Enhanced oil recovery （EOR） by alkaline flooding for conventional oils has been extensively studied. For heavy oils, investigations are very limited due to the unfavorable mobility ratio between the water and oil ph...Enhanced oil recovery （EOR） by alkaline flooding for conventional oils has been extensively studied. For heavy oils, investigations are very limited due to the unfavorable mobility ratio between the water and oil phases. In this study, the displacement mechanisms of alkaline flooding for heavy oil EOR are investigated by conducting flood tests in a micromodel. Two different displacement mechanisms are observed for enhancing heavy oil recovery. One is in situ water-in-oil （W/O） emulsion formation and partial wettability alteration. The W/O emulsion formed during the injection of alkaline solution plugs high permeability water channels, and pore walls are altered to become partially oil-wetted, leading to an improvement in sweep efficiency and high tertiary oil recovery. The other mechanism is the formation of an oil-in-water （O/W） emulsion. Heavy oil is dispersed into the water phase by injecting an alkaline solution containing a very dilute surfactant. The oil is then entrained in the water phase and flows out of the model with the water phase.展开更多
Cancer cells could be eradicated by promoting generation of excessive intracellular reactive oxygen species(ROS)via emerging nanomedicines.However,tumor heterogeneity and poor penetration of nanomedicines often lead t...Cancer cells could be eradicated by promoting generation of excessive intracellular reactive oxygen species(ROS)via emerging nanomedicines.However,tumor heterogeneity and poor penetration of nanomedicines often lead to diverse levels of ROS production in the tumor site,and ROS at a low level promote tumor cell growth,thus diminishing the therapeutic effect of these nanomedicines.Herein,we construct an amphiphilic and block polymer-dendron conjugate-derived nanomedicine(Lap@pOEGMA-b-p(GFLG-Dendron-Ppa),GFLG-DP/Lap NPs)that incorporates a photosensitizer,Pyropheophorbide a(Ppa),for ROS therapy and Lapatinib(Lap)for molecular targeted therapy.Lap,an epidermal growth factor receptor(EGFR)inhibitor that plays a role in inhibiting cell growth and proliferation,is hypothesized to synergize with ROS therapy for effectively killing cancer cells.Our results suggest that the enzyme-sensitive polymeric conjugate,pOEGMA-b-p(GFLG-Dendron-Ppa)(GFLG-DP),releases in response to cathepsin B(CTSB)after entering the tumor tissue.Dendritic-Ppa has a strong adsorption capacity to tumor cell membranes,which promotes efficient penetration and long-term retention.Lap can also be efficiently delivered to internal tumor cells to play its role due to the increased vesicle activity.Laser irradiation of Ppa-containing tumor cells results in production of intracellular ROS that is sufficient for inducing cell apoptosis.Meanwhile,Lap efficiently inhibits proliferation of remaining viable cells even in deep tumor regions,thus generating a significant synergistic anti-tumor therapeutic effect.This novel strategy can be extended to the development of efficient membrane lipid-based therapies to effectively combat tumors.展开更多
The lateral flow immuno assay (LFIA) has emerged as a powerful tool for rapid scree ning owing to its simplicity and flexibility for detection of various biomarkers. However, conventional LFIA strips have several disa...The lateral flow immuno assay (LFIA) has emerged as a powerful tool for rapid scree ning owing to its simplicity and flexibility for detection of various biomarkers. However, conventional LFIA strips have several disadvantages, including limits in quantitative analysis and low sensitivity. Here we developed a novel surface-e nhanced Rama n scatteri ng LFIA based on non spherical gap-e nhanced Raman tags (GERTs), with Raman molecules (RMs) embedded in a 1-nm gap between Au nanorod core and Au shell. Such tags have a strong and uniform surface-enhanced Rama n scattering (SERS) resp on se, an order of mag nitude higher than that of other comm on SERS tags such as Au nano rods, nano stars, Au nano shells with surface-adsorbed RMs, or spherical GERTs with embedded RMs. The feasibility of the tags was dem on strated by the semiqua ntitative and sen sitive detecti on of the heart disease biomarker cardiac tropo nin I (cTnI). GERTs were conjugated with mono clonal antibodies and used for LFIA in the same way as ordinary functionalized colloidal gold. The presenee of the target antigen, cTnI, was identified by Raman microscopy mapping of the test zone. With the SERS-based LFIA, the limit of cTnI detection was about 0.1 ng/mL. This value is within the diagnostic range of cTnI in the blood serum of patients with heart infarction and is 30 times lower than that of the colorimetric LFIA test using the same antibodies and either GERTs or colloidal gold as labels.展开更多
