A stranded wires helical spring is formed of a multilayer and coaxial strand of several wires twisted together with the same direction of spiral. Compared with the conventional single wire spring, the stranded wires h...A stranded wires helical spring is formed of a multilayer and coaxial strand of several wires twisted together with the same direction of spiral. Compared with the conventional single wire spring, the stranded wires helical spring has the notable predominance in strength, damping and vibration reduction, which is usually used in aircraft engines, automatic weapons, etc. However, due to its complicated structure, the precise computation of its strength and rigidity need be a correct mathematical model, which then will be imported to finite element analysis software for solutions. Equations on solving geometric parameters, such as external diameters of strands and screw pitches of wires, are put forward in the paper. It also proposes a novel methodology on solving geometric parameters and establishing entity models of the stranded wires helical spring, which provides foundation of computing mechanical parameters by FEA. Then mathematical models on the centre line of the strand and the surface curve of each wire, after closing two ends in a spring, are proposed. Finally, geometric parameters are solved in a case study, and a 3D entity model of a spring with 3 layers and 16 wires is established, which has validated the accuracy of the proposed methodology and the 3D entity mathematical model. The method provides a new way to design stranded wire helical spring.展开更多
Blue-grained wheat derived from the hybrid Triticum aestivum L. X Thinopyrum ponticum (Podp.) Barkworth et D. R. Dewey (Agropyron elongatum (Host) P. Beauv., 2n=70). The molecular biological mechanism of the biosynthe...Blue-grained wheat derived from the hybrid Triticum aestivum L. X Thinopyrum ponticum (Podp.) Barkworth et D. R. Dewey (Agropyron elongatum (Host) P. Beauv., 2n=70). The molecular biological mechanism of the biosynthetic pathway of blue pigments in the blue grain remains unclear yet. Dihydroflavonol 4-reductase (DFR) is one of the key enzymes controlling flavonoid synthesis in anthocyanin biosynthetic pathway, and may directly participate in the formation of blue pigment in the aleurone layer of blue-grained wheat. Here we cloned a DFR cDNA (TaDFR) from the developing seeds of blue-grained wheat, and four DFR genomic DNAs from Th. ponticum (ThpDFR.t), blue-grained wheat (TaDFR.bg), white-grained offspring of light blue-grained wheat (TaDFR.wg) and Chinese Spring (2n=42) (TaDFR.csg), respectively. TaDFR cDNA encodes a 354 amino-acids polypeptide with high identity to DFR from Hordeum vulgare L. (94%), Oryza sativa L. (83%), Zea mays L.(84%). The result of cluster analysis showed that TaDFR cDNA nucleotide sequence has 100% identity with that of TaDFR.csg. The four DFR genomic DNAs have extraordinary high homology and each has three introns. The differences of the four DFR genomic DNAs mainly exist in introns. Southern blotting analysis showed that there are at least 3-5 DFR copies in wheat, the copy numbers in different color grain wheats are not significantly different. The hybridization band patterns were the same, but different from that of Th. ponticum. DFR in blue-grained wheat belongs to a DFR superfamily. Northern blotting analysis indicated that the DFR expressed in the developing seeds of both blue- and white-grained wheat at 15 d after flowering (DAF), the mRNA levels of DFR reached the highest at 18 DAF, then declined quickly and disappeared at 33 DAF But the expression levels in blue-grained seeds were higher than that in white grain at the same seed developing stages. DFR transcripts accumulated in young leaves, and leaf sheaths of blue- and white-grained wheat and Th ponticum, but not detected in roo展开更多
