Abstract: Objective To establish a new procedure for isolating scutellarin from Erigeron multiradiatus. Methods Aproposed method was developed by combining macroporous resins with ODS column. Firstly, E. multiradiatus...Abstract: Objective To establish a new procedure for isolating scutellarin from Erigeron multiradiatus. Methods Aproposed method was developed by combining macroporous resins with ODS column. Firstly, E. multiradiatus wasextracted by ultrasound with 80% methanol. Preliminary separation was performed on macroporous resin column.The performance and adsorption characteristics of three macroporous resins, D140, D141, and D605, were comparedand the enrichment procedure was optimized. Further purification was carried out by medium pressure liquidchromatography (MPLC) with ODS column. Results It was demonstrated that D141 had better extractive effects onscutellarin. The MPLC conditions were optimized as follows: 15% ethanol aqueous as mobile phase with flow rate at2.5 mL/min. The yield and purity of the isolated scutellarin were 1.20 mg/g and 96.5%, respectively. ConclusionThe overall procedure is efficient and low-cost, which is considered suitable for the separation and purification ofscutellarin from E. multiradiatus. The results provide the scientific basis for developing and using scutellarin in clinic.展开更多
Objective To explore the effects of a potential anti-oxidative plant,Erigeron multiradiatus(Asteraceae) ,plant materials from naturally distributed high-altitude populations at different stages of life cycle were coll...Objective To explore the effects of a potential anti-oxidative plant,Erigeron multiradiatus(Asteraceae) ,plant materials from naturally distributed high-altitude populations at different stages of life cycle were collected.Methods Fifteen extracts obtained from the Ganzi region(Sichuan,China) were studied to assess their radical-scavenging ability on 1,1-diphenyl-2-picrylhydrazyl radicals and reducing power ability.Moreover,considering that anti-oxidants and free radical scavengers can also exert protective effect on endothelial cells from oxidative injury,these extracts were also evaluated for their anti-oxidative activity against cellular injury in the cultured human endothelial cell line(ECV304) induced by hydrogen peroxide(H2O2) .Results All the extracts had radical-scavenging and/or reducing power ability,and the most active extract was found during flowering whereas the lowest appeared during vegetative growth period.The accumulation of anti-oxidative compounds was found to be affected by the altitude of growth environment.Total flavonoid content assay was also performed to support this outcome.Furthermore,these extracts also exhibited different effects on attenuating H2O2-induced cytotoxicity and inhibiting lipid peroxidation and LDH leakage from endothelial cells.Conclusion E.multiradiatus may be an important natural anti-oxidant and this property may contribute to verifying the utilization of this plant in Tibet folk medicine.展开更多
考察了13种不同极性的大孔吸附树脂对多舌飞蓬中总咖啡酸酯的富集纯化能力,发现极性树脂NKA-2的静态吸附率为92.51%、解吸率为98.51%,明显优于其他树脂。进一步以NKA-2极性树脂对多舌飞蓬总咖啡酸酯进行静态吸附与动态吸附实验,结果表明...考察了13种不同极性的大孔吸附树脂对多舌飞蓬中总咖啡酸酯的富集纯化能力,发现极性树脂NKA-2的静态吸附率为92.51%、解吸率为98.51%,明显优于其他树脂。进一步以NKA-2极性树脂对多舌飞蓬总咖啡酸酯进行静态吸附与动态吸附实验,结果表明,最佳上样质量浓度为300 g/L,最佳上样流速为2 BV/h(BV/h即每小时内,通过床层液体体积的倍数),树脂吸附容量为6 m L/g,即每克NKA-2树脂可以处理1.8 g生药。解吸实验结果表明,用体积分数为60%的乙醇水溶液洗脱,洗脱剂用量为30 m L/g树脂。用上述条件对多舌飞蓬总咖啡酸酯富集纯化,使总咖啡酸酯的纯度由18.09%提高到58.06%,回收率高达94.78%。说明NKA-2型大孔吸附树脂综合性能良好,可用于多舌飞蓬中总咖啡酸酯成分的富集和纯化。展开更多
Erigeron multiradiatus(Lindl.)Benth.,has been used in Tibet folk medicine to treat various inflammatory diseases.The aim of this study was to investigate anti-myocardial ischemia and reperfusion(I/R)injury effect of c...Erigeron multiradiatus(Lindl.)Benth.,has been used in Tibet folk medicine to treat various inflammatory diseases.The aim of this study was to investigate anti-myocardial ischemia and reperfusion(I/R)injury effect of caffeoylquinic acids derivatives of E.multiradiatus(AE)in vivo and to explain underling mechanism.AE was prepared using the whole plant of E.multiradiatus and contents of 6 caffeoylquinic acid determined through HPLC analysis.Myocardial I/R were induced by left anterior descending coronary artery occlusion for 30 min followed by 24 h of reperfusion in rats.AE administration(10,20 and 40 mg·kg-1)inhibited I/R-induced injury as indicated by decreasing myocardial infarct size,reducing of CK and LDH activities and preventing ST-segment depression in dose-dependent manner.AE decreased cardiac tissue levels of pro-inflammatory factors TNF-αand IL-6 and attenuated leukocytes infiltration.AE was further demonstrated to significantly inhibit I-κB degradation,nuclear translocation of p-65 and phosphorylation of JNK.Our results suggested that cardioprotective effect of AE could be due to suppressing myocardial inflammatory response and blocking NF-κB and JNK activation pathway.Thus,caffeoylquinic acids might be the active compounds in E.multiradiatus on myocardial ischemia and be a potential natural drug for treating myocardial I/R injury.展开更多
基金Fundamental Research Funds for Central Universities,Southwest University for Nationalities(09NZYZJ01)National Key Technology R&D Program in the 12th Five Year Plan of China(2012BAI27B07)
文摘Abstract: Objective To establish a new procedure for isolating scutellarin from Erigeron multiradiatus. Methods Aproposed method was developed by combining macroporous resins with ODS column. Firstly, E. multiradiatus wasextracted by ultrasound with 80% methanol. Preliminary separation was performed on macroporous resin column.The performance and adsorption characteristics of three macroporous resins, D140, D141, and D605, were comparedand the enrichment procedure was optimized. Further purification was carried out by medium pressure liquidchromatography (MPLC) with ODS column. Results It was demonstrated that D141 had better extractive effects onscutellarin. The MPLC conditions were optimized as follows: 15% ethanol aqueous as mobile phase with flow rate at2.5 mL/min. The yield and purity of the isolated scutellarin were 1.20 mg/g and 96.5%, respectively. ConclusionThe overall procedure is efficient and low-cost, which is considered suitable for the separation and purification ofscutellarin from E. multiradiatus. The results provide the scientific basis for developing and using scutellarin in clinic.