Underground non-Darcy fluid flow has been observed and investigated for decades in the petroleum industry. It is deduced by analogy that the fluid flow in enhanced geothermal system (EGS) heat reservoirs may also be i...Underground non-Darcy fluid flow has been observed and investigated for decades in the petroleum industry. It is deduced by analogy that the fluid flow in enhanced geothermal system (EGS) heat reservoirs may also be in the non-Darcy regime under some conditions. In this paper, a transient 3D model was presented, taking into consideration the non-Darcy fluid flow in EGS heat reservoirs, to simulate the EGS long-term heat extraction process. Then, the non-Darcy flow behavior in water- and supercritical CO2 (SCCO2)-based EGSs was simulated and discussed. It is found that non-Darcy effects decrease the mass flow rate of the fluid injected and reduce the heat extraction rate of EGS as a flow resistance in addition to the Darcy resistance which is imposed to the seepage flow in EGS heat reservoirs. Compared with the water-EGS, the SCCO2-EGS are more prone to experiencing much stronger non-Darcy flow due to the much larger mobility of the SCCO2. The non-Darcy flow in SCCO2- EGSs may thus greatly reduce their heat extraction performance. Further, a criterion was analyzed and proposed to judge the onset of the non-Darcy flow in EGS heat reservoirs. The fluid flow rate and the initial thermal state of the reservoir were taken and the characteristic Forchheimer number of an EGS was calculated. If the calculated Forchheimer number is larger than 0.2, the fluid flow in EGS heat reservoirs experiences non-negligible non-Darcy flow characteristic.展开更多
以构建的三级生物沸石人工湿地处理分散养猪冲洗水厌氧出水,考察了湿地的运行性能、污染物随时间变化以及生物沸石层ORP分布情况.结果表明,三级生物沸石人工湿地可高效地去除分散养猪冲洗水厌氧出水的COD、N和P,且耐NH_4^+-N冲击负荷....以构建的三级生物沸石人工湿地处理分散养猪冲洗水厌氧出水,考察了湿地的运行性能、污染物随时间变化以及生物沸石层ORP分布情况.结果表明,三级生物沸石人工湿地可高效地去除分散养猪冲洗水厌氧出水的COD、N和P,且耐NH_4^+-N冲击负荷.湿地水力负荷为0.047 m^3·(m^2·d)^(-1)时,COD、NH_4^+-N和TN(平均质量浓度分别为477.7、155.3和176.4mg·L^(-1))主要在湿地一区被去除,平均去除率分别为80.6%、55.3%和58.1%.生物沸石强化硝化作用明显,硝化产物主要为硝酸盐,湿地一区、二区和三区的NO_3^--N产生质量浓度分别为85.85、91.06和82.41 mg·L^(-1),一区沸石层产生的硝酸盐可被其下部砖渣层微生物利用水中剩余有机物为底物,通过反硝化途径去除.TP主要靠砖渣吸附去除,微生物的作用相对较小.三级湿地沸石层复氧效果均较好,大部分ORP值都保持在400 m V以上.展开更多
Carbonate reservoirs in China have the characteristics of diversified accumulation pattern, complex structure and varying reservoir conditions. Concerning these characteristics, this article tracks the technical break...Carbonate reservoirs in China have the characteristics of diversified accumulation pattern, complex structure and varying reservoir conditions. Concerning these characteristics, this article tracks the technical breakthroughs and related practices since the 1950 s, summarizes the developed theory and technologies of carbonate reservoir development, analyzes their adaptability and problems, and proposes their development trend. The following theory and technologies have come into being:(1) carbonate reservoir formation mechanisms and compound flow mechanisms in complex medium;(2) reservoir identification and description technologies based on geophysics and discrete fracture-vuggy modeling method;(3) well testing analysis technology and numerical simulation method of coupling free flow and porous media flow;(4) enhanced oil recovery techniques for nitrogen single well huff and puff, and water flooding development techniques with well pattern design in spatial structure, changed intensity water injection, water plugging and channel blocking as the core;(5) drilling and completion techniques, acid fracturing techniques and its production increasing techniques. To realize the efficient development of carbonate oil and gas reservoirs, researches in four aspects need to be done:(1) complex reservoir description technology with higher accuracy;(2) various enhanced oil recovery techniques;(3) improving the drilling method and acid fracturing method for ultra-deep carbonate reservoir and significantly cutting engineering cost;(4) strengthening the technological integration of information, big data, cloud computation, and artificial intelligence in oilfield development to realize the smart development of oilfield.展开更多
