Objective: To observe expression of SIRT3 in normal liver tissue, cirrhotic tissue and hepatocellular carcinoma (HCC) tissues, and to explore the significance of SIRT3 in primary HCC . Methods: SIRT3 expression was de...Objective: To observe expression of SIRT3 in normal liver tissue, cirrhotic tissue and hepatocellular carcinoma (HCC) tissues, and to explore the significance of SIRT3 in primary HCC . Methods: SIRT3 expression was detected in 10 normal cases, 30 cases with, 30 HCC cases by immunohistochemical and Western-blotting method. Results: Immunohistochemical assay showed that the SIRT3 positive expression rates were 100.0% (10/10), 96.7% (29/30) and 60.0% (18/30), respectively in normal group, paracancer group and HCC group. And the SIRT3 expression in HCC group was significantly lower than in normal group and paracancer group ( P<0.05). Western-blotting showed the SIRT3 expression in cancer tissue was 0.29暲0.07, significantly lower than that in paracancer group and normal group ( P<0.05). SIRT3 expression was related to the differentiation degree and portal vein tumor thrombus ( P<0.05). Conclusions: Abnormal expression of SIRT3 is closely related to the biological behavior of primary HCC.展开更多
基金supported by grants from Medical Science and Technology Development Foundation, Jiangsu Province Department of Health (No.H201013)National Natural Science Foundation of China (No. 81201905)Nature Science Research Grants in University of Jiangsu Province of P.R. China (No.12KJB320009)
文摘Objective: To observe expression of SIRT3 in normal liver tissue, cirrhotic tissue and hepatocellular carcinoma (HCC) tissues, and to explore the significance of SIRT3 in primary HCC . Methods: SIRT3 expression was detected in 10 normal cases, 30 cases with, 30 HCC cases by immunohistochemical and Western-blotting method. Results: Immunohistochemical assay showed that the SIRT3 positive expression rates were 100.0% (10/10), 96.7% (29/30) and 60.0% (18/30), respectively in normal group, paracancer group and HCC group. And the SIRT3 expression in HCC group was significantly lower than in normal group and paracancer group ( P<0.05). Western-blotting showed the SIRT3 expression in cancer tissue was 0.29暲0.07, significantly lower than that in paracancer group and normal group ( P<0.05). SIRT3 expression was related to the differentiation degree and portal vein tumor thrombus ( P<0.05). Conclusions: Abnormal expression of SIRT3 is closely related to the biological behavior of primary HCC.
