We deal with the existence of periodic solutions for doubly degenerate quasilinear parabolic equations of higher order,which can degenerate,on a part of the boundary,on a segment in the interior of the domain and in t...We deal with the existence of periodic solutions for doubly degenerate quasilinear parabolic equations of higher order,which can degenerate,on a part of the boundary,on a segment in the interior of the domain and in time.展开更多
Chronic low back pain and dyskinesia caused by intervertebral disc degeneration(IDD)are seriously aggravated and become more prevalent with age.Current clinical treatments do not restore the biological structure and i...Chronic low back pain and dyskinesia caused by intervertebral disc degeneration(IDD)are seriously aggravated and become more prevalent with age.Current clinical treatments do not restore the biological structure and inherent function of the disc.The emergence of tissue engineering and regenerative medicine has provided new insights into the treatment of IDD.We synthesized biocompatible layered double hydroxide(LDH)nanoparticles and optimized their ion elemental compositions to promote chondrogenic differentiation of human umbilical cord mesenchymal stem cells(hUC-MSCs).The chondrogenic differentiation of LDH-treated MSCs was validated using Alcian blue staining,qPCR,and immunofluorescence analyses.LDH-pretreated hUC-MSCs were differentiated prior to transplantation into the degenerative site of a needle puncture IDD rat model.Repair and regeneration evaluated using X-ray,magnetic resonance imaging,and tissue immunostaining 4-12 weeks after transplantation showed recovery of the disc space height and integrated tissue structure.Transcriptome sequencing revealed significant regulatory roles of the extracellular matrix(ECM)and integrin receptors of focal adhesion signaling pathway in enhancing chondrogenic differentiation and thus prompting tissue regeneration.The construction of ion-specific LDH nanomaterials for in situ intervertebral disc regeneration through the focal adhesion signaling pathway provides theoretical basis for clinical transformation in IDD treatment.展开更多
We deal with the existence of weak solutions of double degenerate quasilinear parabolic inequalities with a Signorini-Dirichlet-Neumann type mixed boundary condition, which may degenerate in certain subset of the boun...We deal with the existence of weak solutions of double degenerate quasilinear parabolic inequalities with a Signorini-Dirichlet-Neumann type mixed boundary condition, which may degenerate in certain subset of the boundary or on a segment in the interior of the domain and in time. The main tools in our study are the maximM monotone property of the derivative operator with zero-initial valued conditions and the theory of pseudomonotone perturbations of maximal monotone mappings.展开更多
基金Project supported partially by NNSF of China Grant No.10171008NSF of Hunan Province Grant No.03JJY3003
文摘We deal with the existence of periodic solutions for doubly degenerate quasilinear parabolic equations of higher order,which can degenerate,on a part of the boundary,on a segment in the interior of the domain and in time.
基金This work was financially supported by the INTERNATIONAL COOPERATION Project of National Natural Science Foundation of China(Grant No.81810001048)the National Natural Science Foundation of China(Grant Nos.81922039,81873994,31727801,82225027 and 82001308)Key Basic Research Projects of Shanghai Science and Technology Commission(Grant No.19JC141470)。
文摘Chronic low back pain and dyskinesia caused by intervertebral disc degeneration(IDD)are seriously aggravated and become more prevalent with age.Current clinical treatments do not restore the biological structure and inherent function of the disc.The emergence of tissue engineering and regenerative medicine has provided new insights into the treatment of IDD.We synthesized biocompatible layered double hydroxide(LDH)nanoparticles and optimized their ion elemental compositions to promote chondrogenic differentiation of human umbilical cord mesenchymal stem cells(hUC-MSCs).The chondrogenic differentiation of LDH-treated MSCs was validated using Alcian blue staining,qPCR,and immunofluorescence analyses.LDH-pretreated hUC-MSCs were differentiated prior to transplantation into the degenerative site of a needle puncture IDD rat model.Repair and regeneration evaluated using X-ray,magnetic resonance imaging,and tissue immunostaining 4-12 weeks after transplantation showed recovery of the disc space height and integrated tissue structure.Transcriptome sequencing revealed significant regulatory roles of the extracellular matrix(ECM)and integrin receptors of focal adhesion signaling pathway in enhancing chondrogenic differentiation and thus prompting tissue regeneration.The construction of ion-specific LDH nanomaterials for in situ intervertebral disc regeneration through the focal adhesion signaling pathway provides theoretical basis for clinical transformation in IDD treatment.
基金Supported by the National Natural Science Foundation of China(Grant No.11271087,No.61263006)Guangxi Scientific Experimental(China-ASEAN Research)Centre No.20120116
文摘We deal with the existence of weak solutions of double degenerate quasilinear parabolic inequalities with a Signorini-Dirichlet-Neumann type mixed boundary condition, which may degenerate in certain subset of the boundary or on a segment in the interior of the domain and in time. The main tools in our study are the maximM monotone property of the derivative operator with zero-initial valued conditions and the theory of pseudomonotone perturbations of maximal monotone mappings.