A previous study showed that the thermal performance of the X-lattice cored corrugated honeycomb(XCCH)is better than that of most other periodic cellular materials(PCMs).To further improve the thermal performance of t...A previous study showed that the thermal performance of the X-lattice cored corrugated honeycomb(XCCH)is better than that of most other periodic cellular materials(PCMs).To further improve the thermal performance of the XCCH,the effects of different ripple amplitudes(i.e.,a=0.5,0.7 and 1.0)on the characteristics of the flow and heat transfer are numerically investigated by thorough comparisons.In terms of the flow characteristics,with the increase of ripple amplitude,the vortex interaction in the channel becomes stronger,which results in evident increase of kinetic energy of turbulence at the boundary of vortex and reduction in the turbulent kinetic energy dissipation.As far as the heat transfer is concerned,within the Reynolds number range of 3696–7436,the heat transfer increases with the increase of ripple amplitude.The overall Nusselt number of the XCCH with a=1.0 is 15.7%higher than that with a=0.5.Within the corresponding range of pumping power,the thermal performance of the XCCH with a=1.0 is up to 7%higher than that with a=0.5 at relatively higher Reynolds numbers.展开更多
The application of vortex generators in tube-finned heat exchangers is very universal.The vortex generators can generate secondary flow,and as we all know secondary flow can obviously strengthen heat transfer.To use v...The application of vortex generators in tube-finned heat exchangers is very universal.The vortex generators can generate secondary flow,and as we all know secondary flow can obviously strengthen heat transfer.To use vortex generators much more efficiently in the circle tube-finned heat exchangers,the intensity correlation study between secondary flow and heat transfer is needed.22 different structures of circle tube-finned heat exchangers were numerically studied,including the plain fin cases and the cases with vortex generators.In addition,the influence of fin spacing,transverse and longitudinal tube pitch,heights and attack angle of vortex generators,positions of vortex generators and shape of vortex generators on heat transfer and fluid flow are studied,too.The non-dimensional parameter Se is applied to quantify the secondary flow intensity.The results show that Se can describe the secondary flow intensity very well.There is very close corresponding relationship between overall averaged Nu and volumetrically averaged Se for all the researched cases and the relational expression is obtained.However,there is no one-to-one correlation not only between Re and f but also between volumetrically averaged Se and f for all the studied cases.展开更多
As a promising enhanced gas recovery technique,CO_(2)huff-n-puff has attracted great attention recently.However,hydraulic fracture deformation hysteresis is rarely considered,and its effect on CO_(2)huff-n-puff perfor...As a promising enhanced gas recovery technique,CO_(2)huff-n-puff has attracted great attention recently.However,hydraulic fracture deformation hysteresis is rarely considered,and its effect on CO_(2)huff-n-puff performance is not well understood.In this study,we present a fully coupled multi-component flow and geomechanics model for simulating CO_(2)huff-n-puff in shale gas reservoirs considering hydraulic fracture deformation hysteresis.Specifically,a shale gas reservoir after hydraulic fracturing is modeled using an efficient hybrid model incorporating an embedded discrete fracture model(EDFM),multiple porosity model,and single porosity model.In flow equations,Fick’s law,extended Langmuir isotherms,and the Peng-Robinson equation of state are used to describe the molecular diffusion,multi-component adsorption,and gas properties,respectively.In relation to geomechanics,a path-dependent constitutive law is applied for the hydraulic fracture deformation hysteresis.The finite volume method(FVM)and the stabilized extended finite element method(XFEM)are applied to discretize the flow and geomechanics equations,respectively.We then solve the coupled model using the fixed-stress split iterative method.Finally,we verify the presented method using several numerical examples,and apply it to investigate the effect of hydraulic fracture deformation hysteresis on CO_(2)huff-n-puff performance in a 3D shale gas reservoir.Numerical results show that hydraulic fracture deformation hysteresis has some negative effects on CO_(2)huff-n-puff performance.The effects are sensitive to the initial conductivity of hydraulic fracture,production pressure,starting time of huff-n-puff,injection pressure,and huff-n-puff cycle number.展开更多
In this paper, we solve Beck and Wayne's conjecture on the optimal enhanced dissipation rate for the 2-D linearized Navier-Stokes equations around the bar state called the Kolmogorov flow by developing the hypocoe...In this paper, we solve Beck and Wayne's conjecture on the optimal enhanced dissipation rate for the 2-D linearized Navier-Stokes equations around the bar state called the Kolmogorov flow by developing the hypocoercivity method introduced by Villani(2009).展开更多
