Objective:To investigate the larvicidal activity of synthesized silver nanoparticles（Ag NPs） utilizing aqueous bark extract of Ficus racemosa（F.racemosa） was tested against fourth instar larvae of filariasis vecto...Objective:To investigate the larvicidal activity of synthesized silver nanoparticles（Ag NPs） utilizing aqueous bark extract of Ficus racemosa（F.racemosa） was tested against fourth instar larvae of filariasis vector,Culex quinquefasciatus（Cx.quinquefasciatus） and japanese encephalitis vectors,Culex gelidus（Cx.gelidus）.Methods:The synthesized Ag NPs was characterized by UV-vis spectrum,X-ray diffraction（XRD）,Scanning electron microscopy（SEM） and Fourier transform infrared（FTIR）.The larvicidal activities were assessed for 24 h against the larvae of Cx.quinquefasciatus and Cx.gelidus with varying concentrations of aqueous bark extract off.racemosa and synthesized Ag NPs.LC<sub>50</sub> and r<sup>2</sup> values were calculated.Results:The maximum efficacy was observed in crude aqueous extract of F.racemosa against the larvae of Cx.quinquefasciatus and Cx.gelidus(LC<sub>50</sub>=67.72 and 63.70 mg/L;r<sup>2</sup>=0.995 and 0.985) and the synthesized Ag NPs(LC<sub>50</sub>=12.00 and 11.21 mg/L;r<sup>2</sup>=0.997 and 0.990).respectively.Synthesized Ag NPs showed the XRD peaks at 2θvalues of 27.61,29.60,35.48,43.48 and 79.68 were identified as （210）,（121）,（220）,（200） and（311） reflections,respectively.The FTIR spectra of Ag NPs exhibited prominent peaks at 3 425,2 878,1 627 and 1 382 in the region 500-3 000 cm<sup>-1</sup>.The peaks correspond to the presence of a stretching vibration of（NH） C=O group.SEM analysis showed shape in cylindrical,uniform and rod with the average size of 250.60 nm.Conclusions:The biosynthesis of silver nanoparticles using bark aqueous extract of F.racemosa and its larvicidal activity against the larvae of disease spreading vectors.The maximum larvicidal efficacy was observed in the synthesized Ag NPs.展开更多
Objective:To assess the acaricidal activity of titanium dioxide nanoparticles(TiO_2 NPs)synthesized from flower aqueous extract of Calotropis gigantea(C.gigantea)against the larvae of Rhipicephalus(Boophilus)microplus...Objective:To assess the acaricidal activity of titanium dioxide nanoparticles(TiO_2 NPs)synthesized from flower aqueous extract of Calotropis gigantea(C.gigantea)against the larvae of Rhipicephalus(Boophilus)microplus[R.(B.)microplus]and the adult of Haemaphrysalis bispinosa(H.bispinosa).Methods:The lyophilized C.gigantea flower aqueous extract of 50 mg was added with 100 mL of TiO(OH_2)(10 mM)and magnetically stirred for 6 h.Synthesized TiO_2 NPs were characterized by X-ray diffraction(XRD).Fourier transform infrared spectroscopy(FTIR),Scanning electron microscopy(SEM),and Energy dispersive X-ray spectroscopy(EDX).The synthesised TiO_2 NPs were tested against the larvae of R(B.)microplus and adult of H.bispinosa were exposed to filter paper impregnated method.Results:XRD confirmed the crystalline nature of the nanoparticles with the mean size of 10.52 nm.The functional groups for synthesized TiO_2NPs were 1405.19,and 1053.45 cm^(-1)for-NH_2 bending,primary amines and amides and 1053.84and 1078.45 cm^(-1)for C-O.SEM micrographs of the synthesized TiO_2 NPs showed the aggregated and spherical in shape.The maximum efficacy was observed in the aqueous flower extract of C.gigantea and synthesized TiO_2 NPs against R.(B.)microplus(LC_(50)=24.63 and 5.43 mg/L and r^2=0.960 and 0.988)and against H.bispinosa(LC_(50)=35.22 and 9.15 mg/L and r^2=0.969 and 0.969).respectively.Conclusions:The synthesized TiO_2 NPs were highly stable and had significant acaricidal activity against the larvae of R.(B.)microplus and adult of H.bispinosa.This study provides the first report of synthesized TiO_2 NPs and possessed excellent anti-parasitic activity.展开更多
A diplomonad flagellate Hexamita capsularis sp.nov.from the posterior intestine of the fish(Xenocypris dividi)from Liangzi Lake,Hubei Province,is described.The flagellate was oval,withsmooth surface and homogenous cyt...A diplomonad flagellate Hexamita capsularis sp.nov.from the posterior intestine of the fish(Xenocypris dividi)from Liangzi Lake,Hubei Province,is described.The flagellate was oval,withsmooth surface and homogenous cytoplasm,6.21±0.28μm in length,3.43±0.48μm in width and with anelliptical capsule.Two recurrent flagellae arranged between two nuclei ran longitudinally to the posteriorend.Fibrillar bands connected the accessory organelles of two cytostomal tubes,the body depressed atthe posterior end of the body.展开更多
The mechanical properties of several kinds of coal gangue calcined with limestone were Researched so as to find the optimum way of calcinations with limestone. Microstructure and property of hydration process of cemen...The mechanical properties of several kinds of coal gangue calcined with limestone were Researched so as to find the optimum way of calcinations with limestone. Microstructure and property of hydration process of cement pastes containing added-calcium coal gangue were analyzed by means of scanning electron microscope （SEM） and method of mercury in trusion poremeasurement （MIP）, etc. The experiment can approve those results： when proper amounst of gypsum and fluorite were taken as mineralizers in the course of calcinations of added-calcium coal gangue, activity of coal gangue can be effectively improved. The results of mechanical property and structural characteristic such as hydration process, hydration product and microstructure etc. of cement pastes containing added-calcium coal gangue are consistent.展开更多