Farnesyl dlphosphate synthase （FPS; EC 2.5.1.10） catalyzes the production of 15-carbon farnesyl dlphosphate which Is a branch-point Intermediate for many terpenoids. This reaction Is considered to be a ratelimiting ...Farnesyl dlphosphate synthase （FPS; EC 2.5.1.10） catalyzes the production of 15-carbon farnesyl dlphosphate which Is a branch-point Intermediate for many terpenoids. This reaction Is considered to be a ratelimiting step In terpenold biosynthesis. Here we report for the first time the cloning of a new full-length cDNA encoding farnesyl dlphosphate synthase from a gymnosperm plant species, Taxus media Rehder, designated as TmFPS1. The full-length cDNA of TmFPS1 （GenBank accession number： AY461811） was 1 464 bp with a 1 056-bp open reading frame encoding a 351-amino acid polypeptlde with a calculated molecular weight of 40.3 kDa and a theoretical pl of 5.07. Biolnformatlc analysis revealed that TmFPS1 contained all five conserved domains of prenyltransferases, and showed homology to other FPSs of plant origin. Phylogenetlc analysis showed that farnesyl dlphosphate synthases can be divided Into two groups： one of prokaryotic origin and the other of eukaryotic origin. TmFPS1 was grouped with FPSs of plant origin. Homologybased structural modeling showed that TmFPS1 had the typical spatial structure of FPS, whose most prominent structural feature Is the arrangement of 13 core helices around a large central cavity In which the catalytic reaction takes place. Our blolnformatic analysis strongly suggests that TmFPS1 is a functional gene. Southern blot analysis revealed that TmFPS1 belongs to a small FPSgene family in T. media. Northern blot analysis indicated that TmFPS1 is expressed in all tested tissues, Including the needles, stems and roots of T. media. Subsequently, functional complementatlon with TmFPS1 in a FPS-deflclent mutant yeast demonstrated that TmFPS1 did encode farnesyl dlphosphate synthase, which rescued the yeast mutant. This study will be helpful In future Investigations aiming at understanding the detailed role of FPS In terpenold biosynthesis flux control at the molecular genetic level.展开更多
The grating ends bonding fiber Bragg grating(FBG)sensor has been widely used in sensor packages such as substrate type and clamp type for health monitoring of large structures.However,owing to the shear deformation of...The grating ends bonding fiber Bragg grating(FBG)sensor has been widely used in sensor packages such as substrate type and clamp type for health monitoring of large structures.However,owing to the shear deformation of the adhesive layer of FBG,the strain measured by FBG is often different from the strain of actual matrix,which causes strain measurement errors.This investigation aims at improving the measurement accuracy of strain for the grating ends surface-bonded FBG.To fulfill this objective,a strain transfer equation of the grating ends bonding FBG is derived,and a theoretical model of the average strain transfer from the matrix to the optical fiber is developed.Moreover,parameters that influence the average strain transfer rate from the matrix to the optical fiber are analyzed.A selection scheme of bonding parameters by numerical simulation is provided,which is significantly advantageous over that of the grating bonding FBG.The theoretical equation is verified by finite element method(FEM).Compared with the existing model,the proposed model has higher measurement accuracy.Experimental tests are performed to validate the effectiveness of the proposed model on the equalintensity cantilever beam,whose surface is attached to the bare FBG with grating ends bonding and strain gauge by using epoxy glue.The results show that there is a great agreement between the outcome of the bare FBG and that of the strain gauge,and the corrected strain is closer to the true strain.The proposed model provides a theoretical basis for the design of the grating ends surface-bonded FBG strain sensor for health monitoring of large structures.展开更多
Aquilaria si,iensis, a kind of typically wounding-induced medicinal plant with a great economical value, is widely used in the production of traditional Chinese medicine, perfume and incense. Coronatine-insensitive pr...Aquilaria si,iensis, a kind of typically wounding-induced medicinal plant with a great economical value, is widely used in the production of traditional Chinese medicine, perfume and incense. Coronatine-insensitive protein 1 (COI1) acts as a receptor in jasmonate (JA) signaling pathway, and regulates the expression of JA-responsive genes in plant defense. However, little is known about the COI1 gene in A, sinensis, Here, based on the transcriptome data a full-length cDNA sequence of COI1 (termed as AsCOM was firstly cloned by RT PCR and rapid amplification of cDNA ends (RACE) strategies. AsCOI1 is 2330 bp in length (GenBank accession No. KM 189194), and contains a complete open frame (ORF) of 1839 bp. The deduced protein was composed of 612 amino acids, with a predicted molecular weight of 68.93 kDa and an iisoelectric point of 6.56, and was predicted to possess b-box and ERRs domains. Combining bioinformatics prediction with subcellular localization experiment analysis, AsCOI1 was appeared to locate in nucleus. AsCOI1 gene was highly expressed in roots and stems, the major organs of agarvimod formation. Methyl jasmonate (MeJA), mechanical wounding and heat stress could significantly induce the expression level of AsCOI1 gene. AsCOI1 is an early wound -responsive gene, and it likely plays sonic role in agarwood formation. (c) 2015 Chinese Pharmaceutical Association and Institute of Materia Medica Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.展开更多
