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薰衣草玻璃化组培苗逆转技术研究 被引量:8
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作者 戴丽娜 于志鹏 +4 位作者 吕国华 聂传胜 贾晓鹰 肖春燕 权俊萍 《新疆农业科学》 CAS CSCD 北大核心 2012年第11期2054-2061,F0003,共9页
【目的】比较研究不同培养基对薰衣草玻璃化组培苗形态及生理效应,为抑制组培苗生产中玻璃化现象及实施玻璃化苗逆转技术提供科学参考。【方法】以薰衣草玻璃化组培苗为材料,对培养基中MS大量元素、蔗糖、活性炭、聚乙烯毗咯烷酮(PV... 【目的】比较研究不同培养基对薰衣草玻璃化组培苗形态及生理效应,为抑制组培苗生产中玻璃化现象及实施玻璃化苗逆转技术提供科学参考。【方法】以薰衣草玻璃化组培苗为材料,对培养基中MS大量元素、蔗糖、活性炭、聚乙烯毗咯烷酮(PVP)等因素进行不同浓度L9(3^4)正交试验比较分析,在此基础上,比较研究不同激素培养基对玻璃化苗形态生理效应。【结果】从逆转率分析得知,PVP和Ms大量元素对逆转率影响分别达到极显著和显著,蔗糖和活性炭不显著;而综合评分分析可知,PVP对组培苗的逆向生长具有极显著效果,MS、蔗糖和活性炭不显著。不同浓度6-BA和KT对于玻璃化苗的逆转均有显著影响,彼此间表现极显著,KT逆转效果优于6-BA,分裂素浓度越高玻璃化越严重。【结论】配方:1/3MS+KT(0.1)+IBA(0.05)+PVP(2g/L)+活性炭(0.6%),不仅可显著抑制玻璃化苗的产生,而且对已经玻璃化的组培苗有显著逆转效果,逆转后的组培苗生长良好。 展开更多
关键词 薰衣草 组织培养 玻璃化 逆转
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NAA BA KT对麝香石竹不定芽形成的影响 被引量:3
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作者 曹新祥 《绍兴文理学院学报》 2001年第8期32-35,共4页
研究NAA,BA,KT对麝香石竹茎段外植体不定芽形成的影响.结果表明它们都能有效地诱导麝香石竹茎段外植体不定芽的形成,其中以BA诱导的芽的分化频率最高,生长素和细胞分裂素配合能进一步提高芽的分化频率.BA在高频率诱导不定芽形成的同时,... 研究NAA,BA,KT对麝香石竹茎段外植体不定芽形成的影响.结果表明它们都能有效地诱导麝香石竹茎段外植体不定芽的形成,其中以BA诱导的芽的分化频率最高,生长素和细胞分裂素配合能进一步提高芽的分化频率.BA在高频率诱导不定芽形成的同时,对分化芽的伸长生长、鲜干重的增长、干重百分率的提高和叶绿素水平的增加均有明显的促进作用,而KT,NAA则不然. 展开更多
关键词 麝香石竹 不定芽 分化芽 鲜重 干重 外植体
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A pilot study of intraocular lens explantation in 69 eyes in Chinese patients 被引量:1
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作者 Fang Chai Bo Ma +2 位作者 Xin-Guang Yang Juan Li Mei-Fang Chu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2017年第4期579-585,共7页
AIM:To study the effects of intraocular lens(IOL) explantation and demographic characteristics.METHODS:Retrospective non-comparative case series.Clinical data recorded from patient charts included the following:d... AIM:To study the effects of intraocular lens(IOL) explantation and demographic characteristics.METHODS:Retrospective non-comparative case series.Clinical data recorded from patient charts included the following:demographic,preoperative and postoperative characteristics;complications;surgical methods,and changes in visual acuity.RESULTS:A total of 69 eyes in 67 Chinese patients who received IOL explants were studied.The patients' mean age at the time of explantation was 46.1 years old [SD 22.5(6-85)],and 37 patients were female(55.2%).Regarding employment,47.8% were farmers,23.9% were retired,16.4% were students,4.5% were unemployed,3% were workers,and 4.5% were other(including staff members,teachers and officers).The main reasons for explantation were dislocation/decentration in 41 cases(59.4%) and retinal detachment in 10 cases(14.5%).The third most prevalent cause was incorrect