DGDG-complexed PS Ⅱ core complex still retained a 16% of activity (100% for 25.0℃). In addition, a 1-h incubation at 38.0℃ inactivated absolutely the oxygen evolution for the PS Ⅱ core complex. By contrast, there remained about 20% of activity (zero time for 100%) for the complex in the presence of DGDG under the same condition. These results indicate a new role of DGDG in the protection of PS Ⅱ core complex against the deleterious effects of temperature. It was
基金supported by the National Natural Science Foundation of China(Nos.62271233,12271215,and 62261003)the Basic Research Program of Guangzhou Municipal Science and Technology Bureau(No.202201020036)the Guangdong Provincial Natural Science Foundation(Nos.2022A1515010029 and 2021A1515011906).
文摘In this paper,we present a new class of spatially coupled codes obtained by using both non-recursive and recursive block-oriented superposition.The resulting codes are termed as bidirectional block Markov superposition transmission(BiBMST)codes.Firstly,we perform an iterative decoding threshold analysis according to protograph-based extrinsic information transfer(PEXIT)charts for the BiBMST codes over the binary erasure channels(BECs).Secondly,we derive the generator and parity-check matrices of the BiBMST codes.Thirdly,extensive numerical results are presented to show the advantages of the proposed BiBMST codes.Particularly,our numerical results show that,under the constraint of an equal decoding latency,the BiBMST codes perform better than the recursive BMST(rBMST)codes.However,the simulation results show that,in finite-length regime,negligible performance gain is obtained by increasing the encoding memory.We solve this limitation by introducing partial superposition,and the resulting codes are termed as partially-connected BiBMST(PC-BiBMST)code.Analytical results have confirmed the advantages of the PC-BiBMST codes over the original BiBMST codes.We also present extensive simulation results to show the performance advantages of the PC-BiBMST codes over the spatially coupled low-density parity-check(SC-LDPC)codes,spatially coupled generalized LDPC(SC-GLDPC)codes,and the original BiBMST codes in the finite-length regime.
文摘AIM: To explore the feasibility of enhancing apoptosis-inducing effects of chemotherapeutic drugs on human gastric cancer cells by stable transfection of extrinsic Smac gene. METHODS: After Smac gene was transferred into gastric cancer cell line MKN-45, subclone cells were obtained by persistent G_(418) selection. Cellular Smac gene expression was determined by RT-PCR and Western blotting. After treatment with mitomycin (MMC) as an apoptotic inducer, in vitro cell growth activities were investigated by trypan blue-staining method and MTT colorimetry. Cell apoptosis and its rates were determined by electronic microscopy, annexin V-FTTC and propidium iodide staining flow cytometry. Cellular caspase-3 protein expression and its activities were assayed by Western blotting and colorimetry. RESULTS: When compared with MKN-45 cells, the selected subclone cell line MKN-45/Smac had significantly higher Smac mRNA (3.12±0.21 vs 0.82±0.14, t=7.52, P＜0.01) and protein levels (4.02±0.24 vs0.98±0.11, t=8.32, P＜0.01). After treatment with 10 μg/mL MMC for 6-24 h, growth inhibition rate of MKN-45/Smac (15.8±1.2-54.8±2.9%) was significantly higher than that of MKN-45 (5.8±0.4-24.0±1.5%, t=6.42, P＜0.01). Partial MKN-45/Smac cancer cells presented characteristic morphological changes of apoptosis under the electronic microscope with an apoptosis rate of 36.4±2.1%, which was significantly higher than that of MKN-45 (15.2±0.8%, t=9.25, P＜0.01). Compared with MKN-45, caspase-3 expression levels in MKN-45/Smac were improved significantly (3.39±0.42 vs0.96±0.14, t=8.63, P＜0.01), while its activities were 3.25 times as many as those of MKN-45 (0.364±0.010 vs0.112±0.007, t=6.34, P＜0.01). CONCLUSION: Stable transfection of extrinsic Smac gene and its over-expression in gastric cancer cell line can significantly enhance cellular caspase-3 expression and activities, ameliorate apoptosis-inducing effects of mitomycin C on cancer cells, which is a novel strategy to improve chemotherapeutic effects on gastric cancer.
文摘In this paper,we aim to design a practical low complexity low-density parity-check(LDPC)coded scheme to build a secure open channel and protect information from eavesdropping.To this end,we first propose a punctured LDPC coded scheme,where the information bits in a codeword are punctured and only the parity check bits are transmitted to the receiver.We further propose a notion of check node type distribution and derive multi-edge type extrinsic information transfer functions to estimate the security performance,instead of the well-known weak metric bit error rate.We optimize the check node type distribution in terms of the signal-to-noise ratio(SNR)gap and modify the progressive edge growth algorithm to design finite-length codes.Numerical results show that our proposed scheme can achieve a lower computational complexity and a smaller security gap,compared to the existing scrambling and puncturing schemes.
基金This work was supported by the National Natural Scientific Foundation of China(51075037)the Program for New Century Excellent Talents(NCET)at the University of China and Chinese 863 Project(2008AA04Z406).
文摘Recent developments in spectral white-light interferometry(WLI)are reviewed.Firstly,the techniques for obtaining optical spectrum are introduced.Secondly,some novel measurement techniques are reviewed,including the improved peak-to-peak WLI,improved wavelength-tracking WLI,Fourier transform WLI,and 3×3 coupler based WLI.Furthermore,a hybrid measurement for the intensity-type sensors,interferometric sensors,and fiber Bragg grating sensors is achieved.It is shown that these developments have assisted in the progress of WLI.
