Aqueous roots extract of Alchornea cordifolia was studied to evaluate its traditional antifertility claim. Antifertility effect of Alchornea cordifolia was evaluated according to the stages of pregnancy of female rats...Aqueous roots extract of Alchornea cordifolia was studied to evaluate its traditional antifertility claim. Antifertility effect of Alchornea cordifolia was evaluated according to the stages of pregnancy of female rats. In the first stage, forty female rats were randomly divided into four groups (10 rats/group) and respectively treated with 1 ml distilled water (control), 100, 200 and 400 mg/kg body weight (BW) per day of Alchornea cordifolia. After laparotomy and at parturition, sites of implantation and resorption on the uterine horns were determined. The ovaries and uterus were collected and weighed. Blood samples were collected for hormonal assay. In the second and third stage of pregnancy, treatment schedule and antifertility parameters were assessed as above for the first stage. Treated groups received daily 400 mg/kg bw of extract of Alchornea cordifolia while Control group received distilled water. It was observed in the first stage of pregnancy that, the number of resorption sites significantly (p < 0.05) decreased at 100 and 200 mg/kg. No implantation site was observed at dose 400 mg/kg after laparotomy as well as the number of pups at parturition at all doses. The body weight of ovaries and uterine horns at dose 400 mg/kg significantly (p < 0.05) decreased when compared to the control. The extract dose dependently decreased significantly the concentrations of serum progesterone. In the second stage of pregnancy after laparotomy and at parturition, the number of live fetus and the survival ratio significantly (p < 0.001) decreased and were associated with the vaginal discharge and bleeding of animals. In the third stage of pregnancy, the extract caused an early parturition associated with a decreased number of live fetus and the survival ratio with p The results indicate that aqueous roots extract of Alchornea cordifolia could induce inhibitory effects on reproductive functions in female albino rats.展开更多
Objective:To investigate the influence of serum E2 level on pregnancy outcome on endometrial transformation day in hormone replacement therapy-thawed embryo transfer(HRT-FET)cycle.Methods:Totally 834 embryo transfer c...Objective:To investigate the influence of serum E2 level on pregnancy outcome on endometrial transformation day in hormone replacement therapy-thawed embryo transfer(HRT-FET)cycle.Methods:Totally 834 embryo transfer cycles of cleavage stage treated by HRT-FET in Reproductive Center of First Affiliated Hospital of Hainan Medical College from December 2014 to December 2019 were analyzed retrospectively.Results:The number of transplanted embryos in group A was higher than that in group B,and the difference was statistically significant(P<0.05);The thickness and serum P level of endometrial transformation day in Group B were significantly higher than those in Group A(P<0.05);There was no significant difference in clinical pregnancy rate,implantation rate,abortion rate,ectopic pregnancy rate,biochemical pregnancy rate and live birth rate between Group A and Group B(P>0.05);There was no statistical difference in general data between group A1 and group A2,group B1 and group B2(P>0.05);There was no significant difference in clinical pregnancy rate,implantation rate,abortion rate,ectopic pregnancy rate,biochemical pregnancy rate and live birth rate between group B1 and group B2(P>0.05);The clinical pregnancy rate,implantation rate and live birth rate in group A2 were significantly higher than those in group A1(P<0.05).Conclusions:Endometrial type A or B has no significant effect on pregnancy outcome in hormone replacement therapy-thawed embryo transfer cycle.;In type A endometrium,the serum E2>827 pmol/L on the day of endometrial transformation is helpful to improve the pregnancy outcome of frozen-thawed embryo transfer.展开更多
文摘Aqueous roots extract of Alchornea cordifolia was studied to evaluate its traditional antifertility claim. Antifertility effect of Alchornea cordifolia was evaluated according to the stages of pregnancy of female rats. In the first stage, forty female rats were randomly divided into four groups (10 rats/group) and respectively treated with 1 ml distilled water (control), 100, 200 and 400 mg/kg body weight (BW) per day of Alchornea cordifolia. After laparotomy and at parturition, sites of implantation and resorption on the uterine horns were determined. The ovaries and uterus were collected and weighed. Blood samples were collected for hormonal assay. In the second and third stage of pregnancy, treatment schedule and antifertility parameters were assessed as above for the first stage. Treated groups received daily 400 mg/kg bw of extract of Alchornea cordifolia while Control group received distilled water. It was observed in the first stage of pregnancy that, the number of resorption sites significantly (p < 0.05) decreased at 100 and 200 mg/kg. No implantation site was observed at dose 400 mg/kg after laparotomy as well as the number of pups at parturition at all doses. The body weight of ovaries and uterine horns at dose 400 mg/kg significantly (p < 0.05) decreased when compared to the control. The extract dose dependently decreased significantly the concentrations of serum progesterone. In the second stage of pregnancy after laparotomy and at parturition, the number of live fetus and the survival ratio significantly (p < 0.001) decreased and were associated with the vaginal discharge and bleeding of animals. In the third stage of pregnancy, the extract caused an early parturition associated with a decreased number of live fetus and the survival ratio with p The results indicate that aqueous roots extract of Alchornea cordifolia could induce inhibitory effects on reproductive functions in female albino rats.
基金This study was supported by 2021 Hainan Science and Technology Plan Project(Hainan Clinical Medical Research Center)(No.LCYX202102)。
文摘Objective:To investigate the influence of serum E2 level on pregnancy outcome on endometrial transformation day in hormone replacement therapy-thawed embryo transfer(HRT-FET)cycle.Methods:Totally 834 embryo transfer cycles of cleavage stage treated by HRT-FET in Reproductive Center of First Affiliated Hospital of Hainan Medical College from December 2014 to December 2019 were analyzed retrospectively.Results:The number of transplanted embryos in group A was higher than that in group B,and the difference was statistically significant(P<0.05);The thickness and serum P level of endometrial transformation day in Group B were significantly higher than those in Group A(P<0.05);There was no significant difference in clinical pregnancy rate,implantation rate,abortion rate,ectopic pregnancy rate,biochemical pregnancy rate and live birth rate between Group A and Group B(P>0.05);There was no statistical difference in general data between group A1 and group A2,group B1 and group B2(P>0.05);There was no significant difference in clinical pregnancy rate,implantation rate,abortion rate,ectopic pregnancy rate,biochemical pregnancy rate and live birth rate between group B1 and group B2(P>0.05);The clinical pregnancy rate,implantation rate and live birth rate in group A2 were significantly higher than those in group A1(P<0.05).Conclusions:Endometrial type A or B has no significant effect on pregnancy outcome in hormone replacement therapy-thawed embryo transfer cycle.;In type A endometrium,the serum E2>827 pmol/L on the day of endometrial transformation is helpful to improve the pregnancy outcome of frozen-thawed embryo transfer.