lens power in 7 eyes(10.1%).The remaining reasons were endophthalmitis in 6 cases(8.7%),posterior capsular opacity in 3 eyes(4.3%),and impacting retinal surgery operation in 2 cases(2.9%).The main comorbidities were high myopia in 18 eyes(26.1%),trauma in 8 eyes(11.6%),retinal detachment in 6 eyes(8.7%),congenital cataracts in 8 eyes(11.6%),and Marfan's syndrome in 2 eyes(2.9%).The mean time from implantation to explantation was 4.0y [SD 4.2(0.005-15)].Treatment after explantation included posterior chamber IOL implantation in 44 eyes(63.8%) and aphakia in 25 eyes(36.2%).After surgery,the best corrected visual ability(BCVA) was improved in 50 cases(72.5%),including 28 patients(40.6%) in whom visual ability was improved by more than two lines.CONCLUSION:Dislocation/decentration is the main cause for explantation,and high myopia is a main risk factor.Posterior chamber IOL implantation remains the most elected treatment after explantation. 展开更多
关键词 explantation intraocular lens DISLOCATION
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Analysis for the dorsalization potency of the animal blastomeres of the 16 cell stage Xenopus embryo
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作者 Qinghua Tao Xiaoyan Ding 《Chinese Science Bulletin》 SCIE EI CAS 1999年第6期538-540,共3页
The derso-ventral axis of Xenopus embryo is established after fertilization. Blastula stage blastomeres acquire different identities as they have inherited different maternal materials which distribute radial symmetri... The derso-ventral axis of Xenopus embryo is established after fertilization. Blastula stage blastomeres acquire different identities as they have inherited different maternal materials which distribute radial symmetrically along the animal vegetal aixs in the full-grown oocyte, and are rearranged by the cortical rotation triggered by fertilization. The vegetal blastomeres demonstrate the different dorsalization potencies in the previous transplantation experiments. The data of blastomere explanting and RT-PCR analyzing indicate that the dorsal ventral bias also exists among the animal blastomeres even during the early blastula stage. 展开更多
关键词 XENOPUS embryo dorso-ventral axis BLASTOMERE explantation gene expression RT-PCR.
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Clinical Outcome for Failed Radial Head Arthroplasty Treated with Explantation
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作者 David C. Holt Aimee M. Struk +2 位作者 Joseph J. King Robert C. Matthias Thomas W. Wright 《Open Journal of Orthopedics》 2017年第8期199-210,共12页
