AIM: To evaluate the functional aspect of esophageal motility in healthy subjects and in patients who were referred for esophageal function testing using multichannel intraluminal impedance-esophageal manometry (MII-E...AIM: To evaluate the functional aspect of esophageal motility in healthy subjects and in patients who were referred for esophageal function testing using multichannel intraluminal impedance-esophageal manometry (MII-EM), and to assess the clinical utility of MII-EM. METHODS: From September 2003 to January 2004, we performed the MII-EM on healthy volunteers and all the patients who were referred for esophageal function testing. Each patient received 10 liquid and 10 viscous swallows. We analyzed the results, the impedance and the manometric findings. Some of the subjects had additional ambulatory 24-h pH study performed to diagnose gastroesophageal reflux disease (GERD). RESULTS: Among 89 studied subjects, the MII-EM findings showed normal esophageal motility in 50 (56.17%), ineffective esophageal motility (IEM) in 17 (19.10%), nutcracker esophagus in 7 (7.86%), achalasia in 4 (4.49%), and scleroderma esophagus in 11 (12.35%) cases. The completeness and the speed of bolus transit were in the order of nutcracker esophagus, normal manometry and IEM. Some of the swallows showing normal manometry and IEM had incomplete transit. In the achalasia and scleroderma esophagus, almost all the swallows had incomplete transit. The body amplitudes were higher for the swallows with complete transit than for the swallows with incomplete transit. There was not a significant difference in the manometric and impedance findings between the subjects with and without GERD. CONCLUSION: MII-EM is a useful tool in assessing theesophageal function in the patients having esophageal motility abnormality. The primary factors influencing the bolus transit are the amplitude of the esophageal body and normal peristalsis.展开更多
Context: In spite of the development of cross-sectional medical imaging and endoscopy means, the prescription of specialized radiographic examinations is still relevant in the pediatric environment in our country. Obj...Context: In spite of the development of cross-sectional medical imaging and endoscopy means, the prescription of specialized radiographic examinations is still relevant in the pediatric environment in our country. Objective: Show the contribution of specialized radiographic examinations performed in pediatric settings. Methodology: This was a retrospective descriptive study implemented over a five year-period, from June 10<sup>th</sup>, 2014 to June 10<sup>th</sup>, 2019 within the radiology department of Charles De Gaulle Pediatric Teaching Hospital in Ouagadougou. This study included the results of 192 specialized radiographic examinations collected within the concerned department. Results: The average age of the patients was 4.21 years with extremes of 8 days and 15 years. Infants (29 days to 24 months) were the predominant age group with a rate of 53.13% (n = 102 patients). Boys represented 76.51% of the sample with a sex ratio estimated at 3.25. Specialized radiographic examinations represented 0.46% of the overall imaging examinations and 0.72% of radiographic examinations performed during the study period. 67.19% of pathological examinations were reported. Colonic enema and retrograde urethrocystography (RUC) were the most performed examinations respectively accounting for 33.34% and 27.6%. The radiographic findings were consistent with the diagnostic hypotheses in 41.6% of cases. Conclusion: Specialized radiographic examinations keep their place in the exploration of several pathologies in pediatric settings.展开更多
目的研究Gli表达下调对食管腺癌OE33细胞生长转移的影响,并探讨其相关的分子机制。方法将Gli1、Gli2 si RNA和空白对照si RNA转染OE33细胞48 h,实时荧光定量聚合酶链反应检测OE33细胞Gli1和Gli2 m RNA表达水平;采用Western blot检测OE3...目的研究Gli表达下调对食管腺癌OE33细胞生长转移的影响,并探讨其相关的分子机制。方法将Gli1、Gli2 si RNA和空白对照si RNA转染OE33细胞48 h,实时荧光定量聚合酶链反应检测OE33细胞Gli1和Gli2 m RNA表达水平;采用Western blot检测OE33细胞中Gli1、Gli2、Cyclin D1、p27、E-cadherin及N-cadherin蛋白的表达水平;流式细胞术检测OE33细胞增殖和细胞周期分布;Transwell实验检测细胞侵袭能力。结果采用Gli1和Gli2 si RNA抑制Gli1和Gli2 m RNA及蛋白表达后,可下调Cyclin D1和N-cadherin蛋白表达,增加E-cadherin和p27蛋白表达,同时抑制细胞增殖,将细胞周期阻滞在G_0/G_1期并降低细胞的侵袭能力,与对照组比较,差异有统计学意义(P<0.05)。结论 Gli在食管腺癌的生长转移中具有重要作用,敲掉Gli1和Gli2基因表达可抑制食管腺癌细胞周期分布和上皮-间质转化能力,这可能与Cyclin D1、p27、E-cadherin及N-cadherin蛋白变化相关。展开更多
