Background For patients with end-stage hepatic alveolar echinococcosis （AE）, in vivo resection of the involved parts of the liver is usually very difficult, therefore, allogenic liver transplantation is indicated. H...Background For patients with end-stage hepatic alveolar echinococcosis （AE）, in vivo resection of the involved parts of the liver is usually very difficult, therefore, allogenic liver transplantation is indicated. However, we hypothesize that for selected patents, ex vivo liver resection for thorough elimination of the involved tissues and liver autotransplantation may offer a chance for clinical cure. Methods We presented a 24-year-old women with a giant hepatic AE lesion who was treated with hepatectomy, ex vivo resection of the involved tissue and hepatic autotransplantation. The patient had moderate jaundice and advanced hepatic AE lesion which involved segments I, IV, V, VI, VII, VIII and retrohepatic inferior vena cava. The lateral segments （II and III） of the left liver remained normal with over 1000 ml in its volume. No extrahepatic metastases （such as to the lung or brain） could be found. As the first step of treatment, X-ray guided percutaneous transhepatic cholangiodrainage （PTCD） was performed twice for bile drainage in segment III and II separately until her serum total bilirubin decreased gradually from 236 to 88 umol/L. Total liver resection was then performed, followed by extended right hepatic trisegmentectomy and the entire retrohepatic vena cava was surgically removed en bloc while her hemodynamics parameters were stable. Neither veino-veinous bypass nor temporary intracorporeal cavo-caval or porto-caval shunt was used during the 5.7-hour anhepatic phase. The remained AE-free lateral segments of the-left liver were re-implanted in situ. The left hepatic vein was directly anastomosed end-to-end to the suprahepatic inferior vena cava due to the lack of the retrohepatic inferior vena cava with AE total infiltration. Because compensatory retroperitoneal porto-caval collateral circulation developed, we enclosed remained infrahepatic inferior vena cava at renal vein level without any haemodynamics problems. Results During a 60-day following-up after operation, the patient had a good r展开更多
研究了不同浓度纳他霉素对冬枣浆胞病菌Alternaria Nees ex Wallr．的室内毒力以及对冬枣低温贮藏期间生理生化指标的影响。结果表明，纳他霉素对冬枣浆胞病菌的EC50和EC90值分别为39．56和208．48mg／L，与对照药剂咪鲜胺、异菌脲相比...研究了不同浓度纳他霉素对冬枣浆胞病菌Alternaria Nees ex Wallr．的室内毒力以及对冬枣低温贮藏期间生理生化指标的影响。结果表明，纳他霉素对冬枣浆胞病菌的EC50和EC90值分别为39．56和208．48mg／L，与对照药剂咪鲜胺、异菌脲相比，EC90值无显著差异；经500～1000mg／L的纳他霉素处理可显著抑制冬枣丙二醛（MDA）含量的增加，并使多聚半乳糖醛酸酶（PG）与苯丙氨酸解氨酶（PAL）的活性提高，对保持果实硬度有很好的效果，并且抑制了可溶性固形物、抗坏血酸（Vc）含量的下降。经1000mg／L纳他霉素处理的冬枣贮藏到60d其商品率迭87．3％，高于对照组的商品率（72．9％）；贮藏到100d其商品率仍可达到75．0％，显著高于对照组（50．2％）。展开更多
Abstract: Ganoderma lucidum (Leyss. ex Fr.) Karst., a medicinal fungus called “Lingzhi” in China, has been used in traditional Chinese medicine in China for the prevention and treatment of various types of diseases,...Abstract: Ganoderma lucidum (Leyss. ex Fr.) Karst., a medicinal fungus called “Lingzhi” in China, has been used in traditional Chinese medicine in China for the prevention and treatment of various types of diseases, such as cancer, hepatopathy, arthritis, hypertension, neurasthenia, and chronic hepatitis. It is clear that the anticancer activity of G. lucidum is mainly due to polysaccharides and/or triterpenoids of the fungus. However, until now, the mechanism of the anticancer action of G. lucidum has not been well understood and, previously, the activation of the immune response of the host was widely considered to be the only mechanism by which G. lucidum prevented and/or treated cancer. However, recent studies reviewed in the present paper have shown that the potential mechanisms of anticancer action include not only the activation of the immune response of the host, but also the induction of cell differentiation, the induction of Phase II-metabolizing enzymes, the inhibition of angiogenesis, and the inhibition of the expression of the urokinase-type plasminogen activator (uPA) and the uPA receptor in cancer cells. To further elucidate the mechanisms of action of G. lucidum, more in vivo tests and randomized controlled clinical trials should be carried out, and the molecular mechanisms should be studied intensively. Additionally, whether the anticancer compounds in G. lucidum act synergistically or independently should be further studied.展开更多
