INTRODUCTION Chronic obstructive pulmonary disease （COPD） is one of the leading causes of death in the developed world and associated with a high individual and socioeconomic burden. It is characterized by persisten...INTRODUCTION Chronic obstructive pulmonary disease （COPD） is one of the leading causes of death in the developed world and associated with a high individual and socioeconomic burden. It is characterized by persistent airflow limitation that is usually progressive and associated with an enhanced chronic inflammatory response in the airways and the lung to noxious panicles or gases. Peri-bronchiolar fibrosis was occurred in small airways in the early state of COPD, and then followed by structure changes, and finally became persistent airflow limitation？21 Recent researches have shown that epithelial-mesenchymal transition （EMT） is one of the leading causes of fibrosis in various diseases.展开更多
Objective： Metadherin （MTDH） could regulate epithelial-mesenchymal transition （EMT）, and is involved in tumor metastasis.This study was designed to observe the effect of MTDH-short hairpin RNA （shRNA） on EMT, a...Objective： Metadherin （MTDH） could regulate epithelial-mesenchymal transition （EMT）, and is involved in tumor metastasis.This study was designed to observe the effect of MTDH-short hairpin RNA （shRNA） on EMT, and the role of MTDH in breast tumor metastasis. Methods： RNA interference plasmid that can express shRNA targeting MTDH or shRNA-nega- tive plasmid that does not match any known human coding mRNA was designed, constructed and named MTDH-shRNA and MTDH-shRNA-neg, which were transiently transfected into MDA-MB-231 cells using LipofectamineTM2000. After 48 h, the levels of MTDH, E-cadherin and a-SMA expression were determined by reverse transcription-polymerase chain reactin （RT- PCR）, Western blot. The invasion and immigration potential was examined by Transwell chamber invasion or migration assay. Results： Compared with MDA-MB-231, the MTDH mRNA level was down-regulated by 41.2%, the MTDH protein level was down-regulated by 40.3%. The invasion and immigration potential of MDA-MB-231 cells was decreased after transfection of MTDH-shRNA. Compared with MDA-MB-231 or MTDH-shRNA-neg, the mRNA and protein level of a-SMA was reduced and E-candherin were increased in MTDH-shRNA, with statisticat significance. Conclusion： Downregulation of MTDH increase E-candherin expression and reduced a-SMA expression, which inhibit EMT in MDA-MB-231 cells. This knockdown significantly suppresse migration and invasion in MDA-MB-231 cells.展开更多
文摘INTRODUCTION Chronic obstructive pulmonary disease （COPD） is one of the leading causes of death in the developed world and associated with a high individual and socioeconomic burden. It is characterized by persistent airflow limitation that is usually progressive and associated with an enhanced chronic inflammatory response in the airways and the lung to noxious panicles or gases. Peri-bronchiolar fibrosis was occurred in small airways in the early state of COPD, and then followed by structure changes, and finally became persistent airflow limitation？21 Recent researches have shown that epithelial-mesenchymal transition （EMT） is one of the leading causes of fibrosis in various diseases.
基金Supported by grants from the Sub-topics of Major Discovery Platform in the 12th Five-Year Program of China (No. 2012ZX09303016-002)the Liaoning Province Science & Technology Department Funds (No. 201002060)
文摘Objective： Metadherin （MTDH） could regulate epithelial-mesenchymal transition （EMT）, and is involved in tumor metastasis.This study was designed to observe the effect of MTDH-short hairpin RNA （shRNA） on EMT, and the role of MTDH in breast tumor metastasis. Methods： RNA interference plasmid that can express shRNA targeting MTDH or shRNA-nega- tive plasmid that does not match any known human coding mRNA was designed, constructed and named MTDH-shRNA and MTDH-shRNA-neg, which were transiently transfected into MDA-MB-231 cells using LipofectamineTM2000. After 48 h, the levels of MTDH, E-cadherin and a-SMA expression were determined by reverse transcription-polymerase chain reactin （RT- PCR）, Western blot. The invasion and immigration potential was examined by Transwell chamber invasion or migration assay. Results： Compared with MDA-MB-231, the MTDH mRNA level was down-regulated by 41.2%, the MTDH protein level was down-regulated by 40.3%. The invasion and immigration potential of MDA-MB-231 cells was decreased after transfection of MTDH-shRNA. Compared with MDA-MB-231 or MTDH-shRNA-neg, the mRNA and protein level of a-SMA was reduced and E-candherin were increased in MTDH-shRNA, with statisticat significance. Conclusion： Downregulation of MTDH increase E-candherin expression and reduced a-SMA expression, which inhibit EMT in MDA-MB-231 cells. This knockdown significantly suppresse migration and invasion in MDA-MB-231 cells.
