A new method for the determination of antithrombotic activity of egg white protein hydrolysate （EWPH） was developed using a microplate reader. Reaction was carried out at 37℃and pH 7.2 with fibrinogen concentration...A new method for the determination of antithrombotic activity of egg white protein hydrolysate （EWPH） was developed using a microplate reader. Reaction was carried out at 37℃and pH 7.2 with fibrinogen concentration 0.1%. Microplate reading was conducted at 405 nm. Inhibition rate of EWPH on thrombin activity showed linearity （R2 = 0.9971）, when the inhibition rate was in the range of 10-90%. The lower limit of detection （LLD, at 99.7% probability） and the biological limit of detection （BLD, at 99.7% probability） of the method were 10.643 and 40 mg/mL, respectively. The repeatability standard deviation （R.S.D.） was 1.08%. The standard deviation of the method was ±0.027 AT-U.展开更多
建立高脂小鼠模型,以辛伐他汀和市售醋蛋液水解物作为药品和食品阳性对照组,综合评价肽酶433P酶解蛋清获得蛋清水解物(egg white hydrolysate,EWH)的体内降血脂效果。结果表明,EWH可以显著抑制小鼠体质量增长,调节血脂异常,减轻肝脏脂...建立高脂小鼠模型,以辛伐他汀和市售醋蛋液水解物作为药品和食品阳性对照组,综合评价肽酶433P酶解蛋清获得蛋清水解物(egg white hydrolysate,EWH)的体内降血脂效果。结果表明,EWH可以显著抑制小鼠体质量增长,调节血脂异常,减轻肝脏脂质异常与炎症损伤,抑制肝脏脂肪细胞的积累、变性和脂肪组织甘油三酯的堆积。之后研究了胃肠消化对EWH血脂调节活性影响,结果发现,经胃肠消化后EWH的胆固醇胶束溶解度抑制率下降,但胆汁酸结合率和胰脂肪酶抑制率基本无明显变化。最后通过质谱鉴定及分子对接,得到了3个潜在的胰脂肪酶抑制活性肽,其氨基酸序列分别为LWVPSVY、YPILPEYLQ和WNIPIGTL,上述肽段可以通过氢键、疏水相互作用和静电相互作用等作用力与受体蛋白1ETH相互作用,从而发挥降血脂作用。展开更多
文摘A new method for the determination of antithrombotic activity of egg white protein hydrolysate （EWPH） was developed using a microplate reader. Reaction was carried out at 37℃and pH 7.2 with fibrinogen concentration 0.1%. Microplate reading was conducted at 405 nm. Inhibition rate of EWPH on thrombin activity showed linearity （R2 = 0.9971）, when the inhibition rate was in the range of 10-90%. The lower limit of detection （LLD, at 99.7% probability） and the biological limit of detection （BLD, at 99.7% probability） of the method were 10.643 and 40 mg/mL, respectively. The repeatability standard deviation （R.S.D.） was 1.08%. The standard deviation of the method was ±0.027 AT-U.
文摘建立高脂小鼠模型,以辛伐他汀和市售醋蛋液水解物作为药品和食品阳性对照组,综合评价肽酶433P酶解蛋清获得蛋清水解物(egg white hydrolysate,EWH)的体内降血脂效果。结果表明,EWH可以显著抑制小鼠体质量增长,调节血脂异常,减轻肝脏脂质异常与炎症损伤,抑制肝脏脂肪细胞的积累、变性和脂肪组织甘油三酯的堆积。之后研究了胃肠消化对EWH血脂调节活性影响,结果发现,经胃肠消化后EWH的胆固醇胶束溶解度抑制率下降,但胆汁酸结合率和胰脂肪酶抑制率基本无明显变化。最后通过质谱鉴定及分子对接,得到了3个潜在的胰脂肪酶抑制活性肽,其氨基酸序列分别为LWVPSVY、YPILPEYLQ和WNIPIGTL,上述肽段可以通过氢键、疏水相互作用和静电相互作用等作用力与受体蛋白1ETH相互作用,从而发挥降血脂作用。
