It is undeniable that wireless communication technology has become a very important component of modern society. One aspect of modern society in which application of wireless communication technologies has tremendous ...It is undeniable that wireless communication technology has become a very important component of modern society. One aspect of modern society in which application of wireless communication technologies has tremendous potential is in agricultural production. This is especially true in the area of sensing and transmission of relevant farming information such as weather, crop development, water quantity and quality, among others, which would allow farmers to make more accurate and timely farming decisions. A good example would be the application of wireless communication technology to transmit soil moisture data in real time to help farmers make irrigation scheduling decisions. Although many systems are commercially available for soil moisture monitoring, there are still many important factors, such as cost, limiting widespread adoption of this technology among growers. Our objective in this study was, therefore, to develop and test an affordable wireless communication system for monitoring soil moisture using Decagon EC-5 sensors. The new system uses Arduino-compatible microcontrollers and communication systems to sample and transmit values from four Decagon EC-5 soil moisture sensors. Developing the system required conducting lab calibrations for the EC-5 sensors for the microcontroller operating in either 10-bit or 12-bit analog-to-digital converter (ADC) resolution. The system was successfully tested in the field and reliably collected and transmitted data from a wheat field for more than two months.展开更多
To investigate the efficiency of suicide gene systems on vascular cells, HSV-tk/GCV and EC-CD/5-FC systems were established on vascular endothelial cells in vitro by retroviral transduction. Both modified cell lines w...To investigate the efficiency of suicide gene systems on vascular cells, HSV-tk/GCV and EC-CD/5-FC systems were established on vascular endothelial cells in vitro by retroviral transduction. Both modified cell lines were highly sensitive to prodrugs, the IC50 for GCV was less than 0.4 μM, and IC50 for 5-FC was less than 75 μM,while the parental endothelial cells were insensitive even at the highest concentrations of prodrugs in this experiment. Mixed cellular assay showed that significant bystander effect was exhibited in modified endothelial cells.When only 10% or 30% of the mixed cells were tk positive and exposed to 20 μM GCV for 6 days, more than 60% or 90% of the whole population was killed. Similar result was also found in CD positive cells. These results indicated that both HSV-tk/GCV and EC-CD/5-FC systems could efficiently suppress endothelial cell growth in vitro.展开更多
文摘It is undeniable that wireless communication technology has become a very important component of modern society. One aspect of modern society in which application of wireless communication technologies has tremendous potential is in agricultural production. This is especially true in the area of sensing and transmission of relevant farming information such as weather, crop development, water quantity and quality, among others, which would allow farmers to make more accurate and timely farming decisions. A good example would be the application of wireless communication technology to transmit soil moisture data in real time to help farmers make irrigation scheduling decisions. Although many systems are commercially available for soil moisture monitoring, there are still many important factors, such as cost, limiting widespread adoption of this technology among growers. Our objective in this study was, therefore, to develop and test an affordable wireless communication system for monitoring soil moisture using Decagon EC-5 sensors. The new system uses Arduino-compatible microcontrollers and communication systems to sample and transmit values from four Decagon EC-5 soil moisture sensors. Developing the system required conducting lab calibrations for the EC-5 sensors for the microcontroller operating in either 10-bit or 12-bit analog-to-digital converter (ADC) resolution. The system was successfully tested in the field and reliably collected and transmitted data from a wheat field for more than two months.
文摘To investigate the efficiency of suicide gene systems on vascular cells, HSV-tk/GCV and EC-CD/5-FC systems were established on vascular endothelial cells in vitro by retroviral transduction. Both modified cell lines were highly sensitive to prodrugs, the IC50 for GCV was less than 0.4 μM, and IC50 for 5-FC was less than 75 μM,while the parental endothelial cells were insensitive even at the highest concentrations of prodrugs in this experiment. Mixed cellular assay showed that significant bystander effect was exhibited in modified endothelial cells.When only 10% or 30% of the mixed cells were tk positive and exposed to 20 μM GCV for 6 days, more than 60% or 90% of the whole population was killed. Similar result was also found in CD positive cells. These results indicated that both HSV-tk/GCV and EC-CD/5-FC systems could efficiently suppress endothelial cell growth in vitro.
