We propose an approach to detect the temporary faults induced by an environmental phenomenon called single event upset(SEU). Berger code based self-checking checkers provides an online detection of faults in digital c...We propose an approach to detect the temporary faults induced by an environmental phenomenon called single event upset(SEU). Berger code based self-checking checkers provides an online detection of faults in digital circuits as well as in memory arrays. In this work, a concurrent Berger code based online self-testable architecture is proposed and integrated in 32-bit DLX reduced instruction set computer(RISC) processor on a single silicon chip. The proposed concurrent test methodology is implemented and verified for various arithmetic and logical operations of the DLX processor. The FPGA implementation of the proposed design shows that a meager increase in hardware utilization facilitates online self-testing to detect temporary faults.展开更多
The Chinese sucker,Myxocyprinus asiaticus(M.asiaticus,Catostomidae,Cypriniformes),is the only living species of Catostomidae in Asia.There are more than 75 species of this family in North America.The fossil record of ...The Chinese sucker,Myxocyprinus asiaticus(M.asiaticus,Catostomidae,Cypriniformes),is the only living species of Catostomidae in Asia.There are more than 75 species of this family in North America.The fossil record of this group dates back to the early Eocene.As the Chinese sucker is located at the base of the Cyprinoidei phylogeny,this species is also important in clarifying the evolutionary relationships within Cyprinoidei.Here,we assembled a high-quality genome of the Chinese sucker,contig N50(40.26 Mb),which is nearly ten times longer than the previous version(4.19 Mb).Phylogenetic analysis identified that Chinese sucker together with Cyprinidae groups are paraphyletic with respect to Cobitoidea.The specific whole genome duplication event of the Chinese sucker was estimated to have occurred~25.9 million years ago.Analysis of population historical changes indicated a trend of reduction for the Chinese sucker and T.tibetana.Since Dlx genes play a key role in Cypriniformes pharyngeal teeth development,we conducted a genome-wide identification of Dlx genes,and found that these genes were doubled in whole genome duplication events,followed by the loss of specific copies.Transcriptome results showed that the expression levels of these paralogous genes were similar.This genomic resource provides useful information for the protection of Chinese sucker and functional study of Dlx genes.展开更多
Gastric cancer(GC)was referred to a malignant tumor of the digestive tract originating from the epithelium of gastric mucosa.Transcription factor DLX5 was verified as an oncogene in various types of tumors,while miR-3...Gastric cancer(GC)was referred to a malignant tumor of the digestive tract originating from the epithelium of gastric mucosa.Transcription factor DLX5 was verified as an oncogene in various types of tumors,while miR-376a-3p was speculated as a tumor suppressor.Based on the bioinformatics database,we hypothesized that miR-376a participated in the regulation of GC development by targeting DLX5.Compared with adjacent tissue,a significant increase of DLX5 expression was determined in GC tissues,but the expression level is significantly reduced in miR-376a.Similar expression signature of DLX5 and miR-376a was also determined between 4 GC cells(HGC,SGC,MGC,and AGS cell lines)and GES cell line.The level of DLX5 was notably reduced in HGC and MGC cell lines after miR-376a-3p overexpression,and increased after miR-376a-3p inhibition.Then,the inhibition role of miR-376a-3p on DLX5 was further proved by dualluciferase reporter assay.Gain-of-function experiments showed that upregulation of miR-376a-3p in GC cells could inhibit the ability of epithelial-mesenchymal transition,proliferation,and invasion,and enhance the GC cell apoptosis level.However,these roles of miR-376a-3p could be abolished by DLX5 overexpression.This study confirmed that reduction of miR-376a-3p expression level in GC cells would lead to the increase in cell growth and invasion,indicating that upregulation of miR-376a-3p might have a potential therapeutic role on GC.展开更多
文摘We propose an approach to detect the temporary faults induced by an environmental phenomenon called single event upset(SEU). Berger code based self-checking checkers provides an online detection of faults in digital circuits as well as in memory arrays. In this work, a concurrent Berger code based online self-testable architecture is proposed and integrated in 32-bit DLX reduced instruction set computer(RISC) processor on a single silicon chip. The proposed concurrent test methodology is implemented and verified for various arithmetic and logical operations of the DLX processor. The FPGA implementation of the proposed design shows that a meager increase in hardware utilization facilitates online self-testing to detect temporary faults.
基金supported by grants from the National Natural Science Foundation of China(32170438)to C.FThe research was supported by the Wuhan Branch,Supercomputing Center,Chinese Academy of Sciences,China.We thank Dr Dengqiang Wang from Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences for help with sampling.
文摘The Chinese sucker,Myxocyprinus asiaticus(M.asiaticus,Catostomidae,Cypriniformes),is the only living species of Catostomidae in Asia.There are more than 75 species of this family in North America.The fossil record of this group dates back to the early Eocene.As the Chinese sucker is located at the base of the Cyprinoidei phylogeny,this species is also important in clarifying the evolutionary relationships within Cyprinoidei.Here,we assembled a high-quality genome of the Chinese sucker,contig N50(40.26 Mb),which is nearly ten times longer than the previous version(4.19 Mb).Phylogenetic analysis identified that Chinese sucker together with Cyprinidae groups are paraphyletic with respect to Cobitoidea.The specific whole genome duplication event of the Chinese sucker was estimated to have occurred~25.9 million years ago.Analysis of population historical changes indicated a trend of reduction for the Chinese sucker and T.tibetana.Since Dlx genes play a key role in Cypriniformes pharyngeal teeth development,we conducted a genome-wide identification of Dlx genes,and found that these genes were doubled in whole genome duplication events,followed by the loss of specific copies.Transcriptome results showed that the expression levels of these paralogous genes were similar.This genomic resource provides useful information for the protection of Chinese sucker and functional study of Dlx genes.
基金This work was supported by the Nature Science Foundation of China(81972320).
文摘Gastric cancer(GC)was referred to a malignant tumor of the digestive tract originating from the epithelium of gastric mucosa.Transcription factor DLX5 was verified as an oncogene in various types of tumors,while miR-376a-3p was speculated as a tumor suppressor.Based on the bioinformatics database,we hypothesized that miR-376a participated in the regulation of GC development by targeting DLX5.Compared with adjacent tissue,a significant increase of DLX5 expression was determined in GC tissues,but the expression level is significantly reduced in miR-376a.Similar expression signature of DLX5 and miR-376a was also determined between 4 GC cells(HGC,SGC,MGC,and AGS cell lines)and GES cell line.The level of DLX5 was notably reduced in HGC and MGC cell lines after miR-376a-3p overexpression,and increased after miR-376a-3p inhibition.Then,the inhibition role of miR-376a-3p on DLX5 was further proved by dualluciferase reporter assay.Gain-of-function experiments showed that upregulation of miR-376a-3p in GC cells could inhibit the ability of epithelial-mesenchymal transition,proliferation,and invasion,and enhance the GC cell apoptosis level.However,these roles of miR-376a-3p could be abolished by DLX5 overexpression.This study confirmed that reduction of miR-376a-3p expression level in GC cells would lead to the increase in cell growth and invasion,indicating that upregulation of miR-376a-3p might have a potential therapeutic role on GC.