To investigate the molecular basis of porcine heterosis, suppression subtractive hybridization （SSH） was performed to detect the differences in gene expression between porcine longissimus dorsi of Meishan X Large Wh...To investigate the molecular basis of porcine heterosis, suppression subtractive hybridization （SSH） was performed to detect the differences in gene expression between porcine longissimus dorsi of Meishan X Large White （MS × LW） F1 hybrids and their parents Meishan pigs. An expression sequence tag （EST） differentially expressed was found, designated as ML556, which was homologous to a hypothetical protein HSPC117, from human hematopoietic stem/progenitor cells （HSPCs）, and the full-length cDNA of porcine HSPC117 was cloned using rapid amplification of cDNA ends （RACE） method. Translation of the mRNA transcript revealed an open reading frame （ORF） of 505 amino acid residues encoding a peroxisomal targeting signal （PTS） with theoretical molecular weight of 55 kDa. Alignment analysis revealed that the deduced protein sequence exhibit 98, 98, 98, 97, and 97% identity with that of cattle, human, dog, rat, and mouse, respectively. The tissue expression analysis indicated that the porcine HSPC117 gene is highly expressed in muscle, spleen, lung, kidney, uterus, ovary and testis, moderately expressed in fat, heart, and liver, and not expressed in stomach and small intestine. The possible role of porcine HSPC117 and its relationship with porcine heterosis were discussed.展开更多
Gateways at faces of great mining heights are mostly driven along the roof of coal seams.For gateway height restrictions,a 1-3 m floor coal is retained,leaving a triangular floor coal at the face ends,causing a loss o...Gateways at faces of great mining heights are mostly driven along the roof of coal seams.For gateway height restrictions,a 1-3 m floor coal is retained,leaving a triangular floor coal at the face ends,causing a loss of coal.In order to improve coal recovery rates and to ensure efficiency of equipment at coal mining faces,we investigated suitable retention methods and recovery technology of floor coal at face ends.The upper floor coal can directly be recovered by a shearer with floor dinting.The lower floor coal is recovered by shearer with floor dinting after advanced floor dinting and retaining a step for protecting coal sides in a haulage gateway.Field practice shows that this method can improve the coal recovery rates at fully mechanized working faces with great mining heights.展开更多
In this work we report that after single-walled carbon nanotubes(SWNTs) are sheared with a pair of titanium scissors,the magnetization becomes larger than that of the corresponding pristine ones. The magnetization inc...In this work we report that after single-walled carbon nanotubes(SWNTs) are sheared with a pair of titanium scissors,the magnetization becomes larger than that of the corresponding pristine ones. The magnetization increases proportionally with the number of SWNTs with sheared ends, suggesting that there exist magnetic moments at the sheared ends of SWNTs.By using the coefficient of this linear relation, the average magnetic moment is estimated to be 41.5 ± 9.8 μB(Bohr magneton) per carbon atom in the edge state at temperature of 300.0 K, suggesting that ultrahigh magnetic fields can be produced. The dangling sigma and pi bonds of the carbon atoms at sheared ends play important roles in determining the unexpectedly high magnetic moments, which may have great potential applications.展开更多
基金supported by National Natural Science Foundation for Distinguished Young Scholar of China (Grant No. 50925518)National Natural Science Foundation of China (Grant No. 50775226)+1 种基金Key Project of Ministry of Education of China(Grant No. 109129)Chongqing Municipal Key Scientific and Technological Project of China (Grant No. CSTC2009AC3049)