文摘Objective:To investigate the larvicidal activity of synthesized silver nanoparticles（Ag NPs） utilizing aqueous bark extract of Ficus racemosa（F.racemosa） was tested against fourth instar larvae of filariasis vector,Culex quinquefasciatus（Cx.quinquefasciatus） and japanese encephalitis vectors,Culex gelidus（Cx.gelidus）.Methods:The synthesized Ag NPs was characterized by UV-vis spectrum,X-ray diffraction（XRD）,Scanning electron microscopy（SEM） and Fourier transform infrared（FTIR）.The larvicidal activities were assessed for 24 h against the larvae of Cx.quinquefasciatus and Cx.gelidus with varying concentrations of aqueous bark extract off.racemosa and synthesized Ag NPs.LC<sub>50</sub> and r<sup>2</sup> values were calculated.Results:The maximum efficacy was observed in crude aqueous extract of F.racemosa against the larvae of Cx.quinquefasciatus and Cx.gelidus(LC<sub>50</sub>=67.72 and 63.70 mg/L;r<sup>2</sup>=0.995 and 0.985) and the synthesized Ag NPs(LC<sub>50</sub>=12.00 and 11.21 mg/L;r<sup>2</sup>=0.997 and 0.990).respectively.Synthesized Ag NPs showed the XRD peaks at 2θvalues of 27.61,29.60,35.48,43.48 and 79.68 were identified as （210）,（121）,（220）,（200） and（311） reflections,respectively.The FTIR spectra of Ag NPs exhibited prominent peaks at 3 425,2 878,1 627 and 1 382 in the region 500-3 000 cm<sup>-1</sup>.The peaks correspond to the presence of a stretching vibration of（NH） C=O group.SEM analysis showed shape in cylindrical,uniform and rod with the average size of 250.60 nm.Conclusions:The biosynthesis of silver nanoparticles using bark aqueous extract of F.racemosa and its larvicidal activity against the larvae of disease spreading vectors.The maximum larvicidal efficacy was observed in the synthesized Ag NPs.
文摘Objective:To assess the acaricidal activity of titanium dioxide nanoparticles(TiO_2 NPs)synthesized from flower aqueous extract of Calotropis gigantea(C.gigantea)against the larvae of Rhipicephalus(Boophilus)microplus[R.(B.)microplus]and the adult of Haemaphrysalis bispinosa(H.bispinosa).Methods:The lyophilized C.gigantea flower aqueous extract of 50 mg was added with 100 mL of TiO(OH_2)(10 mM)and magnetically stirred for 6 h.Synthesized TiO_2 NPs were characterized by X-ray diffraction(XRD).Fourier transform infrared spectroscopy(FTIR),Scanning electron microscopy(SEM),and Energy dispersive X-ray spectroscopy(EDX).The synthesised TiO_2 NPs were tested against the larvae of R(B.)microplus and adult of H.bispinosa were exposed to filter paper impregnated method.Results:XRD confirmed the crystalline nature of the nanoparticles with the mean size of 10.52 nm.The functional groups for synthesized TiO_2NPs were 1405.19,and 1053.45 cm^(-1)for-NH_2 bending,primary amines and amides and 1053.84and 1078.45 cm^(-1)for C-O.SEM micrographs of the synthesized TiO_2 NPs showed the aggregated and spherical in shape.The maximum efficacy was observed in the aqueous flower extract of C.gigantea and synthesized TiO_2 NPs against R.(B.)microplus(LC_(50)=24.63 and 5.43 mg/L and r^2=0.960 and 0.988)and against H.bispinosa(LC_(50)=35.22 and 9.15 mg/L and r^2=0.969 and 0.969).respectively.Conclusions:The synthesized TiO_2 NPs were highly stable and had significant acaricidal activity against the larvae of R.(B.)microplus and adult of H.bispinosa.This study provides the first report of synthesized TiO_2 NPs and possessed excellent anti-parasitic activity.
基金This work is a part of the M.S.thesis of Xiao Wu-han and was supported by the National Natural Science Foundation of China (Grant NO.3870489).
文摘A diplomonad flagellate Hexamita capsularis sp.nov.from the posterior intestine of the fish(Xenocypris dividi)from Liangzi Lake,Hubei Province,is described.The flagellate was oval,withsmooth surface and homogenous cytoplasm,6.21±0.28μm in length,3.43±0.48μm in width and with anelliptical capsule.Two recurrent flagellae arranged between two nuclei ran longitudinally to the posteriorend.Fibrillar bands connected the accessory organelles of two cytostomal tubes,the body depressed atthe posterior end of the body.
基金the"National Key Basic Research Development Programming"Project of China(No.2001CB610703).
文摘The mechanical properties of several kinds of coal gangue calcined with limestone were Researched so as to find the optimum way of calcinations with limestone. Microstructure and property of hydration process of cement pastes containing added-calcium coal gangue were analyzed by means of scanning electron microscope （SEM） and method of mercury in trusion poremeasurement （MIP）, etc. The experiment can approve those results： when proper amounst of gypsum and fluorite were taken as mineralizers in the course of calcinations of added-calcium coal gangue, activity of coal gangue can be effectively improved. The results of mechanical property and structural characteristic such as hydration process, hydration product and microstructure etc. of cement pastes containing added-calcium coal gangue are consistent.