Purpose: Failure after radial head arthroplasty is uncommon, but clinically significant. Treatment for failure may involve implant removal. We describe fourteen patients who underwent implant removal after failed radi... Purpose: Failure after radial head arthroplasty is uncommon, but clinically significant. Treatment for failure may involve implant removal. We describe fourteen patients who underwent implant removal after failed radial head arthroplasty. Methods: A retrospective review was performed to determine the cause of failure and clinical data were prospectively collected. Results: At mean follow up of 38 months the mean VAS for pain score was 3.0 and the mean DASH score was 40.5. The mean MEPS was 69. All patients demonstrated improved elbow range of motion. Mean elbow flexion at final follow up was 124&deg;to an average extension deficit of 25&deg;. Fifty percent of patients exhibited clinical symptoms of cubital tunnel syndrome. Three patients required additional surgery after implant removal. Conclusions: Implant removal for failed radial head arthroplasty improves range of motion and demonstrates acceptable outcomes at intermediate-term follow up. However, 50% of patients developed cubital tunnel syndrome and 21% required revision surgery. 展开更多
关键词 Clinical OUTCOME Cubital Tunnel Syndrome explantation IMPLANT Removal RADIAL Head ARTHROPLASTY
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Aspergillus infection of pacemaker in an immunocompetent host: a case report
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作者 Yuan GAO Rong-Fang LAN +3 位作者 Ning ZHANG Shao-Xian WANG Zhong-Lin HAN Wei XU 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2020年第1期58-60,共3页
The incidence of CIED(Cardiac Implantable Electronic Devices)infections is 0.13%–8.0%.[1,2]Fungal organisms account for up to 10%and Aspergillus infections are extremely rare.Here,we present a case of CIED-related As... The incidence of CIED(Cardiac Implantable Electronic Devices)infections is 0.13%–8.0%.[1,2]Fungal organisms account for up to 10%and Aspergillus infections are extremely rare.Here,we present a case of CIED-related Aspergillus infection in an immunocompetent patient. 展开更多
关键词 Aspergillus flavus Device explantation Pacemaker infection
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Sacral neuromodulation therapy of the lower urinary tract: A review of the literature and unanswered questions
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作者 Golnaz Alemi Vani Dandolu 《Open Journal of Obstetrics and Gynecology》 2013年第4期1-6,共6页
