African swine fever(ASF) is a lethal hemorrhagic disease that affects wild and domestic swine. The etiological agent of ASF is African swine fever virus(ASFV). Since the first case was described in Kenya in 1921, the ...African swine fever(ASF) is a lethal hemorrhagic disease that affects wild and domestic swine. The etiological agent of ASF is African swine fever virus(ASFV). Since the first case was described in Kenya in 1921, the disease has spread to many other countries. No commercial vaccines are available to prevent ASF. In this study, we generated a recombinant Newcastle disease virus(r NDV) expressing ASFV protein 72(p72) by reverse genetics and evaluated its humoral and cellular immunogenicity in a mouse model. The recombinant virus, r NDV/p72, replicated well in embryonated chicken eggs and was safe to use in chicks and mice. The p72 gene in r NDV/p72 was stably maintained through ten passages. Mice immunized with r NDV/p72 developed high titers of ASFV p72 specific Ig G antibody, and had higher levels of Ig G1 than IgG2 a. Immunization also elicited T-cell proliferation and secretion of IFN-γ and IL-4. Taken together, these results indicate that r NDV expressing ASFV p72 might be a potential vaccine candidate for preventing ASF.展开更多
MYB transcription factors play vital roles in plant growth and metabolism.The phytohormone methyl jasmonate(MeJA)promotes phenolic acid accumulation in the medicinal herb Salvia miltiorrhiza,but the regulatory mechani...MYB transcription factors play vital roles in plant growth and metabolism.The phytohormone methyl jasmonate(MeJA)promotes phenolic acid accumulation in the medicinal herb Salvia miltiorrhiza,but the regulatory mechanism is poorly understood.Here,we identified the MeJA-responsive R2R3-MYB transcription factor gene 5mMYB2 from a transcriptome library produced from MeJAtreated S.mi'/horr/ifza hairy roots.Sm/VIYB2 expression was tightly correlated with the expression of key salvianolic acid biosynthetic genes including CYP^8Ai^.5mMYB2 was highly expressed in the periderm of S.miltiorrhiza and SmMYB2 localized to the nucleus.Overexpressing Sml\AYB2 in S.miltiorrhiza hairy roots significantly increased the levels of salvianolic acids(including rosmarinic acid and salvianolic acid B)by upregulating salvianolic acid biosynthetic genes such as CYP98A14.SmMYB2 binds to the MYB-binding motifs in the promoter of CYP98A14,as confirmed by a dual-luciferase assay and electrophoretic mobility shift assays.Anthocyanin contents were significantly higher in Sm/ViyB2-overexpressing hairy root lines than the control,primarily due to the increased expression of CHI,DFR,and ANS.These findings reveal the novel regulatory role of SmMYB2 in MeJA-mediated phenolic acid biosynthesis,providing a useful target gene for metabolic engineering and shedding light on the salvianolic acid regulatory network.展开更多
DNA-binding one zinc-finger(Dof)proteins constitute a family of transcription factors with a highly conserved Dof domain that contains a C2C2 zinc-finger motif.Although several studies have demonstrated that Dof prote...DNA-binding one zinc-finger(Dof)proteins constitute a family of transcription factors with a highly conserved Dof domain that contains a C2C2 zinc-finger motif.Although several studies have demonstrated that Dof proteins are involved in multiple plant processes,including development and stress resistance,the functions of these proteins in drought stress resistance are largely unknown.Here,we report the identification of the MdDof54 gene from apple and document its positive roles in apple drought resistance.After long-term drought stress,compared with nontransgenic plants,MdDof54 RNAi plants had significantly shorter heights and weaker root systems;the transgenic plants also had lower shoot and root hydraulic conductivity,as well as lower photosynthesis rates.By contrast,compared with nontransgenic plants,MdDof54-overexpressing plants had higher photosynthesis rates and shoot hydraulic conductivity under long-term drought stress.Moreover,compared with nontransgenic plants,MdDof54-overexpressing plants had higher survival percentages under short-term drought stress,whereas MdDof54 RNAi plants had lower survival percentages.MdDof54 RNAi plants showed significant downregulation of 99 genes and significant upregulation of 992 genes in response to drought,and 366 of these genes were responsive to drought.We used DAPseq and ChIP-seq analyses to demonstrate that MdDof54 recognizes cis-elements that contain an AAAG motif.Taken together,our results provide new information on the functions of MdDof54 in plant drought stress resistance as well as resources for apple breeding aimed at the improvement of drought resistance.展开更多