Objective: To explore the possibility of using traditional Chinese medicine (TCM) in treating β thalassemia, its clinical effect and molecular mechanism of the action.Methods: According to the TCM theory of“Shen pro...Objective: To explore the possibility of using traditional Chinese medicine (TCM) in treating β thalassemia, its clinical effect and molecular mechanism of the action.Methods: According to the TCM theory of“Shen producing marrow”, the composite recipe, Yisui Shenxueling Granule (YSSXL), consisting of Chinese drugs for nourishing Shen and supplementing marrow (NS&SM) was given orally to 7 8 patients with β thalassemia (49 of the severe type and 29 of moderate type ), 3 times a day, 10 g each time (for children, the dose would be reduced proper ly), with 3 months as one therapeutic course, and no blood transfusion used in t he course. The clinical therapeutic efficacy and hematologic parameters in patie nts were observed, and systemic gene analysis was conducted with PAGE, PCR, PCR SSCP, RT PCR and DNA sequences analysis and mRNA detection, in order to s tudy the molecular mechanism from the relationships between genetic mutation and clinical efficacy, gene expression and its regulation. Results: YSSXL showed obvious therapeutic effect in treating β thalassemia. Gene analysis revealed that it did not change the genetic mutatio n type, but could obviously increase hemoglobin, fetal hemoglobin (HbF), γ/(β+ γ) globin ratio, γ globin mRNA expression and GM CSF mRNA expression in patients, as well as the GM CSFmRMA in marrow of mice after 60 Co radia tion. Conclusion: YSSXL has a remarkable therapeutic effect on β tha lassemia, and its possible mechanism is its action in unlocking γ gene, in creasing the γ globin expression and enhancing HbF synthesis so as to compe nsate for the gene defect. This study has opened a new path for the treatment of β thalassemia with TCM.展开更多
蒜头果(Malania oleifera Chun et S.Lee ex S.Lee)为中国特有的单种属极小种群稀有濒危植物,仅桂、滇少数县市有分布,其作为木本油料树种开发应用价值极高。文中对当前蒜头果的资源分布及数量、开发利用价值、育苗及造林情况和濒危原...蒜头果(Malania oleifera Chun et S.Lee ex S.Lee)为中国特有的单种属极小种群稀有濒危植物,仅桂、滇少数县市有分布,其作为木本油料树种开发应用价值极高。文中对当前蒜头果的资源分布及数量、开发利用价值、育苗及造林情况和濒危原因等进行了简要的概述和分析,并从资源保存和育种的角度,提出了一些研究思路,以期为蒜头果种质资源保存和良种选育提供参考。展开更多
We review achievements in the conservation of orchid diversity in China over the last 21 years.We provide updated information on orchid biodiversity and suggestions for orchid conservation in China.We outline national...We review achievements in the conservation of orchid diversity in China over the last 21 years.We provide updated information on orchid biodiversity and suggestions for orchid conservation in China.We outline national policies of biodiversity conservation,especially of orchid conservation,which provide general guidelines for orchid conservation in China.There are now approximately 1708 known species of Orchidaceae in 181 genera in China,including five new genera and 365 new species described over the last 21 years.The assessment of risk of extinction of all 1502 known native orchid species in China in 2013 indicated that 653 species were identified as threatened,132 species were treated as data-deficient,and four species endemic to China were classified as extinct.Approximately 1100 species(ca.65%)are protected in national nature reserves,and another~66 species in provincial nature reserves.About 800 native orchid species have living collections in major botanical gardens.The pollination biology of 74 native orchid species and the genetic diversity and spatial genetic structure of 29 orchid species have been investigated at a local scale and/or across species distributions.The mycorrhizal fungal community composition has been investigated in many genera,such as Bletilla,Coelogyne,Cymbidium,Cypripedium,and Dendrobium.Approximately 292 species will be included in the list of national key protected wild plants this year.Two major tasks for near future include in situ conservation and monitoring population dynamics of endangered species.展开更多