Objective: To review the evidence surrounding Sacral Neuromodulation therapy and delineate areas that will need more research. Methods: An extensive search was performed on the available literature on SNM for lower ur... Objective: To review the evidence surrounding Sacral Neuromodulation therapy and delineate areas that will need more research. Methods: An extensive search was performed on the available literature on SNM for lower urinary tract dysfunction. Based on the results of the search, the mechanisms of action, indications, technique, and patient characteristics of therapy failures and success are presented and discussed. Results: SNM is accepted by the FDA since 1997 for the treatment of lower urinary tract dysfunction. As it is a relatively new procedure, there are variations in the technique of lead placement, generator choice, testing interval, patient selection, time to explantation, and definitions of therapy failures and successes. Conclusions: SNM is a safe and therapeutic option for the treatment of urgencyfrequency syndrome, urge incontinence, and idiopathic urinary retention. However, there are multiple unanswered questions that require extensive research. 展开更多
关键词 SACRAL NEUROMODULATION SNM Peripheral Nerve Evaluation PNE InterStim Tined Lead Electrode Implantable Pulse Generator Lower URINARY Tract Dysfunction explantation URGENCY Frequency Syndrome URINARY Retention INCONTINENCE
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有晶状体眼人工晶状体植入术后二次手术的临床特征分析 被引量:2
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作者 李帅飞 游昌涛 +1 位作者 陈东栋 徐玲玲 《中华眼视光学与视觉科学杂志》 CAS CSCD 2022年第8期615-621,共7页
目的:探究有晶状体眼人工晶状体(ICL)植入术后需再次行人工晶状体调位、更换或取出术等二次手术的原因,分析其临床特点和术后效果。方法:系列病例研究。收集2017年7月至2020年12月在郑州爱尔眼科医院行ICL(V4c)植入术后行人工晶状体调... 目的:探究有晶状体眼人工晶状体(ICL)植入术后需再次行人工晶状体调位、更换或取出术等二次手术的原因,分析其临床特点和术后效果。方法:系列病例研究。收集2017年7月至2020年12月在郑州爱尔眼科医院行ICL(V4c)植入术后行人工晶状体调位、更换或取出术的屈光不正患者73例(74眼),其中需调位47眼、更换26眼、取出1眼,术后定期复查,观察记录裸眼视力(UCVA)、拱高和角膜内皮细胞计数(ECD)等指标。以单纯ICL植入术眼68眼作为对照组,ICL植入后行调位术眼47眼为调位组,ICL植入后行更换术眼26眼为更换组。采用独立样本t检验比较因拱高过高与拱高过低行二次手术处理患者的拱高变化量;采用单因素方差分析比较调位组、更换组与对照组在术后1个月的UCVA、有效性指数、安全性指数及ECD减少量的差异;采用卡方检验比较单纯球镜矫正型和复曲面型ICL植入术后的更换率。结果:ICL植入术后进行ICL调位、更换或取出的原因有拱高异常、复曲面型ICL旋转和ICL位置异常。因拱高异常行ICL调位或更换59眼,拱高过高者通过调位或更换小一号尺寸的ICL后均获得理想拱高,而拱高过低的病例相对复杂,且拱高过高者行ICL调位或更换的平均拱高变化量大于拱高过低者,差异有统计学意义(t=4.72,P<0.001)。复曲面型ICL植入术后发生轴向旋转且视觉质量欠佳14眼,其中12眼行调位后取得稳定、良好的效果,2眼调位后顽固性旋转,通过更换手术方式才解决。术后1个月,调位组、更换组与对照组的UCVA、有效性指数、安全性指数及ECD减少量比较差异均无统计学意义。单纯球镜矫正型ICL植入术后更换率小于复曲面型ICL(分别为1.6%、3.5%),差异有统计学意义(χ^(2)=4.04,P=0.045)。1眼ICL植入术后出现ICL位置异常、瞳孔区拱高上下差异大,行ICL取出+晶状体超声乳化吸除+囊袋内人工晶状体植入术,术后效果理� 展开更多
关键词 有晶状体眼人工晶状体植入术 调位 更换 取出
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人工晶状体取出术40例临床分析 被引量:2
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作者 冯星 施玉英 《首都医科大学学报》 CAS 2008年第5期653-655,共3页
