With supercritical CO2 fluid extraction（SCFE）, essential oil was extracted from three cultivars of Xianning osmanthus. The fresh osmanthus flower was processed with a petroleum ether digestion method to produce the ...With supercritical CO2 fluid extraction（SCFE）, essential oil was extracted from three cultivars of Xianning osmanthus. The fresh osmanthus flower was processed with a petroleum ether digestion method to produce the extractum. The yields of essential oil and extractum were 0.19 % and 0.13 % （m/m） respectively. The essential oil and fragrance composition and content extracted were analyzed with gas chromatography-mass spectrometer （GC-MS）. The result showed that essential oil contained 36.99%（area/total area） of ionone, ionol and 13.11% of linalool; ionone and ionol contained in extractum were as high as up to 33.33%, while linalool up to 21.92%. Whether essential oil or extractum contains only about 40% fat acid and other ester matters. None of environmental estrogen （phthalic ester） was found in fragrance ingredients. The result also showed that the quality of O. fragrans Albus group fragrance in Xianning is better than that produced in Hangzhou and Anhui districts.展开更多
基金Supported by the Manufacture-Learning-Research Cooperation Funded Projects of the Education Department in Hubei Province(C2010064)
文摘With supercritical CO2 fluid extraction（SCFE）, essential oil was extracted from three cultivars of Xianning osmanthus. The fresh osmanthus flower was processed with a petroleum ether digestion method to produce the extractum. The yields of essential oil and extractum were 0.19 % and 0.13 % （m/m） respectively. The essential oil and fragrance composition and content extracted were analyzed with gas chromatography-mass spectrometer （GC-MS）. The result showed that essential oil contained 36.99%（area/total area） of ionone, ionol and 13.11% of linalool; ionone and ionol contained in extractum were as high as up to 33.33%, while linalool up to 21.92%. Whether essential oil or extractum contains only about 40% fat acid and other ester matters. None of environmental estrogen （phthalic ester） was found in fragrance ingredients. The result also showed that the quality of O. fragrans Albus group fragrance in Xianning is better than that produced in Hangzhou and Anhui districts.
文摘目的:观察加参方浸膏对过氧化氢(H_2O_2)诱导的H9c2心肌细胞凋亡的干预作用,并探讨其作用机制。方法:采用MTT法,考察400、200、100、50、25μmol/L的H_2O_2与3.0、1.8、0.9、0.3、0.1 mg/m L加参方浸膏对H9c2心肌细胞活性的影响。将H9c2心肌细胞分为正常组、模型组、加参方组,除正常组细胞加入相应培养基外,模型组、加参方组细胞均采用50μmol/L H_2O_2处理以诱导细胞发生氧化应激损伤,加参方组在模型组基础上加入0.3 mg/m L加参方浸膏干预处理。采用DAPI染色法,在荧光显微镜下观察细胞形态学变化;采用流式细胞仪检测细胞凋亡率;采用荧光定量逆转录-聚合酶链式反应法测定细胞中胱天蛋白酶3(Caspase-3)、Bax、Bcl-2 m RNA表达水平,Western Blot法测定Caspase-3、Bax、Bcl-2蛋白表达水平。结果:50μmol/L为H_2O_2最适造模浓度,0.3 mg/m L为加参方浸膏最适药物干预浓度。与模型组比较,加参方组细胞的形态改善,细胞凋亡率显著降低(P<0.05);Caspase-3、Bax m RNA及蛋白表达水平显著降低,Bcl-2 m RNA及蛋白表达水平显著升高(P<0.05)。结论:加参方浸膏对H_2O_2诱导的H9c2心肌细胞凋亡有显著的抑制作用,其机制可能与下调细胞中Caspase-3、Bax表达,上调Bcl-2表达有关。
