The repair of peripheral nerve injury after complete amputation is difficult,and even with anastomosis,the rapid recovery of nerve function remains challenging.Curcumin,extracted from plants of the genus Curcuma,has b...The repair of peripheral nerve injury after complete amputation is difficult,and even with anastomosis,the rapid recovery of nerve function remains challenging.Curcumin,extracted from plants of the genus Curcuma,has been shown to have anti-oxidant and anti-inflammatory properties and to improve sciatic nerve crush injury in rats.Here,we determined whether curcumin had neuroprotective effects following complete peripheral nerve amputation injury.BALB/c mice underwent complete sciatic nerve amputation,followed by an immediate epineurium anastomosis.Mice were intragastrically administered curcumin at doses of 40（high）,20（moderate）,and 10 mg/kg/d（low） for 1 week.We found that myelin in the mice of the high- and moderate-dose curcumin groups appeared with regular shape,uniform thickness,clear boundary,and little hyperplasia surrounding the myelin.High and moderate doses of curcumin markedly improved both action potential amplitude of the sciatic nerves and the conduction velocity of the corresponding motor neurons,and upregulated m RNA and protein expression of S100,a marker for Schwann cell proliferation,in L4–6 spinal cord segments.These results suggest that curcumin is effective in promoting the repair of complete sciatic nerve amputation injury and that the underlying mechanism may be associated with upregulation of S100 expression.展开更多
A number of studies have shown how to eliminate the misorientated docking of the peripheral nerve bundle in the traditional epineurium or perineurium anastomosis, thus avoiding neuroma formation and axonal outgrowth f...A number of studies have shown how to eliminate the misorientated docking of the peripheral nerve bundle in the traditional epineurium or perineurium anastomosis, thus avoiding neuroma formation and axonal outgrowth from the coaptation sites, and seriously hindering neural function recovery. Based on the ＂peripheral nerve seJective regeneration theory＂, this experiment was designed to investigate the feasibility and benefits of a new small gap anastomosis repairing peripheral nerve rupture, by scissoring and sleeve jointing an autologous epineufium, in the proximal stump of the nerve, a 1 mm-long epineurium was annularly separated and removed, while a 3 mm-long epineurium was longitudinally incised in the distal stump after the epineurium was dissociated from proximal to distal. The epineuria of the two stumps and the longitudinal incision were sutured, leaving a 2 mm gap between the two nerve stumps. Results show that the experimental rats quickly recovered autonomic activities, and there were minimal adhesions at the outer surface of the epineudal tube to the surrounding tissue. The morphologJc changes to the sciatic nerve showed that connective tissue hyperplasia of the small gaps was significantly reduced, and nerve fibers were arranged orderly. No such changes were observed in the neurorrhaphy in situ group. Thus, the experiment confirmed that the new small gap anastomosis to repair peripheral nerve rupture by scissoring and sleeve jointing autologous epineurium is feasible, and that it is superior to epineurium neurorrhaphy in situ.展开更多
目的了解喉返神经解剖结构的特点对于今后神经修复和器官移植的意义。方法采取6只成年狗的右侧、同长度的颈部喉返神经和坐骨神经末端分支处至腓骨颈之间腓神经的片断。通过M allory三色染色,显示神经截面中不同结缔组织成份。通过Luxol...目的了解喉返神经解剖结构的特点对于今后神经修复和器官移植的意义。方法采取6只成年狗的右侧、同长度的颈部喉返神经和坐骨神经末端分支处至腓骨颈之间腓神经的片断。通过M allory三色染色,显示神经截面中不同结缔组织成份。通过Luxol Fast B lue染色处理,显示髓鞘,以更好显示神经束内的神经纤维的轮廓。电镜染色切片,以观察喉返神经与腓神经神经束内神经纤维的情况。应用同济大学HPIAS-1000高清晰度彩色病理图文分析系统镜下采集图像并分析。应用统计学软件SPSS10.0进行统计学处理。采用两组配对t检验,P<0.05为显著差异。结果喉返神经及腓神经各自外膜面积占整个神经横截面的比例分别为(x-±s)(0.8995±7.43200)e-03(0.7073±4.39024)e-02。P<0.05具有显著差异。喉返神经及腓神经各自神经束面积占整个神经横截面的比例分别为(0.1005±7.43200)e-03(0.2927±4.39024)e-02。P<0.05具有显著差异。喉返神经及腓神经各自脂肪面积占神经外膜的比例分别为(0.7089±4.31622)e-02;(0.182683±7.62054)e-02。P<0.05具有显著差异。喉返神经及腓神经神经束内神经纤维面积的比例分别为(0.5616±2.67366)e-02(0.4743±1.51365)e-02,P<0.05具有显著差异。结论喉返神经的解剖结构与腓神经有明显的不同:喉返神经的微观解剖结构更不利于神经的修复,因此在选择神经修复方法及不同种类神经与喉返神经端端吻合或端侧吻合时,应对喉返神经的解剖结构给予重视。展开更多