目的分析人工晶状体取出术的指征、手术方法及预后。方法回顾性研究了39例患者40只眼,分析手术指征及预后。40只眼均通过前路手术取出人工晶状体,其中清亮角膜切口38例,巩膜隧道切口2例。取出同时植入人工晶状体的34例,未植入人工晶状体... 目的分析人工晶状体取出术的指征、手术方法及预后。方法回顾性研究了39例患者40只眼,分析手术指征及预后。40只眼均通过前路手术取出人工晶状体,其中清亮角膜切口38例,巩膜隧道切口2例。取出同时植入人工晶状体的34例,未植入人工晶状体的6例。结果人工晶状体取出的原因如下:人工晶状体脱位22例,葡萄膜炎9例,屈光不正4例,继发性青光眼2例,角膜水肿大泡2例,人工晶状体混浊1例。40例人工晶状体均一次手术成功取出,术后1个月26例矫正视力有明显提高。结论人工晶状体取出最常见的原因是人工晶状体脱位或半脱位、葡萄膜炎、屈光不正。及时正确的取出人工晶状体,可有效提高患者视力。根据患者实际情况,可同时植入适当的人工晶状体。 展开更多
关键词 人工晶状体 取出
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Indications for exchange or explantation of phakic implantable collamer lens with central port in patients with and without keratoconus 被引量:1
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作者 Albanderi Alhamzah Saad S.Alharbi +2 位作者 Fahad Alfardan Tariq Aldebasi Tariq Almudhaiyan 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2021年第11期1714-1720,共7页
AIM:To evaluate the causes of phakic implantable collamer lens(ICL)exchange/explantation in patients with and without keratoconus(KC)at two tertiary hospitals in Riyadh,Saudi Arabia.METHODS:A retrospective chart revie... AIM:To evaluate the causes of phakic implantable collamer lens(ICL)exchange/explantation in patients with and without keratoconus(KC)at two tertiary hospitals in Riyadh,Saudi Arabia.METHODS:A retrospective chart review of all patients who underwent ICL(model V4 c with central port)exchange/explantation was performed using the electronic medical record systems.All available preoperative and postoperative data were documented for each patient.RESULTS:Over 7 y,2283 ICL implantation procedures were performed;46 implants(2%)required exchange(21 implants)/explantation(25 implants),of which 14 cases(30.4%)were patients with KC.Indications for ICL exchange/explantation in non-KC group were vault measurement,cataract formation,increased intraocular pressure,inaccurate refraction,and patient dissatisfaction in 22(68.75%),4(12.5%),3(9.37%),2(6.25%),and 1(3.12%)case,respectively.The most common indication for ICL exchange/explantation in the KC group was inaccurate vault sizing in 11 patients(78.57%),inaccurate refraction in 2 patients(14.28%),and patient dissatisfaction postoperatively in 1(7.14%)case.CONCLUSION:ICL implantation results in predictable refractive outcomes over the long term with exchange/explantation rates comparable to previous literature.Improper vault size is the most common cause of ICL exchange/explantation among patients with or without KC. 展开更多
关键词 explantation EXCHANGE implantable collamer lens vault
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Inferior dermoglandular flap for autologous breast remodeling following explantation of breast implants in ptotic breasts:a case report and literature search
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作者 Umar Daraz Khan 《Plastic and Aesthetic Research》 2015年第1期81-84,共4页
