目的检测盆腔器官脱垂(pelvic organ prolapse,POP)患者子宫旁韧带组织中基质代谢相关Ⅰ型胶原(collagen type Ⅰ,COL1)、弹性蛋白(elastin)和基质金属蛋白酶9(matrix metalloproteinase,MMP-9)以及氧化损伤相关8-羟基脱氧...目的检测盆腔器官脱垂(pelvic organ prolapse,POP)患者子宫旁韧带组织中基质代谢相关Ⅰ型胶原(collagen type Ⅰ,COL1)、弹性蛋白(elastin)和基质金属蛋白酶9(matrix metalloproteinase,MMP-9)以及氧化损伤相关8-羟基脱氧鸟苷酸(8-hydroxy-2’-deoxyguanosine urine,8-OHdG)含量的变化,检测人子宫旁韧带成纤维细胞在氧化应激作用下基质代谢相关以上各项指标的变化,为研究氧化应激在POP发生发展中的作用机制提供实验基础和理论依据。方法选择2012年10月至2014年10月在武汉大学人民医院因盆腔脏器脱垂Ⅱ-Ⅳ度(POPⅡ~POPⅣ)行子宫全切术的患者50例(POP组)和因其他良性妇科疾病行子宫切除术患者40例(对照组),采用免疫组化方法检测患者的宫旁韧带(子宫骶韧带、子宫主韧带)组织中COL1、elastin、MMP-9、8-OHdG的表达;过氧化氢(hydrogen peroxide,H2O2)处理人子宫旁韧带成纤维细胞,建立氧化应激细胞模型,Western blot检测该模型细胞中COL1、elastin和MMP-9蛋白表达。结果免疫组化结果显示,与对照组相比,POP患者宫旁韧带组织中COL1、elastin表达均降低,MMP-9和8-OHdG表达升高;Western blot与免疫组化具有相一致的结果。结论氧化应激可以引起宫旁韧带成纤维细胞细胞外基质(extracellular matrix,ECM)成分改变,可能参与POP的发生发展。展开更多
Cardiac extracellular matrices(ECM)play crucial functional roles in cardiac biomechanics.Previous studies have mainly focused on collagen,the major structural ECM in heart wall.The role of elastin in cardiac mechanics...Cardiac extracellular matrices(ECM)play crucial functional roles in cardiac biomechanics.Previous studies have mainly focused on collagen,the major structural ECM in heart wall.The role of elastin in cardiac mechanics,however,is poorly understood.In this study,we investigated the spatial distribution and microstructural morphologies of cardiac elastin in porcine left ventricles.We demonstrated that the epicardial elastin network had location-and depth-dependency,and the overall epicardial elastin fiber mapping showed certain correlation with the helical heart muscle fiber architecture.When compared to the epicardial layer,the endocardial layer was thicker and has a higher elastin-collagen ratio and a denser elastin fiber network;moreover,the endocardial elastin fibers were finer and more wavy than the epicardial elastin fibers,all suggesting various interface mechanics.The myocardial interstitial elastin fibers co-exist with the perimysial collagen to bind the cardiomyocyte bundles;some of the interstitial elastin fibers showed a locally aligned,hinge-like structure to connect the adjacent cardiomyocyte bundles.This collagen-elastin combination reflects an optimal design in which the collagen provides mechanical strength and elastin fibers facilitate recoiling during systole.Moreover,cardiac elastin fibers,along with collagen network,closely associated with the Purkinje cells,indicating that this ECM association could be essential in organizing cardiac Purkinje cells into“fibrous”and“branching”morphologies and serving as a protective feature when Purkinje fibers experience large deformations in vivo.In short,our observations provide a structural basis for future in-depth biomechanical investigations and biomimicking of this long-overlooked cardiac ECM component.展开更多
基金support:R01EB022018,R15HL140503,T32 HL134613 from NIH,UT STARS.
文摘Cardiac extracellular matrices(ECM)play crucial functional roles in cardiac biomechanics.Previous studies have mainly focused on collagen,the major structural ECM in heart wall.The role of elastin in cardiac mechanics,however,is poorly understood.In this study,we investigated the spatial distribution and microstructural morphologies of cardiac elastin in porcine left ventricles.We demonstrated that the epicardial elastin network had location-and depth-dependency,and the overall epicardial elastin fiber mapping showed certain correlation with the helical heart muscle fiber architecture.When compared to the epicardial layer,the endocardial layer was thicker and has a higher elastin-collagen ratio and a denser elastin fiber network;moreover,the endocardial elastin fibers were finer and more wavy than the epicardial elastin fibers,all suggesting various interface mechanics.The myocardial interstitial elastin fibers co-exist with the perimysial collagen to bind the cardiomyocyte bundles;some of the interstitial elastin fibers showed a locally aligned,hinge-like structure to connect the adjacent cardiomyocyte bundles.This collagen-elastin combination reflects an optimal design in which the collagen provides mechanical strength and elastin fibers facilitate recoiling during systole.Moreover,cardiac elastin fibers,along with collagen network,closely associated with the Purkinje cells,indicating that this ECM association could be essential in organizing cardiac Purkinje cells into“fibrous”and“branching”morphologies and serving as a protective feature when Purkinje fibers experience large deformations in vivo.In short,our observations provide a structural basis for future in-depth biomechanical investigations and biomimicking of this long-overlooked cardiac ECM component.