Dear Editor,Type1diabetes(T1D)isa lifelong(chronic)disease and a major health problem throughout the world.This disease can be treatedby either insulin injection or islet transplantation.Islet transplantation is consi...Dear Editor,Type1diabetes(T1D)isa lifelong(chronic)disease and a major health problem throughout the world.This disease can be treatedby either insulin injection or islet transplantation.Islet transplantation is considered as a better treatment for T1D patients,because islets can produce and release insulin at the appropriate time,resulting in tight blood glucose control.展开更多
The major structural protein gene was cloned from Bombyx mori densovirus,Zhenjiang strain （BmDNV-ZJ）,sequenced and expressed in Escherichia coli.The gene consists of 1500 nucleotides and is predicted to code a prote...The major structural protein gene was cloned from Bombyx mori densovirus,Zhenjiang strain （BmDNV-ZJ）,sequenced and expressed in Escherichia coli.The gene consists of 1500 nucleotides and is predicted to code a protein of 499 amino acids.An identity of 98.1% in nucleotide sequence and 98.6% of the predicted amino acid sequence are shared with its homologue,Bombyx mori densovirus （Yamanashi isolate） VD1-ORF2,respectively.The gene was inserted into expression plasmid vector pET-28a and expressed in Escherichia coli BL21（DE3） strain under the inducible promoter Lac.The expressed 53kD product could be detected by Western blotting with viral antiserum.It suggests that the gene we cloned is a structural protein gene.展开更多
基金financially supported by the National Supporting Program(2013BAD12B05)Natural Science Foundation of China(NSFC)(no.31101839 and no.31302117)+1 种基金the key program of Gansu Province(no.1013JHTA008)Jiangsu Co-innovation Center program for Prevention and Control of Important Animal Infectious Diseases and Zoonoses
文摘African swine fever(ASF) is a lethal hemorrhagic disease that affects wild and domestic swine. The etiological agent of ASF is African swine fever virus(ASFV). Since the first case was described in Kenya in 1921, the disease has spread to many other countries. No commercial vaccines are available to prevent ASF. In this study, we generated a recombinant Newcastle disease virus(r NDV) expressing ASFV protein 72(p72) by reverse genetics and evaluated its humoral and cellular immunogenicity in a mouse model. The recombinant virus, r NDV/p72, replicated well in embryonated chicken eggs and was safe to use in chicks and mice. The p72 gene in r NDV/p72 was stably maintained through ten passages. Mice immunized with r NDV/p72 developed high titers of ASFV p72 specific Ig G antibody, and had higher levels of Ig G1 than IgG2 a. Immunization also elicited T-cell proliferation and secretion of IFN-γ and IL-4. Taken together, these results indicate that r NDV expressing ASFV p72 might be a potential vaccine candidate for preventing ASF.
基金This work was supported by National Natural Science Fund of China(81522049,31571735,31270007,30900110)the“Dawn”Program of Shanghai Education Commission(16SG38),Shanghai Science and Technology Committee Project(17JC1404300,15430502700),Zhejiang Provincial Wanren Program for Leading Talents of Science and Technology Innovation(2018R52050)+2 种基金Zhejiang Provincial Program for the Cultivation of High-level Innovative Health talents(2018-62-3)New Century Talent Project(NECT-13-0902)Opening Project of Zhejiang Provincial Preponderant and Characteristic Subject of Key University(Traditional Chinese Pharmacology),Zhejiang Chinese Medical University(ZYAOX2018019).
