Protein kinases are major players in various signal transduction pathways. Understanding the molecular mechanisms behind plant responses to biotic and abiotic stresses has become critical for developing and breeding c...Protein kinases are major players in various signal transduction pathways. Understanding the molecular mechanisms behind plant responses to biotic and abiotic stresses has become critical for developing and breeding climate-resilient crops. In this review,we summarize recent progress on understanding plant drought, salt, and cold stress responses, with a focus on signal perception and transduction by different protein kinases, especially sucrose nonfermenting1(SNF1)-related protein kinases(Sn RKs),mitogen-activated protein kinase(MAPK) cascades,calcium-dependent protein kinases(CDPKs/CPKs),and receptor-like kinases(RLKs). We also discuss future challenges in these research fields.展开更多
DMA cassette containing an AtDREBIA cDNA and a nos terminator, driven by acauliflower mosaic 35S promoter, or a stress-inducible rd29A promoter, was transformed into theground cover chrysanthemum (Dendranthema grandif...DMA cassette containing an AtDREBIA cDNA and a nos terminator, driven by acauliflower mosaic 35S promoter, or a stress-inducible rd29A promoter, was transformed into theground cover chrysanthemum (Dendranthema grandiflorum) ''Fall Color'' genome. Compared with wild typeplants, severe growth retardation was observed in 35S:DREB1A plants, but not in rd29A:DREB1A plants.RT-PCR analysis revealed that, under stress conditions, the DREB1A gene was over-expressedconstitutively in 35S:DREB1A plants, but was over-expressed inductively in rd29A:DREB1A plants. Thetransgenic plants exhibited tolerance to drought and salt stress, and the tolerance wassignificantly stronger in rd29A:DREB1A plants than in 35B:DREB1A plants. Proline content and SODactivity were increased inductively in rd29A:DREB1A plants than in 35S:DREB1A plants under stressconditions. These results indicate that heterologous AtDREBIA can confer drought and salt tolerancein transgenic chrysanthemum, and improvement of the stress tolerance may be related to enhancementof proline content and SOD activity.展开更多
基金supported by grants from the Natural National Science Foundation of China (31730007 and 31921001)the Beijing Outstanding University Discipline Program。
文摘Protein kinases are major players in various signal transduction pathways. Understanding the molecular mechanisms behind plant responses to biotic and abiotic stresses has become critical for developing and breeding climate-resilient crops. In this review,we summarize recent progress on understanding plant drought, salt, and cold stress responses, with a focus on signal perception and transduction by different protein kinases, especially sucrose nonfermenting1(SNF1)-related protein kinases(Sn RKs),mitogen-activated protein kinase(MAPK) cascades,calcium-dependent protein kinases(CDPKs/CPKs),and receptor-like kinases(RLKs). We also discuss future challenges in these research fields.
文摘DMA cassette containing an AtDREBIA cDNA and a nos terminator, driven by acauliflower mosaic 35S promoter, or a stress-inducible rd29A promoter, was transformed into theground cover chrysanthemum (Dendranthema grandiflorum) ''Fall Color'' genome. Compared with wild typeplants, severe growth retardation was observed in 35S:DREB1A plants, but not in rd29A:DREB1A plants.RT-PCR analysis revealed that, under stress conditions, the DREB1A gene was over-expressedconstitutively in 35S:DREB1A plants, but was over-expressed inductively in rd29A:DREB1A plants. Thetransgenic plants exhibited tolerance to drought and salt stress, and the tolerance wassignificantly stronger in rd29A:DREB1A plants than in 35B:DREB1A plants. Proline content and SODactivity were increased inductively in rd29A:DREB1A plants than in 35S:DREB1A plants under stressconditions. These results indicate that heterologous AtDREBIA can confer drought and salt tolerancein transgenic chrysanthemum, and improvement of the stress tolerance may be related to enhancementof proline content and SOD activity.