Explantation following aesthetic mammoplasty without implant replacement is quite uncommon and often leaves the patient worse off than prior to mammoplasty.A case is presented here in which patient’s own tissue was u... Explantation following aesthetic mammoplasty without implant replacement is quite uncommon and often leaves the patient worse off than prior to mammoplasty.A case is presented here in which patient’s own tissue was used as an inferior dermoglandular flap for autologous breast remodeling.Inferior dermal flap has been described for breast reconstruction and simultaneous augmentation mammoplasty with mastopexy for prosthesis cover in the lower pole of the breast,but its use following explantation without implant replacement has not been described for breast remodeling and volume conservation. 展开更多
关键词 Autologous breast augmentation bostwick flap breast remodeling explantation revision augmentation mammoplasty
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大肠癌肿瘤相关成纤维细胞原代培养方法的优化 被引量:7
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作者 彭巍 徐小慧 +4 位作者 季江 苏玉飞 张钰明 吴永友 邢春根 《中华实验外科杂志》 CAS CSCD 北大核心 2013年第5期954-957,共4页
目的比较大肠癌肿瘤相关成纤维细胞(CCAFs)的不同原代培养方法,筛选最佳培养条件。方法取新鲜直肠癌标本,以高浓度双抗低温处理后进行原代培养;12d细胞计数法比较酶消化法与组织块植入法的CCAFs培养效率;利用6d贴壁率及12d爬出细... 目的比较大肠癌肿瘤相关成纤维细胞(CCAFs)的不同原代培养方法,筛选最佳培养条件。方法取新鲜直肠癌标本,以高浓度双抗低温处理后进行原代培养;12d细胞计数法比较酶消化法与组织块植入法的CCAFs培养效率;利用6d贴壁率及12d爬出细胞数法比较不同组织块大小(1、8mm^3)、培养基种类(1640、DMEM、F12)、培养瓶材质(玻璃瓶、塑料瓶)与血清浓度(10%、20%)的培养效率;所获CCAFs行免疫荧光鉴定并绘制生长曲线;以SPSS18.0软件进行统计学分析。结果组织块植入法优于酶消化法,12d细胞计数分别为(9.75±0.91)×10^4个与(1.83±1.22)×10^4个(t=13.733,P〈0.01);12d细胞计数法提示高血清浓度(t=-3.549,P〈0.01),较大组织块(t=-1.115,P〈0.05)可增加细胞培养效率;与F12和DMEM比较,1640培养基具有更高细胞培养效率和性价比,其回归方程为:Y=10.68750—3.96528X22-2.00694X,+1.03472X4;培养瓶材质不影响细胞数量(t=-0.116,P〉0.05);6d细胞爬出率与培养瓶和培养基有关,回归方程为:Y=0.81367+0.20478X.-0.33312X引原代培养的CCAFs表达α-平滑肌肌动蛋白(α—SMA)及波形蛋白(Vimentin)。结论高浓度双抗低温处理组织标本,以含高浓度胎牛血清的1640培养基,采用大组织块植入法是CCAFs原代培养的优化方法,获得的CCAFs具有典型肿瘤相关成纤维细胞(CAFs)特征。 展开更多
关键词 大肠癌相关肿瘤成纤维细胞 原代培养 培养条件 组织块植入法
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辽东楤木叶总皂苷(ETS)对裸鼠A549体外移植瘤抑制率及瘤体相关蛋白COX-2表达影响的实验研究 被引量:7
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作者 王丽岩 肖洪彬 +2 位作者 张秀萍 王鸣慧 李凌雁 《中医药学报》 CAS 2010年第6期30-32,共3页
目的:探讨ETS抗肿瘤作用及机制,为进一步将其开发成新靶点的抗肿瘤药物提供可靠的科学依据。方法:建立A549荷瘤裸鼠动物模型进行体内抑瘤实验,取瘤体,称瘤重,计算肿瘤抑制率;免疫组化二步法检测瘤体内相关蛋白COX-2,CD31抗体检测瘤体微... 目的:探讨ETS抗肿瘤作用及机制,为进一步将其开发成新靶点的抗肿瘤药物提供可靠的科学依据。方法:建立A549荷瘤裸鼠动物模型进行体内抑瘤实验,取瘤体,称瘤重,计算肿瘤抑制率;免疫组化二步法检测瘤体内相关蛋白COX-2,CD31抗体检测瘤体微血管密度。结果:ETS能明显抑制裸鼠A549体外移植瘤生长,抑制COX-2的表达,降低微血管密度。结论:研究结果为辽东楤木叶抗肿瘤机理提供了实验依据。 展开更多
关键词 辽东楤木叶总皂苷 移植瘤抑制率 蛋白COX-2 实验研究
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半关节移植治疗重复拇指畸形伴指间关节严重发育不良 被引量:6
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作者 朱晓光 胡春鹤 +1 位作者 张克亮 邵新中 《中华手外科杂志》 CSCD 北大核心 2006年第5期292-293,共2页
