Two new indole alkaloids. wuchuyuamide I and II were isolated from the fruits of Evodia rutaecarpa (Juss.) Benth and their structures were elucidated on the basis of spectral data.
目的:研究吴茱萸碱对高脂血症小鼠脂质代谢及低密度脂蛋白-受体(LDL-R) m RNA表达的影响。方法:昆明种小鼠(n=80)随机分为正常组(n=20)和模型组(n=60)。模型组高脂饲料饲养3周后检测血脂,造模成功后随机分每5组,每组10只,模型组(等量生...目的:研究吴茱萸碱对高脂血症小鼠脂质代谢及低密度脂蛋白-受体(LDL-R) m RNA表达的影响。方法:昆明种小鼠(n=80)随机分为正常组(n=20)和模型组(n=60)。模型组高脂饲料饲养3周后检测血脂,造模成功后随机分每5组,每组10只,模型组(等量生理盐水)、阳性药组(辛伐他汀5 mg·kg-1),给药组(吴茱萸碱5. 25,10. 5,21 mg·kg-1),给药3周。苏木素-伊红(HE)染色切片观察小鼠肝细胞结构及主动脉弓粥样改变程度,用酶联免疫试剂盒测试各小鼠血清中总胆固醇(TC),甘油三酯(TG),高密度脂蛋白胆固醇(HDL-C),低密度脂蛋白胆固醇(LDL-C)与血清总脂联素(ADPN)的含量。采用逆转录-聚合酶链式反应(RT-PCR)的方法,检测各组小鼠肝脏LDL-R m RNA的表达。结果:HE染色切片可见模型组小鼠肝细胞肿胀并伴有脂肪变性,吴茱萸碱高、中剂量及辛伐他汀组可改善肝脏脂肪变性。模型组小鼠主动脉弓管壁有损伤、有明显内膜增厚现象、炎细胞浸润;吴茱萸碱低剂量组内膜增厚明显,高剂量组小鼠主动脉血管壁结构较清晰,少见内皮损伤。与正常组比较,模型组小鼠血清中TC,TG,LDL-C升高,HDL-C水平降低(P <0. 01);与模型组比较,吴茱萸碱中、高剂量组小鼠血清TC,TG水平降低,吴茱萸碱低、中、高剂量组LDL-C降低,HDL-C水平升高(P <0. 05,P <0. 01)。与正常组比较,模型组脂联素水平降低,与模型组比较,吴茱萸碱中、高剂量组的血清脂联素水平明显升高(P <0. 01)。与正常组比较,模型组小鼠肝脏LDL-R m RNA的表达明显降低(P <0. 01);与模型组比较,吴茱萸碱中、高剂量组LDL-R m RNA表达明显增强(P <0. 01)。结论:吴茱萸碱能改善高脂血症小鼠肝脏病变、主动脉粥样硬化趋势,可能与通过调节脂联素水平,降低小鼠血脂含量及上调小鼠肝脏LDL-R m RNA的表达有关。展开更多
Objective To control the quality of Evodia rutaecarpa better.Methods An HPLC-DAD-MS/MS method was established for the rapid and efficient identification of bioactive constituents and for simultaneous quantitative anal...Objective To control the quality of Evodia rutaecarpa better.Methods An HPLC-DAD-MS/MS method was established for the rapid and efficient identification of bioactive constituents and for simultaneous quantitative analysis of four bioactive ingredients including evodiamine,rutaecarpine,dehydroevodiamine,and evodin in E.rutaecarpa,which was applied to evaluating eight samples of E.rutaecarpa and its varieties from different areas.Results Thirteen potentially bioactive constituents including one flavonoid glycoside,one limonin,four indoloquinazoline alkaloids,and seven quinolone alkaloids were identified in all samples and the contents of dehydroevodiamine,evodine,evodiamine,and rutaecarpine varied widely from 0.10% to 0.51%,0.49% to 3.12%,0.07% to 1.56%,and 0.10% to 0.69%,respectively.Conclusion This method is found to be convenient,fast,accurate,and it is facilitated to improve the quality control standard of E.rutaecarpa and related products.展开更多
文摘Two new indole alkaloids. wuchuyuamide I and II were isolated from the fruits of Evodia rutaecarpa (Juss.) Benth and their structures were elucidated on the basis of spectral data.
文摘Objective To control the quality of Evodia rutaecarpa better.Methods An HPLC-DAD-MS/MS method was established for the rapid and efficient identification of bioactive constituents and for simultaneous quantitative analysis of four bioactive ingredients including evodiamine,rutaecarpine,dehydroevodiamine,and evodin in E.rutaecarpa,which was applied to evaluating eight samples of E.rutaecarpa and its varieties from different areas.Results Thirteen potentially bioactive constituents including one flavonoid glycoside,one limonin,four indoloquinazoline alkaloids,and seven quinolone alkaloids were identified in all samples and the contents of dehydroevodiamine,evodine,evodiamine,and rutaecarpine varied widely from 0.10% to 0.51%,0.49% to 3.12%,0.07% to 1.56%,and 0.10% to 0.69%,respectively.Conclusion This method is found to be convenient,fast,accurate,and it is facilitated to improve the quality control standard of E.rutaecarpa and related products.