CRISPR/Cas9 is a versatile genome-editing tool which is widely used for modifying the genome of both prokaryotic and eukaryotic organisms for basic research and applications. An increasing number of reports have demon...CRISPR/Cas9 is a versatile genome-editing tool which is widely used for modifying the genome of both prokaryotic and eukaryotic organisms for basic research and applications. An increasing number of reports have demonstrated that CRISPR/Cas9-mediated genome editing is a powerful technology for gene therapy. Here, we review the recent advances in CRISPR/Cas9-mediated gene therapy in animal models via different strategies and discuss the challenges as well as future prospects.展开更多
The effectiveness of liver autotransplantation for patients with partial hepatic alveolar echinococcosis was analyzed.We retrospectively studied 6 patients with hepatic alveolar echinococcosis who underwent liver auto...The effectiveness of liver autotransplantation for patients with partial hepatic alveolar echinococcosis was analyzed.We retrospectively studied 6 patients with hepatic alveolar echinococcosis who underwent liver autotransplantation in our hospital from 2008 to 2010.We also summarized the surgical indications of liver autotransplantation for hepatic alveolar echinococcosis and our experience in the management of postoperative complications of liver autotransplantation.Of 6 patients,5 achieved good curative results,and one died of multiple organ failure caused by portal vein thrombosis.Main complications included postoperative bleeding,bile leak and small-for-size liver graft syndrome.Liver autotransplantation offers a new approach to cure hepatic alveolar echinococcosis with non-resectable lesions.It could be the most effective method to cure intractable hepatic alveolar echinococcosis if correct handling in operation and proper prevention of complications are performed.But the long-term outcomes are still needed to be confirmed in longer follow-up.展开更多
Root-knot nematodes（RKNs,Meloidogyne spp.） are obligate biotrophic parasites that settle close to the vascular tissues in plant roots.The diseases resulting from RKN infections cause serious damage to agricultural p...Root-knot nematodes（RKNs,Meloidogyne spp.） are obligate biotrophic parasites that settle close to the vascular tissues in plant roots.The diseases resulting from RKN infections cause serious damage to agricultural production worldwide.In the present paper,the resistance of Chinese leek（Allium tuberosum Rottler ex Sprengel） against RKNs,its suppressive effect on nematode disease,its nematicidal activity and its component profile were studied to identify an efficient disease control method.In soil heavily infected by nematodes,Chinese leek showed strong resistance to RKNs.Additionally,the gall indexes of cucumber plants rotated with Chinese leek and of tomato plants intercropped with Chinese leek were reduced by 70.2 and 41.1%,respectively.In a pot experiment,the gall indexes of Chinese leek extract-treated tomato and cucumber plants were reduced by 88.9 and 75.9%,respectively.In an in vitro experiment,the mortality rate of a RKN（Meloidogyne incognita J2） treated with Chinese leek extract was significantly higher than that of the control.The gas chromatography-mass spectrometry（GC-MS） analysis revealed that glycosides,carboxylic acids,ketones and organic sulfides are the main components in the Chinese leek extract.This study revealed that Chinese leek possesses a high resistance to RKNs,has strong nematicidal activity against M.incognita and can significantly reduce the incidence of disease caused by nematodes.展开更多
Hepatitis C virus(HCV) is a major cause of liver diseases including liver cirrhosis and hepatocellular carcinoma. Approximately 3% of the world population is infected with HCV. Thus, HCV infection is considered a publ...Hepatitis C virus(HCV) is a major cause of liver diseases including liver cirrhosis and hepatocellular carcinoma. Approximately 3% of the world population is infected with HCV. Thus, HCV infection is considered a public healthy challenge. It is worth mentioning, that the HCV prevalence is dependent on the countries with infection rates around 20% in high endemic countries. The review summarizes recent data on HCV molecular biology, the physiopathology of infection(immune-mediated liver damage, liver fibrosis and lipid metabolism), virus diagnostic and treatment. In addition, currently available in vitro, ex vivo and animal models to study the virus life cycle, virus pathogenesis and therapy are described. Understanding of both host and viral factors may in the future lead to creation of new approaches in generation of an efficient therapeutic vaccine.展开更多