目的通过半关节移植修复缺如的指间关节近端关节面及侧副韧带,重建重复拇指畸形患者严重偏斜的指间关节。方法1994年3月—2002年11月间,对7例患者利用赘生拇指近节指骨的近端关节面及部分关节囊,行一期或二期半关节移植,重建保留拇指的... 目的通过半关节移植修复缺如的指间关节近端关节面及侧副韧带,重建重复拇指畸形患者严重偏斜的指间关节。方法1994年3月—2002年11月间,对7例患者利用赘生拇指近节指骨的近端关节面及部分关节囊,行一期或二期半关节移植,重建保留拇指的指间关节近端发育不良或缺如的关节面。术后随访3年,观察骨关节生长及功能恢复情况。结果移植的半关节大部存活,85%患者的骨外形良好、功能恢复较满意。结论半关节移植是治疗重复拇指畸形伴指间关节严重发育不良的一种可行的手术方法。 展开更多
关键词 外科手术 复拇指畸形 指间关节 半关节移植
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后房型人工晶状体脱位的巩膜缝线固定置换术的临床应用
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作者 赵慧玲 葛娴 +1 位作者 许哲 徐雯 《中国眼耳鼻喉科杂志》 2024年第4期268-272,共5页
目的通过对巩膜缝线固定置换后房型人工晶状体(IOL)手术治疗中远期IOL脱位的手术前后比较,评价术式的临床疗效。方法回顾性病例研究。纳入2022年3月—2024年3月期间于浙江大学医学院附属第二医院行手术治疗的中远期IOL脱位患者19例(23... 目的通过对巩膜缝线固定置换后房型人工晶状体(IOL)手术治疗中远期IOL脱位的手术前后比较,评价术式的临床疗效。方法回顾性病例研究。纳入2022年3月—2024年3月期间于浙江大学医学院附属第二医院行手术治疗的中远期IOL脱位患者19例(23眼),行巩膜缝线固定置换后房型IOL手术治疗。统计患者手术前后的裸眼视力(UCVA)、最佳矫正视力(BCVA)、眼压(IOP)、角膜内皮计数和术后并发症的发生率。结果经过1个月~1年的随访,患者术后的UCVA、BCVA均较术前提高(P<0.05),IOP较术前差异无统计学意义(P>0.05),无相关严重并发症。结论巩膜缝线固定置换后房型IOL手术对中远期IOL脱位具有较好的临床疗效。 展开更多
关键词 人工晶状体脱位 人工晶状体取出 巩膜缝线固定
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人脐带间充质干细胞分离培养方法的优化 被引量:2
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作者 张亚倩 梁军 +2 位作者 田沁森 丁玉芳 余道海 《牡丹江医学院学报》 2013年第5期7-9,共3页
目的:优化人脐带间充质干细胞(umbilical cord mesenchymal stem cells,UC-MSCs)分离培养的方法。方法:无菌条件下剔除剖腹产胎儿脐带动静脉,用牙剪子剪为约1mm3但彼此相连的组织块,得到贴壁细胞,采用半量换液进行原代及传代培养。用流... 目的:优化人脐带间充质干细胞(umbilical cord mesenchymal stem cells,UC-MSCs)分离培养的方法。方法:无菌条件下剔除剖腹产胎儿脐带动静脉,用牙剪子剪为约1mm3但彼此相连的组织块,得到贴壁细胞,采用半量换液进行原代及传代培养。用流式细胞仪检测其免疫表型。结果:成功从人脐带中分离纯化得到UC-MSCs,呈条形和成纤维细胞样的两种形态。UC-MSCs高度表达CD105、CD73、CD44、CD90,极少数表达CD45、CD34、CD14和HLA-DR。结论:建立出一种快捷经济的UC-MSCs体外分离培养方法。 展开更多
关键词 间充质干细胞 分离培养 植块法 脐带
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37例甲床损伤的显微修复治疗分析 被引量:2
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作者 欧春培 利春叶 +1 位作者 杨钦泰 李晓文 《中国现代医生》 2009年第12期59-59,61,F0003,共3页
目的探讨甲床损伤的治疗方法及其疗效。方法对2006年来以来收治的37例甲床损伤并发原甲板缺失患者的疗效进行回顾,总结分析其治疗方法。结果不同的病例采用不同的方法综合治疗,结果疗效满意。结论甲床损伤并发原甲板缺失的病例要积极微... 目的探讨甲床损伤的治疗方法及其疗效。方法对2006年来以来收治的37例甲床损伤并发原甲板缺失患者的疗效进行回顾,总结分析其治疗方法。结果不同的病例采用不同的方法综合治疗,结果疗效满意。结论甲床损伤并发原甲板缺失的病例要积极微创治疗,才能收到满意的疗效。 展开更多
关键词 甲床损伤 修复 甲板 回植
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人工晶状体取出术68只眼临床分析 被引量:2
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作者 楚美芳 柴芳 +1 位作者 张倩 马波 《中国实用眼科杂志》 2016年第1期65-68,共4页
目的通过对人工晶状体取出病例的原因分析,探讨人工晶状体植入手术的适应证及并发症的防治。方法回顾在西安市第四医院眼科自2013年1月至2015年1月行人工晶状体取出术共65例68只眼进行临床分析。结果导致人工晶状体取出的常见原因依次... 目的通过对人工晶状体取出病例的原因分析,探讨人工晶状体植入手术的适应证及并发症的防治。方法回顾在西安市第四医院眼科自2013年1月至2015年1月行人工晶状体取出术共65例68只眼进行临床分析。结果导致人工晶状体取出的常见原因依次为人工晶状体脱位或半脱位、视网膜脱离、人工晶状体度数误差、眼内炎等。人工晶状体植入至取出间隔时间最短1d,最长为15年,人工晶状体植人多在术后1月内和2~5年发生严重并发症导致人工晶状体取出。取出的人工晶状体:后房型64只眼,ICL3只眼,前房型1只眼。术后视力:53只眼(77.9%)有不同程度的提高,14只眼(20.6%)无明显变化,1只眼因并发眼内炎,术后视力由光感变为无光感。结论人工晶状体植入术后的严重并发症是导致人工晶状体取出的直接原因。术前严格掌握人工晶状体植入的适应证,有效控制术中、术后的并发症,是避免术后人工晶状体取出的重要保证,及时准确地取出人工晶状体,同时酌情植入适当的人工晶状体,可有效提高患者视力和生活质量。 展开更多
