以苜蓿(M ed icag o sa tiva)为原材料,添加乳酸菌复合系A l2调制青贮苜蓿,并以不添加接种剂的苜蓿作为对照,分析苜蓿青贮过程中微生物区系变化及接菌的改善效果。通过提取青贮料中细菌的总DNA,通过聚合酶链式反应(PCR)扩增其16S rDNA ...以苜蓿(M ed icag o sa tiva)为原材料,添加乳酸菌复合系A l2调制青贮苜蓿,并以不添加接种剂的苜蓿作为对照,分析苜蓿青贮过程中微生物区系变化及接菌的改善效果。通过提取青贮料中细菌的总DNA,通过聚合酶链式反应(PCR)扩增其16S rDNA V 3区域,利用变性梯度凝胶电泳法(D enaturing G rad ien t G e l E lectrophores is,DGGE)分析青贮料中细菌的区系变化,并结合平板计数法对青贮料中的乳酸菌、一般细菌、霉菌进行动态分析。DGGE图谱结果显示自然青贮苜蓿的优势菌是戊糖片球菌(P ed iococcus p entosaceus)。在接菌A l2的青贮料中,以植物乳杆菌(L actabacillus p lantarum)为主的A l2的成员菌迅速控制发酵过程,并且在发酵过程中稳定存在。与对照相比,添加乳酸菌剂能够提高乳酸菌的数量,在第2 d将青贮料的pH值降低到4.2,最后稳定到4.1,显著提高了乳酸生成量,降低了氨态氮的产生,明显改善了青贮饲料的发酵品质。展开更多
A large proportion of food is composed of crops and meat besides vegetables and fruits.The production of crops and meat needs a large area of land and other resources,such as water,chemical fertilizer,and pesticides.T...A large proportion of food is composed of crops and meat besides vegetables and fruits.The production of crops and meat needs a large area of land and other resources,such as water,chemical fertilizer,and pesticides.The growing world population in creases the demand for food security.Moreover,developi ng countries with highly dense populations have dramatically changed food composition from mostly crops to more meat and milk,which has forced the adjustment and optimization of agricultural production structure to supply the demand caused by these dramatic changes.展开更多
The experiment aimed at determining the nutritional value of integral cassava root silages with yogurt as inoculant or wastewater(manipueira) by liquid addition ensiling process. Eighteen crossbred piglets(Large White...The experiment aimed at determining the nutritional value of integral cassava root silages with yogurt as inoculant or wastewater(manipueira) by liquid addition ensiling process. Eighteen crossbred piglets(Large White x Landrace), castrated males with an initial average weight of 50 kg were allocated in metabolism cages throughout 11-day trial duration(6 days for animal's adaptation to the cages and to the experimental diets, and 5 days of urine and faeces collection). The experimental design was a randomized block arrangement with 3 treatments and 6 replicates: basal diet(100%); mixture composed of75% basal diet and 25% integral cassava root silage with yogurt as inoculant; a mixture composed of 75%of basal diet and 25% integral cassava root silage with wastewater. The silages with wastewater and yogurt presented the following values of apparent digestibility of dry matter 89.96% and 90.01%,apparent digestibility of crude protein of 60.67% and 66.43%, apparent digestibility of gross energy of90.43% and 91.48%, gross energy metabolizability coefficients of 87.88% and 88.93%, digestible energy values of 3,705 and 3,783 kcal/kg on a dry matter(DM) basis, and metabolizable energy values of 3,600 and 3,676 kcal/kg DM, respectively. The results have demonstrated that integral cassava root silages with wastewater or yogurt have a high nutritional value and can be used as an alternative energy source in growing pig's diets.展开更多
文摘以苜蓿(M ed icag o sa tiva)为原材料,添加乳酸菌复合系A l2调制青贮苜蓿,并以不添加接种剂的苜蓿作为对照,分析苜蓿青贮过程中微生物区系变化及接菌的改善效果。通过提取青贮料中细菌的总DNA,通过聚合酶链式反应(PCR)扩增其16S rDNA V 3区域,利用变性梯度凝胶电泳法(D enaturing G rad ien t G e l E lectrophores is,DGGE)分析青贮料中细菌的区系变化,并结合平板计数法对青贮料中的乳酸菌、一般细菌、霉菌进行动态分析。DGGE图谱结果显示自然青贮苜蓿的优势菌是戊糖片球菌(P ed iococcus p entosaceus)。在接菌A l2的青贮料中,以植物乳杆菌(L actabacillus p lantarum)为主的A l2的成员菌迅速控制发酵过程,并且在发酵过程中稳定存在。与对照相比,添加乳酸菌剂能够提高乳酸菌的数量,在第2 d将青贮料的pH值降低到4.2,最后稳定到4.1,显著提高了乳酸生成量,降低了氨态氮的产生,明显改善了青贮饲料的发酵品质。
文摘A large proportion of food is composed of crops and meat besides vegetables and fruits.The production of crops and meat needs a large area of land and other resources,such as water,chemical fertilizer,and pesticides.The growing world population in creases the demand for food security.Moreover,developi ng countries with highly dense populations have dramatically changed food composition from mostly crops to more meat and milk,which has forced the adjustment and optimization of agricultural production structure to supply the demand caused by these dramatic changes.
文摘The experiment aimed at determining the nutritional value of integral cassava root silages with yogurt as inoculant or wastewater(manipueira) by liquid addition ensiling process. Eighteen crossbred piglets(Large White x Landrace), castrated males with an initial average weight of 50 kg were allocated in metabolism cages throughout 11-day trial duration(6 days for animal's adaptation to the cages and to the experimental diets, and 5 days of urine and faeces collection). The experimental design was a randomized block arrangement with 3 treatments and 6 replicates: basal diet(100%); mixture composed of75% basal diet and 25% integral cassava root silage with yogurt as inoculant; a mixture composed of 75%of basal diet and 25% integral cassava root silage with wastewater. The silages with wastewater and yogurt presented the following values of apparent digestibility of dry matter 89.96% and 90.01%,apparent digestibility of crude protein of 60.67% and 66.43%, apparent digestibility of gross energy of90.43% and 91.48%, gross energy metabolizability coefficients of 87.88% and 88.93%, digestible energy values of 3,705 and 3,783 kcal/kg on a dry matter(DM) basis, and metabolizable energy values of 3,600 and 3,676 kcal/kg DM, respectively. The results have demonstrated that integral cassava root silages with wastewater or yogurt have a high nutritional value and can be used as an alternative energy source in growing pig's diets.