Catalytic fast pyrolysis （CFP） is deemed as the most promising way to convert biomass to transportation fuels or value added chemicals. Most works in literature so far have focused on the in situ CFP where the catal...Catalytic fast pyrolysis （CFP） is deemed as the most promising way to convert biomass to transportation fuels or value added chemicals. Most works in literature so far have focused on the in situ CFP where the catalysts are packed or co-fed with the feedstock in the pyrolysis reactor. However, the ex situ CFP with catalysts separated from the pyrolyzer has attracted more and more attentions due to its unique advantages of individually optimizing the pyrolysis conditions and catalyst performances. This review compares the differences between the in situ and ex situ CFP operation, and summarizes the development and progress of ex situ CFP applications, including the rationale and performances of different catalysts, and the choices of suitable ex situ reactor systems. Due to the complex composition of bio-oil, no single approach was believed to be able to solve the problems completely among all those existing technologies. With the increased understanding of catalyst performances and reaction process, the recent trend toward an integration of biomass or bio-oil fractionation with subsequent thermo/biochemical conversion routes is also discussed.展开更多
OBJECTIVE: To evaluate the clinical effect of traditional Chinese spinal orthopedic manipulation(TCSOM) in treating chondromalacia patellae(CP).METHODS: Sixty cases of CP patients were randomly assigned to a TCSOM gro...OBJECTIVE: To evaluate the clinical effect of traditional Chinese spinal orthopedic manipulation(TCSOM) in treating chondromalacia patellae(CP).METHODS: Sixty cases of CP patients were randomly assigned to a TCSOM group and a Celecoxib group according to the random number table method. All patients in the TCSOM group were treated with a maximum of 10 spinal manipulations and rehabilitation training of quadriceps femoris. The symptoms before and after treatment were assessed with visual analog scale(VAS) and Kujala functional knee scoring system(KFKSS). A symptom improvement rate(SIR) was implemented in order to evaluate the effects of the treatments.RESULTS: The symptoms of 16 patients in the TC-SOM group quickly resolved after the first spinal manipulation and 8 cases were significantly improved. The VAS scores in the TCSOM group after 4weeks of treatment were significantly lower than those in the Celecoxib group. The KFKSS scores in the TCSOM group after 4 weeks of treatment were significantly higher than those in the Celecoxib group. Side effects of the treatment were not reported. Symptom improvement rate based on the VAS in the TCSOM group indicated more significant improvements than the Celecoxib group.CONCLUSION: TCSOM has greater efficacy than Celecoxib capsules for relief of the symptoms of CP.展开更多
Spontaneous hybridization in ex situ facilities can undermine the genetic integrity of ex situ collections and potentially contaminate open-pollinated seeds or seedlings destined for the reintroduction of endangered p...Spontaneous hybridization in ex situ facilities can undermine the genetic integrity of ex situ collections and potentially contaminate open-pollinated seeds or seedlings destined for the reintroduction of endangered plant species into the wild. In the present study, the potential risk of hybridization between two endangered Chinese endemic species, namely Sinojackia xylocarpa Hu and S. rehderiana Hu, which are naturally allopatric species but were conserved ex situ in Wuhan Botanical Garden （WBG）, Wuhan, China, were investigated over three consecutive years from 2003 to 2005. The entire overlapping flowering period of the two species was 14-20 d and the two species shared the same pollinator insects during the entire flowering season in WBG. The floral isolation between the two species was not an issue in the ex sltu collection at WBG. The results suggest an opportunity for pollen transfer between species and a potential risk of genetic Introgression and loss of genetic identity of open-pollinated seeds produced in the ex sltu Collection of these two endangered species. An artificial reciprocal cross between S xylocarpa and S. rehderlana confirmed that the two congener species could readily set seeds, indicating no post-pollination barriers to hybridization and the importance of spatial isolation as a barrier to inter-specific crossing. Therefore, to manage these crossable species with overlapping flowering times and shared pollination vectors in ex situ facilities, spatial isolation should be carefully considered to minimize the possibility of spontaneous hybridization.展开更多