The fracture characteristics of a heat reservoir are of critical importance to enhanced geothennal systems,which can be investigated by theoretical modeling.This paper presents the development of a novel flow.Fesistor...The fracture characteristics of a heat reservoir are of critical importance to enhanced geothennal systems,which can be investigated by theoretical modeling.This paper presents the development of a novel flow.Fesistor network model to describe the hydraulic processes in heat reservoirs.The fractures in the reservoir are simplified by using flow resistors and the typically complicated fracture network of the heat reservoir is converted into a flowresistor network with a reasonably simple pattern.For heat reservoirs with various fracture configurations,the corresponding flow-resistor networks are identical in terms of framework though the networks may have difftrent section numbers and the flow resistors may have different values.In this paper,numerous cases of different section numbers and resistor values are calculated and the results indicate that the total number of flow resistances between the injection and production wells is primarily determined by the number of fractures in the reservoir.It is also observed that a linear dependence of the total flow resistance on the number of fractures and the relation is obtained by the best fit of the calculation results.Besides,it perfbmis a case study dealing with the Soultz enhanced geothermal system(EGS).In addition,the fracture numbers underneath specific well systems are derived.The results provide in sight on the tortuosity of the flow path betwee n different wells.展开更多
Phosphorylation of tau at Ser(396,404)(p-tau^(396,404))is one of the earliest phosphorylation events,plasma p-tau^(396,404) level appears to be a potentially promising biomarker of Alzheimer’s disease(AD).The low abu...Phosphorylation of tau at Ser(396,404)(p-tau^(396,404))is one of the earliest phosphorylation events,plasma p-tau^(396,404) level appears to be a potentially promising biomarker of Alzheimer’s disease(AD).The low abundance and easy degradation of p-tau in the plasma make the lateral flow assay(LFA)a suitable choice for point-of-care detection of plasma p-tau^(396,404) levels.Herein,based on our screening of a pair of p-tau^(396,404)-specific antibodies,we developed a colorimetric and surface-enhanced Raman scattering(SERS)dual-readout LFA for the rapid,highly sensitive,robust detection of plasma p-tau^(396,404) levels.This LFA realized a detection limit of 60 pg/mL by the naked eye or 3.8 pg/mL by SERS without cross-reacting with other tau species.More importantly,LFA rapidly and accurately differentiated AD patients from healthy controls,suggesting that it has the potential for clinical point-of-care application in AD diagnosis.This dual-readout LFA has the advantages of simple operation,rapid,ultra-sensitive detection,providing a new way for early AD diagnosis and intervention,especially in primary and community AD screening.展开更多
To make clear the precise hemodynamic mechanism underlying the anti-atherogenesis benefit of enhanced external couterpulsation(EECP) treatment, and to investigate the proper role of some important hemodynamic factors ...To make clear the precise hemodynamic mechanism underlying the anti-atherogenesis benefit of enhanced external couterpulsation(EECP) treatment, and to investigate the proper role of some important hemodynamic factors during the atherosclerotic progress, a comprehensive study combining long-term animal experiment and numerical solving was conducted in this paper. An experimentally induced hypercholesterolemic porcine model was developed and the chronic EECP intervention was subjected. Basic hemodynamic measurement was performed in vivo, as well as the arterial endothelial samples were extracted for physiological examination. Meanwhile, a numerical model was introduced to solve the complex hemodynamic factors such as WSS and OSI. The results show that EECP treatment resulted in significant increase of the instant levels of arterial WSS, blood pressure, and OSI. During EECP treatment, the instant OSI level of the common carotid arteries over cardiac cycles raised to a mean value of 8.58 ×10-2±2.13 ×10-2. Meanwhile, the chronic intervention of EECP treatment significantly reduced the atherosclerotic lesions in abdominal aortas and the endothelial cellular adherence. The present study suggests that the unique blood flow pattern induced by EECP treatment and the augmentation of WSS level in cardiac cycles may be the most important hemodynamic mechanism that contribute to its anti-atherogenesis effect. And as one of the indices that cause great concern in current hemodynamic study, OSI may not play a key role during the initiation of atherosclerosis.展开更多
