A fragment sized 400bp of White spot syndrome virus(WSSV,formerly designated NOSV),recovered from recombinant plasmid pAFD, was labeled with Digoxigenin as a probe to detect dynamic distribution of WSSV within 120h an...A fragment sized 400bp of White spot syndrome virus(WSSV,formerly designated NOSV),recovered from recombinant plasmid pAFD, was labeled with Digoxigenin as a probe to detect dynamic distribution of WSSV within 120h and 72h in crawfishes( Cambarus proclarkii ) inoculated WSSV by oral taking and injection respectively. Stomach epithelium, intestine epithelium, heart, gill, haemolymph, muscle, hepatopancreas, hypoderm, connective tissue and ovary of infected crawfishes were examined for WSSV. In both groups, WSSV was first detected in heamolymph at 12h p.i. and then disappeared. Again it was detected at 96h p.i. only in oral infection group and maintained till 120h p.i., but it didn’t appear at 72h p.i. in injection group. WSSV in heart, muscle was detected at 36h p.i. in oral infection group and 24h p.i. in injection group respectively, and then increased generally. In addition, WSSV in intestine epithelium, connective tissue, ovary of oral infection group and intestine epithelium, hypoderm, ovary of injection group could also be detected. In dead crawfishes after 120h and 72h p.i. in two groups, WSSV could be detected in all the examined tissues and it demonstrated that systemic infection occurred in the animales. The tissue containing more amounts of WSSV was hypoderm in oral infection group, while intestine epithelium, gill, hypoderm, ovary in injection infection group. It deduced that WSSV first appears in haemolymph and then goes into heart, muscle and other tissues and proliferates in them. Once again, WSSV is released into heamolymph resulting in systemic infection till crawfishes’ death.展开更多
作者以人乳头状瘤病毒(HPV)16DNA为探针,采用斑点杂交及Southern分子杂交技术检测24例食管癌及其相应癌旁组织中的HPV16DNA。结果:在食管癌组织中,HPV 16DNA斑点及Southern杂交的阳性率为50％(12/24),癌旁组织为37％(9/24);经Bam H I酶...作者以人乳头状瘤病毒(HPV)16DNA为探针,采用斑点杂交及Southern分子杂交技术检测24例食管癌及其相应癌旁组织中的HPV16DNA。结果:在食管癌组织中,HPV 16DNA斑点及Southern杂交的阳性率为50％(12/24),癌旁组织为37％(9/24);经Bam H I酶切的食管癌及癌旁组织中,杂交后出现7.2kb的HPV16DNA特异性阳性区带;经Pst I酶切杂交后出现2.7kb、2.3kb、1.75kb、1.18kb四条HPV16DNA阳性区带;未经酶切者来发现阳性杂交带出现。提示人乳头状瘤病毒基因组确实存在于食管癌及癌旁组织DNA中,且多以整合状态存在。展开更多
应用异羟基洋地黄毒甙元(digoxigcnin)标记的 DNA 探针,检测了细胞培养物和鸡羽髓中提取的马立克氏病毒(MDV)核酸.结果发现,异羟基洋地黄毒甙元标记的马立克氏病毒血清 I 型(GA 株)核酸的BamHI-L 片段探针,只与马立克氏病毒Ⅰ型核酸发...应用异羟基洋地黄毒甙元(digoxigcnin)标记的 DNA 探针,检测了细胞培养物和鸡羽髓中提取的马立克氏病毒(MDV)核酸.结果发现,异羟基洋地黄毒甙元标记的马立克氏病毒血清 I 型(GA 株)核酸的BamHI-L 片段探针,只与马立克氏病毒Ⅰ型核酸发生杂交,而不与Ⅱ型(SB-1)或Ⅲ型(HVT)发生杂交.对36份鸡羽髓病料提取的病毒核酸的杂交检测与琼扩试验相比较,前者阳性率为94.4%,后者仅为86.1%.由此说明,Ⅰ型病毒的核酸探针可用于区别强毒株和 HVT 疫苗毒株,并可作为马立克氏病的临床检测的有效手段.展开更多
文摘A fragment sized 400bp of White spot syndrome virus(WSSV,formerly designated NOSV),recovered from recombinant plasmid pAFD, was labeled with Digoxigenin as a probe to detect dynamic distribution of WSSV within 120h and 72h in crawfishes( Cambarus proclarkii ) inoculated WSSV by oral taking and injection respectively. Stomach epithelium, intestine epithelium, heart, gill, haemolymph, muscle, hepatopancreas, hypoderm, connective tissue and ovary of infected crawfishes were examined for WSSV. In both groups, WSSV was first detected in heamolymph at 12h p.i. and then disappeared. Again it was detected at 96h p.i. only in oral infection group and maintained till 120h p.i., but it didn’t appear at 72h p.i. in injection group. WSSV in heart, muscle was detected at 36h p.i. in oral infection group and 24h p.i. in injection group respectively, and then increased generally. In addition, WSSV in intestine epithelium, connective tissue, ovary of oral infection group and intestine epithelium, hypoderm, ovary of injection group could also be detected. In dead crawfishes after 120h and 72h p.i. in two groups, WSSV could be detected in all the examined tissues and it demonstrated that systemic infection occurred in the animales. The tissue containing more amounts of WSSV was hypoderm in oral infection group, while intestine epithelium, gill, hypoderm, ovary in injection infection group. It deduced that WSSV first appears in haemolymph and then goes into heart, muscle and other tissues and proliferates in them. Once again, WSSV is released into heamolymph resulting in systemic infection till crawfishes’ death.
文摘作者以人乳头状瘤病毒(HPV)16DNA为探针,采用斑点杂交及Southern分子杂交技术检测24例食管癌及其相应癌旁组织中的HPV16DNA。结果:在食管癌组织中,HPV 16DNA斑点及Southern杂交的阳性率为50％(12/24),癌旁组织为37％(9/24);经Bam H I酶切的食管癌及癌旁组织中,杂交后出现7.2kb的HPV16DNA特异性阳性区带;经Pst I酶切杂交后出现2.7kb、2.3kb、1.75kb、1.18kb四条HPV16DNA阳性区带;未经酶切者来发现阳性杂交带出现。提示人乳头状瘤病毒基因组确实存在于食管癌及癌旁组织DNA中,且多以整合状态存在。
文摘应用异羟基洋地黄毒甙元(digoxigcnin)标记的 DNA 探针,检测了细胞培养物和鸡羽髓中提取的马立克氏病毒(MDV)核酸.结果发现,异羟基洋地黄毒甙元标记的马立克氏病毒血清 I 型(GA 株)核酸的BamHI-L 片段探针,只与马立克氏病毒Ⅰ型核酸发生杂交,而不与Ⅱ型(SB-1)或Ⅲ型(HVT)发生杂交.对36份鸡羽髓病料提取的病毒核酸的杂交检测与琼扩试验相比较,前者阳性率为94.4%,后者仅为86.1%.由此说明,Ⅰ型病毒的核酸探针可用于区别强毒株和 HVT 疫苗毒株,并可作为马立克氏病的临床检测的有效手段.