文摘Background For patients with end-stage hepatic alveolar echinococcosis （AE）, in vivo resection of the involved parts of the liver is usually very difficult, therefore, allogenic liver transplantation is indicated. However, we hypothesize that for selected patents, ex vivo liver resection for thorough elimination of the involved tissues and liver autotransplantation may offer a chance for clinical cure. Methods We presented a 24-year-old women with a giant hepatic AE lesion who was treated with hepatectomy, ex vivo resection of the involved tissue and hepatic autotransplantation. The patient had moderate jaundice and advanced hepatic AE lesion which involved segments I, IV, V, VI, VII, VIII and retrohepatic inferior vena cava. The lateral segments （II and III） of the left liver remained normal with over 1000 ml in its volume. No extrahepatic metastases （such as to the lung or brain） could be found. As the first step of treatment, X-ray guided percutaneous transhepatic cholangiodrainage （PTCD） was performed twice for bile drainage in segment III and II separately until her serum total bilirubin decreased gradually from 236 to 88 umol/L. Total liver resection was then performed, followed by extended right hepatic trisegmentectomy and the entire retrohepatic vena cava was surgically removed en bloc while her hemodynamics parameters were stable. Neither veino-veinous bypass nor temporary intracorporeal cavo-caval or porto-caval shunt was used during the 5.7-hour anhepatic phase. The remained AE-free lateral segments of the-left liver were re-implanted in situ. The left hepatic vein was directly anastomosed end-to-end to the suprahepatic inferior vena cava due to the lack of the retrohepatic inferior vena cava with AE total infiltration. Because compensatory retroperitoneal porto-caval collateral circulation developed, we enclosed remained infrahepatic inferior vena cava at renal vein level without any haemodynamics problems. Results During a 60-day following-up after operation, the patient had a good r
文摘研究了不同浓度纳他霉素对冬枣浆胞病菌Alternaria Nees ex Wallr．的室内毒力以及对冬枣低温贮藏期间生理生化指标的影响。结果表明，纳他霉素对冬枣浆胞病菌的EC50和EC90值分别为39．56和208．48mg／L，与对照药剂咪鲜胺、异菌脲相比，EC90值无显著差异；经500～1000mg／L的纳他霉素处理可显著抑制冬枣丙二醛（MDA）含量的增加，并使多聚半乳糖醛酸酶（PG）与苯丙氨酸解氨酶（PAL）的活性提高，对保持果实硬度有很好的效果，并且抑制了可溶性固形物、抗坏血酸（Vc）含量的下降。经1000mg／L纳他霉素处理的冬枣贮藏到60d其商品率迭87．3％，高于对照组的商品率（72．9％）；贮藏到100d其商品率仍可达到75．0％，显著高于对照组（50．2％）。
文摘Abstract: Ganoderma lucidum (Leyss. ex Fr.) Karst., a medicinal fungus called “Lingzhi” in China, has been used in traditional Chinese medicine in China for the prevention and treatment of various types of diseases, such as cancer, hepatopathy, arthritis, hypertension, neurasthenia, and chronic hepatitis. It is clear that the anticancer activity of G. lucidum is mainly due to polysaccharides and/or triterpenoids of the fungus. However, until now, the mechanism of the anticancer action of G. lucidum has not been well understood and, previously, the activation of the immune response of the host was widely considered to be the only mechanism by which G. lucidum prevented and/or treated cancer. However, recent studies reviewed in the present paper have shown that the potential mechanisms of anticancer action include not only the activation of the immune response of the host, but also the induction of cell differentiation, the induction of Phase II-metabolizing enzymes, the inhibition of angiogenesis, and the inhibition of the expression of the urokinase-type plasminogen activator (uPA) and the uPA receptor in cancer cells. To further elucidate the mechanisms of action of G. lucidum, more in vivo tests and randomized controlled clinical trials should be carried out, and the molecular mechanisms should be studied intensively. Additionally, whether the anticancer compounds in G. lucidum act synergistically or independently should be further studied.