基金the Petroleum Technology Research Center(PTRC)in Regina,Saskatchewan,Canadathe Natural Sciences and Engineering Research Council of Canada(NSERC)for their financial support of this work
文摘Enhanced oil recovery （EOR） by alkaline flooding for conventional oils has been extensively studied. For heavy oils, investigations are very limited due to the unfavorable mobility ratio between the water and oil phases. In this study, the displacement mechanisms of alkaline flooding for heavy oil EOR are investigated by conducting flood tests in a micromodel. Two different displacement mechanisms are observed for enhancing heavy oil recovery. One is in situ water-in-oil （W/O） emulsion formation and partial wettability alteration. The W/O emulsion formed during the injection of alkaline solution plugs high permeability water channels, and pore walls are altered to become partially oil-wetted, leading to an improvement in sweep efficiency and high tertiary oil recovery. The other mechanism is the formation of an oil-in-water （O/W） emulsion. Heavy oil is dispersed into the water phase by injecting an alkaline solution containing a very dilute surfactant. The oil is then entrained in the water phase and flows out of the model with the water phase.
基金supported by National Natural Science Foundation of China(52073193,51873120,81621003,52203182,32071284)National Key Research and Development Program of China(2022YFC2009900)+2 种基金1·3·5 Project for Disciplines of Excellence,West China Hospital,Sichuan University(ZYJC21013)Research Funds in West China Hospital of Sichuan University(2020HXBH072)China Postdoctoral Science Foundation(2019TQ0220,2022M712225,2022T150446).
文摘Cancer cells could be eradicated by promoting generation of excessive intracellular reactive oxygen species(ROS)via emerging nanomedicines.However,tumor heterogeneity and poor penetration of nanomedicines often lead to diverse levels of ROS production in the tumor site,and ROS at a low level promote tumor cell growth,thus diminishing the therapeutic effect of these nanomedicines.Herein,we construct an amphiphilic and block polymer-dendron conjugate-derived nanomedicine(Lap@pOEGMA-b-p(GFLG-Dendron-Ppa),GFLG-DP/Lap NPs)that incorporates a photosensitizer,Pyropheophorbide a(Ppa),for ROS therapy and Lapatinib(Lap)for molecular targeted therapy.Lap,an epidermal growth factor receptor(EGFR)inhibitor that plays a role in inhibiting cell growth and proliferation,is hypothesized to synergize with ROS therapy for effectively killing cancer cells.Our results suggest that the enzyme-sensitive polymeric conjugate,pOEGMA-b-p(GFLG-Dendron-Ppa)(GFLG-DP),releases in response to cathepsin B(CTSB)after entering the tumor tissue.Dendritic-Ppa has a strong adsorption capacity to tumor cell membranes,which promotes efficient penetration and long-term retention.Lap can also be efficiently delivered to internal tumor cells to play its role due to the increased vesicle activity.Laser irradiation of Ppa-containing tumor cells results in production of intracellular ROS that is sufficient for inducing cell apoptosis.Meanwhile,Lap efficiently inhibits proliferation of remaining viable cells even in deep tumor regions,thus generating a significant synergistic anti-tumor therapeutic effect.This novel strategy can be extended to the development of efficient membrane lipid-based therapies to effectively combat tumors.
文摘The lateral flow immuno assay (LFIA) has emerged as a powerful tool for rapid scree ning owing to its simplicity and flexibility for detection of various biomarkers. However, conventional LFIA strips have several disadvantages, including limits in quantitative analysis and low sensitivity. Here we developed a novel surface-e nhanced Rama n scatteri ng LFIA based on non spherical gap-e nhanced Raman tags (GERTs), with Raman molecules (RMs) embedded in a 1-nm gap between Au nanorod core and Au shell. Such tags have a strong and uniform surface-enhanced Rama n scattering (SERS) resp on se, an order of mag nitude higher than that of other comm on SERS tags such as Au nano rods, nano stars, Au nano shells with surface-adsorbed RMs, or spherical GERTs with embedded RMs. The feasibility of the tags was dem on strated by the semiqua ntitative and sen sitive detecti on of the heart disease biomarker cardiac tropo nin I (cTnI). GERTs were conjugated with mono clonal antibodies and used for LFIA in the same way as ordinary functionalized colloidal gold. The presenee of the target antigen, cTnI, was identified by Raman microscopy mapping of the test zone. With the SERS-based LFIA, the limit of cTnI detection was about 0.1 ng/mL. This value is within the diagnostic range of cTnI in the blood serum of patients with heart infarction and is 30 times lower than that of the colorimetric LFIA test using the same antibodies and either GERTs or colloidal gold as labels.
