AIM To study the activity of ecdysterone from Achyranthes bidentata Bl. (AB) promoting proliferation of osteoblast like (OB like) UMR106 cells and to determine its content in AB by HPLC method. METHODS Ecdysterone iso...AIM To study the activity of ecdysterone from Achyranthes bidentata Bl. (AB) promoting proliferation of osteoblast like (OB like) UMR106 cells and to determine its content in AB by HPLC method. METHODS Ecdysterone isolated from AB was cultured with OB like cells UMR106 together in vitro and the proliferation of OB like cells was determined by MTT assay. The chromatographic conditions for determining ecdysterone included an ODS column (250 mm×4 6 mm, 5 μm), a mobile phase consisting of a mixture of water acetontrile tetrahydrofuran (86∶11∶3), detection wavelength of 243 nm, and column temperature of 27℃. Phenacetin was used as the internal standard. RESULTS The ecdysterone from AB had significant activity promoting proliferation of OB like cells, the proliferation was promoted by 41% ( n =3). The average recovery of ecdysterone was 96 2% (RSD=2 1%), the calibration was linear in the range of 30～300 μg·mL -1 (γ=0 9998). CONCLUSION Ecdysterone was screened quickly by cultivating with OB like cells together in vitro . The HPLC method is accurate, fast and reproducible for the determination of ecdysterone in AB.展开更多
AIM: To set up an assay method for the determination of ecdysterone (EDS) in the root of Achyranthes bidentata Bl. by RP HPLC. METHODS: The EDS was separated on a Hypersil ODS column (200 mm×4 6 mm ID, 5 μm), us...AIM: To set up an assay method for the determination of ecdysterone (EDS) in the root of Achyranthes bidentata Bl. by RP HPLC. METHODS: The EDS was separated on a Hypersil ODS column (200 mm×4 6 mm ID, 5 μm), using acetonitrile — water (1 0∶5 4) as the mobile phase and detected at 243 nm. The flow rate was 1 0 mL·min -1 . Phenol was used as an internal standard. RESULTS: The calibration curve was in good linearity over the range of 28 04～280 40 μg (γ=0 9997). The average recovery was 95 2%. CONCLUSION: The method is simple, fast, sensitive and is suitable for quantitative analysis of EDS in the root of Achyranthes bidentata Bl.展开更多
文摘AIM To study the activity of ecdysterone from Achyranthes bidentata Bl. (AB) promoting proliferation of osteoblast like (OB like) UMR106 cells and to determine its content in AB by HPLC method. METHODS Ecdysterone isolated from AB was cultured with OB like cells UMR106 together in vitro and the proliferation of OB like cells was determined by MTT assay. The chromatographic conditions for determining ecdysterone included an ODS column (250 mm×4 6 mm, 5 μm), a mobile phase consisting of a mixture of water acetontrile tetrahydrofuran (86∶11∶3), detection wavelength of 243 nm, and column temperature of 27℃. Phenacetin was used as the internal standard. RESULTS The ecdysterone from AB had significant activity promoting proliferation of OB like cells, the proliferation was promoted by 41% ( n =3). The average recovery of ecdysterone was 96 2% (RSD=2 1%), the calibration was linear in the range of 30～300 μg·mL -1 (γ=0 9998). CONCLUSION Ecdysterone was screened quickly by cultivating with OB like cells together in vitro . The HPLC method is accurate, fast and reproducible for the determination of ecdysterone in AB.
文摘AIM: To set up an assay method for the determination of ecdysterone (EDS) in the root of Achyranthes bidentata Bl. by RP HPLC. METHODS: The EDS was separated on a Hypersil ODS column (200 mm×4 6 mm ID, 5 μm), using acetonitrile — water (1 0∶5 4) as the mobile phase and detected at 243 nm. The flow rate was 1 0 mL·min -1 . Phenol was used as an internal standard. RESULTS: The calibration curve was in good linearity over the range of 28 04～280 40 μg (γ=0 9997). The average recovery was 95 2%. CONCLUSION: The method is simple, fast, sensitive and is suitable for quantitative analysis of EDS in the root of Achyranthes bidentata Bl.