基金This item was supported by National Funds of Natural Sciences (No. 3017119 9) and Natural Science Foundation of Guangxi Autonomous Region (No. 014402C)
文摘Objective: To explore the possibility of using traditional Chinese medicine (TCM) in treating β thalassemia, its clinical effect and molecular mechanism of the action.Methods: According to the TCM theory of“Shen producing marrow”, the composite recipe, Yisui Shenxueling Granule (YSSXL), consisting of Chinese drugs for nourishing Shen and supplementing marrow (NS&SM) was given orally to 7 8 patients with β thalassemia (49 of the severe type and 29 of moderate type ), 3 times a day, 10 g each time (for children, the dose would be reduced proper ly), with 3 months as one therapeutic course, and no blood transfusion used in t he course. The clinical therapeutic efficacy and hematologic parameters in patie nts were observed, and systemic gene analysis was conducted with PAGE, PCR, PCR SSCP, RT PCR and DNA sequences analysis and mRNA detection, in order to s tudy the molecular mechanism from the relationships between genetic mutation and clinical efficacy, gene expression and its regulation. Results: YSSXL showed obvious therapeutic effect in treating β thalassemia. Gene analysis revealed that it did not change the genetic mutatio n type, but could obviously increase hemoglobin, fetal hemoglobin (HbF), γ/(β+ γ) globin ratio, γ globin mRNA expression and GM CSF mRNA expression in patients, as well as the GM CSFmRMA in marrow of mice after 60 Co radia tion. Conclusion: YSSXL has a remarkable therapeutic effect on β tha lassemia, and its possible mechanism is its action in unlocking γ gene, in creasing the γ globin expression and enhancing HbF synthesis so as to compe nsate for the gene defect. This study has opened a new path for the treatment of β thalassemia with TCM.
文摘蒜头果(Malania oleifera Chun et S.Lee ex S.Lee)为中国特有的单种属极小种群稀有濒危植物,仅桂、滇少数县市有分布,其作为木本油料树种开发应用价值极高。文中对当前蒜头果的资源分布及数量、开发利用价值、育苗及造林情况和濒危原因等进行了简要的概述和分析,并从资源保存和育种的角度,提出了一些研究思路,以期为蒜头果种质资源保存和良种选育提供参考。
基金supported by Grants from National Forestry and Grassland Administration,China(No.2019073018,2019073019)National Natural Science Foundation of China(No.31870195,31670194)。
文摘We review achievements in the conservation of orchid diversity in China over the last 21 years.We provide updated information on orchid biodiversity and suggestions for orchid conservation in China.We outline national policies of biodiversity conservation,especially of orchid conservation,which provide general guidelines for orchid conservation in China.There are now approximately 1708 known species of Orchidaceae in 181 genera in China,including five new genera and 365 new species described over the last 21 years.The assessment of risk of extinction of all 1502 known native orchid species in China in 2013 indicated that 653 species were identified as threatened,132 species were treated as data-deficient,and four species endemic to China were classified as extinct.Approximately 1100 species(ca.65%)are protected in national nature reserves,and another~66 species in provincial nature reserves.About 800 native orchid species have living collections in major botanical gardens.The pollination biology of 74 native orchid species and the genetic diversity and spatial genetic structure of 29 orchid species have been investigated at a local scale and/or across species distributions.The mycorrhizal fungal community composition has been investigated in many genera,such as Bletilla,Coelogyne,Cymbidium,Cypripedium,and Dendrobium.Approximately 292 species will be included in the list of national key protected wild plants this year.Two major tasks for near future include in situ conservation and monitoring population dynamics of endangered species.
