目的分析安徽省蚌埠市2021-2022年新报告HIV感染者基因亚型、治疗前耐药特征和HIV分子传播网络情况,为当地制定干预措施提供依据。方法收集蚌埠市2021-2022年新报告HIV感染者样本90份,扩增pol基因并测序,借助Blast HIV sequence databas...目的分析安徽省蚌埠市2021-2022年新报告HIV感染者基因亚型、治疗前耐药特征和HIV分子传播网络情况,为当地制定干预措施提供依据。方法收集蚌埠市2021-2022年新报告HIV感染者样本90份,扩增pol基因并测序,借助Blast HIV sequence database对每条序列进行同源性分析,采用MEGA 7.0构建NJ系统进化树判定所有HIV感染者基因亚型。将所得序列上传至美国斯坦福大学HIV耐药数据库,分析基因型耐药情况。利用Cytoscape实现传播网络的可视化,分析新报告HIV感染者耐药传播特点和网络关联情况。结果蚌埠市85例新报告HIV感染者基因亚型主要为CRF01_AE(58.82%)和CRF07_BC(23.53%),耐药率为10.59%(9/85),主要为非核苷类药物耐药(5/85,5.88%)。在0.5%基因距离阈值下的分子传播网络中,CRF01_AE入网率为44%,存在1个较大的耐药传播簇,耐药位点为L33F;CRF07_BC入网率为10%。分子传播网络中的HIV感染者主要为五河县50岁及以上男性,文化程度较低。结论蚌埠市新报告HIV感染者耐药率较高,形成了耐药传播簇,传播风险较高,应采取有效措施进行干预,减少HIV的传播。展开更多
目的研究安徽省合肥市2020年新报告HIV-1感染者的传播性耐药(transmitted drug resistance,TDR)及分子传播网络情况。方法收集合肥市2020年1—12月新报告HIV-1感染者,扩增pol基因区(2253~3314 nt),判定基因型耐药情况,构建分子传播网络...目的研究安徽省合肥市2020年新报告HIV-1感染者的传播性耐药(transmitted drug resistance,TDR)及分子传播网络情况。方法收集合肥市2020年1—12月新报告HIV-1感染者,扩增pol基因区(2253~3314 nt),判定基因型耐药情况,构建分子传播网络并分析进入网络病例的相关特征。结果成功扩增pol基因区序列376条,主要的毒株为CRF07BC(157,41.8%)和CRF01AE(136,36.2%)。新报告感染者TDR为2.7%(10/376),10例携带监测性耐药突变(surveillance drug resistance mutation,SDRM),并且毒株CRF5501B所占比例较大(4/10)。分别以0.5%作为基因距离阈值构建了分子传播网络,CRF01AE的大型网络以已婚中年男男性行为者(men who have sex with men,MSM)为主,而CRF07BC则以教育水平较高的未婚MSM为主。通过logistic回归分析入网的影响因素发现CD4细胞计数在200~500个/μL者(aOR=3.219,95%CI:1.226~8.452)与CD4细胞计数>500个/μL者(aOR=5.375,95%CI:1.664~17.364)更易进入网络。结论合肥地区2020年新报告HIV-1感染人群以中青年MSM为主,TDR较低,但要加强CRF5501B的耐药监测。展开更多
Background: In African countries, where the burden of neonatal sepsis is the highest, the spread of Carbapenemases Producing Enterobacteriaceae (CPE) in the community, potentially contributing to neonatal mortality, i...Background: In African countries, where the burden of neonatal sepsis is the highest, the spread of Carbapenemases Producing Enterobacteriaceae (CPE) in the community, potentially contributing to neonatal mortality, is a public health concern. The transmission routes are not well defined, particularly the possible key role played by pregnant women. The aim of this study was to understand the neonatal acquisition of CPE in Yaounde, Cameroon. Methods: A transversal analytical study was conducted in an urban area. Maternal stool samples during delivery and the first stool from their new-born were collected and cultured to isolate Enterobacteria. After isolation, characterization using API20E identification system, and antibiotic susceptibility testing were performed according to the Antibiogram Committee of the French Society of Microbiology. Carbapenemases detection was done on each carbapenem-resistant strain using the Modified Hodge Test (MHT) and their classification using the synergy tests with different inhibitors. Results: Out of the 55 CPE isolates identified, Escherichia coli was the most encountered bacteria both in mothers (n = 18, 50.00%) and infants (n = 11, 57.89%). Class B and D carbapenemases were found both in mothers and infants. The estimated prevalence of vertical transmission in our study, was 10% (n = 12). Logistic regression showed that CPE carriage in mothers and CPE acquisition in their new-borns were independently associated with the presence of greenish amniotic fluid (OR = 7.33, p 0.0001 in mothers and OR = 4.09, p = 0.0086 in new-borns). Conclusion: Our results highlight the non-negligeable role played by pregnant women in the neonatal acquisition of CPE.展开更多