文摘A stranded wires helical spring is formed of a multilayer and coaxial strand of several wires twisted together with the same direction of spiral. Compared with the conventional single wire spring, the stranded wires helical spring has the notable predominance in strength, damping and vibration reduction, which is usually used in aircraft engines, automatic weapons, etc. However, due to its complicated structure, the precise computation of its strength and rigidity need be a correct mathematical model, which then will be imported to finite element analysis software for solutions. Equations on solving geometric parameters, such as external diameters of strands and screw pitches of wires, are put forward in the paper. It also proposes a novel methodology on solving geometric parameters and establishing entity models of the stranded wires helical spring, which provides foundation of computing mechanical parameters by FEA. Then mathematical models on the centre line of the strand and the surface curve of each wire, after closing two ends in a spring, are proposed. Finally, geometric parameters are solved in a case study, and a 3D entity model of a spring with 3 layers and 16 wires is established, which has validated the accuracy of the proposed methodology and the 3D entity mathematical model. The method provides a new way to design stranded wire helical spring.
文摘Blue-grained wheat derived from the hybrid Triticum aestivum L. X Thinopyrum ponticum (Podp.) Barkworth et D. R. Dewey (Agropyron elongatum (Host) P. Beauv., 2n=70). The molecular biological mechanism of the biosynthetic pathway of blue pigments in the blue grain remains unclear yet. Dihydroflavonol 4-reductase (DFR) is one of the key enzymes controlling flavonoid synthesis in anthocyanin biosynthetic pathway, and may directly participate in the formation of blue pigment in the aleurone layer of blue-grained wheat. Here we cloned a DFR cDNA (TaDFR) from the developing seeds of blue-grained wheat, and four DFR genomic DNAs from Th. ponticum (ThpDFR.t), blue-grained wheat (TaDFR.bg), white-grained offspring of light blue-grained wheat (TaDFR.wg) and Chinese Spring (2n=42) (TaDFR.csg), respectively. TaDFR cDNA encodes a 354 amino-acids polypeptide with high identity to DFR from Hordeum vulgare L. (94%), Oryza sativa L. (83%), Zea mays L.(84%). The result of cluster analysis showed that TaDFR cDNA nucleotide sequence has 100% identity with that of TaDFR.csg. The four DFR genomic DNAs have extraordinary high homology and each has three introns. The differences of the four DFR genomic DNAs mainly exist in introns. Southern blotting analysis showed that there are at least 3-5 DFR copies in wheat, the copy numbers in different color grain wheats are not significantly different. The hybridization band patterns were the same, but different from that of Th. ponticum. DFR in blue-grained wheat belongs to a DFR superfamily. Northern blotting analysis indicated that the DFR expressed in the developing seeds of both blue- and white-grained wheat at 15 d after flowering (DAF), the mRNA levels of DFR reached the highest at 18 DAF, then declined quickly and disappeared at 33 DAF But the expression levels in blue-grained seeds were higher than that in white grain at the same seed developing stages. DFR transcripts accumulated in young leaves, and leaf sheaths of blue- and white-grained wheat and Th ponticum, but not detected in roo
基金Supported by the Hi-Tech Research and Development(863) Program of China,and the National Natural Science Foundation of China(30500303)
文摘Farnesyl dlphosphate synthase （FPS; EC 2.5.1.10） catalyzes the production of 15-carbon farnesyl dlphosphate which Is a branch-point Intermediate for many terpenoids. This reaction Is considered to be a ratelimiting step In terpenold biosynthesis. Here we report for the first time the cloning of a new full-length cDNA encoding farnesyl dlphosphate synthase from a gymnosperm plant species, Taxus media Rehder, designated as TmFPS1. The full-length cDNA of TmFPS1 （GenBank accession number： AY461811） was 1 464 bp with a 1 056-bp open reading frame encoding a 351-amino acid polypeptlde with a calculated molecular weight of 40.3 kDa and a theoretical pl of 5.07. Biolnformatlc analysis revealed that TmFPS1 contained all five conserved domains of prenyltransferases, and showed homology to other FPSs of plant origin. Phylogenetlc analysis showed that farnesyl dlphosphate synthases can be divided