基金supported by the National Natural Science Foundation of China (31371455, 31171318 to Dali Li, 81330049 to Mingyao Liu)the Science and Technology Commission of Shanghai Municipality (14140900300 to Dali Li)
文摘CRISPR/Cas9 is a versatile genome-editing tool which is widely used for modifying the genome of both prokaryotic and eukaryotic organisms for basic research and applications. An increasing number of reports have demonstrated that CRISPR/Cas9-mediated genome editing is a powerful technology for gene therapy. Here, we review the recent advances in CRISPR/Cas9-mediated gene therapy in animal models via different strategies and discuss the challenges as well as future prospects.
文摘The effectiveness of liver autotransplantation for patients with partial hepatic alveolar echinococcosis was analyzed.We retrospectively studied 6 patients with hepatic alveolar echinococcosis who underwent liver autotransplantation in our hospital from 2008 to 2010.We also summarized the surgical indications of liver autotransplantation for hepatic alveolar echinococcosis and our experience in the management of postoperative complications of liver autotransplantation.Of 6 patients,5 achieved good curative results,and one died of multiple organ failure caused by portal vein thrombosis.Main complications included postoperative bleeding,bile leak and small-for-size liver graft syndrome.Liver autotransplantation offers a new approach to cure hepatic alveolar echinococcosis with non-resectable lesions.It could be the most effective method to cure intractable hepatic alveolar echinococcosis if correct handling in operation and proper prevention of complications are performed.But the long-term outcomes are still needed to be confirmed in longer follow-up.
基金supported by the National Natural Science Foundation of China(31272151,31471864)the Agro-scientific Research in the Public Interest,China(201103018)the China Agriculture Research System(CARS-25)
文摘Root-knot nematodes（RKNs,Meloidogyne spp.） are obligate biotrophic parasites that settle close to the vascular tissues in plant roots.The diseases resulting from RKN infections cause serious damage to agricultural production worldwide.In the present paper,the resistance of Chinese leek（Allium tuberosum Rottler ex Sprengel） against RKNs,its suppressive effect on nematode disease,its nematicidal activity and its component profile were studied to identify an efficient disease control method.In soil heavily infected by nematodes,Chinese leek showed strong resistance to RKNs.Additionally,the gall indexes of cucumber plants rotated with Chinese leek and of tomato plants intercropped with Chinese leek were reduced by 70.2 and 41.1%,respectively.In a pot experiment,the gall indexes of Chinese leek extract-treated tomato and cucumber plants were reduced by 88.9 and 75.9%,respectively.In an in vitro experiment,the mortality rate of a RKN（Meloidogyne incognita J2） treated with Chinese leek extract was significantly higher than that of the control.The gas chromatography-mass spectrometry（GC-MS） analysis revealed that glycosides,carboxylic acids,ketones and organic sulfides are the main components in the Chinese leek extract.This study revealed that Chinese leek possesses a high resistance to RKNs,has strong nematicidal activity against M.incognita and can significantly reduce the incidence of disease caused by nematodes.
文摘Hepatitis C virus(HCV) is a major cause of liver diseases including liver cirrhosis and hepatocellular carcinoma. Approximately 3% of the world population is infected with HCV. Thus, HCV infection is considered a public healthy challenge. It is worth mentioning, that the HCV prevalence is dependent on the countries with infection rates around 20% in high endemic countries. The review summarizes recent data on HCV molecular biology, the physiopathology of infection(immune-mediated liver damage, liver fibrosis and lipid metabolism), virus diagnostic and treatment. In addition, currently available in vitro, ex vivo and animal models to study the virus life cycle, virus pathogenesis and therapy are described. Understanding of both host and viral factors may in the future lead to creation of new approaches in generation of an efficient therapeutic vaccine.