关键词 白内障 人工晶状体取出 并发症
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植块法分离培养人脐带间充质干细胞 被引量:1
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作者 胡文龙 杜玉丹 +3 位作者 王丽丽 汪建样 耿书国 殷嫦嫦 《九江学院学报(自然科学版)》 CAS 2015年第4期96-100,共5页
目的 探讨体外植块法分离人脐带间充质干细胞的可行性,为体外获取纯度较高的人间充质干细胞提供理论基础。方法 采用植块法分离培养人脐带间充质干细胞,在倒置相差显微镜下观察细胞的黏附、增殖及形态学的变化,并采用CCK-8法绘制P3代细... 目的 探讨体外植块法分离人脐带间充质干细胞的可行性,为体外获取纯度较高的人间充质干细胞提供理论基础。方法 采用植块法分离培养人脐带间充质干细胞,在倒置相差显微镜下观察细胞的黏附、增殖及形态学的变化,并采用CCK-8法绘制P3代细胞生长曲线。结果 脐带组织贴壁培养6~10d即可从组织块边缘爬出的成纤维样细胞,传代后,细胞均呈长梭形,以栅栏样、漩涡样及鱼群样贴壁生长,细胞间紧密排列,细胞生长曲线呈近似S型。结论 植块法能从人脐带组织中简便高效地分离出高纯度、高增殖活性的人脐带间充质干细胞。 展开更多
关键词 人脐带间充质干细胞 植块法 分离培养
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Foldable Lens Explantation and Exchange: The Reason and Solution 被引量:1
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作者 Danying Zheng , Zhenpin Zhang , Wenhui Yang , Weirong ChenZhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences , Guangzhou, 510060, China 《Eye Science》 CAS 2001年第1期54-56,共3页
Objective: To report the explantation and exchange of Hydrophilic Acrylic foldable intraocular lens (IOL) on 14 patients who had visual disturbances caused by the change of transparence on optic. Methods: Sixteen Hydr... Objective: To report the explantation and exchange of Hydrophilic Acrylic foldable intraocular lens (IOL) on 14 patients who had visual disturbances caused by the change of transparence on optic. Methods: Sixteen Hydrophilic Acrylic foldable intraocular lenses from 14 patients who presented with decreased visual acuity from 6 months to 1 year after normal phacoemulsification and IOL implantation associated with extensive transparent change on optic of the lens. The lenses were explanted with the bisection technique. All the eyes were reinserted with Acrysof foldable lenses. Results: Sixteen lenses were removed successfully and exchanged with the new lens in the capsule. The posterior capsular rupture and vitreous loss were found in the first two cases. One of them had the zonulysis due to the radial tear of the anterior capsule during the enlargement of the capsular opening. The anterior vitrectomy was performed with IOL fixed on the ciliary sulcus. The visual acuity of all the patients improved obviously without posterior complication. Conclusion: Foldable lens explantation with the bisection technique and exchange had a successful outcome with improvement of ocular condition. Eye science 2001; 17:54 ~56. 展开更多
关键词 折叠透镜 亲水性丙烯酸 视觉障碍 IOL 透明度
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