Into two groups： one of prokaryotic origin and the other of eukaryotic origin. TmFPS1 was grouped with FPSs of plant origin. Homologybased structural modeling showed that TmFPS1 had the typical spatial structure of FPS, whose most prominent structural feature Is the arrangement of 13 core helices around a large central cavity In which the catalytic reaction takes place. Our blolnformatic analysis strongly suggests that TmFPS1 is a functional gene. Southern blot analysis revealed that TmFPS1 belongs to a small FPSgene family in T. media. Northern blot analysis indicated that TmFPS1 is expressed in all tested tissues, Including the needles, stems and roots of T. media. Subsequently, functional complementatlon with TmFPS1 in a FPS-deflclent mutant yeast demonstrated that TmFPS1 did encode farnesyl dlphosphate synthase, which rescued the yeast mutant. This study will be helpful In future Investigations aiming at understanding the detailed role of FPS In terpenold biosynthesis flux control at the molecular genetic level.
文摘The grating ends bonding fiber Bragg grating(FBG)sensor has been widely used in sensor packages such as substrate type and clamp type for health monitoring of large structures.However,owing to the shear deformation of the adhesive layer of FBG,the strain measured by FBG is often different from the strain of actual matrix,which causes strain measurement errors.This investigation aims at improving the measurement accuracy of strain for the grating ends surface-bonded FBG.To fulfill this objective,a strain transfer equation of the grating ends bonding FBG is derived,and a theoretical model of the average strain transfer from the matrix to the optical fiber is developed.Moreover,parameters that influence the average strain transfer rate from the matrix to the optical fiber are analyzed.A selection scheme of bonding parameters by numerical simulation is provided,which is significantly advantageous over that of the grating bonding FBG.The theoretical equation is verified by finite element method(FEM).Compared with the existing model,the proposed model has higher measurement accuracy.Experimental tests are performed to validate the effectiveness of the proposed model on the equalintensity cantilever beam,whose surface is attached to the bare FBG with grating ends bonding and strain gauge by using epoxy glue.The results show that there is a great agreement between the outcome of the bare FBG and that of the strain gauge,and the corrected strain is closer to the true strain.The proposed model provides a theoretical basis for the design of the grating ends surface-bonded FBG strain sensor for health monitoring of large structures.
基金supported by grants from the National Natural Science Foundation of China (Nos. 31100220, 81173481 and 31000136)the Program for Xiehe Scholars in Chinese Academy of Medical Sciences & Peking Union Medical College (No. 282)the Innovative Team and Innovative Talents Project of the Ministry of Science and Technology of China
文摘Aquilaria si,iensis, a kind of typically wounding-induced medicinal plant with a great economical value, is widely used in the production of traditional Chinese medicine, perfume and incense. Coronatine-insensitive protein 1 (COI1) acts as a receptor in jasmonate (JA) signaling pathway, and regulates the expression of JA-responsive genes in plant defense. However, little is known about the COI1 gene in A, sinensis, Here, based on the transcriptome data a full-length cDNA sequence of COI1 (termed as AsCOM was firstly cloned by RT PCR and rapid amplification of cDNA ends (RACE) strategies. AsCOI1 is 2330 bp in length (GenBank accession No. KM 189194), and contains a complete open frame (ORF) of 1839 bp. The deduced protein was composed of 612 amino acids, with a predicted molecular weight of 68.93 kDa and an iisoelectric point of 6.56, and was predicted to possess b-box and ERRs domains. Combining bioinformatics prediction with subcellular localization experiment analysis, AsCOI1 was appeared to locate in nucleus. AsCOI1 gene was highly expressed in roots and stems, the major organs of agarvimod formation. Methyl jasmonate (MeJA), mechanical wounding and heat stress could significantly induce the expression level of AsCOI1 gene. AsCOI1 is an early wound -responsive gene, and it likely plays sonic role in agarwood formation. (c) 2015 Chinese Pharmaceutical Association and Institute of Materia Medica Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.