文摘Catalytic fast pyrolysis （CFP） is deemed as the most promising way to convert biomass to transportation fuels or value added chemicals. Most works in literature so far have focused on the in situ CFP where the catalysts are packed or co-fed with the feedstock in the pyrolysis reactor. However, the ex situ CFP with catalysts separated from the pyrolyzer has attracted more and more attentions due to its unique advantages of individually optimizing the pyrolysis conditions and catalyst performances. This review compares the differences between the in situ and ex situ CFP operation, and summarizes the development and progress of ex situ CFP applications, including the rationale and performances of different catalysts, and the choices of suitable ex situ reactor systems. Due to the complex composition of bio-oil, no single approach was believed to be able to solve the problems completely among all those existing technologies. With the increased understanding of catalyst performances and reaction process, the recent trend toward an integration of biomass or bio-oil fractionation with subsequent thermo/biochemical conversion routes is also discussed.
基金Supported by the Scientific and Technologic Project of Jiangsu Administration of TCM(No.LZ13243)
文摘OBJECTIVE: To evaluate the clinical effect of traditional Chinese spinal orthopedic manipulation(TCSOM) in treating chondromalacia patellae(CP).METHODS: Sixty cases of CP patients were randomly assigned to a TCSOM group and a Celecoxib group according to the random number table method. All patients in the TCSOM group were treated with a maximum of 10 spinal manipulations and rehabilitation training of quadriceps femoris. The symptoms before and after treatment were assessed with visual analog scale(VAS) and Kujala functional knee scoring system(KFKSS). A symptom improvement rate(SIR) was implemented in order to evaluate the effects of the treatments.RESULTS: The symptoms of 16 patients in the TC-SOM group quickly resolved after the first spinal manipulation and 8 cases were significantly improved. The VAS scores in the TCSOM group after 4weeks of treatment were significantly lower than those in the Celecoxib group. The KFKSS scores in the TCSOM group after 4 weeks of treatment were significantly higher than those in the Celecoxib group. Side effects of the treatment were not reported. Symptom improvement rate based on the VAS in the TCSOM group indicated more significant improvements than the Celecoxib group.CONCLUSION: TCSOM has greater efficacy than Celecoxib capsules for relief of the symptoms of CP.
基金Supported by the National Natural Science Foundation of China （30370153）, and the Key Project of the Knowledge Innovation of the Chinese Academy of Sciences （KSCX2-SW-104）.
文摘Spontaneous hybridization in ex situ facilities can undermine the genetic integrity of ex situ collections and potentially contaminate open-pollinated seeds or seedlings destined for the reintroduction of endangered plant species into the wild. In the present study, the potential risk of hybridization between two endangered Chinese endemic species, namely Sinojackia xylocarpa Hu and S. rehderiana Hu, which are naturally allopatric species but were conserved ex situ in Wuhan Botanical Garden （WBG）, Wuhan, China, were investigated over three consecutive years from 2003 to 2005. The entire overlapping flowering period of the two species was 14-20 d and the two species shared the same pollinator insects during the entire flowering season in WBG. The floral isolation between the two species was not an issue in the ex sltu collection at WBG. The results suggest an opportunity for pollen transfer between species and a potential risk of genetic Introgression and loss of genetic identity of open-pollinated seeds produced in the ex sltu Collection of these two endangered species. An artificial reciprocal cross between S xylocarpa and S. rehderlana confirmed that the two congener species could readily set seeds, indicating no post-pollination barriers to hybridization and the importance of spatial isolation as a barrier to inter-specific crossing. Therefore, to manage these crossable species with overlapping flowering times and shared pollination vectors in ex situ facilities, spatial isolation should be carefully considered to minimize the possibility of spontaneous hybridization.
