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四神丸对腹泻型肠易激综合征大鼠肠道菌群影响的实验研究 被引量:59
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作者 刘佳星 王彦礼 +6 位作者 李彧 邹迪新 王敦方 马旭冉 宋红新 杨伟鹏 王海南 《药学学报》 CAS CSCD 北大核心 2019年第4期670-677,共8页
研究四神丸对腹泻型肠易激综合征(diarrhea-predominant irritable bowel syndrome, IBS-D)大鼠肠道菌群的影响,探讨其改善IBS-D与肠道菌群的关系。选取SPF级雄性SD大鼠45只,随机分为正常组、模型组、四神丸组、二神丸组和五味子散组。... 研究四神丸对腹泻型肠易激综合征(diarrhea-predominant irritable bowel syndrome, IBS-D)大鼠肠道菌群的影响,探讨其改善IBS-D与肠道菌群的关系。选取SPF级雄性SD大鼠45只,随机分为正常组、模型组、四神丸组、二神丸组和五味子散组。以复合因素制备脾肾阳虚型IBS-D大鼠模型后给药14天,采集大鼠粪便,提取粪便样本总DNA,根据细菌16S r RNA V3~V4区设计引物进行扩增,利用Illumina Miseq平台进行高通量测序。动物实验操作过程依照中国中医科学院动物实验伦理委员会的要求执行。研究发现:四神丸能有效减小IBS-D大鼠腹泻指数(P<0.05)及降低肠道高敏感性(P<0.05)。主成分分析(PCA)、主坐标分析(PCoA)和基于β多样性距离的非度量多维尺度分析(NMDS)结果显示5组大鼠肠道菌群组成存在明显差异。模型组的菌群丰度、均匀度和多样性均最低。与正常组相比,模型组变形菌门、放线菌门、韦荣球菌属和支原体属显著增加(P<0.05),普雷沃氏菌属显著减少(P<0.05)。与模型组相比,四神丸组变形菌门和支原体属显著减少(P<0.05),梭菌属、Turicibacter和Romboutsia显著增加(P<0.05)。本研究表明四神丸可能是部分通过调节肠道菌群的结构而发挥治疗IBS-D的作用,且二神丸和五味子散之间有协同作用。 展开更多
关键词 四神丸 二神丸 五味子散 腹泻型肠易激综合征 肠道菌群 高通量测序
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Modified Tong Xie Yao Fang relieves solitary rectal ulcer syndrome:A case report 被引量:2
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作者 Li-Li Zhang Wan-Shan Hao +2 位作者 Meng Xu Chang Li Yuan-Yuan Shi 《World Journal of Clinical Cases》 SCIE 2019年第15期2058-2064,共7页
BACKGROUND Solitary rectal ulcer syndrome(SRUS)is a rare rectal disorder characterized by bloody mucus in the stool,difficulty in defecation,pain,and anal swelling.To date,the etiology of this syndrome remains not wel... BACKGROUND Solitary rectal ulcer syndrome(SRUS)is a rare rectal disorder characterized by bloody mucus in the stool,difficulty in defecation,pain,and anal swelling.To date,the etiology of this syndrome remains not well understood and the diagnosis is frequently confused with other disorders,making treatment a clinical challenge.CASE SUMMARY A 50-year-old woman presented to our hospital with a 40-d history of bloody mucus in the stool and anal swelling.SRUS was suspected.Rectoscopy revealed a large,severe ulcerous lesion.Histologically,the lesion was characterized as chronic ulcer without clear tumor cells,and the final diagnosis of SRUS was made.The patient was treated with Chinese medicine therapy,with administration of Tong Xie Yao Fang.After 3 wk of treatment,the symptoms improved significantly.At 2-mo follow-up,rectoscopy in a local hospital showed healed ulcer scars without obvious protrusion 3 cm from the anal verge.CONCLUSION Chinese medicine therapy represents a potential treatment of SRUS with predominant rectal bleeding,mucinous discharge,and anal swelling pain. 展开更多
关键词 Solitary RECTAL ULCer syndrome CHINESE formulas Tong Xie YAO Fang er shen wan Ding Zhi Xiao wan CHINESE medicine therapy Case report
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