As the source of energy and biological signals,light can influence the healing process of grafted seedlings by regulating the synthesis of the endogenous hormone,regeneration of wound-healing tissue,and connection of ...As the source of energy and biological signals,light can influence the healing process of grafted seedlings by regulating the synthesis of the endogenous hormone,regeneration of wound-healing tissue,and connection of vascular tissue in grafted seedlings.The effect of daily light integral(DLI)on the healing process and seedling quality of tomato(Solanum lycopersicum L.)was analyzed in this study,with the comparison of grafted seedlings treated in dark for 7 d after grafting.The results showed that the height increment of scion and rootstock,adhesion of graft union,stem flow,total chlorophyll content,and net photosynthesis rate increased gradually with increasing light intensity,and no longer increased significantly when the DLI was higher than 5.04 mol/m^(2)·d.The contents of auxin(IAA)and gibberellin(GA)in tomato leaves increased and abscisic acid(ABA)decreased with the increase of DLI.However,there was no significant difference between the treatments with DLI higher than 6.48 mol/m^(2)·d.Both the biomass and energy use efficiency(EUE)of grafted seedlings increased with DLI in a certain range and then decreased.The biomass was the largest when DLI was 5.04 mol/m^(2)·d.However,EUE was highest when DLI was 7.46 mol/m^(2)·d.In conclusion,a suitable DLI is beneficial to cultivate high-quality grafted tomato seedlings,and increasing DLI within a certain range can promote biomass accumulation,connection of vascular tissue,and endogenous hormone biosynthesis in tomato grafted seedlings during the healing period.The lighting environment with DLI of 5.04 mol/m^(2)·d(light intensity of 100μmol/m^(2)·s and light time of 14 h/d)is recommended for the healing treatment in high-quality production,which also improves EUE during the healing period of tomato grafted seedlings.展开更多
Objectives: Fluorescence spectroscopy which can be used for optical tissue diagnosis of tumor pathology?deserves special interest. The purpose of the work was to study blood plasma and tumor tissue of men with differe...Objectives: Fluorescence spectroscopy which can be used for optical tissue diagnosis of tumor pathology?deserves special interest. The purpose of the work was to study blood plasma and tumor tissue of men with different forms of prostate tumors by using laser induced fluorescence. Blood plasma and tumor tissue of the patients with benign hyperplasia of the prostate (BHP), BHP with inflammation, BHP with high grade PIN (BHP with HGPIN) and adenocarcinoma of prostate (CaP) have been studied. Results: In case of blood plasma fluorescence,?intensity of the plasma proteins corresponding peak (340 - 360 nm) was increasing in the following manner: control group → BHP → BHP with HGPIN → CaP. The intensity of the nicotinamide coenzymes correspond peak (440 - 460 nm) was increased in case of BHP with HGPIN and CaP patients, but decreased in case of BHP, compared to control. In case of tumor tissue, the changes of the collagen peak (390 - 400 nm) intensity have been revealed in all cases of prostate tumor tissues. These alterations point to altered collagen biosynthesis levels in different tumor tissues, that reflects the structural changes and characteristics of malignant transformation. Also the changes of the nicotinamide coenzymes peak (440 - 460 nm) intensity in all spectra of tumor tissues were observed. The highest intensity of the peak was observed in the spectra of BHP with HGPIN and in prostate cancer tissue. Conclusions: Alterations of the coenzymes peak intensities perfectly reflect and are in accordance with the specific energy metabolism of prostate epithelial cells. Normalization of fluorescent spectra from different forms of prostate tumor tissues has shown that, each form has typical spectral shape and ratio of fluorescence peaks intensities.展开更多
基金supported by Postdoctoral Fund in Jiangsu Province(2020Z308)Project Funded by the National Key Research and Development Program of China(2018YFF0213601)+1 种基金Project Funded by the Key Laboratory of Modern Agricultural Equipment and Technology of the Ministry of Education(JNZ201909)Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD-2018-87).
文摘As the source of energy and biological signals,light can influence the healing process of grafted seedlings by regulating the synthesis of the endogenous hormone,regeneration of wound-healing tissue,and connection of vascular tissue in grafted seedlings.The effect of daily light integral(DLI)on the healing process and seedling quality of tomato(Solanum lycopersicum L.)was analyzed in this study,with the comparison of grafted seedlings treated in dark for 7 d after grafting.The results showed that the height increment of scion and rootstock,adhesion of graft union,stem flow,total chlorophyll content,and net photosynthesis rate increased gradually with increasing light intensity,and no longer increased significantly when the DLI was higher than 5.04 mol/m^(2)·d.The contents of auxin(IAA)and gibberellin(GA)in tomato leaves increased and abscisic acid(ABA)decreased with the increase of DLI.However,there was no significant difference between the treatments with DLI higher than 6.48 mol/m^(2)·d.Both the biomass and energy use efficiency(EUE)of grafted seedlings increased with DLI in a certain range and then decreased.The biomass was the largest when DLI was 5.04 mol/m^(2)·d.However,EUE was highest when DLI was 7.46 mol/m^(2)·d.In conclusion,a suitable DLI is beneficial to cultivate high-quality grafted tomato seedlings,and increasing DLI within a certain range can promote biomass accumulation,connection of vascular tissue,and endogenous hormone biosynthesis in tomato grafted seedlings during the healing period.The lighting environment with DLI of 5.04 mol/m^(2)·d(light intensity of 100μmol/m^(2)·s and light time of 14 h/d)is recommended for the healing treatment in high-quality production,which also improves EUE during the healing period of tomato grafted seedlings.
文摘Objectives: Fluorescence spectroscopy which can be used for optical tissue diagnosis of tumor pathology?deserves special interest. The purpose of the work was to study blood plasma and tumor tissue of men with different forms of prostate tumors by using laser induced fluorescence. Blood plasma and tumor tissue of the patients with benign hyperplasia of the prostate (BHP), BHP with inflammation, BHP with high grade PIN (BHP with HGPIN) and adenocarcinoma of prostate (CaP) have been studied. Results: In case of blood plasma fluorescence,?intensity of the plasma proteins corresponding peak (340 - 360 nm) was increasing in the following manner: control group → BHP → BHP with HGPIN → CaP. The intensity of the nicotinamide coenzymes correspond peak (440 - 460 nm) was increased in case of BHP with HGPIN and CaP patients, but decreased in case of BHP, compared to control. In case of tumor tissue, the changes of the collagen peak (390 - 400 nm) intensity have been revealed in all cases of prostate tumor tissues. These alterations point to altered collagen biosynthesis levels in different tumor tissues, that reflects the structural changes and characteristics of malignant transformation. Also the changes of the nicotinamide coenzymes peak (440 - 460 nm) intensity in all spectra of tumor tissues were observed. The highest intensity of the peak was observed in the spectra of BHP with HGPIN and in prostate cancer tissue. Conclusions: Alterations of the coenzymes peak intensities perfectly reflect and are in accordance with the specific energy metabolism of prostate epithelial cells. Normalization of fluorescent spectra from different forms of prostate tumor tissues has shown that, each form has typical spectral shape and ratio of fluorescence peaks intensities.