文摘目的分析安徽省蚌埠市2021-2022年新报告HIV感染者基因亚型、治疗前耐药特征和HIV分子传播网络情况,为当地制定干预措施提供依据。方法收集蚌埠市2021-2022年新报告HIV感染者样本90份,扩增pol基因并测序,借助Blast HIV sequence database对每条序列进行同源性分析,采用MEGA 7.0构建NJ系统进化树判定所有HIV感染者基因亚型。将所得序列上传至美国斯坦福大学HIV耐药数据库,分析基因型耐药情况。利用Cytoscape实现传播网络的可视化,分析新报告HIV感染者耐药传播特点和网络关联情况。结果蚌埠市85例新报告HIV感染者基因亚型主要为CRF01_AE(58.82%)和CRF07_BC(23.53%),耐药率为10.59%(9/85),主要为非核苷类药物耐药(5/85,5.88%)。在0.5%基因距离阈值下的分子传播网络中,CRF01_AE入网率为44%,存在1个较大的耐药传播簇,耐药位点为L33F;CRF07_BC入网率为10%。分子传播网络中的HIV感染者主要为五河县50岁及以上男性,文化程度较低。结论蚌埠市新报告HIV感染者耐药率较高,形成了耐药传播簇,传播风险较高,应采取有效措施进行干预,减少HIV的传播。
文摘目的研究安徽省合肥市2020年新报告HIV-1感染者的传播性耐药(transmitted drug resistance,TDR)及分子传播网络情况。方法收集合肥市2020年1—12月新报告HIV-1感染者,扩增pol基因区(2253~3314 nt),判定基因型耐药情况,构建分子传播网络并分析进入网络病例的相关特征。结果成功扩增pol基因区序列376条,主要的毒株为CRF07BC(157,41.8%)和CRF01AE(136,36.2%)。新报告感染者TDR为2.7%(10/376),10例携带监测性耐药突变(surveillance drug resistance mutation,SDRM),并且毒株CRF5501B所占比例较大(4/10)。分别以0.5%作为基因距离阈值构建了分子传播网络,CRF01AE的大型网络以已婚中年男男性行为者(men who have sex with men,MSM)为主,而CRF07BC则以教育水平较高的未婚MSM为主。通过logistic回归分析入网的影响因素发现CD4细胞计数在200~500个/μL者(aOR=3.219,95%CI:1.226~8.452)与CD4细胞计数>500个/μL者(aOR=5.375,95%CI:1.664~17.364)更易进入网络。结论合肥地区2020年新报告HIV-1感染人群以中青年MSM为主,TDR较低,但要加强CRF5501B的耐药监测。
文摘Background: In African countries, where the burden of neonatal sepsis is the highest, the spread of Carbapenemases Producing Enterobacteriaceae (CPE) in the community, potentially contributing to neonatal mortality, is a public health concern. The transmission routes are not well defined, particularly the possible key role played by pregnant women. The aim of this study was to understand the neonatal acquisition of CPE in Yaounde, Cameroon. Methods: A transversal analytical study was conducted in an urban area. Maternal stool samples during delivery and the first stool from their new-born were collected and cultured to isolate Enterobacteria. After isolation, characterization using API20E identification system, and antibiotic susceptibility testing were performed according to the Antibiogram Committee of the French Society of Microbiology. Carbapenemases detection was done on each carbapenem-resistant strain using the Modified Hodge Test (MHT) and their classification using the synergy tests with different inhibitors. Results: Out of the 55 CPE isolates identified, Escherichia coli was the most encountered bacteria both in mothers (n = 18, 50.00%) and infants (n = 11, 57.89%). Class B and D carbapenemases were found both in mothers and infants. The estimated prevalence of vertical transmission in our study, was 10% (n = 12). Logistic regression showed that CPE carriage in mothers and CPE acquisition in their new-borns were independently associated with the presence of greenish amniotic fluid (OR = 7.33, p 0.0001 in mothers and OR = 4.09, p = 0.0086 in new-borns). Conclusion: Our results highlight the non-negligeable role played by pregnant women in the neonatal acquisition of CPE.