基金This work was supported by the National Key R&D Program of China (2018YFB1501804)the Strategic Priority Research Program of Chinese Academy of Sciences (XDA21060700)+1 种基金the National Natural Science Foundation of China (Grant No. 41702256)the Natural Science Foundation of Guangdong Province (2017A030310328).
文摘Underground non-Darcy fluid flow has been observed and investigated for decades in the petroleum industry. It is deduced by analogy that the fluid flow in enhanced geothermal system (EGS) heat reservoirs may also be in the non-Darcy regime under some conditions. In this paper, a transient 3D model was presented, taking into consideration the non-Darcy fluid flow in EGS heat reservoirs, to simulate the EGS long-term heat extraction process. Then, the non-Darcy flow behavior in water- and supercritical CO2 (SCCO2)-based EGSs was simulated and discussed. It is found that non-Darcy effects decrease the mass flow rate of the fluid injected and reduce the heat extraction rate of EGS as a flow resistance in addition to the Darcy resistance which is imposed to the seepage flow in EGS heat reservoirs. Compared with the water-EGS, the SCCO2-EGS are more prone to experiencing much stronger non-Darcy flow due to the much larger mobility of the SCCO2. The non-Darcy flow in SCCO2- EGSs may thus greatly reduce their heat extraction performance. Further, a criterion was analyzed and proposed to judge the onset of the non-Darcy flow in EGS heat reservoirs. The fluid flow rate and the initial thermal state of the reservoir were taken and the characteristic Forchheimer number of an EGS was calculated. If the calculated Forchheimer number is larger than 0.2, the fluid flow in EGS heat reservoirs experiences non-negligible non-Darcy flow characteristic.
文摘以构建的三级生物沸石人工湿地处理分散养猪冲洗水厌氧出水,考察了湿地的运行性能、污染物随时间变化以及生物沸石层ORP分布情况.结果表明,三级生物沸石人工湿地可高效地去除分散养猪冲洗水厌氧出水的COD、N和P,且耐NH_4^+-N冲击负荷.湿地水力负荷为0.047 m^3·(m^2·d)^(-1)时,COD、NH_4^+-N和TN(平均质量浓度分别为477.7、155.3和176.4mg·L^(-1))主要在湿地一区被去除,平均去除率分别为80.6%、55.3%和58.1%.生物沸石强化硝化作用明显,硝化产物主要为硝酸盐,湿地一区、二区和三区的NO_3^--N产生质量浓度分别为85.85、91.06和82.41 mg·L^(-1),一区沸石层产生的硝酸盐可被其下部砖渣层微生物利用水中剩余有机物为底物,通过反硝化途径去除.TP主要靠砖渣吸附去除,微生物的作用相对较小.三级湿地沸石层复氧效果均较好,大部分ORP值都保持在400 m V以上.
基金Supported by the China National Science and Technology Major Project(2016ZX05014)
文摘Carbonate reservoirs in China have the characteristics of diversified accumulation pattern, complex structure and varying reservoir conditions. Concerning these characteristics, this article tracks the technical breakthroughs and related practices since the 1950 s, summarizes the developed theory and technologies of carbonate reservoir development, analyzes their adaptability and problems, and proposes their development trend. The following theory and technologies have come into being:(1) carbonate reservoir formation mechanisms and compound flow mechanisms in complex medium;(2) reservoir identification and description technologies based on geophysics and discrete fracture-vuggy modeling method;(3) well testing analysis technology and numerical simulation method of coupling free flow and porous media flow;(4) enhanced oil recovery techniques for nitrogen single well huff and puff, and water flooding development techniques with well pattern design in spatial structure, changed intensity water injection, water plugging and channel blocking as the core;(5) drilling and completion techniques, acid fracturing techniques and its production increasing techniques. To realize the efficient development of carbonate oil and gas reservoirs, researches in four aspects need to be done:(1) complex reservoir description technology with higher accuracy;(2) various enhanced oil recovery techniques;(3) improving the drilling method and acid fracturing method for ultra-deep carbonate reservoir and significantly cutting engineering cost;(4) strengthening the technological integration of information, big data, cloud computation, and artificial intelligence in oilfield development to realize the smart development of oilfield.