文摘MYB transcription factors play vital roles in plant growth and metabolism.The phytohormone methyl jasmonate(MeJA)promotes phenolic acid accumulation in the medicinal herb Salvia miltiorrhiza,but the regulatory mechanism is poorly understood.Here,we identified the MeJA-responsive R2R3-MYB transcription factor gene 5mMYB2 from a transcriptome library produced from MeJAtreated S.mi'/horr/ifza hairy roots.Sm/VIYB2 expression was tightly correlated with the expression of key salvianolic acid biosynthetic genes including CYP^8Ai^.5mMYB2 was highly expressed in the periderm of S.miltiorrhiza and SmMYB2 localized to the nucleus.Overexpressing Sml\AYB2 in S.miltiorrhiza hairy roots significantly increased the levels of salvianolic acids(including rosmarinic acid and salvianolic acid B)by upregulating salvianolic acid biosynthetic genes such as CYP98A14.SmMYB2 binds to the MYB-binding motifs in the promoter of CYP98A14,as confirmed by a dual-luciferase assay and electrophoretic mobility shift assays.Anthocyanin contents were significantly higher in Sm/ViyB2-overexpressing hairy root lines than the control,primarily due to the increased expression of CHI,DFR,and ANS.These findings reveal the novel regulatory role of SmMYB2 in MeJA-mediated phenolic acid biosynthesis,providing a useful target gene for metabolic engineering and shedding light on the salvianolic acid regulatory network.
基金supported by the National Natural Science Foundation of China(31572106 and 31622049).
文摘DNA-binding one zinc-finger(Dof)proteins constitute a family of transcription factors with a highly conserved Dof domain that contains a C2C2 zinc-finger motif.Although several studies have demonstrated that Dof proteins are involved in multiple plant processes,including development and stress resistance,the functions of these proteins in drought stress resistance are largely unknown.Here,we report the identification of the MdDof54 gene from apple and document its positive roles in apple drought resistance.After long-term drought stress,compared with nontransgenic plants,MdDof54 RNAi plants had significantly shorter heights and weaker root systems;the transgenic plants also had lower shoot and root hydraulic conductivity,as well as lower photosynthesis rates.By contrast,compared with nontransgenic plants,MdDof54-overexpressing plants had higher photosynthesis rates and shoot hydraulic conductivity under long-term drought stress.Moreover,compared with nontransgenic plants,MdDof54-overexpressing plants had higher survival percentages under short-term drought stress,whereas MdDof54 RNAi plants had lower survival percentages.MdDof54 RNAi plants showed significant downregulation of 99 genes and significant upregulation of 992 genes in response to drought,and 366 of these genes were responsive to drought.We used DAPseq and ChIP-seq analyses to demonstrate that MdDof54 recognizes cis-elements that contain an AAAG motif.Taken together,our results provide new information on the functions of MdDof54 in plant drought stress resistance as well as resources for apple breeding aimed at the improvement of drought resistance.
基金supported by grants from the National Basic Research Program of China (973 programs) (2011CB944203,2011CB944204)the National High-Tech R&D Program of China (863 Programs) (2014AA021602)+3 种基金the National Natural Science Foundation of China (31401271)the Key Deployment Project of the Chinese Academy of Sciences (KSZD-EW-Z-005-003-002)the Science and Technology Planning Project of Guangdong Province,China (2014B030301058,2015A030310119)Bureau of Science and Technology of GuangzhouMunicipality (201505011111498).
文摘Dear Editor,Type1diabetes(T1D)isa lifelong(chronic)disease and a major health problem throughout the world.This disease can be treatedby either insulin injection or islet transplantation.Islet transplantation is considered as a better treatment for T1D patients,because islets can produce and release insulin at the appropriate time,resulting in tight blood glucose control.
文摘The major structural protein gene was cloned from Bombyx mori densovirus,Zhenjiang strain （BmDNV-ZJ）,sequenced and expressed in Escherichia coli.The gene consists of 1500 nucleotides and is predicted to code a protein of 499 amino acids.An identity of 98.1% in nucleotide sequence and 98.6% of the predicted amino acid sequence are shared with its homologue,Bombyx mori densovirus （Yamanashi isolate） VD1-ORF2,respectively.The gene was inserted into expression plasmid vector pET-28a and expressed in Escherichia coli BL21（DE3） strain under the inducible promoter Lac.The expressed 53kD product could be detected by Western blotting with viral antiserum.It suggests that the gene we cloned is a structural protein gene.