基金financially supported by National Basic Research Program of China(973 Program,2006CB102102)National Natural Science Foundation of China(30400313).
文摘To investigate the molecular basis of porcine heterosis, suppression subtractive hybridization （SSH） was performed to detect the differences in gene expression between porcine longissimus dorsi of Meishan X Large White （MS × LW） F1 hybrids and their parents Meishan pigs. An expression sequence tag （EST） differentially expressed was found, designated as ML556, which was homologous to a hypothetical protein HSPC117, from human hematopoietic stem/progenitor cells （HSPCs）, and the full-length cDNA of porcine HSPC117 was cloned using rapid amplification of cDNA ends （RACE） method. Translation of the mRNA transcript revealed an open reading frame （ORF） of 505 amino acid residues encoding a peroxisomal targeting signal （PTS） with theoretical molecular weight of 55 kDa. Alignment analysis revealed that the deduced protein sequence exhibit 98, 98, 98, 97, and 97% identity with that of cattle, human, dog, rat, and mouse, respectively. The tissue expression analysis indicated that the porcine HSPC117 gene is highly expressed in muscle, spleen, lung, kidney, uterus, ovary and testis, moderately expressed in fat, heart, and liver, and not expressed in stomach and small intestine. The possible role of porcine HSPC117 and its relationship with porcine heterosis were discussed.
基金the Independent Research of the State Key Laboratory of Coal Resources and Mine Safety(No. SKLCRSM09X02)the Open Research Fund of the State Key Laboratory of Coal Resources and Mine Safety(No.08KF12)the Graduate Students of Jiangsu Province Innovation Program Funded Projects(No.CX09B_120Z) for their financial support
文摘Gateways at faces of great mining heights are mostly driven along the roof of coal seams.For gateway height restrictions,a 1-3 m floor coal is retained,leaving a triangular floor coal at the face ends,causing a loss of coal.In order to improve coal recovery rates and to ensure efficiency of equipment at coal mining faces,we investigated suitable retention methods and recovery technology of floor coal at face ends.The upper floor coal can directly be recovered by a shearer with floor dinting.The lower floor coal is recovered by shearer with floor dinting after advanced floor dinting and retaining a step for protecting coal sides in a haulage gateway.Field practice shows that this method can improve the coal recovery rates at fully mechanized working faces with great mining heights.
基金Project supported by the National Key Research and Development Program of China(Grant Nos.2018YFA0208403 and 2016YFA0200403)the National Natural Science Foundation of China(Grant Nos.51472057,11874129,91323304,and 11674387)+1 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDA09040101)the Baotou Rare Earth Research and Development Centre,Chinese Academy of Sciences(Grant No.GZR 2018001)
文摘In this work we report that after single-walled carbon nanotubes(SWNTs) are sheared with a pair of titanium scissors,the magnetization becomes larger than that of the corresponding pristine ones. The magnetization increases proportionally with the number of SWNTs with sheared ends, suggesting that there exist magnetic moments at the sheared ends of SWNTs.By using the coefficient of this linear relation, the average magnetic moment is estimated to be 41.5 ± 9.8 μB(Bohr magneton) per carbon atom in the edge state at temperature of 300.0 K, suggesting that ultrahigh magnetic fields can be produced. The dangling sigma and pi bonds of the carbon atoms at sheared ends play important roles in determining the unexpectedly high magnetic moments, which may have great potential applications.