基金supported by the Jilin Provincial Science & Technology Development Project Fund of China,No.20150311038YY
文摘The repair of peripheral nerve injury after complete amputation is difficult,and even with anastomosis,the rapid recovery of nerve function remains challenging.Curcumin,extracted from plants of the genus Curcuma,has been shown to have anti-oxidant and anti-inflammatory properties and to improve sciatic nerve crush injury in rats.Here,we determined whether curcumin had neuroprotective effects following complete peripheral nerve amputation injury.BALB/c mice underwent complete sciatic nerve amputation,followed by an immediate epineurium anastomosis.Mice were intragastrically administered curcumin at doses of 40（high）,20（moderate）,and 10 mg/kg/d（low） for 1 week.We found that myelin in the mice of the high- and moderate-dose curcumin groups appeared with regular shape,uniform thickness,clear boundary,and little hyperplasia surrounding the myelin.High and moderate doses of curcumin markedly improved both action potential amplitude of the sciatic nerves and the conduction velocity of the corresponding motor neurons,and upregulated m RNA and protein expression of S100,a marker for Schwann cell proliferation,in L4–6 spinal cord segments.These results suggest that curcumin is effective in promoting the repair of complete sciatic nerve amputation injury and that the underlying mechanism may be associated with upregulation of S100 expression.
文摘A number of studies have shown how to eliminate the misorientated docking of the peripheral nerve bundle in the traditional epineurium or perineurium anastomosis, thus avoiding neuroma formation and axonal outgrowth from the coaptation sites, and seriously hindering neural function recovery. Based on the ＂peripheral nerve seJective regeneration theory＂, this experiment was designed to investigate the feasibility and benefits of a new small gap anastomosis repairing peripheral nerve rupture, by scissoring and sleeve jointing an autologous epineufium, in the proximal stump of the nerve, a 1 mm-long epineurium was annularly separated and removed, while a 3 mm-long epineurium was longitudinally incised in the distal stump after the epineurium was dissociated from proximal to distal. The epineuria of the two stumps and the longitudinal incision were sutured, leaving a 2 mm gap between the two nerve stumps. Results show that the experimental rats quickly recovered autonomic activities, and there were minimal adhesions at the outer surface of the epineudal tube to the surrounding tissue. The morphologJc changes to the sciatic nerve showed that connective tissue hyperplasia of the small gaps was significantly reduced, and nerve fibers were arranged orderly. No such changes were observed in the neurorrhaphy in situ group. Thus, the experiment confirmed that the new small gap anastomosis to repair peripheral nerve rupture by scissoring and sleeve jointing autologous epineurium is feasible, and that it is superior to epineurium neurorrhaphy in situ.
文摘目的了解喉返神经解剖结构的特点对于今后神经修复和器官移植的意义。方法采取6只成年狗的右侧、同长度的颈部喉返神经和坐骨神经末端分支处至腓骨颈之间腓神经的片断。通过M allory三色染色,显示神经截面中不同结缔组织成份。通过Luxol Fast B lue染色处理,显示髓鞘,以更好显示神经束内的神经纤维的轮廓。电镜染色切片,以观察喉返神经与腓神经神经束内神经纤维的情况。应用同济大学HPIAS-1000高清晰度彩色病理图文分析系统镜下采集图像并分析。应用统计学软件SPSS10.0进行统计学处理。采用两组配对t检验,P<0.05为显著差异。结果喉返神经及腓神经各自外膜面积占整个神经横截面的比例分别为(x-±s)(0.8995±7.43200)e-03(0.7073±4.39024)e-02。P<0.05具有显著差异。喉返神经及腓神经各自神经束面积占整个神经横截面的比例分别为(0.1005±7.43200)e-03(0.2927±4.39024)e-02。P<0.05具有显著差异。喉返神经及腓神经各自脂肪面积占神经外膜的比例分别为(0.7089±4.31622)e-02;(0.182683±7.62054)e-02。P<0.05具有显著差异。喉返神经及腓神经神经束内神经纤维面积的比例分别为(0.5616±2.67366)e-02(0.4743±1.51365)e-02,P<0.05具有显著差异。结论喉返神经的解剖结构与腓神经有明显的不同:喉返神经的微观解剖结构更不利于神经的修复,因此在选择神经修复方法及不同种类神经与喉返神经端端吻合或端侧吻合时,应对喉返神经的解剖结构给予重视。