基金supported by the National Natural Science Foundation of China (Grant No.51806176)the Fundamental Research Funds for the Central Universities (Grant No.30922010914)。
文摘A previous study showed that the thermal performance of the X-lattice cored corrugated honeycomb(XCCH)is better than that of most other periodic cellular materials(PCMs).To further improve the thermal performance of the XCCH,the effects of different ripple amplitudes(i.e.,a=0.5,0.7 and 1.0)on the characteristics of the flow and heat transfer are numerically investigated by thorough comparisons.In terms of the flow characteristics,with the increase of ripple amplitude,the vortex interaction in the channel becomes stronger,which results in evident increase of kinetic energy of turbulence at the boundary of vortex and reduction in the turbulent kinetic energy dissipation.As far as the heat transfer is concerned,within the Reynolds number range of 3696–7436,the heat transfer increases with the increase of ripple amplitude.The overall Nusselt number of the XCCH with a=1.0 is 15.7%higher than that with a=0.5.Within the corresponding range of pumping power,the thermal performance of the XCCH with a=1.0 is up to 7%higher than that with a=0.5 at relatively higher Reynolds numbers.
基金supported by the National Natural Science Foundation of China(Nos.51868035,51866006,51468028)Foundation of a Hundred Youth Talents Training Program of Lanzhou Jiaotong Universitythe Science and Technology Plan of Gansu Province(No.18JR3RA121).
文摘The application of vortex generators in tube-finned heat exchangers is very universal.The vortex generators can generate secondary flow,and as we all know secondary flow can obviously strengthen heat transfer.To use vortex generators much more efficiently in the circle tube-finned heat exchangers,the intensity correlation study between secondary flow and heat transfer is needed.22 different structures of circle tube-finned heat exchangers were numerically studied,including the plain fin cases and the cases with vortex generators.In addition,the influence of fin spacing,transverse and longitudinal tube pitch,heights and attack angle of vortex generators,positions of vortex generators and shape of vortex generators on heat transfer and fluid flow are studied,too.The non-dimensional parameter Se is applied to quantify the secondary flow intensity.The results show that Se can describe the secondary flow intensity very well.There is very close corresponding relationship between overall averaged Nu and volumetrically averaged Se for all the researched cases and the relational expression is obtained.However,there is no one-to-one correlation not only between Re and f but also between volumetrically averaged Se and f for all the studied cases.
基金This work is supported by the National Natural Sci‐ence Foundation of China(Nos.52004321,52034010,and 12131014)the Natural Science Foundation of Shandong Province,China(No.ZR2020QE116)the Fundamental Research Funds for the Central Universities,China(Nos.20CX06025A and 21CX06031A).
文摘As a promising enhanced gas recovery technique,CO_(2)huff-n-puff has attracted great attention recently.However,hydraulic fracture deformation hysteresis is rarely considered,and its effect on CO_(2)huff-n-puff performance is not well understood.In this study,we present a fully coupled multi-component flow and geomechanics model for simulating CO_(2)huff-n-puff in shale gas reservoirs considering hydraulic fracture deformation hysteresis.Specifically,a shale gas reservoir after hydraulic fracturing is modeled using an efficient hybrid model incorporating an embedded discrete fracture model(EDFM),multiple porosity model,and single porosity model.In flow equations,Fick’s law,extended Langmuir isotherms,and the Peng-Robinson equation of state are used to describe the molecular diffusion,multi-component adsorption,and gas properties,respectively.In relation to geomechanics,a path-dependent constitutive law is applied for the hydraulic fracture deformation hysteresis.The finite volume method(FVM)and the stabilized extended finite element method(XFEM)are applied to discretize the flow and geomechanics equations,respectively.We then solve the coupled model using the fixed-stress split iterative method.Finally,we verify the presented method using several numerical examples,and apply it to investigate the effect of hydraulic fracture deformation hysteresis on CO_(2)huff-n-puff performance in a 3D shale gas reservoir.Numerical results show that hydraulic fracture deformation hysteresis has some negative effects on CO_(2)huff-n-puff performance.The effects are sensitive to the initial conductivity of hydraulic fracture,production pressure,starting time of huff-n-puff,injection pressure,and huff-n-puff cycle number.