基金Applied Fundamental Research Scheme of the Bureau of Science and Technology of Sichuan Province, China (2009JY0017)the Fundamental Research Funds for the Central Universities, Southwest University for Nationalities (09NZYZJ01)
文摘Objective To explore the effects of a potential anti-oxidative plant,Erigeron multiradiatus(Asteraceae) ,plant materials from naturally distributed high-altitude populations at different stages of life cycle were collected.Methods Fifteen extracts obtained from the Ganzi region(Sichuan,China) were studied to assess their radical-scavenging ability on 1,1-diphenyl-2-picrylhydrazyl radicals and reducing power ability.Moreover,considering that anti-oxidants and free radical scavengers can also exert protective effect on endothelial cells from oxidative injury,these extracts were also evaluated for their anti-oxidative activity against cellular injury in the cultured human endothelial cell line(ECV304) induced by hydrogen peroxide(H2O2) .Results All the extracts had radical-scavenging and/or reducing power ability,and the most active extract was found during flowering whereas the lowest appeared during vegetative growth period.The accumulation of anti-oxidative compounds was found to be affected by the altitude of growth environment.Total flavonoid content assay was also performed to support this outcome.Furthermore,these extracts also exhibited different effects on attenuating H2O2-induced cytotoxicity and inhibiting lipid peroxidation and LDH leakage from endothelial cells.Conclusion E.multiradiatus may be an important natural anti-oxidant and this property may contribute to verifying the utilization of this plant in Tibet folk medicine.
文摘考察了13种不同极性的大孔吸附树脂对多舌飞蓬中总咖啡酸酯的富集纯化能力,发现极性树脂NKA-2的静态吸附率为92.51%、解吸率为98.51%,明显优于其他树脂。进一步以NKA-2极性树脂对多舌飞蓬总咖啡酸酯进行静态吸附与动态吸附实验,结果表明,最佳上样质量浓度为300 g/L,最佳上样流速为2 BV/h(BV/h即每小时内,通过床层液体体积的倍数),树脂吸附容量为6 m L/g,即每克NKA-2树脂可以处理1.8 g生药。解吸实验结果表明,用体积分数为60%的乙醇水溶液洗脱,洗脱剂用量为30 m L/g树脂。用上述条件对多舌飞蓬总咖啡酸酯富集纯化,使总咖啡酸酯的纯度由18.09%提高到58.06%,回收率高达94.78%。说明NKA-2型大孔吸附树脂综合性能良好,可用于多舌飞蓬中总咖啡酸酯成分的富集和纯化。
基金The project supported by the Macao Science and Technology Development Fund(052/2013/A2)
文摘Erigeron multiradiatus(Lindl.)Benth.,has been used in Tibet folk medicine to treat various inflammatory diseases.The aim of this study was to investigate anti-myocardial ischemia and reperfusion(I/R)injury effect of caffeoylquinic acids derivatives of E.multiradiatus(AE)in vivo and to explain underling mechanism.AE was prepared using the whole plant of E.multiradiatus and contents of 6 caffeoylquinic acid determined through HPLC analysis.Myocardial I/R were induced by left anterior descending coronary artery occlusion for 30 min followed by 24 h of reperfusion in rats.AE administration(10,20 and 40 mg·kg-1)inhibited I/R-induced injury as indicated by decreasing myocardial infarct size,reducing of CK and LDH activities and preventing ST-segment depression in dose-dependent manner.AE decreased cardiac tissue levels of pro-inflammatory factors TNF-αand IL-6 and attenuated leukocytes infiltration.AE was further demonstrated to significantly inhibit I-κB degradation,nuclear translocation of p-65 and phosphorylation of JNK.Our results suggested that cardioprotective effect of AE could be due to suppressing myocardial inflammatory response and blocking NF-κB and JNK activation pathway.Thus,caffeoylquinic acids might be the active compounds in E.multiradiatus on myocardial ischemia and be a potential natural drug for treating myocardial I/R injury.