文摘AIM: To evaluate the functional aspect of esophageal motility in healthy subjects and in patients who were referred for esophageal function testing using multichannel intraluminal impedance-esophageal manometry (MII-EM), and to assess the clinical utility of MII-EM. METHODS: From September 2003 to January 2004, we performed the MII-EM on healthy volunteers and all the patients who were referred for esophageal function testing. Each patient received 10 liquid and 10 viscous swallows. We analyzed the results, the impedance and the manometric findings. Some of the subjects had additional ambulatory 24-h pH study performed to diagnose gastroesophageal reflux disease (GERD). RESULTS: Among 89 studied subjects, the MII-EM findings showed normal esophageal motility in 50 (56.17%), ineffective esophageal motility (IEM) in 17 (19.10%), nutcracker esophagus in 7 (7.86%), achalasia in 4 (4.49%), and scleroderma esophagus in 11 (12.35%) cases. The completeness and the speed of bolus transit were in the order of nutcracker esophagus, normal manometry and IEM. Some of the swallows showing normal manometry and IEM had incomplete transit. In the achalasia and scleroderma esophagus, almost all the swallows had incomplete transit. The body amplitudes were higher for the swallows with complete transit than for the swallows with incomplete transit. There was not a significant difference in the manometric and impedance findings between the subjects with and without GERD. CONCLUSION: MII-EM is a useful tool in assessing theesophageal function in the patients having esophageal motility abnormality. The primary factors influencing the bolus transit are the amplitude of the esophageal body and normal peristalsis.
文摘Context: In spite of the development of cross-sectional medical imaging and endoscopy means, the prescription of specialized radiographic examinations is still relevant in the pediatric environment in our country. Objective: Show the contribution of specialized radiographic examinations performed in pediatric settings. Methodology: This was a retrospective descriptive study implemented over a five year-period, from June 10<sup>th</sup>, 2014 to June 10<sup>th</sup>, 2019 within the radiology department of Charles De Gaulle Pediatric Teaching Hospital in Ouagadougou. This study included the results of 192 specialized radiographic examinations collected within the concerned department. Results: The average age of the patients was 4.21 years with extremes of 8 days and 15 years. Infants (29 days to 24 months) were the predominant age group with a rate of 53.13% (n = 102 patients). Boys represented 76.51% of the sample with a sex ratio estimated at 3.25. Specialized radiographic examinations represented 0.46% of the overall imaging examinations and 0.72% of radiographic examinations performed during the study period. 67.19% of pathological examinations were reported. Colonic enema and retrograde urethrocystography (RUC) were the most performed examinations respectively accounting for 33.34% and 27.6%. The radiographic findings were consistent with the diagnostic hypotheses in 41.6% of cases. Conclusion: Specialized radiographic examinations keep their place in the exploration of several pathologies in pediatric settings.
文摘目的研究Gli表达下调对食管腺癌OE33细胞生长转移的影响,并探讨其相关的分子机制。方法将Gli1、Gli2 si RNA和空白对照si RNA转染OE33细胞48 h,实时荧光定量聚合酶链反应检测OE33细胞Gli1和Gli2 m RNA表达水平;采用Western blot检测OE33细胞中Gli1、Gli2、Cyclin D1、p27、E-cadherin及N-cadherin蛋白的表达水平;流式细胞术检测OE33细胞增殖和细胞周期分布;Transwell实验检测细胞侵袭能力。结果采用Gli1和Gli2 si RNA抑制Gli1和Gli2 m RNA及蛋白表达后,可下调Cyclin D1和N-cadherin蛋白表达,增加E-cadherin和p27蛋白表达,同时抑制细胞增殖,将细胞周期阻滞在G_0/G_1期并降低细胞的侵袭能力,与对照组比较,差异有统计学意义(P<0.05)。结论 Gli在食管腺癌的生长转移中具有重要作用,敲掉Gli1和Gli2基因表达可抑制食管腺癌细胞周期分布和上皮-间质转化能力,这可能与Cyclin D1、p27、E-cadherin及N-cadherin蛋白变化相关。