基金supported by National Natural Science Foundation of China (Grant No. 11425103)
文摘In this paper, we solve Beck and Wayne's conjecture on the optimal enhanced dissipation rate for the 2-D linearized Navier-Stokes equations around the bar state called the Kolmogorov flow by developing the hypocoercivity method introduced by Villani(2009).
文摘The fracture characteristics of a heat reservoir are of critical importance to enhanced geothennal systems,which can be investigated by theoretical modeling.This paper presents the development of a novel flow.Fesistor network model to describe the hydraulic processes in heat reservoirs.The fractures in the reservoir are simplified by using flow resistors and the typically complicated fracture network of the heat reservoir is converted into a flowresistor network with a reasonably simple pattern.For heat reservoirs with various fracture configurations,the corresponding flow-resistor networks are identical in terms of framework though the networks may have difftrent section numbers and the flow resistors may have different values.In this paper,numerous cases of different section numbers and resistor values are calculated and the results indicate that the total number of flow resistances between the injection and production wells is primarily determined by the number of fractures in the reservoir.It is also observed that a linear dependence of the total flow resistance on the number of fractures and the relation is obtained by the best fit of the calculation results.Besides,it perfbmis a case study dealing with the Soultz enhanced geothermal system(EGS).In addition,the fracture numbers underneath specific well systems are derived.The results provide in sight on the tortuosity of the flow path betwee n different wells.
基金the National Science and Technology Innovation 2030(Nos.2021ZD0201000 and 2021ZD0201001)the National Natural Science Foundation of China(No.81971025)the Chinese Academy of Medical Sciences(CAMS)Innovation Fund for Medical Sciences(No.2019-I2M-5-014).
文摘Phosphorylation of tau at Ser(396,404)(p-tau^(396,404))is one of the earliest phosphorylation events,plasma p-tau^(396,404) level appears to be a potentially promising biomarker of Alzheimer’s disease(AD).The low abundance and easy degradation of p-tau in the plasma make the lateral flow assay(LFA)a suitable choice for point-of-care detection of plasma p-tau^(396,404) levels.Herein,based on our screening of a pair of p-tau^(396,404)-specific antibodies,we developed a colorimetric and surface-enhanced Raman scattering(SERS)dual-readout LFA for the rapid,highly sensitive,robust detection of plasma p-tau^(396,404) levels.This LFA realized a detection limit of 60 pg/mL by the naked eye or 3.8 pg/mL by SERS without cross-reacting with other tau species.More importantly,LFA rapidly and accurately differentiated AD patients from healthy controls,suggesting that it has the potential for clinical point-of-care application in AD diagnosis.This dual-readout LFA has the advantages of simple operation,rapid,ultra-sensitive detection,providing a new way for early AD diagnosis and intervention,especially in primary and community AD screening.
基金Key Clinical Project from the Ministry of Healthgrant number:25400+1 种基金National Natural Science Foundation of Chinagrant number:81170272
文摘To make clear the precise hemodynamic mechanism underlying the anti-atherogenesis benefit of enhanced external couterpulsation(EECP) treatment, and to investigate the proper role of some important hemodynamic factors during the atherosclerotic progress, a comprehensive study combining long-term animal experiment and numerical solving was conducted in this paper. An experimentally induced hypercholesterolemic porcine model was developed and the chronic EECP intervention was subjected. Basic hemodynamic measurement was performed in vivo, as well as the arterial endothelial samples were extracted for physiological examination. Meanwhile, a numerical model was introduced to solve the complex hemodynamic factors such as WSS and OSI. The results show that EECP treatment resulted in significant increase of the instant levels of arterial WSS, blood pressure, and OSI. During EECP treatment, the instant OSI level of the common carotid arteries over cardiac cycles raised to a mean value of 8.58 ×10-2±2.13 ×10-2. Meanwhile, the chronic intervention of EECP treatment significantly reduced the atherosclerotic lesions in abdominal aortas and the endothelial cellular adherence. The present study suggests that the unique blood flow pattern induced by EECP treatment and the augmentation of WSS level in cardiac cycles may be the most important hemodynamic mechanism that contribute to its anti-atherogenesis effect. And as one of the indices that cause great concern in current hemodynamic study, OSI may not play a key role during the initiation of atherosclerosis.
